Standard name
Human Ortholog
Description Transcription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.06 0.06 0.13 0.08 0.09 0.06 0.09 0 0 0 0 0.15 0.19 0.26 0.22 0.15 0.25 0 0 0 0 0.05 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.52 0.12 0.23 0.35 0.33 0.64 0.73 0.55 0.59 0.71 0.74 0.66 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Nucleus 0.74 0.8 0.77 0.74 0.62 0.63 0.6 0.66 0.7 0.69 0.63 0.71 0.77 0.78 0.7 0.65 0.58 0.51 0.76 0.74 0.7 0.71 0.59 0.53
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.12 0.06 0 0.1 0.05 0 0 0 0 0 0 0.05 0.05 0.07 0.11 0.24 0.21 0.15 0.12 0.14 0.16 0.23 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Bud 0 1 4 2 2 0 3 2 7 9 9 8 0 0 0 0 2 0 0 3 3 1 5 7
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3
Bud Site 0 0 1 2 1 4 6 7 9 32 15 11 0 0 0 0 0 1
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 10 14 19 18 20 23 31 17 27 18 13 43 81 112 29 37 53 2 8 4 4 18 17
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 0 0 0 0 0 4 7 10 12 0 0 1 0 0 2
Endosome 0 2 3 0 3 0 0 0 0 0 0 1 8 2 3 4 12 6 1 2 5 1 3 13
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 2 0 0 4 0 1 1
Mitochondria 58 21 54 52 72 145 278 179 245 438 420 376 5 1 1 7 15 11 1 2 6 3 6 13
Nucleus 82 138 184 110 134 141 229 217 289 429 361 406 224 327 306 84 145 107 95 147 180 107 207 199
Nuclear Periphery 0 0 0 0 0 0 4 3 0 7 4 5 0 0 0 1 0 0 0 0 1 0 0 1
Nucleolus 0 0 1 1 4 4 4 6 10 29 28 26 1 0 0 1 1 0 1 0 2 0 10 16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 3 1 6 0 3 2 5 6 3 5 4 1 6 5 3 9 12
Vac/Vac Membrane 7 20 14 6 22 12 7 11 9 12 12 18 15 22 29 14 59 44 19 22 36 23 79 80
Unique Cell Count 111 172 239 148 215 225 383 328 412 621 569 568 290 420 437 130 251 209 126 199 259 151 351 379
Labelled Cell Count 152 194 276 193 257 326 554 460 587 989 867 867 298 438 461 150 288 240 126 199 259 151 351 379


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 5.2 4.5 4.5 4.5 3.9 3.8 4.0 4.0 3.6 3.4 3.6 5.9 5.8 5.7 6.3 6.0 5.8 6.1 6.0 6.4
Std Deviation (1e-4) 0.4 1.4 1.0 1.7 1.8 1.5 1.4 1.7 1.3 1.2 1.1 1.0 1.1 1.2 1.4 1.4 1.3 1.6 1.2 1.0 1.4
Intensity Change (Log2) -0.02 -0.02 -0.22 -0.26 -0.16 -0.19 -0.32 -0.39 -0.31 0.39 0.36 0.33 0.48 0.41 0.37 0.43 0.42 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 1.0 1.3 0.1 1.6 -1.0 -0.9 -1.8 -2.6 3.3 4.7 6.3 4.7 3.2 5.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -7.6 -10.0 -10.2 -4.3 -5.3 -5.2
Nucleus -0.6 -3.4 -3.4 -4.4 -2.8 -1.9 -2.3 -3.7 -1.6 0.1 0.3 -1.9 -2.5 -4.5 -5.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.8 1.7 -0.2 -2.7 -1.4 -2.4 -3.0 -2.8 -1.8 -0.3 -0.3 0.4 1.7 5.5 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2672 2.8717 2.7839 2.625 2.4881 2.9075 0.0916 0.5505 0.5272 0.0429 0.3316 0.3159 -0.3684 -0.348 -0.4525 -0.2885 -0.0964 -0.5043
Actin 0 0.0001 0.0002 0.0004 0.0001 0.0004 0.0199 0.0001 0.0105 0.0002 0.0295 0.0019 0.0263 0.0001 0.0267 0.0034 0.0334 0.0009
Bud 0 0.0001 0 0.0001 0 0.0001 0.0006 0.0001 0.0004 0 0.0034 0.0001 0.0006 0.0001 0.0002 0.0008 0.0029 0.0004
Bud Neck 0.0003 0.0003 0.0003 0.0007 0.0003 0.0031 0.0017 0.0002 0.0004 0.0001 0.0012 0.0018 0.0019 0.0002 0.0005 0.0009 0.0031 0.0048
Bud Periphery 0 0 0 0.0001 0 0.0002 0.0013 0.0001 0.0005 0 0.0077 0.0001 0.0012 0.0001 0.0003 0.0027 0.0045 0.0015
Bud Site 0.0002 0.0005 0.0002 0.0004 0.0001 0.0004 0.0014 0.0004 0.0007 0.0001 0.0104 0.0001 0.0051 0.0004 0.0007 0.0012 0.0291 0.0007
Cell Periphery 0 0 0 0 0 0 0.0002 0 0 0 0.0004 0 0.0003 0 0.0001 0.0002 0.0005 0.0002
Cytoplasm 0.011 0.0256 0.0196 0.0225 0.0093 0.0147 0.0044 0.0159 0.0096 0.0028 0.0098 0.0026 0.0118 0.026 0.0066 0.0093 0.0218 0.0063
Cytoplasmic Foci 0.0016 0.0006 0.0008 0.0011 0.0001 0.001 0.0076 0.0002 0.0033 0.0004 0.002 0.0026 0.0074 0.0009 0.0036 0.0132 0.044 0.0005
Eisosomes 0 0 0.0001 0 0 0 0.0002 0 0 0 0.0002 0 0.0002 0 0.0002 0.0001 0.0004 0
Endoplasmic Reticulum 0.0005 0.003 0.001 0.0007 0.0001 0.0003 0.0014 0.0006 0.0005 0.0001 0.004 0.0003 0.0018 0.0022 0.0016 0.0015 0.0029 0.0004
Endosome 0.0004 0.0034 0.0009 0.0008 0.0001 0.0011 0.0385 0.0011 0.007 0.0005 0.0219 0.0044 0.0153 0.0014 0.0029 0.0466 0.0566 0.0031
Golgi 0 0.0001 0 0 0 0.001 0.0103 0 0.0035 0.0001 0.0052 0.0074 0.0071 0 0.0029 0.015 0.0117 0.0009
Lipid Particles 0.0001 0.0002 0.0004 0.0003 0 0.0034 0.0101 0 0.0006 0.0001 0.0033 0.0037 0.0116 0.0003 0.005 0.0139 0.0205 0.0031
Mitochondria 0.0001 0.0005 0.0005 0.0007 0.0003 0.0011 0.0193 0.0004 0.001 0.0001 0.0088 0.0031 0.0129 0.0003 0.0024 0.0266 0.0107 0.0013
None 0.0371 0.0712 0.046 0.058 0.2089 0.0359 0.0011 0.0059 0.0055 0.0008 0.0024 0.0005 0.0045 0.0356 0.002 0.0016 0.0475 0.0056
Nuclear Periphery 0.0025 0.0269 0.0131 0.0146 0.0034 0.0039 0.0082 0.013 0.0089 0.0067 0.0567 0.0046 0.0188 0.0222 0.013 0.0206 0.0141 0.0144
Nucleolus 0.0064 0.0058 0.0098 0.0067 0.0069 0.015 0.0049 0.0045 0.0038 0.0025 0.0072 0.0077 0.0051 0.0038 0.0046 0.0056 0.0036 0.0107
Nucleus 0.9117 0.8482 0.8727 0.8585 0.7631 0.8885 0.8508 0.9476 0.9234 0.9612 0.812 0.9436 0.8376 0.8989 0.9092 0.802 0.6503 0.9267
Peroxisomes 0 0 0 0.0001 0 0.0019 0.0057 0 0.001 0.0001 0.0011 0.001 0.0022 0 0.0034 0.004 0.0209 0.0002
Punctate Nuclear 0.0273 0.0103 0.0335 0.032 0.0068 0.0273 0.0054 0.0073 0.0187 0.024 0.0047 0.0137 0.0239 0.0057 0.0133 0.0218 0.0073 0.0164
Vacuole 0.0006 0.002 0.0005 0.0016 0.0002 0.0005 0.0043 0.002 0.0004 0.0001 0.0039 0.0004 0.0027 0.0014 0.0005 0.0056 0.0102 0.0013
Vacuole Periphery 0 0.0012 0.0002 0.0006 0.0001 0.0001 0.0026 0.0006 0.0002 0.0001 0.0042 0.0003 0.0015 0.0004 0.0003 0.0034 0.004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.7537 8.0911 6.5497 6.6531 10.1348 10.6908 10.412 10.5411 8.1226 10.7614
Translational Efficiency 0.477 1.1531 0.7655 0.6772 0.5576 0.4899 0.6165 0.5675 0.6897 0.4322

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Transcription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole
Localization
Cell Percentages nucleus (85%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Rds2

Rds2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rds2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available