Standard name | |
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Human Ortholog | |
Description | Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 17 | 1 | 4 | 0 | 0 | 1 | – | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 3 | 6 | – | 1 | 36 | 28 | 9 | 14 | 18 | 20 |
Bud | 4 | 1 | 1 | 1 | 1 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | – | 0 | 4 | 1 | 1 | 1 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 10 | 2 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 2 | 1 | 1 | 3 | 2 | – | 1 | 5 | 7 | 7 | 5 | 1 | 1 | 2 | 9 | – | 3 | 1 | 1 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 5 | 2 | 0 | 1 | 1 | 5 | – | 2 | 3 | 1 | 0 | 2 | 0 | 1 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 5 | 0 | 1 | 0 | 1 | 1 |
Golgi | 68 | 34 | 1 | 0 | 0 | 1 | – | 2 | 1 | 0 | 3 | 1 | 3 | 7 | 7 | 35 | – | 5 | 137 | 88 | 26 | 47 | 41 | 49 |
Mitochondria | 713 | 276 | 105 | 21 | 44 | 90 | – | 178 | 165 | 167 | 122 | 142 | 223 | 128 | 169 | 7 | – | 5 | 680 | 228 | 71 | 95 | 75 | 110 |
Nucleus | 4 | 0 | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 3 | 1 | 1 | 0 | 0 | 0 | – | 1 | 2 | 1 | 3 | 6 | 0 | 0 | 0 | 0 | – | 0 | 3 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 90 | 41 | 16 | 3 | 18 | 22 | – | 39 | 40 | 31 | 21 | 30 | 29 | 25 | 10 | 16 | – | 18 | 20 | 11 | 3 | 4 | 11 | 9 |
Peroxisomes | 4 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 7 | 5 | 1 | 5 | 6 | 3 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 89 | 61 | 3 | 0 | 3 | 5 | – | 8 | 2 | 4 | 0 | 1 | 18 | 18 | 15 | 24 | – | 7 | 30 | 8 | 1 | 3 | 4 | 6 |
Unique Cell Count | 830 | 325 | 108 | 22 | 48 | 97 | 192 | 176 | 180 | 138 | 155 | 230 | 147 | 178 | 87 | 35 | 944 | 385 | 120 | 180 | 163 | 209 | ||
Labelled Cell Count | 1012 | 419 | 132 | 26 | 69 | 124 | 231 | 217 | 213 | 164 | 189 | 276 | 182 | 207 | 104 | 43 | 944 | 385 | 120 | 180 | 163 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 43.9 | 90.8 | 39.4 | 54.6 | 60.8 | 42.7 | – | 35.0 | 33.0 | 29.9 | 30.2 | 32.2 | 63.7 | 67.9 | 66.7 | 68.7 | – | 68.6 | 73.6 | 75.4 | 78.8 |
Std Deviation (1e-4) | 14.9 | 29.6 | 14.9 | 23.5 | 25.9 | 20.0 | – | 14.2 | 14.8 | 13.4 | 14.2 | 16.7 | 19.7 | 25.1 | 25.0 | 34.2 | – | 35.2 | 28.4 | 32.4 | 34.6 |
Intensity Change (Log2) | – | – | – | 0.47 | 0.62 | 0.11 | – | -0.17 | -0.26 | -0.4 | -0.38 | -0.29 | 0.69 | 0.78 | 0.76 | 0.8 | – | 0.8 | 0.9 | 0.94 | 1.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7.0 | – | 0 |
Mitochondria | -0.4 | -1.5 | -1.5 | – | -1.6 | -1.3 | -1.6 | -2.6 | -1.9 | -0.1 | -2.9 | -0.9 | -12.5 | – | -10.1 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleolus | 0 | 3.2 | 1.4 | – | 1.2 | 1.6 | 0.5 | 0.1 | 1.0 | -0.6 | 0.5 | -2.6 | 0.7 | – | 4.4 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.7 | 1.9 | 5.0 | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 186.2067 | 207.5609 | 190.8843 | 69.1482 | 202.3676 | 194.4317 | 138.3862 | 168.9697 | 152.2411 | 151.8637 | 174.9399 | 147.0866 | 166.6729 | 159.2805 | 146.8431 | 126.4266 | 159.3858 | 145.2527 |
Actin | 0.0199 | 0.0004 | 0.001 | 0.0004 | 0.0016 | 0.0001 | 0.022 | 0.0004 | 0.0011 | 0.0002 | 0 | 0.0043 | 0.0002 | 0.0004 | 0.0005 | 0 | 0.0001 | 0 |
Bud | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0004 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 |
Bud Periphery | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0015 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0 | 0.0006 | 0.0002 | 0.0002 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0.0002 | 0.0009 | 0.0005 | 0.0002 | 0.0004 | 0.0004 | 0.0012 | 0.0008 | 0.0003 | 0 | 0.0003 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0109 | 0 | 0 | 0.0007 | 0 | 0 | 0.0005 | 0 | 0.0006 | 0 | 0 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.005 | 0 | 0.0024 | 0.0542 | 0.0005 | 0 | 0.0223 | 0.0001 | 0.003 | 0 | 0 | 0.0012 | 0.0017 | 0.0001 | 0.0002 | 0 | 0 | 0 |
Eisosomes | 0.0032 | 0.0019 | 0.0124 | 0.0017 | 0.0005 | 0.007 | 0.0026 | 0.0043 | 0.0146 | 0.002 | 0.0004 | 0.0099 | 0.0039 | 0.0021 | 0.0093 | 0.0056 | 0.0049 | 0.0003 |
Endoplasmic Reticulum | 0.0006 | 0 | 0.0002 | 0.0005 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0021 | 0 | 0.0019 | 0.048 | 0.0012 | 0 | 0.0102 | 0 | 0.0005 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.0018 | 0.0001 | 0.001 | 0.0083 | 0.0013 | 0.0001 | 0.0071 | 0.0001 | 0.0008 | 0.0002 | 0 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Lipid Particles | 0.0144 | 0.0026 | 0.0115 | 0.158 | 0.0021 | 0.0008 | 0.0256 | 0.004 | 0.0044 | 0.003 | 0.0008 | 0.0101 | 0.0025 | 0.0045 | 0.0112 | 0.0004 | 0.0001 | 0.013 |
Mitochondria | 0.9171 | 0.9874 | 0.9506 | 0.6085 | 0.981 | 0.9851 | 0.8497 | 0.9552 | 0.9379 | 0.9872 | 0.9967 | 0.9531 | 0.9659 | 0.964 | 0.9486 | 0.993 | 0.9935 | 0.9839 |
None | 0.0008 | 0 | 0.0002 | 0.0034 | 0 | 0 | 0.0016 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0012 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0015 | 0 | 0.0015 | 0.0017 | 0 | 0 | 0.0018 | 0 | 0.0027 | 0 | 0 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.0036 | 0.0017 | 0.0017 | 0.0793 | 0.0011 | 0.0022 | 0.0236 | 0.0202 | 0.0126 | 0.0002 | 0.0001 | 0.0054 | 0.0128 | 0.0143 | 0.016 | 0 | 0 | 0.0001 |
Nucleus | 0.0014 | 0.0001 | 0.0002 | 0.0025 | 0.0002 | 0.0002 | 0.0012 | 0.0009 | 0.0063 | 0.0001 | 0 | 0.0004 | 0.0007 | 0.0005 | 0.0007 | 0 | 0 | 0 |
Peroxisomes | 0.0139 | 0.0019 | 0.004 | 0.0125 | 0.0038 | 0.0004 | 0.0148 | 0.0019 | 0.0045 | 0.0003 | 0.0003 | 0.0052 | 0.0012 | 0.0017 | 0.0018 | 0.0004 | 0.0011 | 0.0003 |
Punctate Nuclear | 0.0004 | 0 | 0.0001 | 0.0031 | 0 | 0 | 0.003 | 0 | 0.0002 | 0 | 0 | 0.0008 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.0006 | 0.0003 | 0.0014 | 0.0015 | 0.0009 | 0.0004 | 0.0014 | 0.0028 | 0.002 | 0.0004 | 0.0001 | 0.0005 | 0.0008 | 0.0009 | 0.0004 | 0 | 0 | 0 |
Vacuole Periphery | 0.0019 | 0.0031 | 0.0086 | 0.0149 | 0.0025 | 0.0032 | 0.0105 | 0.0085 | 0.0075 | 0.0059 | 0.0016 | 0.0068 | 0.0077 | 0.0108 | 0.0105 | 0.0005 | 0.0002 | 0.0022 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 89.7347 | 81.2077 | 69.0781 | 75.8413 | 73.2542 | 190.4423 | 142.6894 | 133.8143 | 177.7999 | 134.641 |
Translational Efficiency | 1.8859 | 2.1599 | 1.9506 | 2.2749 | 1.9415 | 1.9333 | 1.8071 | 2.0248 | 1.6608 | 1.8022 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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