Standard name
Human Ortholog
Description Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; promotes meiotic DSB formation by interacting with H3K4me3 and Rec107p, a protein required for Spo11p-catalyzed DSB formation located on chromosome axes; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.1 0.05 0.05 0.06 0.07 0.07 0 0 0.07 0.16 0.16 0.11 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.37 0 0.05 0.07 0.05 0.29 0.53 0.37 0.67 0.53 0.74 0.49 0 0 0 0.06 0.05 0.07 0 0 0 0 0 0
Nucleus 0.91 0.93 0.94 0.9 0.84 0.82 0.73 0.76 0.69 0.69 0.64 0.74 0.91 0.83 0.82 0.8 0.82 0.86 0.74 0.82 0.71 0.75 0.68 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.09 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0 0.06 0.15 0.08 0.11 0.12 0.17 0.16
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.07 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 3 9 2 4 6 3 0 0 0 0 0 0 0 2 2 3 3 4
Bud Neck 0 0 2 0 0 1 1 4 0 2 2 1 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 1 0 0 0 1 4 5 6 10 11 15 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 6 7 12 28 12 16 20 18 23 10 6 18 33 37 22 26 24 1 0 2 2 1 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 6 5 0 0 0 0 0 1
Endosome 0 1 1 3 3 4 0 0 0 0 1 1 0 0 2 1 1 2 4 3 1 1 1 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1 0 0 0 1 0
Mitochondria 81 11 11 16 16 76 179 123 184 185 182 123 6 1 0 11 13 21 2 2 5 0 1 3
Nucleus 199 229 220 197 248 212 249 255 191 244 157 187 221 171 186 159 197 267 166 210 180 164 191 165
Nuclear Periphery 1 0 0 0 0 1 0 0 0 2 0 0 0 0 1 1 1 1 1 0 1 0 1 0
Nucleolus 31 21 1 0 0 4 12 4 13 16 13 5 5 2 1 5 9 18 34 19 28 26 48 44
Peroxisomes 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0
SpindlePole 2 0 8 2 10 7 1 15 6 3 3 6 7 5 1 1 0 1 3 3 5 3 7 18
Vac/Vac Membrane 1 6 2 2 7 5 5 3 2 2 2 4 2 1 1 6 10 9 5 11 22 14 21 21
Unique Cell Count 219 247 234 218 294 260 340 335 276 352 245 253 244 206 228 199 241 310 224 257 253 218 283 275
Labelled Cell Count 319 274 252 233 315 327 471 440 426 492 391 344 259 214 229 211 264 349 224 257 253 218 283 275


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 6.5 5.7 5.5 5.6 4.4 4.1 4.4 3.8 4.2 3.6 4.1 5.9 6.4 6.5 8.1 7.9 7.5 6.5 6.5 6.9
Std Deviation (1e-4) 0.6 1.0 1.1 1.5 1.7 1.2 1.1 1.2 1.1 1.4 1.1 0.9 1.3 1.7 1.4 1.7 1.6 1.8 1.5 1.7 2.0
Intensity Change (Log2) – – – -0.06 -0.04 -0.38 -0.49 -0.37 -0.58 -0.45 -0.66 -0.48 0.05 0.16 0.18 0.51 0.47 0.39 0.18 0.18 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 3.0 0.9 1.0 1.6 1.8 1.9 0.6 -0.4 2.2 4.7 4.8 3.3 3.3 2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.2 0.4 0 0 0 0 0 0 0 -1.3 -2.7 -3.3 0.4 0.3 1.0
Nucleus -1.5 -3.5 -4.2 -6.3 -5.7 -7.1 -7.2 -8.0 -6.0 -1.4 -3.7 -4.1 -4.4 -4.1 -3.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 -0.3 -0.6 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6338 2.4992 2.3796 2.1732 2.3197 2.2893 1.3014 1.3509 1.3622 1.1784 1.0459 1.1698 0.3158 0.3275 0.3912 1.5726 1.2596 0.1344
Actin 0.0181 0.0001 0.009 0.0022 0.0389 0.0065 0.0025 0 0.0031 0 0.0023 0.0032 0.0084 0.0019 0.0057 0.0659 0.032 0.0098
Bud 0.0012 0 0.0003 0.0003 0.0011 0.0006 0.0001 0 0 0 0.0001 0 0.0004 0.0001 0.0009 0.0066 0.0022 0.0001
Bud Neck 0.0061 0.0001 0.0011 0.0015 0.001 0.0015 0.0004 0.0001 0.0004 0.0001 0.0006 0.0006 0.0049 0.0002 0.0005 0.0122 0.0018 0.0011
Bud Periphery 0.0038 0 0.0007 0.0006 0.0027 0.0014 0.0002 0 0 0 0.0002 0.0001 0.0005 0.0002 0.001 0.03 0.0031 0.0001
Bud Site 0.0052 0.0002 0.0012 0.0108 0.0014 0.0019 0.0002 0 0.0001 0 0.0001 0.0001 0.007 0.0004 0.0055 0.0112 0.0093 0.0001
Cell Periphery 0.0008 0 0.0001 0.001 0.0003 0.0001 0.0001 0 0 0 0 0 0.0002 0 0.0002 0.0069 0.0007 0
Cytoplasm 0.0068 0.0015 0.0046 0.0003 0.0022 0.0004 0.0007 0.0054 0.0001 0.001 0.0004 0.0003 0.0034 0.0001 0.0054 0.0012 0.0068 0.0197
Cytoplasmic Foci 0.0135 0.0001 0.0075 0.0009 0.0092 0.0009 0.0015 0.0001 0 0 0.0003 0.0003 0.0192 0.0004 0.0088 0.0051 0.0268 0.0007
Eisosomes 0.0003 0 0.0001 0.0004 0.0004 0 0 0 0.0001 0 0 0 0.0001 0 0.0001 0.001 0.0002 0
Endoplasmic Reticulum 0.005 0.0001 0.0013 0.0001 0.007 0.0011 0.0006 0.0001 0.0002 0 0.0004 0.0002 0.0005 0 0.0009 0.0044 0.0085 0.0012
Endosome 0.0279 0.0002 0.0126 0.0007 0.0711 0.004 0.0043 0.0003 0.0004 0 0.0017 0.0012 0.0055 0.0003 0.0072 0.0272 0.0941 0.002
Golgi 0.007 0 0.0045 0.0008 0.0133 0.0026 0.0007 0 0.0002 0 0.0003 0.0003 0.0033 0.001 0.0059 0.0167 0.0307 0.0006
Lipid Particles 0.0214 0 0.0079 0.0006 0.0579 0.0008 0.0011 0 0.0001 0 0.0007 0.0004 0.0129 0.0004 0.0033 0.0113 0.0486 0.0001
Mitochondria 0.0112 0.0001 0.0184 0.019 0.0301 0.0053 0.001 0.0001 0.0003 0 0.0013 0.0002 0.0058 0.0075 0.0174 0.0334 0.0099 0.0002
None 0.0043 0.0007 0.0118 0.0002 0.0027 0.0004 0.0005 0.0025 0.0001 0.0007 0.0004 0.0008 0.0018 0 0.0029 0.0017 0.0022 0.0095
Nuclear Periphery 0.0198 0.0029 0.0078 0.0004 0.0253 0.0015 0.0042 0.0024 0.0012 0.0011 0.0403 0.0017 0.0037 0.0007 0.0035 0.0071 0.1071 0.0032
Nucleolus 0.0238 0.0188 0.0143 0.0178 0.053 0.0534 0.0156 0.0175 0.0153 0.0092 0.0463 0.0377 0.0214 0.021 0.0159 0.0153 0.0374 0.0308
Nucleus 0.7802 0.9603 0.8709 0.9392 0.6346 0.9044 0.9577 0.9575 0.9752 0.9826 0.8493 0.9424 0.878 0.9632 0.9077 0.7244 0.5074 0.9129
Peroxisomes 0.0114 0 0.0068 0.0013 0.0109 0.0008 0.0005 0 0 0 0.0002 0.0001 0.0132 0.0008 0.0027 0.0038 0.0074 0.0001
Punctate Nuclear 0.0234 0.0146 0.0159 0.001 0.0168 0.0117 0.0072 0.0137 0.0029 0.0051 0.0542 0.0102 0.0082 0.0016 0.0019 0.0032 0.0105 0.0074
Vacuole 0.0063 0.0001 0.0021 0.0006 0.0117 0.0006 0.0004 0.0003 0.0001 0 0.0003 0.0001 0.0011 0.0001 0.002 0.0079 0.0417 0.0003
Vacuole Periphery 0.0025 0 0.0012 0.0001 0.0083 0.0003 0.0003 0.0001 0.0001 0 0.0007 0.0001 0.0003 0.0001 0.0005 0.0038 0.0115 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9484 12.0743 7.5812 11.1079 12.7974 8.3073 14.3213 13.6273 12.2416 13.478
Translational Efficiency 1.0113 1.2789 1.4218 1.127 0.9597 1.4262 0.8236 0.9546 0.8817 0.8768

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; promotes meiotic DSB formation by interacting with H3K4me3 and Rec107p, a protein required for Spo11p-catalyzed DSB formation located on chromosome axes; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to cytosol in response to hypoxia
Localization
Cell Percentages nucleus (95%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Spp1

Spp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available