ORF | |
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Human Ortholog | |
Description | Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 28 | 58 | 16 | 6 | 28 | 10 | 31 | – | 17 | 15 | 20 | 20 | 60 | 65 | 41 | 46 | 26 | 23 | 86 | 73 | 44 | 54 | 119 | 119 |
Bud | 10 | 20 | 7 | 1 | 10 | 7 | 19 | – | 11 | 16 | 24 | 21 | 31 | 19 | 21 | 4 | 7 | 2 | 3 | 0 | 0 | 3 | 8 | 9 |
Bud Neck | 4 | 4 | 15 | 3 | 10 | 1 | 2 | – | 2 | 3 | 1 | 0 | 1 | 4 | 2 | 5 | 4 | 0 | 22 | 29 | 24 | 31 | 47 | 39 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 1 | 1 | – | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 70 | 85 | 70 | 55 | 52 | 50 | 53 | – | 44 | 45 | 42 | 51 | 137 | 174 | 162 | 42 | 42 | 29 | 166 | 183 | 173 | 230 | 336 | 357 |
Cytoplasm | 316 | 320 | 305 | 228 | 292 | 259 | 330 | – | 208 | 215 | 147 | 168 | 355 | 467 | 554 | 291 | 225 | 164 | 61 | 66 | 64 | 27 | 37 | 31 |
Endoplasmic Reticulum | 8 | 7 | 4 | 0 | 4 | 1 | 2 | – | 5 | 2 | 13 | 5 | 0 | 1 | 2 | 32 | 12 | 12 | 19 | 14 | 13 | 8 | 16 | 19 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 8 | 5 | 1 | 3 | 5 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 4 | 0 | 0 | 2 | 2 |
Mitochondria | 5 | 5 | 0 | 2 | 10 | 44 | 56 | – | 94 | 118 | 120 | 100 | 4 | 0 | 3 | 6 | 4 | 5 | 16 | 25 | 42 | 30 | 39 | 38 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 1 | 3 | 4 | 1 | 1 | 0 | 1 | 5 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 1 | 0 | 10 | 18 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 4 | 4 | 3 | 2 | 7 | 3 | 5 | – | 4 | 1 | 2 | 7 | 3 | 15 | 3 | 15 | 9 | 9 | 1 | 1 | 1 | 0 | 0 | 3 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 1 | 1 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 2 | 4 | 10 | 6 | 6 | 6 | 3 |
Unique Cell Count | 370 | 404 | 345 | 253 | 348 | 299 | 397 | 314 | 336 | 283 | 283 | 438 | 562 | 622 | 375 | 278 | 207 | 400 | 428 | 385 | 404 | 629 | 645 | |
Labelled Cell Count | 447 | 505 | 422 | 297 | 416 | 378 | 500 | 388 | 420 | 377 | 376 | 592 | 755 | 809 | 452 | 335 | 248 | 400 | 428 | 385 | 404 | 629 | 645 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.4 | 5.5 | 5.3 | 5.5 | 4.5 | 4.7 | – | 4.3 | 4.1 | 3.8 | 4.4 | 4.9 | 5.1 | 5.2 | 6.7 | 7.1 | 6.2 | 4.5 | 4.6 | 5.1 |
Std Deviation (1e-4) | 1.0 | 0.8 | 0.8 | 1.0 | 1.3 | 1.1 | 1.0 | – | 0.9 | 1.0 | 0.8 | 1.1 | 0.9 | 1.2 | 1.2 | 1.2 | 1.6 | 1.2 | 1.0 | 1.3 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.0 | -0.28 | -0.23 | – | -0.36 | -0.42 | -0.55 | -0.32 | -0.18 | -0.1 | -0.07 | 0.29 | 0.38 | 0.18 | -0.29 | -0.26 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.5 | 1.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.6 | 1.2 | 3.6 | 2.3 | 2.9 |
Bud | 0 | 0.7 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.2 | 1.2 | -1.1 | 0.4 | 0 |
Bud Neck | -2.2 | -1.0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -3.7 | -4.6 | -2.5 | -2.1 | -3.0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.4 | -1.8 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.5 | 2.0 | -3.4 | -1.7 | -1.9 |
Cytoplasm | 0.7 | -1.7 | -0.7 | -2.0 | – | -6.8 | -7.5 | -10.1 | -8.4 | -2.8 | -2.2 | 0.3 | -3.8 | -2.6 | -2.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 2.5 | 3.1 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 2.7 | 2.1 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.5267 | 2.9713 | 2.6558 | 2.4211 | 2.1536 | 2.7935 | 0.0158 | 1.4581 | 1.2229 | 1.3613 | 0.952 | 0.7899 | 0.8092 | 1.0059 | 0.8444 | 1.0189 | 0.9905 | 0.4962 |
Actin | 0.0516 | 0.0082 | 0.03 | 0.0069 | 0.065 | 0.0251 | 0.0851 | 0.049 | 0.0676 | 0.1114 | 0.0476 | 0.0305 | 0.0356 | 0.0122 | 0.0353 | 0.0072 | 0.0331 | 0.0187 |
Bud | 0.0021 | 0.0012 | 0.002 | 0.0008 | 0.0043 | 0.0013 | 0.0009 | 0.0008 | 0.0085 | 0.0059 | 0.0159 | 0.001 | 0.0105 | 0.0123 | 0.015 | 0.0249 | 0.0139 | 0.024 |
Bud Neck | 0.0162 | 0.0053 | 0.0254 | 0.0204 | 0.0288 | 0.0375 | 0.012 | 0.0091 | 0.0331 | 0.0174 | 0.0075 | 0.0624 | 0.0053 | 0.0044 | 0.0099 | 0.0076 | 0.0034 | 0.017 |
Bud Periphery | 0.0088 | 0.0058 | 0.0159 | 0.0086 | 0.0205 | 0.0117 | 0.0051 | 0.0029 | 0.0106 | 0.0146 | 0.0221 | 0.0114 | 0.0159 | 0.0056 | 0.0177 | 0.0272 | 0.0156 | 0.0295 |
Bud Site | 0.0349 | 0.129 | 0.0834 | 0.007 | 0.0184 | 0.0028 | 0.0286 | 0.1211 | 0.1158 | 0.0158 | 0.0082 | 0.005 | 0.0299 | 0.1316 | 0.074 | 0.0386 | 0.0131 | 0.0063 |
Cell Periphery | 0.1345 | 0.1342 | 0.1169 | 0.1754 | 0.1721 | 0.126 | 0.0937 | 0.1245 | 0.1272 | 0.0463 | 0.0212 | 0.162 | 0.0635 | 0.0707 | 0.0722 | 0.0478 | 0.053 | 0.1078 |
Cytoplasm | 0.3156 | 0.4798 | 0.4026 | 0.4726 | 0.4382 | 0.5311 | 0.3658 | 0.5229 | 0.3831 | 0.4737 | 0.3574 | 0.6106 | 0.2864 | 0.4981 | 0.4081 | 0.4687 | 0.4869 | 0.49 |
Cytoplasmic Foci | 0.0169 | 0.0036 | 0.0071 | 0.0041 | 0.0075 | 0.0071 | 0.0349 | 0.0085 | 0.0194 | 0.0483 | 0.0128 | 0.0105 | 0.0416 | 0.0115 | 0.0178 | 0.026 | 0.0116 | 0.0146 |
Eisosomes | 0.0085 | 0.0074 | 0.0106 | 0.0035 | 0.0043 | 0.005 | 0.0121 | 0.0184 | 0.0074 | 0.0027 | 0.0024 | 0.0029 | 0.0057 | 0.0042 | 0.0058 | 0.0018 | 0.001 | 0.0031 |
Endoplasmic Reticulum | 0.0075 | 0.0044 | 0.0051 | 0.0058 | 0.0021 | 0.0039 | 0.007 | 0.0046 | 0.0044 | 0.007 | 0.0052 | 0.0077 | 0.0136 | 0.0072 | 0.0049 | 0.0113 | 0.0032 | 0.0045 |
Endosome | 0.0246 | 0.0006 | 0.001 | 0.0005 | 0.0069 | 0.0021 | 0.0177 | 0.001 | 0.0099 | 0.0224 | 0.0241 | 0.002 | 0.0269 | 0.0027 | 0.0114 | 0.0358 | 0.0129 | 0.0036 |
Golgi | 0.0062 | 0.0009 | 0.0023 | 0.0006 | 0.0081 | 0.0032 | 0.0123 | 0.0029 | 0.0064 | 0.0056 | 0.0046 | 0.0017 | 0.0118 | 0.0045 | 0.0085 | 0.0127 | 0.0124 | 0.0033 |
Lipid Particles | 0.0088 | 0.001 | 0.0027 | 0.0015 | 0.0048 | 0.0053 | 0.0277 | 0.0019 | 0.017 | 0.0037 | 0.0071 | 0.0023 | 0.0199 | 0.0035 | 0.0043 | 0.0089 | 0.0162 | 0.0053 |
Mitochondria | 0.0076 | 0.0004 | 0.0062 | 0.0005 | 0.0284 | 0.0029 | 0.0296 | 0.0088 | 0.0069 | 0.0134 | 0.0082 | 0.0003 | 0.026 | 0.0012 | 0.0059 | 0.0108 | 0.0032 | 0.0011 |
None | 0.3161 | 0.2144 | 0.2739 | 0.2816 | 0.1745 | 0.2292 | 0.2265 | 0.1194 | 0.1631 | 0.1832 | 0.3951 | 0.0858 | 0.3304 | 0.219 | 0.2964 | 0.2298 | 0.3038 | 0.2384 |
Nuclear Periphery | 0.0137 | 0.0003 | 0.0002 | 0.0006 | 0.0013 | 0.0003 | 0.0022 | 0.0003 | 0.0018 | 0.0076 | 0.0279 | 0.0002 | 0.0151 | 0.0005 | 0.001 | 0.004 | 0.0016 | 0.0009 |
Nucleolus | 0.0029 | 0.0004 | 0.0004 | 0.0009 | 0.0009 | 0.0004 | 0.0031 | 0.0003 | 0.0028 | 0.0018 | 0.0039 | 0.0004 | 0.0062 | 0.0007 | 0.0013 | 0.001 | 0.0014 | 0.0009 |
Nucleus | 0.0047 | 0.0008 | 0.0006 | 0.001 | 0.0011 | 0.0006 | 0.0032 | 0.0006 | 0.0029 | 0.0046 | 0.0111 | 0.0006 | 0.0164 | 0.0053 | 0.0024 | 0.0031 | 0.0026 | 0.0063 |
Peroxisomes | 0.0064 | 0.0002 | 0.0118 | 0.0004 | 0.007 | 0.0014 | 0.0191 | 0.0011 | 0.0057 | 0.0077 | 0.0016 | 0.0002 | 0.0085 | 0.0009 | 0.0025 | 0.0046 | 0.0049 | 0.0131 |
Punctate Nuclear | 0.0045 | 0.0003 | 0.0005 | 0.0047 | 0.0039 | 0.0011 | 0.0098 | 0.0004 | 0.0027 | 0.004 | 0.0054 | 0.0006 | 0.019 | 0.0021 | 0.0017 | 0.0224 | 0.0032 | 0.004 |
Vacuole | 0.0059 | 0.0016 | 0.0013 | 0.0025 | 0.0015 | 0.0019 | 0.0028 | 0.0013 | 0.0031 | 0.0023 | 0.0061 | 0.0017 | 0.008 | 0.0017 | 0.0035 | 0.0044 | 0.0024 | 0.0072 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0008 | 0.0001 | 0.0005 | 0.0008 | 0.0048 | 0.0001 | 0.004 | 0.0001 | 0.0005 | 0.0014 | 0.0005 | 0.0005 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.2329 | 5.8322 | 8.6555 | 11.3748 | 10.3093 | 9.7809 | 11.9782 | 12.562 | 12.0109 | 13.3757 |
Translational Efficiency | 0.5525 | 0.9438 | 0.6767 | 0.5682 | 0.4639 | 0.6253 | 0.6236 | 0.5021 | 0.6074 | 0.5497 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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