Standard name
Human Ortholog
Description Kinesin-related motor protein involved in mitotic spindle positioning; stabilizes microtubules by targeting Bik1p to the plus end; functions as a microtubule polymerase and catastrophe inhibitor in vitro; Kip2p levels are controlled during the cell cycle
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.06 0.24 0.15 0.14 0 0.12 0.08 0 – 0 0.08 0.05 0.05 0 0.22 0.25 0.24 0.08 0.09 0 0.1 0.13 0.15
Bud 0.09 0.07 0.08 0.06 0.08 0.11 0.14 0.1 0.1 – 0.1 0.15 0.05 0 0 0.05 0.06 0 0.05 0.06 0 0.15 0.13 0.15
Bud Neck 0 0 0.12 0 0.07 0.06 0 0.07 0 – 0 0.08 0 0 0 0 0 0 0.06 0.12 0.06 0.21 0.07 0.09
Bud Site 0 0 0.05 0.07 0 0.05 0 0 0.06 – 0.05 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.16 0.27 0.18 0.31 0.41 0.2 0.24 0.25 0.16 – 0.15 0.13 0.13 0.3 0.27 0.09 0.12 0 0.05 0.1 0.07 0.1 0.12 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0.23 0.16 0 0 0 0.06 0 0 0 – 0 0 0.19 0.33 0.31 0.2 0.12 0.19 0.2 0.18 0.16 0 0.09 0.07
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.19 0.19 0.29 0 0 0 0 0 0
Mitochondria 0.26 0.36 0 0.18 0.09 0.38 0.39 0.28 0.72 – 0.68 0.47 0.4 0.06 0.11 0.08 0.19 0.05 0.09 0.14 0.2 0.16 0.16 0.14
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Peroxisomes 0.1 0.06 0 0 0 0 0 0 0 – 0 0 0.12 0.21 0.24 0.06 0 0.1 0.05 0 0 0 0.09 0.06
SpindlePole 0.23 0.08 0.53 0.32 0.34 0.29 0.29 0.4 0.17 – 0.17 0.34 0.11 0.18 0.16 0.26 0.12 0.29 0.29 0.17 0.3 0.09 0.14 0.15
Vac/Vac Membrane 0.06 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0.12 0.1 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 14 14 55 34 28 10 41 26 9 – 6 18 4 6 1 19 4 5 19 31 13 4 16 25
Bud 20 16 19 15 16 30 47 33 27 – 28 35 4 4 6 4 1 0 11 19 6 6 16 26
Bud Neck 4 1 28 10 14 18 15 23 9 – 6 18 0 0 0 0 0 0 16 41 21 8 9 15
Bud Site 5 3 12 16 4 14 13 9 16 – 13 16 3 1 2 1 0 0 – – – – – –
Cell Periphery 0 0 2 1 1 1 1 0 0 – 1 1 0 1 0 1 1 0 0 0 0 0 0 0
Cytoplasm 33 62 42 72 84 57 84 82 43 – 41 31 10 39 37 8 2 0 13 32 23 4 14 19
Endoplasmic Reticulum 3 8 6 7 4 6 4 3 8 – 1 11 2 4 3 4 0 1 0 0 0 0 1 2
Endosome 48 37 5 6 3 16 9 7 2 – 2 3 14 43 42 17 2 4 52 60 53 1 11 12
Golgi 0 1 1 1 0 0 0 0 0 – 0 0 0 0 0 16 3 6 1 1 2 0 2 0
Mitochondria 54 83 8 43 18 107 135 92 198 – 185 110 30 8 15 7 3 1 24 46 68 6 19 23
Nucleus 2 2 7 5 1 7 12 5 3 – 7 12 0 2 1 1 0 0 0 2 0 0 0 0
Nuclear Periphery 1 1 0 1 0 2 0 0 0 – 2 1 1 0 0 1 0 0 1 1 0 0 0 0
Nucleolus 8 3 0 0 1 5 8 3 3 – 3 4 3 0 3 2 1 0 1 4 12 0 0 0
Peroxisomes 21 15 2 8 4 9 4 6 5 – 4 3 9 28 32 5 0 2 11 10 11 1 10 9
SpindlePole 49 19 124 74 71 80 99 134 47 – 45 78 8 24 22 22 2 6 75 58 103 3 17 26
Vac/Vac Membrane 12 11 6 1 2 4 4 3 4 – 3 2 3 2 1 6 2 2 7 14 7 1 1 1
Unique Cell Count 211 232 234 233 207 279 345 334 275 272 232 75 132 135 85 16 21 260 339 347 41 124 171
Labelled Cell Count 274 276 317 294 251 366 476 426 374 347 343 91 162 165 114 21 27 260 339 347 41 124 171


Microtubule, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.4 5.5 5.0 5.5 4.3 4.4 4.7 3.9 – 3.6 4.1 5.0 5.5 5.5 9.1 8.9 9.1 – 5.0 5.1
Std Deviation (1e-4) 0.7 0.8 0.6 0.8 0.9 1.0 0.7 1.0 1.0 – 0.9 0.7 0.7 1.0 1.3 1.2 1.1 0.9 – 1.7 1.5
Intensity Change (Log2) – – – -0.15 -0.01 -0.35 -0.32 -0.24 -0.51 – -0.62 -0.44 -0.14 -0.01 0.01 0.71 0.7 0.72 – -0.15 -0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.5 -2.7 0 0 0 0 – 0 0 -3.5 -4.7 -5.9 -0.2 0 0
Bud -0.7 -0.2 0 0 0 0 – 0 0 -0.8 -1.9 -1.4 -1.0 0 0
Bud Neck -3.0 -1.9 0 0 0 0 – 0 0 -3.1 -4.1 -4.2 -3.3 0 0
Bud Site 0.8 -1.8 0 0 0 0 – 0 0 0 0 -1.8 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 3.3 5.3 0.7 1.8 1.9 -0.7 – -0.9 -1.4 -0.9 2.6 2.1 -1.9 0 0
Endoplasmic Reticulum 0.3 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0.3 0 0 0 0 0 – 0 0 0 8.3 8.0 5.6 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 5.2 2.3 0 0 0 0 – 0 0 8.4 1.2 2.9 0 0 0
Nucleus -0.6 0 -0.3 0.3 0 0 – -0.3 1.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 6.8 7.3 0 0 0
SpindlePole -4.6 -3.9 0 0 0 0 – 0 0 -6.4 -6.5 -6.9 -4.3 -3.1 -2.1
Vacuole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.7596 -0.2324 -0.3933 -0.0445 -0.2381 -0.2944 0.1481 0.8703 0.8587 1.1295 1.0292 0.8487 -0.21 0.0467 0.1416 0.4022 0.4114 0.1336
Actin 0.0356 0.0181 0.0303 0.0175 0.0264 0.0177 0.04 0.0348 0.0322 0.0158 0.0344 0.0135 0.014 0.0246 0.0231 0.0678 0.0033 0.0186
Bud 0.0008 0.0026 0.002 0.0058 0.0015 0.0009 0.0009 0.0061 0.0038 0.0012 0.0018 0.0009 0.0005 0.0023 0.0023 0.0008 0.0017 0.0006
Bud Neck 0.0151 0.0035 0.0426 0.0623 0.0032 0.0389 0.0062 0.006 0.0797 0.12 0.0078 0.0342 0.0075 0.0106 0.0833 0.0573 0.0008 0.0293
Bud Periphery 0.0009 0.0029 0.0031 0.0042 0.0016 0.0014 0.0016 0.0053 0.0047 0.0012 0.0026 0.001 0.0006 0.0023 0.0032 0.0022 0.0014 0.0019
Bud Site 0.0164 0.0645 0.0339 0.0085 0.0027 0.0029 0.0116 0.1042 0.0462 0.0114 0.0042 0.004 0.0212 0.0842 0.0165 0.0017 0.0016 0.0013
Cell Periphery 0.0005 0.0006 0.0006 0.0002 0.0002 0.0003 0.0004 0.0009 0.0004 0.0001 0.0003 0.0001 0.0008 0.0005 0.001 0.0005 0.0001 0.0002
Cytoplasm 0.0342 0.1917 0.1179 0.1159 0.0136 0.0411 0.0262 0.1014 0.1066 0.0606 0.0353 0.0404 0.0354 0.1576 0.1087 0.0119 0.033 0.0348
Cytoplasmic Foci 0.323 0.2704 0.3311 0.3646 0.3109 0.3478 0.3511 0.2736 0.3249 0.3814 0.2823 0.3046 0.3372 0.2567 0.37 0.3556 0.3633 0.3513
Eisosomes 0.0008 0.0004 0.0004 0.0001 0.0003 0.0002 0.0003 0.0003 0.0003 0.0001 0.0002 0.0001 0.0002 0.0003 0.0011 0.0006 0 0.0003
Endoplasmic Reticulum 0.0066 0.0032 0.0033 0.0042 0.0024 0.0027 0.0015 0.0027 0.0026 0.0016 0.0023 0.0007 0.0039 0.0034 0.0033 0.0011 0.0005 0.0009
Endosome 0.1341 0.0804 0.1075 0.0994 0.146 0.086 0.1132 0.1065 0.0835 0.0739 0.1506 0.0833 0.1375 0.0702 0.08 0.0923 0.1938 0.0556
Golgi 0.0287 0.0157 0.0219 0.03 0.0488 0.0315 0.0357 0.027 0.0248 0.0245 0.066 0.0349 0.026 0.0132 0.0212 0.0222 0.0839 0.0239
Lipid Particles 0.1013 0.0323 0.0259 0.0186 0.0702 0.0671 0.0674 0.0136 0.0197 0.0229 0.0199 0.0559 0.0894 0.0416 0.0238 0.0089 0.0262 0.0793
Mitochondria 0.0103 0.0166 0.0238 0.0067 0.0148 0.034 0.0241 0.0065 0.0157 0.0132 0.0323 0.0266 0.0177 0.0118 0.0124 0.054 0.0132 0.0246
None 0.1123 0.1581 0.1343 0.1131 0.0327 0.0376 0.0635 0.0589 0.0778 0.0394 0.0154 0.0183 0.0675 0.2104 0.1367 0.1122 0.0126 0.0539
Nuclear Periphery 0.0115 0.0018 0.002 0.0023 0.002 0.0029 0.0049 0.0043 0.0029 0.0009 0.0031 0.0009 0.0153 0.0009 0.0017 0.0009 0.0004 0.0003
Nucleolus 0.0037 0.0017 0.001 0.0004 0.0005 0.0015 0.0047 0.0015 0.0012 0.0005 0.0003 0.0006 0.0016 0.0007 0.001 0.0007 0.0005 0.0008
Nucleus 0.0021 0.0057 0.0023 0.0012 0.0007 0.0023 0.006 0.0019 0.0016 0.0006 0.0011 0.0008 0.0062 0.0013 0.0017 0.0006 0.0005 0.0005
Peroxisomes 0.1406 0.1158 0.0895 0.1335 0.3132 0.2632 0.2162 0.2236 0.155 0.2183 0.3301 0.3663 0.1885 0.0934 0.0928 0.1577 0.2507 0.3041
Punctate Nuclear 0.0133 0.0062 0.0165 0.0073 0.0054 0.0132 0.0182 0.0158 0.0121 0.0084 0.0035 0.0102 0.0122 0.01 0.0062 0.0454 0.0015 0.0157
Vacuole 0.0063 0.0067 0.0082 0.0033 0.0017 0.0051 0.0049 0.0042 0.0034 0.0028 0.0041 0.0018 0.0147 0.0035 0.0083 0.0043 0.0092 0.0015
Vacuole Periphery 0.0021 0.001 0.0018 0.0009 0.001 0.0014 0.0015 0.001 0.001 0.0012 0.0025 0.0009 0.0021 0.0005 0.0015 0.0014 0.0018 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.0231 19.5322 12.7683 13.4138 13.1527 8.319 21.6298 23.9609 16.5661 12.9163
Translational Efficiency 0.9409 0.7318 0.9388 0.7606 0.5722 0.7516 0.7609 0.6075 0.7492 0.6603

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Kinesin-related motor protein involved in mitotic spindle positioning; stabilizes microtubules by targeting Bik1p to the plus end; functions as a microtubule polymerase and catastrophe inhibitor in vitro; Kip2p levels are controlled during the cell cycle
Localization
Cell Percentages mitochondrion (17%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Kip2

Kip2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Kip2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available