Standard name | |
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Human Ortholog | |
Description | Protein that regulates Cdc42p and Rho1p; functions in the late steps of cytokinesis and cell separation; sustains Rho1p at the cell division site after actomyosin ring contraction; inhibits the activation of Cdc42-Cla4 at the cell division site to prevent budding inside the old bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 23 | 69 | 48 | 27 | 41 | 25 | 36 | – | 20 | 6 | 6 | 11 | 33 | 6 | 0 | 21 | 22 | 12 | 24 | 39 | 24 | 36 | 74 | 43 |
Bud | 12 | 23 | 16 | 4 | 18 | 22 | 30 | – | 23 | 18 | 18 | 15 | 5 | 2 | 2 | 8 | 5 | 6 | 24 | 55 | 28 | 51 | 20 | 15 |
Bud Neck | 8 | 10 | 26 | 21 | 20 | 19 | 28 | – | 10 | 13 | 7 | 9 | 0 | 1 | 1 | 7 | 11 | 7 | 45 | 97 | 87 | 78 | 42 | 18 |
Bud Site | 19 | 56 | 36 | 13 | 27 | 48 | 18 | – | 13 | 2 | 8 | 8 | 34 | 3 | 0 | 11 | 12 | 12 | – | – | – | – | – | – |
Cell Periphery | 3 | 6 | 8 | 3 | 3 | 8 | 5 | – | 6 | 2 | 5 | 3 | 1 | 9 | 8 | 12 | 6 | 7 | 7 | 8 | 15 | 13 | 30 | 30 |
Cytoplasm | 70 | 114 | 139 | 81 | 155 | 113 | 225 | – | 187 | 183 | 141 | 189 | 259 | 312 | 446 | 156 | 109 | 118 | 32 | 45 | 53 | 38 | 91 | 159 |
Endoplasmic Reticulum | 1 | 4 | 2 | 1 | 0 | 5 | 3 | – | 3 | 0 | 1 | 0 | 4 | 4 | 3 | 13 | 8 | 10 | 0 | 2 | 2 | 4 | 6 | 5 |
Endosome | 1 | 4 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 4 | 0 | 2 | 1 | 1 | 6 | 3 | 5 | 5 | 3 | 4 | 1 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 0 | 3 | 1 | 1 | 3 | 0 |
Mitochondria | 6 | 8 | 0 | 5 | 2 | 24 | 42 | – | 104 | 103 | 118 | 75 | 7 | 2 | 3 | 8 | 3 | 0 | 9 | 13 | 20 | 22 | 30 | 39 |
Nucleus | 0 | 2 | 3 | 2 | 4 | 1 | 6 | – | 0 | 3 | 1 | 2 | 0 | 0 | 1 | 3 | 5 | 3 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 1 | 1 | 0 | 2 | 1 | 0 | 0 | – | 1 | 2 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 10 | 18 | 0 | 2 | 3 | 24 | 4 | – | 6 | 1 | 2 | 3 | 24 | 0 | 0 | 3 | 4 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 7 | 28 | 45 | 30 | 53 | 44 | 42 | – | 12 | 6 | 9 | 17 | 15 | 7 | 0 | 22 | 25 | 32 | 2 | 1 | 7 | 4 | 1 | 3 |
Vac/Vac Membrane | 1 | 1 | 1 | 1 | 0 | 1 | 2 | – | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 6 | 2 | 8 | 7 | 9 | 7 | 13 |
Unique Cell Count | 128 | 245 | 231 | 147 | 248 | 237 | 348 | 312 | 269 | 239 | 272 | 332 | 332 | 456 | 213 | 166 | 185 | 155 | 284 | 261 | 268 | 322 | 337 | |
Labelled Cell Count | 162 | 345 | 324 | 192 | 328 | 334 | 441 | 387 | 341 | 316 | 335 | 388 | 347 | 468 | 266 | 214 | 231 | 155 | 284 | 261 | 268 | 322 | 337 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 5.1 | 5.4 | 4.7 | 5.5 | 4.4 | 4.4 | – | 3.8 | 3.3 | 3.1 | 3.6 | 4.5 | 4.4 | 4.3 | 7.2 | 7.4 | 7.4 | 4.5 | 4.3 | 4.2 |
Std Deviation (1e-4) | 1.1 | 1.2 | 1.3 | 1.1 | 2.0 | 1.3 | 1.3 | – | 1.8 | 1.1 | 1.0 | 1.2 | 1.0 | 1.2 | 1.1 | 1.8 | 1.8 | 2.2 | 1.2 | 1.6 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.2 | 0.04 | -0.3 | -0.3 | – | -0.52 | -0.69 | -0.78 | -0.57 | -0.27 | -0.3 | -0.33 | 0.42 | 0.45 | 0.47 | -0.27 | -0.34 | -0.35 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | -1.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -1.9 | -4.1 |
Bud | 0 | 0.1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.5 | -1.7 | -1.7 |
Bud Neck | 0 | -1.2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.2 | -1.6 | -2.8 |
Bud Site | 0 | -1.5 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.6 | -2.5 | -2.9 |
Cell Periphery | 0 | -1.6 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0.1 | 0.2 |
Cytoplasm | -1.0 | 0.5 | -2.7 | 1.1 | – | -0.1 | 1.8 | -0.3 | 2.2 | 4.6 | 9.9 | 13.1 | 2.9 | 1.1 | 0.8 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 2.7 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0.5 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.7 | -1.1 | -0.6 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2112 | -0.3316 | -0.0267 | -0.602 | -0.4024 | -0.0207 | 1.5781 | 0.0329 | -0.0543 | 0.0049 | -0.3245 | 0.434 | 1.2818 | -0.362 | -0.6433 | -0.5548 | -0.3544 | -0.2526 |
Actin | 0.045 | 0.0099 | 0.0666 | 0.0699 | 0.0329 | 0.0117 | 0.0804 | 0.0093 | 0.0363 | 0.0055 | 0.0165 | 0.0105 | 0.1518 | 0.0035 | 0.0377 | 0.0155 | 0.0965 | 0.0095 |
Bud | 0.0031 | 0.0106 | 0.004 | 0.0248 | 0.0033 | 0.001 | 0.0021 | 0.0069 | 0.0029 | 0.0032 | 0.0051 | 0.0005 | 0.0018 | 0.0025 | 0.001 | 0.0002 | 0.0005 | 0.0003 |
Bud Neck | 0.1175 | 0.0083 | 0.0999 | 0.1646 | 0.0555 | 0.5566 | 0.0844 | 0.0196 | 0.1234 | 0.2732 | 0.1104 | 0.6864 | 0.1111 | 0.0043 | 0.2111 | 0.2692 | 0.0823 | 0.6834 |
Bud Periphery | 0.0055 | 0.0125 | 0.0078 | 0.0043 | 0.0157 | 0.0063 | 0.0042 | 0.0139 | 0.0035 | 0.01 | 0.021 | 0.0015 | 0.0026 | 0.0047 | 0.0012 | 0.0002 | 0.0008 | 0.0013 |
Bud Site | 0.1423 | 0.2389 | 0.1137 | 0.0293 | 0.0053 | 0.0047 | 0.1514 | 0.3429 | 0.1283 | 0.0251 | 0.0032 | 0.0098 | 0.1774 | 0.217 | 0.0715 | 0.0009 | 0.0006 | 0.0006 |
Cell Periphery | 0.0168 | 0.0059 | 0.0038 | 0.001 | 0.0024 | 0.0029 | 0.0198 | 0.012 | 0.0036 | 0.0017 | 0.0018 | 0.0029 | 0.0163 | 0.0065 | 0.0032 | 0.0003 | 0.0001 | 0.0032 |
Cytoplasm | 0.1108 | 0.1488 | 0.0937 | 0.0569 | 0.0915 | 0.1354 | 0.2031 | 0.1861 | 0.133 | 0.0675 | 0.1398 | 0.1402 | 0.2019 | 0.2173 | 0.1794 | 0.0318 | 0.0657 | 0.1313 |
Cytoplasmic Foci | 0.0598 | 0.0068 | 0.0445 | 0.033 | 0.044 | 0.009 | 0.0539 | 0.0105 | 0.0238 | 0.0239 | 0.0772 | 0.0206 | 0.0521 | 0.0093 | 0.0098 | 0.0792 | 0.021 | 0.0081 |
Eisosomes | 0.0014 | 0.001 | 0.0013 | 0.0005 | 0.0039 | 0.0005 | 0.001 | 0.0009 | 0.0007 | 0.0004 | 0.0009 | 0.0002 | 0.0023 | 0.0006 | 0.001 | 0.0005 | 0.0005 | 0.0004 |
Endoplasmic Reticulum | 0.0051 | 0.0025 | 0.0044 | 0.0025 | 0.0056 | 0.002 | 0.0149 | 0.0039 | 0.0022 | 0.001 | 0.0024 | 0.0009 | 0.0121 | 0.0027 | 0.0028 | 0.0016 | 0.0044 | 0.0022 |
Endosome | 0.0348 | 0.0024 | 0.0315 | 0.0184 | 0.0576 | 0.0018 | 0.0561 | 0.0048 | 0.0072 | 0.0551 | 0.0049 | 0.0033 | 0.0276 | 0.0005 | 0.013 | 0.0183 | 0.0282 | 0.0043 |
Golgi | 0.0152 | 0.0005 | 0.0113 | 0.0278 | 0.0262 | 0.0014 | 0.0175 | 0.0007 | 0.0073 | 0.0285 | 0.0031 | 0.0025 | 0.0143 | 0.0002 | 0.0062 | 0.0054 | 0.0169 | 0.0016 |
Lipid Particles | 0.0548 | 0.0006 | 0.0071 | 0.0371 | 0.0211 | 0.0017 | 0.0131 | 0.0011 | 0.004 | 0.0568 | 0.0015 | 0.0054 | 0.0074 | 0.0006 | 0.0014 | 0.0136 | 0.0282 | 0.0015 |
Mitochondria | 0.0073 | 0.0008 | 0.0372 | 0.0091 | 0.0328 | 0.0133 | 0.0109 | 0.0022 | 0.0203 | 0.0522 | 0.0161 | 0.001 | 0.0149 | 0.0002 | 0.0019 | 0.0014 | 0.005 | 0.0011 |
None | 0.2639 | 0.5403 | 0.4453 | 0.4814 | 0.5356 | 0.2318 | 0.1802 | 0.3725 | 0.4863 | 0.3549 | 0.5784 | 0.109 | 0.1637 | 0.5257 | 0.4431 | 0.5281 | 0.5753 | 0.1483 |
Nuclear Periphery | 0.02 | 0.001 | 0.0034 | 0.0006 | 0.0069 | 0.0075 | 0.0398 | 0.0013 | 0.0007 | 0.0005 | 0.0009 | 0.0003 | 0.0099 | 0.0004 | 0.0032 | 0.0054 | 0.0162 | 0.0004 |
Nucleolus | 0.0048 | 0.0008 | 0.002 | 0.001 | 0.0044 | 0.0006 | 0.0031 | 0.0008 | 0.0011 | 0.0008 | 0.0007 | 0.0002 | 0.0022 | 0.0005 | 0.0006 | 0.0017 | 0.0004 | 0.0002 |
Nucleus | 0.0234 | 0.0029 | 0.0028 | 0.0033 | 0.0156 | 0.0086 | 0.0108 | 0.0035 | 0.0025 | 0.0013 | 0.0016 | 0.0006 | 0.0054 | 0.001 | 0.0039 | 0.0019 | 0.0021 | 0.0004 |
Peroxisomes | 0.024 | 0.0015 | 0.0066 | 0.0277 | 0.0097 | 0.0006 | 0.0325 | 0.0008 | 0.0083 | 0.0309 | 0.0017 | 0.0025 | 0.0064 | 0.0001 | 0.0015 | 0.0043 | 0.0474 | 0.0002 |
Punctate Nuclear | 0.0363 | 0.0019 | 0.0049 | 0.0041 | 0.0191 | 0.0011 | 0.0062 | 0.0026 | 0.0024 | 0.0013 | 0.0097 | 0.0012 | 0.0121 | 0.0015 | 0.0054 | 0.0194 | 0.0053 | 0.0006 |
Vacuole | 0.0058 | 0.0018 | 0.0058 | 0.002 | 0.0078 | 0.001 | 0.0103 | 0.0032 | 0.002 | 0.0048 | 0.0024 | 0.0004 | 0.0049 | 0.0009 | 0.0008 | 0.0006 | 0.0012 | 0.0011 |
Vacuole Periphery | 0.0022 | 0.0002 | 0.0022 | 0.0006 | 0.003 | 0.0006 | 0.0044 | 0.0003 | 0.0002 | 0.0015 | 0.0008 | 0.0001 | 0.0017 | 0.0001 | 0.0003 | 0.0004 | 0.0013 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.5727 | 9.097 | 41.4666 | 113.781 | 24.1891 | 4.3589 | 5.256 | 10.4945 | 66.8939 | 38.0005 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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