Standard name
Human Ortholog
Description Protein that regulates Cdc42p and Rho1p; functions in the late steps of cytokinesis and cell separation; sustains Rho1p at the cell division site after actomyosin ring contraction; inhibits the activation of Cdc42-Cla4 at the cell division site to prevent budding inside the old bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.18 0.28 0.21 0.18 0.17 0.11 0.1 0.06 0 0 0 0.1 0 0 0.1 0.13 0.06 0.16 0.14 0.1 0.13 0.23 0.13
Bud 0.09 0.09 0.07 0 0.07 0.09 0.09 0.07 0.07 0.08 0.06 0 0 0 0 0 0 0.16 0.2 0.11 0.19 0.06 0.05
Bud Neck 0.06 0 0.11 0.14 0.08 0.08 0.08 0 0.05 0 0 0 0 0 0 0.07 0 0.29 0.34 0.34 0.29 0.13 0.06
Bud Site 0.15 0.23 0.16 0.09 0.11 0.2 0.05 0 0 0 0 0.1 0 0 0.05 0.07 0.06
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0.06 0.05 0.09 0.09
Cytoplasm 0.55 0.47 0.6 0.55 0.62 0.48 0.65 0.6 0.68 0.59 0.69 0.78 0.94 0.98 0.73 0.66 0.64 0.21 0.16 0.2 0.14 0.28 0.47
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0.1 0.12 0.33 0.38 0.49 0.28 0 0 0 0 0 0 0.06 0.05 0.08 0.09 0.09 0.12
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.08 0.07 0 0 0 0.1 0 0 0 0 0 0.07 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.05 0.11 0.19 0.2 0.21 0.19 0.12 0 0 0 0.06 0.05 0 0 0.1 0.15 0.17 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 23 69 48 27 41 25 36 20 6 6 11 33 6 0 21 22 12 24 39 24 36 74 43
Bud 12 23 16 4 18 22 30 23 18 18 15 5 2 2 8 5 6 24 55 28 51 20 15
Bud Neck 8 10 26 21 20 19 28 10 13 7 9 0 1 1 7 11 7 45 97 87 78 42 18
Bud Site 19 56 36 13 27 48 18 13 2 8 8 34 3 0 11 12 12
Cell Periphery 3 6 8 3 3 8 5 6 2 5 3 1 9 8 12 6 7 7 8 15 13 30 30
Cytoplasm 70 114 139 81 155 113 225 187 183 141 189 259 312 446 156 109 118 32 45 53 38 91 159
Endoplasmic Reticulum 1 4 2 1 0 5 3 3 0 1 0 4 4 3 13 8 10 0 2 2 4 6 5
Endosome 1 4 0 0 0 0 0 0 0 0 1 4 0 2 1 1 6 3 5 5 3 4 1
Golgi 0 1 0 0 1 0 0 0 0 0 0 0 1 0 1 0 2 0 3 1 1 3 0
Mitochondria 6 8 0 5 2 24 42 104 103 118 75 7 2 3 8 3 0 9 13 20 22 30 39
Nucleus 0 2 3 2 4 1 6 0 3 1 2 0 0 1 3 5 3 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 1 1 0 2 1 0 0 1 2 0 2 2 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 10 18 0 2 3 24 4 6 1 2 3 24 0 0 3 4 9 0 0 0 0 0 0
SpindlePole 7 28 45 30 53 44 42 12 6 9 17 15 7 0 22 25 32 2 1 7 4 1 3
Vac/Vac Membrane 1 1 1 1 0 1 2 1 1 0 0 0 0 2 0 3 6 2 8 7 9 7 13
Unique Cell Count 128 245 231 147 248 237 348 312 269 239 272 332 332 456 213 166 185 155 284 261 268 322 337
Labelled Cell Count 162 345 324 192 328 334 441 387 341 316 335 388 347 468 266 214 231 155 284 261 268 322 337


Bud Neck

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.1 5.4 4.7 5.5 4.4 4.4 3.8 3.3 3.1 3.6 4.5 4.4 4.3 7.2 7.4 7.4 4.5 4.3 4.2
Std Deviation (1e-4) 1.1 1.2 1.3 1.1 2.0 1.3 1.3 1.8 1.1 1.0 1.2 1.0 1.2 1.1 1.8 1.8 2.2 1.2 1.6 1.5
Intensity Change (Log2) -0.2 0.04 -0.3 -0.3 -0.52 -0.69 -0.78 -0.57 -0.27 -0.3 -0.33 0.42 0.45 0.47 -0.27 -0.34 -0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -1.2 0 0 0 0 0 0 0 0 0 -3.2 -1.9 -4.1
Bud 0 0.1 0 0 0 0 0 0 0 0 0 -1.5 -1.7 -1.7
Bud Neck 0 -1.2 0 0 0 0 0 0 0 0 0 -3.2 -1.6 -2.8
Bud Site 0 -1.5 0 0 0 0 0 0 0 0 0 -3.6 -2.5 -2.9
Cell Periphery 0 -1.6 0 0 0 0 0 0 0 0 0 1.1 0.1 0.2
Cytoplasm -1.0 0.5 -2.7 1.1 -0.1 1.8 -0.3 2.2 4.6 9.9 13.1 2.9 1.1 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 3.1 0 2.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0.5 0 0 0 0 0 0 0 0 0 -2.7 -1.1 -0.6
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2112 -0.3316 -0.0267 -0.602 -0.4024 -0.0207 1.5781 0.0329 -0.0543 0.0049 -0.3245 0.434 1.2818 -0.362 -0.6433 -0.5548 -0.3544 -0.2526
Actin 0.045 0.0099 0.0666 0.0699 0.0329 0.0117 0.0804 0.0093 0.0363 0.0055 0.0165 0.0105 0.1518 0.0035 0.0377 0.0155 0.0965 0.0095
Bud 0.0031 0.0106 0.004 0.0248 0.0033 0.001 0.0021 0.0069 0.0029 0.0032 0.0051 0.0005 0.0018 0.0025 0.001 0.0002 0.0005 0.0003
Bud Neck 0.1175 0.0083 0.0999 0.1646 0.0555 0.5566 0.0844 0.0196 0.1234 0.2732 0.1104 0.6864 0.1111 0.0043 0.2111 0.2692 0.0823 0.6834
Bud Periphery 0.0055 0.0125 0.0078 0.0043 0.0157 0.0063 0.0042 0.0139 0.0035 0.01 0.021 0.0015 0.0026 0.0047 0.0012 0.0002 0.0008 0.0013
Bud Site 0.1423 0.2389 0.1137 0.0293 0.0053 0.0047 0.1514 0.3429 0.1283 0.0251 0.0032 0.0098 0.1774 0.217 0.0715 0.0009 0.0006 0.0006
Cell Periphery 0.0168 0.0059 0.0038 0.001 0.0024 0.0029 0.0198 0.012 0.0036 0.0017 0.0018 0.0029 0.0163 0.0065 0.0032 0.0003 0.0001 0.0032
Cytoplasm 0.1108 0.1488 0.0937 0.0569 0.0915 0.1354 0.2031 0.1861 0.133 0.0675 0.1398 0.1402 0.2019 0.2173 0.1794 0.0318 0.0657 0.1313
Cytoplasmic Foci 0.0598 0.0068 0.0445 0.033 0.044 0.009 0.0539 0.0105 0.0238 0.0239 0.0772 0.0206 0.0521 0.0093 0.0098 0.0792 0.021 0.0081
Eisosomes 0.0014 0.001 0.0013 0.0005 0.0039 0.0005 0.001 0.0009 0.0007 0.0004 0.0009 0.0002 0.0023 0.0006 0.001 0.0005 0.0005 0.0004
Endoplasmic Reticulum 0.0051 0.0025 0.0044 0.0025 0.0056 0.002 0.0149 0.0039 0.0022 0.001 0.0024 0.0009 0.0121 0.0027 0.0028 0.0016 0.0044 0.0022
Endosome 0.0348 0.0024 0.0315 0.0184 0.0576 0.0018 0.0561 0.0048 0.0072 0.0551 0.0049 0.0033 0.0276 0.0005 0.013 0.0183 0.0282 0.0043
Golgi 0.0152 0.0005 0.0113 0.0278 0.0262 0.0014 0.0175 0.0007 0.0073 0.0285 0.0031 0.0025 0.0143 0.0002 0.0062 0.0054 0.0169 0.0016
Lipid Particles 0.0548 0.0006 0.0071 0.0371 0.0211 0.0017 0.0131 0.0011 0.004 0.0568 0.0015 0.0054 0.0074 0.0006 0.0014 0.0136 0.0282 0.0015
Mitochondria 0.0073 0.0008 0.0372 0.0091 0.0328 0.0133 0.0109 0.0022 0.0203 0.0522 0.0161 0.001 0.0149 0.0002 0.0019 0.0014 0.005 0.0011
None 0.2639 0.5403 0.4453 0.4814 0.5356 0.2318 0.1802 0.3725 0.4863 0.3549 0.5784 0.109 0.1637 0.5257 0.4431 0.5281 0.5753 0.1483
Nuclear Periphery 0.02 0.001 0.0034 0.0006 0.0069 0.0075 0.0398 0.0013 0.0007 0.0005 0.0009 0.0003 0.0099 0.0004 0.0032 0.0054 0.0162 0.0004
Nucleolus 0.0048 0.0008 0.002 0.001 0.0044 0.0006 0.0031 0.0008 0.0011 0.0008 0.0007 0.0002 0.0022 0.0005 0.0006 0.0017 0.0004 0.0002
Nucleus 0.0234 0.0029 0.0028 0.0033 0.0156 0.0086 0.0108 0.0035 0.0025 0.0013 0.0016 0.0006 0.0054 0.001 0.0039 0.0019 0.0021 0.0004
Peroxisomes 0.024 0.0015 0.0066 0.0277 0.0097 0.0006 0.0325 0.0008 0.0083 0.0309 0.0017 0.0025 0.0064 0.0001 0.0015 0.0043 0.0474 0.0002
Punctate Nuclear 0.0363 0.0019 0.0049 0.0041 0.0191 0.0011 0.0062 0.0026 0.0024 0.0013 0.0097 0.0012 0.0121 0.0015 0.0054 0.0194 0.0053 0.0006
Vacuole 0.0058 0.0018 0.0058 0.002 0.0078 0.001 0.0103 0.0032 0.002 0.0048 0.0024 0.0004 0.0049 0.0009 0.0008 0.0006 0.0012 0.0011
Vacuole Periphery 0.0022 0.0002 0.0022 0.0006 0.003 0.0006 0.0044 0.0003 0.0002 0.0015 0.0008 0.0001 0.0017 0.0001 0.0003 0.0004 0.0013 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.5727 9.097 41.4666 113.781 24.1891 4.3589 5.256 10.4945 66.8939 38.0005
Translational Efficiency

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein that regulates Cdc42p and Rho1p; functions in the late steps of cytokinesis and cell separation; sustains Rho1p at the cell division site after actomyosin ring contraction; inhibits the activation of Cdc42-Cla4 at the cell division site to prevent budding inside the old bud neck; transcription is regulated by Swi5p; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from bud neck to cytoplasm upon DNA replication stress
Localization
Cell Percentages bud neck (21%), cytoplasm (16%), mixed (23%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Aim44

Aim44


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aim44-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available