ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0
Bud – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0 0 – 0.26 – 0.23 0.33 0.28 0.33 – 0 0 0 0.06 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 – 0.05 – 0.05 0 0.07 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 – 0 – 0 0 0 0 – 0 0.06 0.05 0 0 0 0.05 0 0 0.05 0.08 0.07
Golgi – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0.26 0.12 – 0.18 – 0.24 0.24 0.18 0.28 – 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleus – 0.13 0.08 0 – 0.07 – 0 0 0 0 – 0.09 0.06 0 0.16 0.14 0.09 0 0.08 0 0.08 0.07 0.07
Nuclear Periphery – 0 0.15 0.21 – 0.08 – 0.05 0.08 0.06 0 – 0 0.1 0.09 0.12 0.12 0.15 0.1 0 0 0 0 0
Nucleolus – 0 0 0.06 – 0 – 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.87 0.87 0.85 – 0.58 – 0.55 0.49 0.53 0.49 – 0.85 0.89 0.9 0.8 0.8 0.82 0.6 0.86 0.86 0.79 0.75 0.74
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 – 0 – 2 4 2 0 – 0 0 0 0 0 0 3 0 0 0 0 0
Bud – 0 0 0 – 2 – 4 6 6 4 – 0 0 1 0 0 0 0 0 0 1 3 2
Bud Neck – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 1 – 4 – 5 6 7 6 – 0 0 0 2 3 0 0 0 0 0 0 0
Cytoplasm – 7 1 10 – 133 – 132 152 194 189 – 9 5 12 8 6 7 0 1 0 1 1 1
Endoplasmic Reticulum – 0 0 1 – 25 – 31 19 48 26 – 0 0 3 6 2 3 0 0 0 0 3 4
Endosome – 1 1 11 – 4 – 7 2 13 2 – 7 15 13 1 0 2 2 2 7 10 29 20
Golgi – 2 1 0 – 0 – 0 1 2 0 – 0 1 1 3 3 4 0 0 0 1 3 1
Mitochondria – 4 20 39 – 93 – 141 110 123 160 – 5 3 2 3 2 2 2 1 4 2 4 6
Nucleus – 26 6 6 – 33 – 21 7 16 12 – 21 17 10 22 18 15 1 17 10 15 27 21
Nuclear Periphery – 2 12 71 – 39 – 28 35 42 16 – 10 26 24 17 16 26 4 2 12 6 11 8
Nucleolus – 5 1 19 – 2 – 6 3 5 4 – 8 15 10 4 3 6 1 1 2 1 6 5
Peroxisomes – 0 0 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 1 – 1 – 0 0 1 0 – 1 0 1 0 0 0 0 0 0 0 1 1
Vac/Vac Membrane – 170 68 283 – 291 – 318 226 364 283 – 193 239 251 111 102 137 28 178 262 164 286 222
Unique Cell Count 195 78 333 506 578 464 682 575 226 268 278 138 128 168 47 207 305 208 381 300
Labelled Cell Count 217 110 442 627 695 571 823 702 254 321 328 177 155 202 47 207 305 208 381 300


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 13.8 8.6 8.0 – 6.9 – 6.4 6.5 6.8 6.6 – 11.5 10.4 11.0 15.2 16.8 17.1 13.3 12.4 12.2
Std Deviation (1e-4) – 3.9 2.6 2.2 – 2.0 – 1.7 1.7 1.7 1.6 – 3.3 2.8 3.1 5.9 6.0 5.3 4.3 3.8 3.8
Intensity Change (Log2) – – – -0.09 – -0.32 – -0.41 -0.41 -0.33 -0.39 – 0.42 0.28 0.36 0.83 0.97 0.99 0.64 0.53 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Neck 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Bud Site 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cell Periphery 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0 – 4.9 – 4.4 5.7 5.2 5.8 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Endosome 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Golgi 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Mitochondria -3.2 – -1.5 – -0.2 -0.4 -1.6 0.4 – -6.5 -7.7 0 -5.4 -5.4 -6.3
Nucleus 0 – -0.4 – 0 0 0 0 – 0.4 -0.4 0 1.7 1.4 0.3
Nuclear Periphery 1.2 – -2.2 – 0 -2.3 -3.0 0 – -3.2 -1.4 -1.7 -0.6 -0.6 0
Nucleolus 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Peroxisomes 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0 – 0 – 0 0 0 0 – 0 0 0 0 0 0
Vacuole -0.5 – -5.0 – -5.4 -6.3 -5.7 -6.3 – -0.4 0.5 0.8 -1.3 -1.4 -1.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.465 7.6739 6.2347 6.1634 5.0129 6.0766 7.8345 9.8147 8.621 7.4616 7.5909 8.5835 5.6254 7.6276 6.2274 6.2453 5.536 5.9669
Actin 0.0094 0 0.0041 0.0163 0.0134 0.0051 0.0032 0 0 0 0 0 0.0005 0 0 0 0 0
Bud 0.0003 0.0002 0.0002 0.0001 0.001 0.0002 0.0003 0 0.0001 0 0 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0
Bud Neck 0.0014 0 0.0006 0.0002 0.0004 0.0006 0.0011 0 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001
Bud Periphery 0.0007 0.0004 0.0005 0.0002 0.0015 0.0006 0.001 0.0001 0.0003 0.0001 0 0.0003 0.0003 0.0002 0.0008 0.0006 0.0003 0.0002
Bud Site 0.0014 0.0002 0.0009 0.0002 0.0022 0.0005 0.0004 0 0.0001 0 0 0 0.0002 0.0001 0.0002 0 0 0
Cell Periphery 0.0015 0.001 0.0017 0.0007 0.0009 0.0008 0.0013 0.0006 0.0012 0.0004 0.0003 0.0005 0.0019 0.0013 0.0022 0.0006 0.0004 0.0007
Cytoplasm 0.0036 0.0015 0.0015 0.0002 0.003 0.0006 0.0005 0.0006 0.0006 0.0001 0 0.0001 0.0003 0.0011 0.0003 0.0001 0.0002 0.0001
Cytoplasmic Foci 0.0112 0.0003 0.0074 0.0025 0.0096 0.0027 0.0014 0.0001 0.0003 0.0001 0 0 0.0012 0.0001 0.0003 0.0001 0.0002 0.0001
Eisosomes 0.0001 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0037 0.0003 0.0012 0.0002 0.0021 0.0021 0.0006 0.0008 0.0006 0.0002 0.0001 0.0004 0.0003 0.0004 0.0005 0.0001 0.0001 0.0003
Endosome 0.0459 0.0116 0.0351 0.0167 0.0496 0.0191 0.0269 0.0122 0.0208 0.0163 0.0051 0.0082 0.0193 0.0088 0.0169 0.0123 0.016 0.0104
Golgi 0.0067 0.0003 0.004 0.0047 0.008 0.0032 0.0013 0.0001 0.0008 0.0006 0 0.0001 0.0007 0.0001 0.0005 0.0002 0.0003 0.0001
Lipid Particles 0.0142 0.0014 0.0087 0.002 0.015 0.0044 0.0041 0.0003 0.0045 0.0021 0.0001 0.0009 0.0075 0.0006 0.0036 0.0006 0.0026 0.0008
Mitochondria 0.0154 0.0016 0.0178 0.0013 0.023 0.01 0.0098 0.0005 0.0265 0.0073 0.0001 0.0059 0.0088 0.0007 0.0063 0.0027 0.0006 0.0008
None 0.0027 0 0.0014 0 0.0008 0.0003 0.0006 0 0.0002 0 0 0 0.0002 0.0003 0 0 0 0
Nuclear Periphery 0.0141 0.0163 0.0078 0.0018 0.006 0.0099 0.0071 0.0202 0.0152 0.0011 0.0022 0.0062 0.0053 0.004 0.008 0.0008 0.0013 0.0094
Nucleolus 0.0065 0.0008 0.0102 0.0016 0.0016 0.0018 0.0028 0.0002 0.0043 0.0003 0.0003 0.0004 0.0046 0.0025 0.0054 0.0004 0.0005 0.0023
Nucleus 0.0062 0.002 0.0049 0.001 0.0047 0.006 0.0047 0.0004 0.0031 0.0003 0.0005 0.0007 0.0069 0.0041 0.0045 0.0006 0.0005 0.0016
Peroxisomes 0.0059 0 0.0035 0.006 0.0065 0.0013 0.0006 0 0.0005 0.0001 0 0 0.0023 0 0.0002 0.0001 0.0001 0
Punctate Nuclear 0.0025 0 0.0006 0.0001 0.0026 0.0027 0.0006 0 0 0 0 0 0.0002 0 0 0 0 0
Vacuole 0.3629 0.3761 0.3505 0.4049 0.3545 0.3109 0.338 0.2868 0.3151 0.3082 0.2399 0.2431 0.4083 0.3645 0.4102 0.4896 0.3554 0.2943
Vacuole Periphery 0.4835 0.586 0.5373 0.5393 0.4937 0.6173 0.5938 0.677 0.6057 0.6628 0.7512 0.7329 0.5309 0.6111 0.5398 0.4909 0.6213 0.6788

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.3829 7.1998 16.9182 14.7732 7.8318 16.0992 16.6827 17.6061 13.251 12.5603
Translational Efficiency 0.8261 1.6465 0.7547 0.8971 1.0267 0.3467 0.5801 0.7435 0.8923 0.7287

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
Localization
Cell Percentages vacuolar membrane (67%)
Cell Cycle Regulation No
Subcompartmental Group vac memb-2

YPL162C

YPL162C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypl162c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available