Standard name | |
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Human Ortholog | |
Description | Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 3 | 2 |
Bud | 0 | 0 | – | 0 | 0 | 5 | 0 | – | 3 | 4 | 9 | 6 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 4 | 5 |
Bud Neck | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 3 |
Bud Site | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | – | 0 | 1 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 88 | 81 | – | 43 | 24 | 46 | 84 | – | 78 | 70 | 63 | 79 | 48 | 137 | 157 | 89 | 46 | 52 | 87 | 84 | 20 | 99 | 177 | 111 |
Endoplasmic Reticulum | 0 | 4 | – | 0 | 0 | 0 | 1 | – | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 6 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 1 |
Endosome | 9 | 0 | – | 10 | 6 | 7 | 29 | – | 16 | 16 | 6 | 19 | 4 | 22 | 41 | 4 | 2 | 7 | 13 | 4 | 0 | 3 | 15 | 20 |
Golgi | 1 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 2 |
Mitochondria | 8 | 12 | – | 2 | 0 | 16 | 6 | – | 36 | 53 | 45 | 44 | 4 | 0 | 1 | 0 | 2 | 2 | 4 | 2 | 0 | 2 | 5 | 7 |
Nucleus | 13 | 17 | – | 2 | 2 | 0 | 2 | – | 4 | 7 | 10 | 10 | 9 | 3 | 9 | 20 | 6 | 2 | 32 | 41 | 5 | 23 | 25 | 17 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 1 | 1 | 1 | 2 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 1 | 0 | 1 | 2 | – | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 1 | 1 | – | 2 | 4 | 3 | 11 | – | 6 | 11 | 4 | 1 | 0 | 5 | 7 | 0 | 0 | 0 | 9 | 5 | 0 | 3 | 7 | 8 |
Vac/Vac Membrane | 65 | 49 | – | 17 | 24 | 10 | 34 | – | 20 | 14 | 10 | 9 | 46 | 125 | 111 | 58 | 60 | 58 | 29 | 31 | 3 | 17 | 26 | 17 |
Unique Cell Count | 153 | 141 | 66 | 54 | 75 | 148 | 138 | 146 | 126 | 150 | 99 | 245 | 256 | 141 | 103 | 105 | 189 | 180 | 32 | 162 | 282 | 207 | ||
Labelled Cell Count | 185 | 164 | 77 | 61 | 90 | 170 | 165 | 180 | 151 | 173 | 113 | 297 | 330 | 177 | 118 | 123 | 189 | 180 | 32 | 162 | 282 | 207 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 4.6 | – | 5.6 | 6.6 | 4.8 | 5.9 | – | 4.9 | 5.0 | 5.0 | 5.2 | 5.9 | 6.8 | 7.1 | 8.8 | 9.4 | 9.2 | 5.2 | 5.5 | 6.4 |
Std Deviation (1e-4) | 0.7 | 0.8 | – | 1.2 | 1.1 | 2.2 | 1.5 | – | 1.0 | 1.3 | 1.8 | 1.5 | 1.4 | 1.4 | 1.3 | 1.5 | 1.6 | 1.5 | 1.1 | 1.5 | 2.2 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2911 | 0.1351 | -0.417 | -0.6813 | -0.9217 | -0.2526 | 1.5208 | 1.4555 | 1.1328 | 0.8555 | 0.8862 | 1.209 | 2.7451 | 3.649 | 3.064 | 3.269 | 2.6938 | 3.2206 |
Actin | 0.0257 | 0.0002 | 0.004 | 0.0037 | 0.0057 | 0.0051 | 0.0155 | 0.0006 | 0.016 | 0.0012 | 0.0034 | 0.0149 | 0.0085 | 0.0004 | 0.0087 | 0.0079 | 0.0029 | 0.0058 |
Bud | 0.0024 | 0.0001 | 0.002 | 0.0021 | 0.0003 | 0.0015 | 0.0008 | 0.001 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0002 | 0.0002 |
Bud Neck | 0.0049 | 0.0006 | 0.0013 | 0.0007 | 0.001 | 0.0027 | 0.0031 | 0.0006 | 0.001 | 0.0009 | 0.001 | 0.0051 | 0.0022 | 0.0005 | 0.001 | 0.0011 | 0.002 | 0.0036 |
Bud Periphery | 0.005 | 0 | 0.0007 | 0.0006 | 0.0006 | 0.0008 | 0.0008 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0047 | 0.0013 | 0.0027 | 0.0004 | 0.0007 | 0.0003 | 0.0067 | 0.0099 | 0.0066 | 0.0012 | 0.0007 | 0.0011 | 0.001 | 0.0011 | 0.0017 | 0.0003 | 0.0004 | 0.0002 |
Cell Periphery | 0.0004 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.1729 | 0.3246 | 0.2041 | 0.1965 | 0.2128 | 0.1995 | 0.3055 | 0.442 | 0.3117 | 0.2824 | 0.2305 | 0.2548 | 0.256 | 0.2403 | 0.2244 | 0.1984 | 0.2749 | 0.2204 |
Cytoplasmic Foci | 0.0218 | 0.0175 | 0.0186 | 0.0389 | 0.01 | 0.0201 | 0.0477 | 0.0377 | 0.0141 | 0.03 | 0.0259 | 0.0174 | 0.01 | 0.0112 | 0.0133 | 0.0124 | 0.0197 | 0.0206 |
Eisosomes | 0.0004 | 0 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0101 | 0.0059 | 0.0048 | 0.0035 | 0.0032 | 0.0043 | 0.0103 | 0.0069 | 0.0049 | 0.0034 | 0.0022 | 0.0028 | 0.0056 | 0.0029 | 0.0047 | 0.0031 | 0.0068 | 0.0034 |
Endosome | 0.0362 | 0.0182 | 0.015 | 0.0109 | 0.029 | 0.0144 | 0.0593 | 0.0123 | 0.0116 | 0.0293 | 0.0409 | 0.0101 | 0.0127 | 0.0029 | 0.0121 | 0.0058 | 0.0432 | 0.0128 |
Golgi | 0.0059 | 0.0004 | 0.0022 | 0.0055 | 0.0173 | 0.0031 | 0.0115 | 0.0007 | 0.004 | 0.0064 | 0.0377 | 0.0047 | 0.0016 | 0.0002 | 0.0028 | 0.0012 | 0.001 | 0.0012 |
Lipid Particles | 0.0057 | 0.0007 | 0.0037 | 0.0086 | 0.0354 | 0.0047 | 0.0193 | 0.0004 | 0.0044 | 0.0018 | 0.0292 | 0.0024 | 0.0049 | 0.0001 | 0.0039 | 0.0016 | 0.0027 | 0.001 |
Mitochondria | 0.0037 | 0.0005 | 0.0074 | 0.0035 | 0.021 | 0.0052 | 0.0064 | 0.0006 | 0.0013 | 0.0032 | 0.0774 | 0.0087 | 0.0013 | 0.0006 | 0.0027 | 0.0008 | 0.0013 | 0.0007 |
None | 0.0514 | 0.1452 | 0.1278 | 0.2066 | 0.0629 | 0.093 | 0.0836 | 0.1117 | 0.1382 | 0.0417 | 0.1514 | 0.0552 | 0.1179 | 0.1883 | 0.1682 | 0.1308 | 0.0712 | 0.0875 |
Nuclear Periphery | 0.0483 | 0.0249 | 0.0332 | 0.0182 | 0.0397 | 0.0218 | 0.0475 | 0.0159 | 0.0162 | 0.0154 | 0.0118 | 0.0134 | 0.0241 | 0.0155 | 0.0143 | 0.0139 | 0.0198 | 0.0096 |
Nucleolus | 0.005 | 0.0027 | 0.0032 | 0.0023 | 0.0043 | 0.0034 | 0.0025 | 0.0011 | 0.001 | 0.0011 | 0.0043 | 0.0028 | 0.0019 | 0.0018 | 0.0026 | 0.0015 | 0.0021 | 0.0029 |
Nucleus | 0.5339 | 0.4355 | 0.532 | 0.4395 | 0.4798 | 0.5643 | 0.3186 | 0.342 | 0.4324 | 0.5479 | 0.3279 | 0.555 | 0.5237 | 0.512 | 0.515 | 0.5938 | 0.5054 | 0.5853 |
Peroxisomes | 0.0087 | 0.0004 | 0.0051 | 0.0204 | 0.0082 | 0.0017 | 0.0245 | 0.0011 | 0.0134 | 0.0056 | 0.0192 | 0.0169 | 0.002 | 0.001 | 0.0029 | 0.0037 | 0.0022 | 0.0056 |
Punctate Nuclear | 0.0424 | 0.0172 | 0.0284 | 0.036 | 0.0619 | 0.0507 | 0.0257 | 0.0115 | 0.019 | 0.0254 | 0.0332 | 0.0302 | 0.0199 | 0.0189 | 0.017 | 0.0216 | 0.0398 | 0.0369 |
Vacuole | 0.0078 | 0.0035 | 0.0027 | 0.0012 | 0.0027 | 0.0023 | 0.0075 | 0.003 | 0.0027 | 0.0019 | 0.0019 | 0.0031 | 0.0045 | 0.0017 | 0.0026 | 0.0016 | 0.0034 | 0.0017 |
Vacuole Periphery | 0.0028 | 0.0006 | 0.0009 | 0.0005 | 0.0032 | 0.001 | 0.0026 | 0.0004 | 0.0005 | 0.0007 | 0.0008 | 0.0005 | 0.001 | 0.0003 | 0.0007 | 0.0003 | 0.0007 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | – | – | – | – | – | – | – | – | – | – |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | – |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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