Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.09 0 0 0 0.05 0 0 0 0 0.11 0.14 0.17 0.09 0.09 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0.06 0.06 0 0 0.08 0.09 0.09 0.16 0.18 0.14 0.14 0 0 0 0.11 0 0.07 0 0 0 0 0 0
Nucleus 0.86 0.9 0.89 0.84 0.87 0.79 0.74 0.69 0.65 0.62 0.63 0.61 0.83 0.8 0.76 0.8 0.82 0.89 0.77 0.81 0.72 0.62 0.66 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.44 0.29 0.31 0.21 0.2 0.35 0.38 0.44 0.53 0.52 0.51 0.6 0.19 0.17 0.19 0 0 0 0.16 0.12 0.19 0.26 0.22 0.19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3 3 4 2 2 0 0 0 1 0 0 0 4 0 0 4 5
Bud Neck 0 0 0 0 0 2 1 4 1 0 1 1 0 0 1 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 5 5 3 15 22 17 25 1 2 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 6 10 13 7 7 9 20 8 6 9 10 30 68 83 23 11 7 0 0 1 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 2 1 0 0 1 0 0 0 1 0 4 5 1 0 0 1 0 0 1
Endosome 0 0 0 0 1 0 0 0 0 1 0 0 0 2 4 9 2 1 2 3 1 1 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 2
Mitochondria 27 16 13 2 9 30 41 38 59 92 49 60 3 5 1 27 4 7 0 1 2 0 1 5
Nucleus 147 249 206 118 215 285 339 306 233 320 217 263 218 377 378 201 97 87 136 240 176 87 180 189
Nuclear Periphery 0 1 1 2 0 2 5 3 4 2 9 2 2 2 0 2 1 0 0 0 0 1 0 1
Nucleolus 75 80 71 29 50 125 175 194 191 268 176 259 51 79 92 3 0 0 27 36 45 36 59 57
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 1 3 5 0 1 0 1 0 0 0 1 0
SpindlePole 1 0 0 0 0 0 0 2 1 1 1 1 3 5 5 1 1 1 1 1 1 1 3 4
Vac/Vac Membrane 1 4 2 0 2 1 4 0 1 3 0 0 1 1 3 12 4 3 3 3 8 4 13 27
Unique Cell Count 170 277 232 140 247 361 459 442 360 514 346 430 263 470 496 250 119 98 178 296 245 140 273 306
Labelled Cell Count 253 356 303 164 284 459 580 573 516 720 482 623 310 545 574 283 126 107 178 296 245 140 273 306


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 7.3 7.5 7.3 7.4 6.5 6.3 6.6 6.1 6.0 6.4 6.2 8.0 7.4 7.5 5.7 6.8 7.2 7.3 7.3 8.0
Std Deviation (1e-4) 1.4 1.6 2.2 1.8 2.0 2.0 2.0 1.9 1.6 1.8 2.2 1.9 2.3 2.4 2.5 1.3 1.6 1.3 2.2 2.0 1.8
Intensity Change (Log2) – – – -0.03 -0.02 -0.2 -0.25 -0.19 -0.28 -0.31 -0.22 -0.27 0.09 -0.01 0.01 -0.39 -0.13 -0.05 -0.03 -0.04 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 3.1 3.2 3.4 3.7 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 -0.9 -1.7 -1.8 0.1 -1.4 0 -1.1 -1.4 2.9 4.0 4.7 2.1 1.8 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -2.0 -1.0 1.2 1.5 1.4 3.9 4.5 3.3 3.3 -2.8 -3.6 0 2.1 -0.9 0.5
Nucleus -1.3 -0.6 -3.1 -4.5 -5.6 -6.5 -7.4 -6.9 -7.5 -1.9 -2.9 -4.0 -2.5 -1.9 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.1 -2.6 1.0 2.0 3.4 5.4 5.5 4.8 7.3 -2.9 -4.2 -3.6 -8.9 -6.8 -6.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.6 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2695 2.2973 2.4818 1.7437 1.6932 2.2245 4.5264 3.8159 3.6983 3.285 3.2212 3.7297 5.5534 5.2963 4.6846 3.9617 5.5709 4.1802
Actin 0.0107 0 0.0167 0.0007 0.015 0.0003 0.0174 0 0.017 0 0.0032 0.0041 0.0105 0.004 0.0258 0 0.0022 0.0139
Bud 0.0002 0.0001 0.0006 0.0001 0.0014 0.0001 0.0007 0 0.0004 0 0.0001 0 0.0004 0.0002 0.0002 0 0.0002 0.0002
Bud Neck 0.0008 0.0002 0.0006 0.0001 0.0049 0.0009 0.0028 0.0001 0.0006 0.0001 0.0023 0.0006 0.0005 0.0002 0.0001 0.0002 0.0005 0.0007
Bud Periphery 0.0005 0.0002 0.001 0.0001 0.0045 0.0001 0.0018 0 0.0011 0 0.0001 0.0001 0.0007 0.0001 0.0002 0 0.0003 0.0002
Bud Site 0.0022 0.0002 0.0076 0.0005 0.0033 0.0001 0.0031 0.0001 0.0016 0 0.0002 0.0001 0.0008 0.0048 0.0003 0.0002 0.0013 0.0003
Cell Periphery 0.0001 0.0002 0.0005 0 0.0005 0.0001 0.0004 0 0.0003 0 0.0003 0 0.0005 0.0001 0 0 0.0002 0
Cytoplasm 0.0011 0.0001 0.0025 0.0006 0.0007 0 0.0024 0 0.0027 0 0.0013 0.0001 0.0193 0.0025 0.0003 0.0001 0.0197 0.0013
Cytoplasmic Foci 0.0078 0 0.0025 0.033 0.0024 0.0001 0.0049 0 0.005 0 0.0004 0.0002 0.0164 0.0114 0.008 0 0.0428 0.0031
Eisosomes 0.0001 0 0.0002 0 0.0004 0 0.0002 0 0.0002 0 0.0055 0.0001 0.0001 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0004 0 0.001 0.0004 0.0033 0 0.0033 0 0.001 0 0.0008 0.0012 0.0038 0.0013 0.0007 0 0.0065 0.0003
Endosome 0.0134 0.0001 0.0063 0.0217 0.0213 0.0002 0.0113 0 0.0033 0 0.0006 0.0009 0.042 0.0011 0.0089 0.0001 0.0624 0.0083
Golgi 0.0023 0 0.0021 0.007 0.0063 0.0003 0.0034 0 0.0021 0 0.0006 0.0009 0.0174 0.0013 0.014 0 0.0179 0.0074
Lipid Particles 0.0052 0.0001 0.0033 0.0319 0.0076 0.0006 0.0079 0 0.0093 0 0.0012 0.0015 0.0139 0.0026 0.0074 0.0001 0.0079 0.0114
Mitochondria 0.0028 0.0038 0.0141 0.0007 0.0298 0.0098 0.0065 0 0.0082 0.0002 0.0007 0.0004 0.0304 0.0007 0.0027 0.0002 0.0222 0.0044
None 0.001 0.0001 0.0019 0.0003 0.0017 0 0.0035 0 0.0021 0 0.034 0.0002 0.0175 0.0015 0.0002 0 0.0069 0.0002
Nuclear Periphery 0.0035 0.0006 0.0052 0.0017 0.0321 0.0001 0.0133 0.0002 0.0043 0.0004 0.0067 0.0014 0.0234 0.0018 0.0005 0.0015 0.0015 0.0015
Nucleolus 0.0676 0.0741 0.0356 0.0456 0.2903 0.1533 0.0572 0.0366 0.034 0.0392 0.1505 0.0962 0.0319 0.0338 0.0161 0.0675 0.181 0.0863
Nucleus 0.865 0.9181 0.8875 0.8299 0.5559 0.8317 0.8386 0.961 0.8915 0.9587 0.7642 0.8888 0.7286 0.9146 0.8979 0.9256 0.6154 0.8477
Peroxisomes 0.0085 0 0.0009 0.0064 0.0022 0.0015 0.003 0 0.0077 0 0.0003 0.0011 0.0091 0.0129 0.0152 0 0.0068 0.001
Punctate Nuclear 0.0024 0.0005 0.0041 0.0189 0.0097 0.0005 0.0123 0.002 0.0046 0.0011 0.0266 0.0019 0.0122 0.0048 0.001 0.0043 0.002 0.0109
Vacuole 0.0033 0.0007 0.0044 0.0003 0.0032 0.0002 0.0037 0 0.0019 0 0.0002 0.0001 0.0143 0.0003 0.0001 0.0001 0.0014 0.0003
Vacuole Periphery 0.0011 0.001 0.0014 0.0002 0.0034 0 0.0022 0 0.0012 0 0.0002 0.0001 0.0061 0.0001 0.0001 0.0001 0.0009 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.6071 15.5641 17.8051 19.6177 20.6926 18.1261 19.0154 19.6937 22.87 17.7414
Translational Efficiency 2.1551 2.3756 1.9019 2.2418 1.6056 1.4651 1.6273 2.0007 1.6585 1.5658

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Component of the Rpd3L histone deacetylase complex; relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Cti6

Cti6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cti6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available