| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 3 | 2 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | – | 0 | 4 | 5 | 1 | 2 | 3 | 13 |
| Bud | 10 | 1 | 7 | 9 | 0 | 5 | – | 6 | 4 | 5 | 8 | 10 | 1 | 0 | 0 | 2 | – | 2 | 2 | 1 | 0 | 1 | 0 | 3 |
| Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 2 |
| Bud Site | 1 | 0 | 3 | 1 | 0 | 2 | – | 1 | 4 | 4 | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
| Cell Periphery | 3 | 6 | 3 | 4 | 2 | 3 | – | 1 | 0 | 4 | 1 | 1 | 3 | 8 | 8 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 16 | 1 | 4 | 6 | 3 | 6 | – | 6 | 3 | 2 | 3 | 2 | 0 | 4 | 8 | 4 | – | 2 | 11 | 0 | 0 | 0 | 0 | 0 |
| Endoplasmic Reticulum | 3 | 2 | 2 | 3 | 3 | 2 | – | 2 | 3 | 3 | 3 | 2 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Endosome | 7 | 1 | 1 | 0 | 13 | 5 | – | 5 | 1 | 0 | 0 | 1 | 8 | 14 | 13 | 0 | – | 0 | 27 | 2 | 10 | 3 | 3 | 9 |
| Golgi | 2 | 19 | 0 | 2 | 1 | 1 | – | 0 | 1 | 0 | 0 | 0 | 15 | 27 | 31 | 6 | – | 7 | 18 | 18 | 6 | 7 | 10 | 17 |
| Mitochondria | 318 | 193 | 202 | 244 | 206 | 274 | – | 323 | 327 | 258 | 300 | 298 | 186 | 256 | 216 | 3 | – | 1 | 268 | 205 | 159 | 111 | 127 | 153 |
| Nucleus | 4 | 0 | 0 | 1 | 0 | 1 | – | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 1 | 0 | 0 |
| Nuclear Periphery | 17 | 11 | 10 | 15 | 23 | 43 | – | 36 | 49 | 38 | 32 | 43 | 22 | 17 | 16 | 0 | – | 2 | 2 | 1 | 1 | 2 | 2 | 2 |
| Nucleolus | 21 | 6 | 23 | 19 | 29 | 52 | – | 53 | 49 | 39 | 36 | 38 | 13 | 7 | 15 | 0 | – | 0 | 2 | 2 | 2 | 3 | 3 | 4 |
| Peroxisomes | 8 | 8 | 5 | 9 | 13 | 4 | – | 5 | 3 | 3 | 3 | 4 | 14 | 14 | 9 | 0 | – | 1 | 13 | 4 | 22 | 7 | 10 | 16 |
| SpindlePole | 2 | 1 | 1 | 1 | 2 | 0 | – | 4 | 4 | 4 | 1 | 0 | 2 | 4 | 2 | 0 | – | 0 | 3 | 0 | 1 | 0 | 1 | 1 |
| Vac/Vac Membrane | 59 | 37 | 8 | 5 | 15 | 10 | – | 15 | 7 | 5 | 7 | 8 | 24 | 24 | 31 | 2 | – | 1 | 19 | 8 | 1 | 4 | 7 | 8 |
| Unique Cell Count | 372 | 220 | 210 | 258 | 244 | 317 | 377 | 344 | 284 | 322 | 317 | 215 | 297 | 266 | 16 | 17 | 380 | 254 | 214 | 149 | 175 | 236 | ||
| Labelled Cell Count | 474 | 288 | 269 | 319 | 311 | 408 | 461 | 455 | 366 | 396 | 409 | 290 | 379 | 351 | 19 | 18 | 380 | 254 | 214 | 149 | 175 | 236 | ||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 7.9 | 13.0 | 7.2 | 8.7 | 8.7 | 8.0 | – | 8.5 | 7.8 | 7.9 | 8.0 | 7.8 | 10.3 | 10.8 | 10.1 | 9.6 | – | 10.5 | 11.6 | 12.8 | 12.6 |
| Std Deviation (1e-4) | 2.0 | 2.6 | 1.9 | 2.9 | 2.2 | 2.2 | – | 2.8 | 2.7 | 2.7 | 2.5 | 2.6 | 2.1 | 2.3 | 2.3 | 2.3 | – | 2.8 | 2.9 | 3.2 | 3.1 |
| Intensity Change (Log2) | – | – | – | 0.27 | 0.28 | 0.15 | – | 0.24 | 0.13 | 0.14 | 0.16 | 0.13 | 0.52 | 0.59 | 0.49 | 0.43 | – | 0.55 | 0.69 | 0.83 | 0.81 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
| Bud | 0.1 | 0 | 0 | – | 0 | 0 | -1.1 | -0.6 | -0.1 | 0 | 0 | 0 | 0 | – | 0 |
| Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
| Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
| Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
| Cytoplasm | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0 | – | 0 |
| Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
| Endosome | 0 | 3.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.8 | 0 | – | 0 |
| Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.9 | 4.5 | 5.1 | 0 | – | 0 |
| Mitochondria | -0.8 | -4.1 | -3.7 | – | -4.0 | -0.6 | -2.3 | -1.5 | -1.1 | -3.5 | -3.8 | -5.0 | -10.3 | – | -11.6 |
| Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
| Nuclear Periphery | 0.5 | 1.9 | 3.3 | – | 2.1 | 3.5 | 3.2 | 2.2 | 3.3 | 2.1 | 0.5 | 0.6 | 0 | – | 0 |
| Nucleolus | -1.4 | 0.3 | 1.8 | – | 1.1 | 1.1 | 0.9 | 0.1 | 0.4 | -1.8 | -4.0 | -2.1 | 0 | – | 0 |
| Peroxisomes | 0.7 | 1.6 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.1 | 1.4 | 0.6 | 0 | – | 0 |
| SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
| Vacuole | -1.2 | 1.1 | -0.4 | – | 0.1 | -1.2 | -1.4 | -1.1 | -0.8 | 2.9 | 1.9 | 3.1 | 0 | – | 0 |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 33.2586 | 31.4801 | 20.7641 | 41.3046 | 35.5116 | 43.0168 | 49.5125 | 38.1916 | 41.7403 | 44.3089 |
| Translational Efficiency | 2.0642 | 1.8567 | 2.9266 | 1.0534 | 1.8543 | 2.4728 | 1.6742 | 2.5411 | 2.3624 | 2.2463 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | – |
| Protein Localization | – |
| Gene Expression | ✘ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress |
| Localization | |
| Cell Percentages | mitochondrion (98%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | mito-2 |
Rtc6
Rtc6
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Rtc6-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
