Standard name | |
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Human Ortholog | |
Description | RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing; Nrd1-Nab3 pathway appears to have a role in rapid suppression of some genes when cells are shifted to poor growth conditions, indicating role for Nrd1-Nab3 in regulating cellular response to nutrient availability |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 1 | 4 | 6 |
Bud | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 9 | – | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 4 |
Bud Neck | 2 | 1 | 6 | 2 | 15 | 14 | 13 | 19 | 9 | – | 6 | 15 | 0 | 0 | 0 | 3 | 3 | 3 | 0 | 2 | 1 | 2 | 3 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 0 | 0 | 3 | 3 | 2 | 0 | – | 0 | 2 | 0 | 0 | 0 | 8 | 3 | 1 | 0 | 3 | 4 | 1 | 1 | 2 |
Cytoplasm | 77 | 301 | 322 | 167 | 294 | 344 | 387 | 519 | 320 | – | 211 | 283 | 66 | 241 | 250 | 772 | 534 | 506 | 64 | 253 | 188 | 58 | 149 | 136 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 59 | 21 | 40 | 1 | 0 | 0 | 1 | 1 | 3 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 4 | 5 | 1 | 1 | 2 | 2 | 1 | 3 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 13 | 6 | 0 | 5 | 1 | 20 | 28 | 11 | 70 | – | 83 | 55 | 7 | 1 | 0 | 18 | 15 | 6 | 9 | 25 | 37 | 19 | 34 | 19 |
Nucleus | 0 | 1 | 1 | 1 | 2 | 1 | 1 | 6 | 6 | – | 5 | 1 | 0 | 0 | 0 | 18 | 8 | 3 | 0 | 0 | 3 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 1 | 6 | 8 | 16 | 4 | – | 6 | 10 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 2 | 1 | 0 | 4 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 3 | – | 0 | 3 | 0 | 0 | 0 | 62 | 44 | 34 | 2 | 10 | 8 | 6 | 14 | 9 |
Unique Cell Count | 86 | 308 | 325 | 168 | 297 | 358 | 403 | 540 | 374 | 258 | 325 | 66 | 243 | 251 | 831 | 579 | 534 | 84 | 312 | 271 | 97 | 222 | 202 | |
Labelled Cell Count | 93 | 318 | 332 | 176 | 313 | 390 | 442 | 574 | 426 | 315 | 372 | 73 | 245 | 252 | 947 | 635 | 594 | 84 | 312 | 271 | 97 | 222 | 202 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 3.6 | 4.3 | 4.1 | 4.8 | 3.6 | 3.8 | 4.2 | 3.3 | – | 3.1 | 3.7 | 3.7 | 3.9 | 3.9 | 5.5 | 5.4 | 5.3 | 3.4 | 3.6 | 4.2 |
Std Deviation (1e-4) | 0.4 | 0.7 | 1.1 | 1.4 | 1.8 | 1.7 | 1.6 | 1.7 | 1.4 | – | 1.5 | 1.6 | 0.7 | 1.0 | 1.5 | 0.9 | 0.9 | 0.9 | 1.1 | 1.1 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.16 | -0.24 | -0.17 | -0.02 | -0.37 | – | -0.45 | -0.21 | -0.22 | -0.13 | -0.13 | 0.36 | 0.34 | 0.31 | -0.34 | -0.25 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.1 | 5.1 | 4.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8352 | 1.3591 | 1.4362 | 1.0929 | 0.7913 | 1.2753 | -0.1106 | 0.3025 | -0.2267 | 0.3591 | 0.0168 | -0.1377 | 0.2418 | -0.0784 | 0.039 | 0.2791 | -0.197 | -0.1083 |
Actin | 0.0199 | 0.0001 | 0.0058 | 0.0015 | 0.0025 | 0.0119 | 0.0277 | 0.0005 | 0.0062 | 0.0081 | 0.0395 | 0.0038 | 0.0337 | 0.0004 | 0.0166 | 0.0606 | 0.0291 | 0.002 |
Bud | 0.0003 | 0.0012 | 0.0003 | 0.0006 | 0.001 | 0.0104 | 0.0023 | 0.002 | 0.003 | 0.0018 | 0.007 | 0.0008 | 0.0012 | 0.0025 | 0.0004 | 0.0035 | 0.0032 | 0.0001 |
Bud Neck | 0.0027 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0008 | 0.0014 | 0.0002 | 0.001 | 0.0004 | 0.0025 | 0.0009 | 0.0034 | 0.0001 | 0.0002 | 0.0014 | 0.0002 | 0.0004 |
Bud Periphery | 0.0008 | 0.0008 | 0.0005 | 0.0005 | 0.0034 | 0.0047 | 0.0049 | 0.0033 | 0.0031 | 0.003 | 0.0075 | 0.0017 | 0.0047 | 0.0043 | 0.0006 | 0.005 | 0.0043 | 0.0002 |
Bud Site | 0.0015 | 0.0041 | 0.0015 | 0.0002 | 0.0041 | 0.0024 | 0.0149 | 0.0038 | 0.0141 | 0.0009 | 0.0012 | 0.0004 | 0.0097 | 0.0036 | 0.0012 | 0.0064 | 0.0047 | 0.0004 |
Cell Periphery | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0009 | 0.0002 | 0.0007 | 0.0005 | 0.0008 | 0.0001 | 0.0005 | 0.0001 | 0.0006 | 0.0003 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Cytoplasm | 0.1224 | 0.1469 | 0.1669 | 0.1325 | 0.0745 | 0.1332 | 0.2168 | 0.2496 | 0.191 | 0.3628 | 0.2098 | 0.2186 | 0.3455 | 0.3313 | 0.3498 | 0.1355 | 0.4036 | 0.3834 |
Cytoplasmic Foci | 0.0165 | 0.0019 | 0.0203 | 0.0109 | 0.0116 | 0.0249 | 0.0318 | 0.0075 | 0.0228 | 0.0186 | 0.0341 | 0.0222 | 0.0249 | 0.0099 | 0.01 | 0.0159 | 0.0152 | 0.0132 |
Eisosomes | 0.0017 | 0.0002 | 0.0012 | 0.0004 | 0.0004 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0007 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0022 | 0.0009 | 0.0019 | 0.0051 | 0.0034 | 0.0019 | 0.0056 | 0.002 | 0.0018 | 0.0021 | 0.002 | 0.0024 | 0.0131 | 0.0007 | 0.0088 | 0.0023 | 0.0018 | 0.0023 |
Endosome | 0.009 | 0.0009 | 0.0103 | 0.0124 | 0.0387 | 0.0118 | 0.0283 | 0.006 | 0.0219 | 0.0221 | 0.026 | 0.0195 | 0.0336 | 0.0033 | 0.0226 | 0.0427 | 0.0308 | 0.0172 |
Golgi | 0.0058 | 0.0001 | 0.0048 | 0.0021 | 0.0138 | 0.0062 | 0.0061 | 0.0009 | 0.0064 | 0.007 | 0.0084 | 0.0017 | 0.0085 | 0.0004 | 0.0032 | 0.0218 | 0.002 | 0.0011 |
Lipid Particles | 0.0056 | 0.0002 | 0.0064 | 0.0271 | 0.022 | 0.0067 | 0.0167 | 0.0002 | 0.004 | 0.0029 | 0.0067 | 0.0011 | 0.0145 | 0.0005 | 0.0011 | 0.02 | 0.0004 | 0.001 |
Mitochondria | 0.0028 | 0.0004 | 0.0134 | 0.0016 | 0.0202 | 0.0147 | 0.0104 | 0.0009 | 0.0081 | 0.0028 | 0.0052 | 0.0024 | 0.0129 | 0.0004 | 0.0015 | 0.0815 | 0.0015 | 0.0007 |
None | 0.7827 | 0.8332 | 0.7486 | 0.7868 | 0.7673 | 0.7494 | 0.6023 | 0.7051 | 0.6968 | 0.5444 | 0.6135 | 0.7087 | 0.4666 | 0.6371 | 0.5697 | 0.5842 | 0.4912 | 0.5696 |
Nuclear Periphery | 0.0011 | 0.0002 | 0.0004 | 0.0011 | 0.0037 | 0.0012 | 0.0038 | 0.0016 | 0.0012 | 0.0007 | 0.0012 | 0.0065 | 0.0071 | 0.0003 | 0.0034 | 0.0018 | 0.0006 | 0.0029 |
Nucleolus | 0.0006 | 0.0005 | 0.0004 | 0.0008 | 0.0019 | 0.0006 | 0.0007 | 0.0002 | 0.0011 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0003 |
Nucleus | 0.0007 | 0.0046 | 0.0007 | 0.0033 | 0.0061 | 0.0024 | 0.0023 | 0.0122 | 0.0023 | 0.0011 | 0.0025 | 0.0012 | 0.0022 | 0.0008 | 0.001 | 0.0013 | 0.0005 | 0.0013 |
Peroxisomes | 0.0194 | 0.0001 | 0.0099 | 0.0015 | 0.0075 | 0.0073 | 0.0134 | 0.0001 | 0.0071 | 0.0155 | 0.0234 | 0.0014 | 0.0069 | 0.0005 | 0.0005 | 0.0072 | 0.0049 | 0.0004 |
Punctate Nuclear | 0.0015 | 0.0013 | 0.0028 | 0.0086 | 0.0008 | 0.0075 | 0.0033 | 0.0004 | 0.0035 | 0.001 | 0.0039 | 0.0039 | 0.0036 | 0.0007 | 0.0004 | 0.0007 | 0.0007 | 0.0014 |
Vacuole | 0.0015 | 0.0018 | 0.0029 | 0.0017 | 0.0117 | 0.0011 | 0.0047 | 0.0025 | 0.0028 | 0.0036 | 0.003 | 0.0014 | 0.0055 | 0.0019 | 0.0068 | 0.0042 | 0.0038 | 0.0015 |
Vacuole Periphery | 0.0004 | 0.0002 | 0.0005 | 0.0006 | 0.0042 | 0.0005 | 0.0014 | 0.0004 | 0.0009 | 0.0009 | 0.001 | 0.0009 | 0.0012 | 0.0003 | 0.0016 | 0.0025 | 0.0013 | 0.0005 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 51.2998 | 61.4182 | 54.3861 | 50.1828 | 43.9457 | 51.2714 | 58.5809 | 62.311 | 53.3832 | 48.9219 |
Translational Efficiency | 2.5922 | 2.489 | 2.1455 | 2.3202 | 2.2731 | 2.3071 | 1.9194 | 1.7493 | 1.8781 | 1.9436 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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