Standard name
Human Ortholog
Description DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.12 0.13 0.12 0.15 0.13 0.18 0.11 0.12 0.1 0.13 0.18 0.19 0.27 0.1 0.12 0.08 0 0 0 0 0.05 0.06
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0 0 0.06 0 0.23 0.25 0.25 0.41 0.32 0.65 0.42 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.77 0.85 0.84 0.84 0.78 0.68 0.71 0.64 0.67 0.66 0.55 0.63 0.79 0.66 0.65 0.81 0.81 0.82 0.75 0.79 0.64 0.6 0.62 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.18 0.05 0 0.09 0.07 0.07 0 0.05 0.08 0.05 0.08 0.06 0.09 0.06 0.08 0.08 0.11 0.15 0.11 0.22 0.19 0.18 0.16
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 0 0 0 0 1 5 1 0 7 5 5 0 0 0 2 0 0 3 4 3 1 2 1
Bud Neck 2 0 1 0 1 1 1 5 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2 0 0 0 0 2 5 3 8 2 10 13 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 15 7 17 25 35 45 49 64 36 47 42 49 14 17 27 38 42 28 7 4 5 1 4 8
Endoplasmic Reticulum 2 1 0 0 2 0 0 1 0 0 1 0 0 1 1 17 12 8 0 0 1 1 0 1
Endosome 1 0 0 1 4 2 3 3 2 4 0 2 0 2 1 11 2 3 4 4 2 1 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1 1 0 0 0 0
Mitochondria 75 10 0 11 8 70 90 88 131 122 261 164 1 2 1 27 9 7 4 3 3 0 1 3
Nucleus 231 210 121 167 233 206 257 223 212 252 219 243 61 59 65 321 291 276 266 218 121 32 60 78
Nuclear Periphery 5 0 0 1 0 1 2 0 0 0 1 1 0 0 0 1 0 0 1 2 1 0 0 0
Nucleolus 4 0 0 0 0 0 0 0 0 0 1 1 1 1 1 0 0 0 1 0 1 0 1 2
Peroxisomes 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 4 11 5 8 7 4 12 3 8 2 3 2 3 3 2 5 3 4 0 2 2
Vac/Vac Membrane 17 45 7 7 28 22 24 14 17 29 20 32 5 8 6 33 29 37 54 29 41 10 17 20
Unique Cell Count 299 246 144 199 297 301 364 349 318 382 401 387 77 89 100 395 359 337 357 277 191 54 97 131
Labelled Cell Count 356 273 148 216 322 355 445 409 412 477 563 518 85 93 104 455 389 362 357 277 191 54 97 131


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 5.2 5.7 5.1 5.8 4.7 4.9 4.9 4.2 4.5 3.8 4.6 5.8 6.1 6.3 6.8 6.9 6.5 – 5.6 6.3
Std Deviation (1e-4) 0.7 0.9 1.1 1.1 1.4 1.4 1.3 1.4 0.9 1.0 1.2 1.8 1.5 1.6 2.0 1.3 1.5 1.2 – 1.7 1.8
Intensity Change (Log2) – – – -0.15 0.03 -0.26 -0.21 -0.19 -0.44 -0.32 -0.56 -0.3 0.04 0.1 0.16 0.27 0.28 0.21 – -0.0 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 0 0.9 0.5 1.8 -0.2 0.2 -0.4 0.3 1.3 1.5 3.0 -0.7 0 -1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 6.6 0 0 0 0 0 0 0 3.2 0 0
Nucleus 0 -1.4 -3.5 -3.1 -4.4 -3.9 -4.1 -6.3 -4.7 -0.9 -3.1 -3.4 -0.7 -0.8 -0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.6 1.7 1.0 0.7 -0.4 0.2 1.1 0.1 1.3 0 1.2 0.4 1.4 1.3 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5338 2.8838 2.8144 2.5344 2.2312 2.8685 3.1562 2.4074 1.9007 1.3695 1.285 1.5798 3.1689 2.7843 2.7556 3.2456 2.5601 2.7061
Actin 0.011 0 0.0008 0.0004 0.0002 0.0005 0.0373 0.0016 0.0198 0.001 0.0003 0.0002 0.0218 0.0002 0.0205 0.0036 0.0001 0.0002
Bud 0.0008 0 0.0001 0.0002 0.0001 0.0001 0.0019 0.0001 0.0008 0.0001 0.0002 0.0001 0.0019 0.0001 0.0003 0.0013 0 0.0031
Bud Neck 0.0007 0.0002 0.0002 0.0008 0.0014 0.0024 0.0119 0.0003 0.0036 0.0009 0.0007 0.0015 0.0033 0.0004 0.0025 0.0005 0.0014 0.0051
Bud Periphery 0.0027 0 0.0003 0.0002 0.0001 0.0003 0.0052 0.0001 0.0006 0.0001 0.0002 0.0001 0.003 0 0.0007 0.0015 0 0.0008
Bud Site 0.0006 0.0004 0.0002 0.0001 0.0002 0.0002 0.0049 0.0005 0.008 0.0003 0.0003 0.0002 0.003 0.0007 0.0033 0.0021 0.0005 0.0004
Cell Periphery 0.0002 0 0 0 0 0 0.0008 0.0001 0.0002 0 0 0 0.0002 0.0001 0.0005 0.0001 0.0001 0
Cytoplasm 0.0152 0.0088 0.0235 0.0214 0.0434 0.0149 0.017 0.051 0.0764 0.052 0.1107 0.0224 0.0497 0.0802 0.0288 0.0857 0.03 0.019
Cytoplasmic Foci 0.0072 0.0001 0.0005 0.0027 0.0005 0.0006 0.0238 0.0065 0.0167 0.0011 0.0012 0.0074 0.0127 0.0036 0.0038 0.0096 0.001 0.0021
Eisosomes 0.0004 0 0 0 0 0.0001 0.0023 0.0001 0.0001 0 0 0 0.0001 0.0001 0.0002 0.0001 0 0
Endoplasmic Reticulum 0.0044 0.0005 0.0037 0.0045 0.004 0.0007 0.0088 0.0019 0.0018 0.0017 0.0013 0.0005 0.0042 0.0016 0.004 0.0021 0.0016 0.0008
Endosome 0.0232 0.0003 0.0015 0.0165 0.0034 0.0011 0.0377 0.0109 0.0218 0.0032 0.0009 0.0051 0.0196 0.0028 0.0062 0.0115 0.0009 0.0052
Golgi 0.0032 0 0.0002 0.0005 0 0.0001 0.027 0.0226 0.0134 0.0001 0 0.0013 0.0059 0.0002 0.0052 0.0083 0 0.0005
Lipid Particles 0.0048 0 0.0001 0.0079 0.0001 0.0003 0.0141 0.0038 0.0013 0.0002 0 0.0069 0.0053 0.0005 0.0092 0.0027 0.0003 0.0002
Mitochondria 0.0116 0.0002 0.0019 0.0011 0.0006 0.0014 0.0226 0.0021 0.027 0.0011 0.0011 0.0011 0.0054 0.0004 0.0102 0.0058 0.0005 0.0003
None 0.1821 0.0113 0.0866 0.0567 0.0093 0.0667 0.1567 0.1595 0.0391 0.0317 0.2469 0.0723 0.0666 0.0965 0.0617 0.1753 0.0761 0.0231
Nuclear Periphery 0.0497 0.0087 0.0232 0.0353 0.0365 0.0145 0.123 0.0407 0.0173 0.0803 0.0046 0.0102 0.0325 0.0133 0.0146 0.018 0.0037 0.0052
Nucleolus 0.0071 0.006 0.0069 0.0059 0.0105 0.0127 0.0103 0.0052 0.0057 0.0095 0.0055 0.0131 0.0087 0.0065 0.0041 0.0046 0.0098 0.0083
Nucleus 0.612 0.9556 0.8275 0.791 0.8587 0.8604 0.3771 0.6695 0.6974 0.7166 0.6173 0.8322 0.6858 0.7688 0.801 0.6499 0.8559 0.9117
Peroxisomes 0.0019 0 0 0.0004 0 0.0001 0.0149 0.0012 0.032 0.0001 0.0001 0.0032 0.0059 0.0001 0.003 0.0028 0 0.0002
Punctate Nuclear 0.0446 0.0073 0.02 0.0476 0.0263 0.0216 0.0887 0.0184 0.0109 0.0975 0.0074 0.021 0.0585 0.0219 0.0179 0.0116 0.017 0.013
Vacuole 0.0081 0.0005 0.001 0.0045 0.0038 0.0006 0.006 0.0018 0.005 0.0016 0.0009 0.001 0.0044 0.0019 0.0015 0.0021 0.0008 0.0007
Vacuole Periphery 0.0084 0.0001 0.0018 0.0022 0.0008 0.0008 0.0081 0.0021 0.0007 0.0009 0.0003 0.0003 0.0013 0.0003 0.0006 0.0009 0.0001 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 7.5 1.0727 3.9801 7.7893 8.5572 2.9984 5.4232 8.7847 6.7828 9.0275
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress
Localization
Cell Percentages nucleus (63%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ddc1

Ddc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ddc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available