Standard name
Human Ortholog
Description Delta adaptin-like subunit of the clathrin associated protein complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; suppressor of loss of casein kinase 1 function; the clathrin associated protein complex is also known as AP-3

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.12 0.2 0 0 0 0 0 0.05 0.05 0 0 0 0.19 0.21 0.16 0.11 0.08 0.12 0.05 0.05 0.09 0.05 0.05 0.06
Bud 0 0 0 0 0 0 0.06 0.06 0.1 0.05 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0.05 0.14 0.11 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.1 0 0 0 0 0 0 0 0 0 0 0.16 0.13 0.12 0.05 0 0 0 0 0 0 0 0
Endosome 0.18 0 0.18 0.15 0.16 0.11 0.16 0.18 0.08 0.05 0 0.11 0.14 0.14 0.13 0.27 0.32 0.25 0.17 0.19 0.13 0.14 0.12 0.1
Golgi 0.62 0.27 0.49 0.31 0.33 0.08 0.08 0.06 0.06 0 0 0 0.54 0.57 0.64 0.35 0.38 0.54 0.73 0.56 0.69 0.7 0.73 0.76
Mitochondria 0.41 0.82 0.56 0.72 0.64 0.8 0.73 0.69 0.83 0.88 0.88 0.73 0.19 0.19 0.07 0.11 0.08 0.1 0 0.1 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.06 0.05 0 0.08 0.08 0.08 0.06 0 0.11 0 0 0 0 0 0 0 0 0.06 0.05 0 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.09 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.11 0.06 0.09 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 43 31 9 10 13 14 15 40 30 14 21 27 38 43 38 22 47 42 19 8 32 15 28 33
Bud 11 6 13 11 15 11 45 50 59 35 54 74 4 4 3 9 18 12 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 3 6 7 8 6 0 0 0 0 1 0
Cell Periphery 0 1 0 1 2 1 4 3 8 1 4 4 4 4 1 1 2 1 0 0 0 0 0 0
Cytoplasm 3 5 7 10 11 8 10 13 6 10 11 8 13 13 11 30 62 26 0 1 0 0 0 2
Endoplasmic Reticulum 10 16 9 3 7 22 20 16 15 11 10 14 32 26 28 11 12 13 0 0 0 1 3 2
Endosome 64 0 62 39 69 56 120 151 44 37 22 76 28 28 31 56 183 87 67 34 47 43 66 60
Golgi 224 42 168 81 142 39 55 53 35 15 21 26 108 116 150 74 215 186 296 99 253 221 398 454
Mitochondria 149 130 194 187 279 397 534 590 474 632 456 510 39 39 17 23 46 34 6 16 7 13 16 13
Nucleus 0 2 0 0 2 1 0 3 0 0 1 0 0 0 0 0 3 2 0 1 0 0 0 0
Nuclear Periphery 0 0 5 1 1 3 5 8 3 9 3 3 2 3 0 1 1 1 0 0 0 0 1 0
Nucleolus 3 0 4 3 7 6 32 49 14 26 11 14 2 2 3 0 11 5 0 0 0 0 0 0
Peroxisomes 3 0 9 15 22 17 57 65 43 42 22 79 2 3 0 1 24 6 12 4 22 17 23 27
SpindlePole 4 0 3 1 7 5 16 28 23 21 14 30 6 6 7 15 50 16 0 1 0 1 1 0
Vac/Vac Membrane 24 7 5 3 10 12 13 11 3 5 3 15 20 20 27 13 52 30 2 7 1 0 1 2
Unique Cell Count 361 158 344 259 435 497 733 854 570 717 520 694 201 203 236 209 566 345 407 178 368 316 545 601
Labelled Cell Count 538 240 488 365 587 593 927 1083 763 865 661 886 298 307 316 256 727 461 407 178 368 316 545 601


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 8.1 10.7 10.0 9.6 8.4 8.1 8.1 7.8 7.0 7.5 7.5 9.9 9.9 10.9 8.1 9.2 11.0 11.8 12.1 12.5
Std Deviation (1e-4) 1.4 1.4 1.8 1.5 1.6 1.5 1.5 1.7 1.7 1.5 1.7 1.6 1.4 1.4 1.2 1.4 1.7 2.1 1.5 1.7 1.8
Intensity Change (Log2) -0.09 -0.15 -0.34 -0.39 -0.4 -0.45 -0.61 -0.52 -0.5 -0.1 -0.1 0.03 -0.4 -0.22 0.05 0.15 0.18 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.9 0.3 0.2 -0.6 1.6 1.9 -0.7 1.1 1.1 6.5 7.2 5.8 3.9 3.5 4.8
Bud 0.3 -0.2 -1.3 1.6 1.5 3.6 0.8 3.6 3.8 -1.2 -1.2 -1.8 0.3 -0.5 -0.2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 0.5 -0.5 -0.8 -0.6 0 -0.8 0.1 0 2.7 2.6 1.8 5.6 4.9 3.4
Endoplasmic Reticulum -1.3 -1.0 1.4 0.1 -0.8 0 -1.2 -0.7 -0.6 5.7 4.7 4.5 1.6 -0.5 0.9
Endosome -1.0 -0.8 -2.8 -0.7 -0.1 -4.7 -6.7 -6.7 -3.2 -1.2 -1.3 -1.6 2.4 4.7 2.3
Golgi -4.3 -4.6 -13.6 -15.6 -17.2 -15.0 -18.9 -15.6 -17.5 1.1 1.9 3.5 -3.1 -3.2 1.3
Mitochondria 4.0 2.2 7.3 5.4 4.2 8.8 11.7 10.4 5.5 -8.4 -8.5 -12.1 -10.6 -16.0 -13.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.7 3.5 1.4 2.3 1.0 1.0 0 0 0 0 0.9 0
Peroxisomes 2.0 1.7 0.7 3.3 3.2 3.1 2.3 1.2 4.8 0 0 0 0 1.3 -0.8
SpindlePole 0 0 0 1.5 2.4 2.8 2.1 1.9 3.0 0 0 0 4.1 5.0 3.0
Vacuole 0 0.9 1.0 0.4 0 0 0 0 0.8 4.6 4.5 5.2 3.1 4.7 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8592 5.9309 5.5265 4.9572 5.0063 5.4714 3.7435 5.7568 5.1423 5.1502 4.9211 5.2809 3.7772 5.2678 4.5532 4.2358 3.8402 4.406
Actin 0.0411 0.018 0.025 0.0013 0.0068 0.0239 0.1025 0.0179 0.0141 0.0024 0.0173 0.0221 0.0216 0.0412 0.0368 0.0151 0.0084 0.0494
Bud 0.0002 0.0005 0.0021 0.0001 0 0.0002 0.0008 0.0004 0.0002 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0006 0.0003 0.0002
Bud Neck 0.0005 0.0005 0.0008 0.0002 0.0002 0.0008 0.004 0.0062 0.0003 0.0001 0.0003 0.0008 0.0005 0.0005 0.0004 0.0004 0.0005 0.0019
Bud Periphery 0.0001 0.0002 0.0007 0.0001 0 0.0001 0.001 0.0004 0.0002 0 0 0.0001 0.0002 0.0001 0.0001 0.0006 0.0001 0.0003
Bud Site 0.0011 0.0042 0.0013 0.0001 0.0001 0.0004 0.0219 0.0017 0.0003 0.0002 0.0001 0.0004 0.0006 0.005 0.0004 0.0078 0.001 0.0004
Cell Periphery 0.0001 0 0.0001 0 0 0 0.0006 0.0003 0 0 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001
Cytoplasm 0.0078 0.0065 0.0028 0.0016 0.0024 0.0018 0.0033 0.0037 0.0034 0.0018 0.0017 0.0012 0.0106 0.0091 0.0092 0.0016 0.0069 0.0017
Cytoplasmic Foci 0.1285 0.1394 0.1166 0.0967 0.1012 0.0913 0.1271 0.1293 0.1152 0.1136 0.0761 0.0736 0.1049 0.1378 0.1075 0.08 0.1913 0.0902
Eisosomes 0.0001 0 0 0 0 0 0.0002 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0.0002 0 0.0001
Endoplasmic Reticulum 0.0026 0.0007 0.0004 0.0003 0.0003 0.0003 0.0014 0.0006 0.0022 0.0003 0.0005 0.0005 0.0055 0.0005 0.0013 0.0005 0.0015 0.0008
Endosome 0.2288 0.2404 0.2063 0.3439 0.236 0.1775 0.1962 0.2397 0.2504 0.2945 0.1656 0.187 0.2496 0.2085 0.2124 0.3027 0.2741 0.1676
Golgi 0.5209 0.5723 0.6178 0.5415 0.5817 0.6653 0.3886 0.5815 0.5698 0.5348 0.7331 0.6723 0.4093 0.5618 0.5776 0.4688 0.4596 0.6251
Lipid Particles 0.0169 0.0025 0.0038 0.0022 0.0054 0.0089 0.0519 0.0057 0.0081 0.0083 0.0013 0.0117 0.0424 0.0032 0.0039 0.0068 0.0219 0.0055
Mitochondria 0.0102 0.0044 0.0053 0.006 0.0027 0.0101 0.0154 0.0042 0.0087 0.0069 0.002 0.0083 0.0069 0.004 0.0091 0.034 0.0023 0.0245
None 0.0044 0.0001 0.0002 0 0.0047 0.0003 0.0018 0.0001 0.0008 0.0001 0 0 0.0157 0.0001 0.0117 0.0002 0.0042 0.0001
Nuclear Periphery 0.0012 0.0003 0.0001 0.0001 0.0069 0.0001 0.0137 0.0003 0.0016 0.0001 0 0.0001 0.0303 0.0002 0.0013 0.0001 0.0006 0.0002
Nucleolus 0.0004 0 0.0001 0 0.0007 0.0001 0.0012 0.0001 0 0 0 0 0.0007 0 0.0002 0.0002 0.0001 0
Nucleus 0.0005 0.0001 0.0001 0 0.0023 0.0001 0.0052 0.0001 0.0001 0 0 0 0.0093 0.0001 0.0009 0.0002 0.0001 0.0001
Peroxisomes 0.0284 0.0076 0.0143 0.0014 0.0038 0.0159 0.0512 0.0043 0.0183 0.0328 0.001 0.0188 0.0623 0.0212 0.0236 0.0755 0.0218 0.029
Punctate Nuclear 0.0018 0.0001 0.0001 0 0.0427 0.0013 0.0027 0.0003 0.0003 0.0004 0 0.0001 0.003 0.0001 0.0016 0.0002 0.0029 0.0002
Vacuole 0.0027 0.0012 0.0009 0.0017 0.0009 0.0006 0.0045 0.0019 0.0027 0.0017 0.0002 0.0009 0.0125 0.0033 0.0009 0.0021 0.0008 0.0009
Vacuole Periphery 0.0017 0.0011 0.001 0.0025 0.0012 0.001 0.005 0.0013 0.0033 0.0019 0.0006 0.0017 0.0135 0.0029 0.001 0.0021 0.0014 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.1574 31.3639 30.5953 38.1968 33.7336 27.9737 31.8901 34.7505 35.3375 38.1023
Translational Efficiency 0.9632 0.6973 0.7972 0.857 0.7211 0.907 0.725 0.5484 0.7406 0.7208

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Delta adaptin-like subunit of the clathrin associated protein complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; suppressor of loss of casein kinase 1 function; the clathrin associated protein complex is also known as AP-3
Localization
Cell Percentages
Cell Cycle Regulation No
Subcompartmental Group N/A

Apl5

Apl5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apl5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available