ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; predicted to be palmitoylated |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 8 | 17 | 11 | 10 | 10 | 9 |
Bud | 1 | 0 | 0 | 0 | 0 | 1 | 1 | – | 4 | 3 | 7 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 3 | 3 | 8 | 14 |
Bud Neck | 0 | 3 | 20 | 10 | 18 | 8 | 15 | – | 11 | 18 | 11 | 11 | 1 | 1 | 1 | 5 | 3 | 2 | 4 | 7 | 3 | 5 | 10 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 7 | 10 | 4 | 3 | 1 | 8 | – | 2 | 0 | 4 | 2 | 11 | 11 | 5 | 14 | 24 | 16 | 7 | 21 | 15 | 21 | 12 | 16 |
Cytoplasm | 117 | 376 | 334 | 275 | 350 | 303 | 374 | – | 319 | 334 | 322 | 307 | 349 | 387 | 366 | 233 | 184 | 171 | 95 | 272 | 200 | 208 | 258 | 246 |
Endoplasmic Reticulum | 1 | 1 | 2 | 0 | 2 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 15 | 10 | 15 | 4 | 3 | 3 | 3 | 3 | 3 |
Endosome | 0 | 1 | 1 | 0 | 0 | 0 | 2 | – | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 2 | 1 | 1 | 3 | 2 | 2 | 3 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | 3 | 3 | 1 | 0 | 0 | 0 | 2 | 2 |
Mitochondria | 5 | 5 | 0 | 0 | 0 | 37 | 12 | – | 70 | 67 | 120 | 80 | 0 | 0 | 0 | 4 | 3 | 3 | 12 | 21 | 38 | 20 | 19 | 9 |
Nucleus | 1 | 0 | 0 | 1 | 0 | 4 | 3 | – | 3 | 1 | 2 | 1 | 0 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 1 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 3 | 5 | 3 | 14 | – | 11 | 15 | 14 | 18 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 1 | 0 | 0 | 3 | 3 |
Vac/Vac Membrane | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 4 | 5 | 1 | 2 | 0 | 0 | 0 | 1 | 2 | 4 | 1 | 4 | 0 | 2 | 3 | 8 |
Unique Cell Count | 124 | 382 | 339 | 278 | 357 | 318 | 391 | 373 | 390 | 420 | 377 | 351 | 391 | 370 | 243 | 198 | 184 | 148 | 372 | 292 | 291 | 346 | 337 | |
Labelled Cell Count | 126 | 393 | 367 | 294 | 379 | 358 | 430 | 425 | 444 | 487 | 431 | 363 | 406 | 378 | 280 | 234 | 218 | 148 | 372 | 292 | 291 | 346 | 337 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 3.9 | 4.6 | 5.0 | 5.0 | 3.4 | 4.2 | – | 3.6 | 3.8 | 3.8 | 3.6 | 4.9 | 5.2 | 5.3 | 5.6 | 6.2 | 6.3 | 4.0 | 4.4 | 4.5 |
Std Deviation (1e-4) | 1.4 | 0.6 | 1.1 | 5.7 | 1.7 | 1.0 | 1.7 | – | 1.4 | 1.3 | 1.3 | 0.8 | 1.2 | 1.7 | 2.1 | 1.6 | 1.9 | 2.8 | 1.5 | 1.9 | 1.9 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.12 | -0.43 | -0.13 | – | -0.35 | -0.27 | -0.29 | -0.35 | 0.09 | 0.17 | 0.19 | 0.28 | 0.43 | 0.44 | -0.2 | -0.05 | -0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.7919 | -0.5968 | -0.3927 | -0.1542 | -0.342 | -0.6751 | 0.9596 | 0.9496 | 1.361 | 0.98 | 1.0942 | 0.927 | 3.0128 | 3.8162 | 3.208 | 2.9096 | 3.929 | 3.3816 |
Actin | 0.03 | 0.0131 | 0.0342 | 0.0213 | 0.0094 | 0.033 | 0.0256 | 0.0017 | 0.0075 | 0.0082 | 0.0161 | 0.0141 | 0.0064 | 0.0017 | 0.0022 | 0.0017 | 0.0039 | 0.0047 |
Bud | 0.0027 | 0.006 | 0.0013 | 0.0033 | 0.0012 | 0.0027 | 0.001 | 0.0194 | 0.0012 | 0.0007 | 0.0006 | 0.0004 | 0.0003 | 0.0007 | 0.0015 | 0.0004 | 0.0006 | 0.001 |
Bud Neck | 0.0041 | 0.0082 | 0.0176 | 0.0087 | 0.0131 | 0.0331 | 0.0009 | 0.0023 | 0.0023 | 0.0022 | 0.002 | 0.019 | 0.0059 | 0.0042 | 0.005 | 0.0178 | 0.0069 | 0.0156 |
Bud Periphery | 0.0079 | 0.0085 | 0.0032 | 0.0078 | 0.006 | 0.012 | 0.0062 | 0.0069 | 0.0021 | 0.0024 | 0.0014 | 0.0031 | 0.0019 | 0.0024 | 0.0059 | 0.0025 | 0.007 | 0.0082 |
Bud Site | 0.0097 | 0.1009 | 0.0314 | 0.0221 | 0.0089 | 0.0033 | 0.0058 | 0.0521 | 0.0239 | 0.01 | 0.002 | 0.0012 | 0.0153 | 0.0757 | 0.0407 | 0.0054 | 0.0045 | 0.0018 |
Cell Periphery | 0.0523 | 0.0446 | 0.0427 | 0.0601 | 0.0261 | 0.0286 | 0.0723 | 0.0579 | 0.0485 | 0.0343 | 0.0365 | 0.0549 | 0.1221 | 0.0668 | 0.0848 | 0.0822 | 0.0424 | 0.0584 |
Cytoplasm | 0.3534 | 0.3743 | 0.4269 | 0.353 | 0.4835 | 0.4761 | 0.4589 | 0.5234 | 0.4504 | 0.5536 | 0.4088 | 0.5492 | 0.4943 | 0.5098 | 0.4912 | 0.6413 | 0.4535 | 0.5551 |
Cytoplasmic Foci | 0.0285 | 0.0091 | 0.0149 | 0.0179 | 0.0238 | 0.0175 | 0.0282 | 0.0042 | 0.0204 | 0.0144 | 0.0145 | 0.008 | 0.0136 | 0.0036 | 0.0027 | 0.0021 | 0.0047 | 0.0035 |
Eisosomes | 0.0124 | 0.0074 | 0.0029 | 0.0013 | 0.0017 | 0.0014 | 0.0014 | 0.0013 | 0.0009 | 0.0034 | 0.008 | 0.0006 | 0.0044 | 0.0015 | 0.0015 | 0.0006 | 0.0014 | 0.0006 |
Endoplasmic Reticulum | 0.0072 | 0.0038 | 0.0079 | 0.0109 | 0.0047 | 0.0042 | 0.0115 | 0.0061 | 0.0026 | 0.0047 | 0.0066 | 0.0019 | 0.0054 | 0.006 | 0.0038 | 0.0037 | 0.0055 | 0.0024 |
Endosome | 0.0189 | 0.0005 | 0.0126 | 0.0076 | 0.041 | 0.0031 | 0.0415 | 0.0011 | 0.0268 | 0.007 | 0.0132 | 0.0037 | 0.0043 | 0.0014 | 0.0004 | 0.0002 | 0.0005 | 0.0007 |
Golgi | 0.0065 | 0.0005 | 0.0039 | 0.0076 | 0.017 | 0.0033 | 0.0153 | 0.0002 | 0.0096 | 0.0055 | 0.0026 | 0.0171 | 0.0012 | 0.0004 | 0.0004 | 0.0003 | 0.0005 | 0.0003 |
Lipid Particles | 0.0109 | 0.0015 | 0.0043 | 0.0165 | 0.0781 | 0.0036 | 0.0078 | 0.0012 | 0.0039 | 0.024 | 0.0046 | 0.0109 | 0.0032 | 0.0015 | 0.0009 | 0.0005 | 0.0009 | 0.0006 |
Mitochondria | 0.0085 | 0.0003 | 0.0018 | 0.0033 | 0.0182 | 0.005 | 0.0048 | 0.0009 | 0.0028 | 0.0024 | 0.0019 | 0.0015 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 |
None | 0.422 | 0.401 | 0.3642 | 0.3768 | 0.1482 | 0.3535 | 0.2857 | 0.3152 | 0.3448 | 0.3127 | 0.4397 | 0.2986 | 0.3132 | 0.3184 | 0.3538 | 0.2391 | 0.4622 | 0.3428 |
Nuclear Periphery | 0.0064 | 0.0003 | 0.0021 | 0.0033 | 0.0014 | 0.0007 | 0.0144 | 0.0003 | 0.0084 | 0.0015 | 0.0142 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0003 |
Nucleolus | 0.0011 | 0.0055 | 0.0064 | 0.0016 | 0.0398 | 0.0099 | 0.0006 | 0.0005 | 0.0143 | 0.0023 | 0.0021 | 0.0001 | 0.0009 | 0.0005 | 0.0004 | 0.0001 | 0.0004 | 0.0003 |
Nucleus | 0.0032 | 0.0106 | 0.0061 | 0.0478 | 0.0576 | 0.0046 | 0.0037 | 0.0012 | 0.0055 | 0.0011 | 0.0057 | 0.0003 | 0.0006 | 0.0011 | 0.0007 | 0.0008 | 0.001 | 0.0006 |
Peroxisomes | 0.0042 | 0.0003 | 0.0044 | 0.0203 | 0.0091 | 0.0014 | 0.0084 | 0.0001 | 0.008 | 0.0049 | 0.0009 | 0.0136 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 |
Punctate Nuclear | 0.0046 | 0.001 | 0.0021 | 0.0029 | 0.0044 | 0.0007 | 0.0018 | 0.0006 | 0.0084 | 0.0017 | 0.0159 | 0.0002 | 0.0008 | 0.0006 | 0.0003 | 0.0002 | 0.0004 | 0.0006 |
Vacuole | 0.0045 | 0.0024 | 0.0082 | 0.0054 | 0.0058 | 0.0021 | 0.0031 | 0.0032 | 0.0059 | 0.0026 | 0.0019 | 0.0014 | 0.0048 | 0.003 | 0.0032 | 0.0011 | 0.003 | 0.002 |
Vacuole Periphery | 0.0012 | 0.0001 | 0.0006 | 0.0005 | 0.0009 | 0.0003 | 0.0011 | 0.0001 | 0.0018 | 0.0004 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.7076 | 36.8356 | 53.6549 | 53.9531 | 46.4998 | 19.8289 | 37.9342 | 47.0164 | 43.9816 | 51.6035 |
Translational Efficiency | 2.44 | 2.1731 | 1.628 | 1.165 | 1.5962 | 3.3402 | 1.8522 | 1.4033 | 1.7008 | 1.6795 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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