Standard name
Human Ortholog
Description cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0.05 0.1 0.12 0.13 0.18 0.11 0.1 0.11 0.12 0.12 0.1 0.1 0.17 0.22 0.08 0.14 0.16 0 0 0 0.09 0.08 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.22 0.29 0.19 0.45 0.45 0.45 0.49 0.48 0.54 0.61 0 0 0 0.06 0 0 0 0 0 0 0 0
Nucleus 0.82 0.78 0.72 0.65 0.57 0.45 0.43 0.44 0.45 0.42 0.47 0.41 0.71 0.58 0.54 0.68 0.61 0.62 0.83 0.82 0.75 0.59 0.55 0.54
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.21 0.08 0.07 0.22 0.17 0.18 0.17 0.18 0.15 0.12 0.1 0.23 0.28 0.25 0.22 0.25 0.24 0.06 0.06 0.11 0.19 0.2 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 1 1
Bud 0 1 0 2 4 11 12 11 11 19 9 17 1 1 0 0 0 0 6 7 4 13 27 25
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 5 3
Bud Site 0 0 0 1 0 3 7 5 11 13 15 20 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 1 1 1 1 0 1 1 0 0 0 0 0 0 0 0
Cytoplasm 16 20 41 41 66 110 89 80 75 90 70 96 47 101 125 7 18 16 9 13 19 36 41 18
Endoplasmic Reticulum 0 1 0 1 0 0 0 2 0 0 0 1 2 1 1 2 4 1 0 0 0 1 4 5
Endosome 1 4 4 3 20 17 3 5 4 25 0 1 6 25 7 3 1 0 4 4 7 5 7 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 0 0 1 2 0 1 3
Mitochondria 6 13 94 102 98 283 377 356 327 361 322 597 3 3 3 5 5 1 2 2 4 4 6 15
Nucleus 256 315 305 229 296 282 363 348 301 319 279 402 338 340 306 58 77 61 307 356 354 244 301 277
Nuclear Periphery 0 0 0 3 1 0 3 2 4 2 2 2 0 0 0 0 0 0 0 0 1 1 1 2
Nucleolus 0 0 0 0 1 3 31 61 16 17 13 34 0 5 2 0 2 1 5 9 3 9 12 19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 1 0 0 1 1 0 0 0 0 0 1 0 0 0 8 8 10 8 22 12
Vac/Vac Membrane 60 83 35 23 115 104 154 132 119 116 69 103 111 165 143 19 31 24 23 27 50 78 109 107
Unique Cell Count 313 403 422 352 515 626 840 791 666 756 591 984 473 591 566 85 126 99 372 436 472 413 550 511
Labelled Cell Count 340 437 479 406 602 813 1039 1003 871 963 780 1274 510 642 591 95 139 104 372 436 472 413 550 511


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 6.6 4.7 4.7 5.2 5.0 5.0 5.2 5.3 5.0 5.1 5.1 7.7 7.9 8.7 10.6 10.3 10.5 7.5 7.5 8.0
Std Deviation (1e-4) 1.5 0.9 0.8 1.1 1.2 1.0 1.2 1.4 1.3 1.2 1.3 1.2 1.5 1.6 1.8 1.9 2.0 2.1 1.7 1.8 1.8
Intensity Change (Log2) – – – -0.01 0.13 0.09 0.09 0.16 0.18 0.1 0.11 0.13 0.72 0.74 0.89 1.17 1.13 1.16 0.68 0.68 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3.3 0 2.7 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 3.3 2.9 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 1.5 3.6 0.5 0.2 0.8 1.1 1.1 0 0.1 3.3 5.1 -0.4 1.5 1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.8 2.0 0 0 0 2.5 0 0 0 3.1 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -1.2 7.6 7.8 7.8 8.9 8.6 10.3 13.2 -10.4 -11.6 -11.4 -3.5 -4.7 -4.9
Nucleus -2.2 -4.7 -8.7 -9.8 -9.4 -8.8 -9.9 -8.0 -10.8 -0.3 -4.8 -5.8 -0.8 -2.4 -2.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 4.0 5.9 3.2 3.1 3.1 3.9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.9 5.8 3.9 4.7 4.0 4.4 3.5 1.7 1.3 6.1 7.7 6.9 3.8 4.9 4.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8886 2.09 1.9068 1.5563 1.3688 1.8386 4.5575 5.1709 4.618 3.906 4.1145 4.6854 2.0751 2.7491 2.2162 2.2925 2.1062 2.3305
Actin 0.0024 0.0001 0.0061 0.014 0.0072 0.0007 0.0043 0.0014 0.0099 0.0007 0.0047 0.0048 0.0002 0 0 0 0 0
Bud 0.0007 0.0002 0.0011 0.0002 0.0003 0.0014 0.0004 0.0003 0.0007 0.0001 0.0001 0.0001 0 0 0 0 0 0
Bud Neck 0.006 0.0006 0.0012 0.0003 0.001 0.0017 0.0058 0.0004 0.0009 0.0065 0.0007 0.0017 0.0033 0.0002 0.0001 0.0001 0.0002 0.0009
Bud Periphery 0.001 0.0001 0.0007 0.0006 0.0002 0.0005 0.0016 0.0003 0.0018 0.0001 0.0002 0.0001 0 0 0 0 0 0
Bud Site 0.0095 0.0021 0.0028 0.0007 0.0005 0.0002 0.0018 0.0017 0.0012 0.003 0.0004 0.0001 0.0003 0.0001 0 0 0 0
Cell Periphery 0.0006 0 0.0001 0.0001 0.0003 0 0.0004 0 0.0002 0.0001 0 0 0 0 0 0 0 0
Cytoplasm 0.0373 0.0466 0.0254 0.0072 0.0137 0.0189 0.0289 0.0355 0.0142 0.01 0.0045 0.0155 0.0217 0.0413 0.0047 0.0034 0.0014 0.0128
Cytoplasmic Foci 0.0148 0.0005 0.0079 0.006 0.0179 0.0008 0.0025 0.0014 0.0036 0.0179 0.0158 0.0008 0.0025 0.0001 0 0 0 0.0002
Eisosomes 0.0001 0 0.0001 0.0004 0.0006 0 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.004 0.0008 0.0019 0.0006 0.0023 0.0004 0.0026 0.001 0.0015 0.0003 0.0012 0.0007 0.0007 0.0008 0.0001 0 0 0.0002
Endosome 0.024 0.0009 0.0113 0.01 0.0068 0.0016 0.0098 0.0022 0.0076 0.0041 0.0255 0.003 0.0042 0.0004 0.0001 0 0 0.0005
Golgi 0.0069 0 0.0044 0.008 0.0015 0.0007 0.0021 0.0003 0.0019 0.0011 0.003 0.0017 0.0003 0 0 0 0 0
Lipid Particles 0.0091 0.0001 0.005 0.0034 0.008 0.0011 0.0061 0.0001 0.0026 0.0056 0.0044 0.0005 0.0009 0 0 0 0 0
Mitochondria 0.007 0.0002 0.0047 0.018 0.0055 0.0018 0.0029 0.001 0.011 0.0004 0.0041 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001
None 0.017 0.0113 0.0108 0.0025 0.0222 0.0012 0.0047 0.0008 0.0113 0.0011 0.0032 0.0131 0.0082 0.0011 0.0002 0.0001 0 0.0042
Nuclear Periphery 0.021 0.0147 0.0067 0.0032 0.0154 0.0061 0.0112 0.0085 0.0076 0.0018 0.0086 0.0029 0.0058 0.0151 0.0024 0.002 0.002 0.0035
Nucleolus 0.0083 0.0025 0.0025 0.0028 0.0126 0.0076 0.0031 0.0025 0.0043 0.0062 0.0109 0.0044 0.0024 0.0015 0.0014 0.0012 0.0022 0.0033
Nucleus 0.7841 0.9055 0.8883 0.9131 0.8629 0.9291 0.8958 0.9354 0.9028 0.9051 0.882 0.942 0.9406 0.9356 0.9874 0.9899 0.9925 0.9678
Peroxisomes 0.0121 0 0.0059 0.0043 0.003 0.0003 0.0026 0.0006 0.0026 0.0276 0.0179 0.0001 0.0002 0 0 0 0 0
Punctate Nuclear 0.0248 0.0121 0.0104 0.0029 0.0147 0.0245 0.0073 0.0052 0.0085 0.0025 0.0099 0.0078 0.0079 0.0031 0.0035 0.003 0.0015 0.006
Vacuole 0.0075 0.0014 0.0024 0.0009 0.0022 0.0008 0.0031 0.0009 0.0047 0.0055 0.0019 0.0007 0.0005 0.0003 0.0001 0 0.0001 0.0002
Vacuole Periphery 0.0017 0.0003 0.0004 0.0007 0.0013 0.0004 0.0028 0.0002 0.0011 0.0001 0.0011 0.0001 0.0001 0.0001 0 0 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9977 6.9038 10.2458 18.2998 16.897 15.4372 15.2696 17.0784 21.6722 16.9367
Translational Efficiency 0.9302 2.0515 1.1148 0.868 0.8303 1.385 1.291 1.2377 1.4057 1.1292

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (81%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Tpk2

Tpk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tpk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available