Standard name | |
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Human Ortholog | |
Description | Conserved casein kinase; regulates diverse events including: vesicular traffic, DNA repair, the CVT pathway, monopolar attachment of sister kinetochores at meiosis I, and ribosomal subunit biogenesis; monopolin subunit; binds the RNAPII CTD; phosphorylates COPII coat subunits; interacts with Sit4p phosphatase; antagonizes calcineurin signaling, reducing nuclear accumulation of Crz1p; phosphorylates Dsn1p, the kinetochore receptor for monopolin; homolog of mammalian CK1delta |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 1 | 4 | 7 | 10 | 8 | 13 | 11 | 23 | 7 | 21 | 5 | 2 | 0 | 6 | 3 | 3 | 4 | 2 | 1 | 1 | 4 | 3 |
Bud | 0 | 0 | 0 | 0 | 8 | 8 | 18 | 10 | 15 | 13 | 20 | 19 | 2 | 3 | 0 | 3 | 1 | 1 | 10 | 7 | 3 | 5 | 19 | 30 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 1 | 4 | 2 | 7 | 7 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 9 | 6 | 9 | 16 | 0 | 2 | 5 | 3 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 104 | 12 | 44 | 31 | 71 | 126 | 191 | 156 | 113 | 153 | 120 | 145 | 100 | 151 | 161 | 105 | 86 | 98 | 78 | 25 | 52 | 34 | 76 | 136 |
Endoplasmic Reticulum | 15 | 3 | 3 | 2 | 15 | 47 | 49 | 40 | 17 | 55 | 27 | 40 | 23 | 12 | 30 | 1 | 5 | 5 | 11 | 1 | 3 | 4 | 7 | 17 |
Endosome | 1 | 0 | 3 | 5 | 15 | 9 | 46 | 30 | 23 | 19 | 10 | 9 | 2 | 1 | 6 | 3 | 9 | 3 | 15 | 3 | 4 | 1 | 15 | 16 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 2 | 2 | 11 | 5 | 14 | 15 | 25 | 12 | 23 | 27 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 4 |
Nucleus | 14 | 0 | 4 | 15 | 13 | 8 | 7 | 3 | 16 | 7 | 16 | 12 | 13 | 19 | 19 | 8 | 1 | 3 | 26 | 15 | 18 | 5 | 31 | 40 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 1 | 0 | 0 | 3 | 7 |
Nucleolus | 0 | 0 | 4 | 0 | 4 | 4 | 17 | 8 | 3 | 4 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 8 | 0 | 6 | 0 | 3 | 3 |
Vac/Vac Membrane | 27 | 1 | 11 | 42 | 61 | 12 | 68 | 50 | 55 | 40 | 36 | 19 | 67 | 68 | 70 | 15 | 39 | 19 | 14 | 6 | 4 | 8 | 37 | 51 |
Unique Cell Count | 146 | 16 | 65 | 92 | 188 | 212 | 379 | 310 | 256 | 303 | 248 | 271 | 191 | 241 | 270 | 133 | 132 | 126 | 192 | 68 | 107 | 69 | 214 | 332 |
Labelled Cell Count | 164 | 16 | 72 | 101 | 206 | 230 | 421 | 331 | 290 | 335 | 274 | 318 | 212 | 258 | 292 | 146 | 151 | 136 | 192 | 68 | 107 | 69 | 214 | 332 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.6 | 12.9 | 9.1 | 9.6 | 9.2 | 8.6 | 8.6 | 8.9 | 9.1 | 9.3 | 9.2 | 9.5 | 11.9 | 11.0 | 12.1 | 11.5 | 12.1 | 12.4 | – | 14.7 | 15.5 |
Std Deviation (1e-4) | 2.1 | 1.6 | 1.6 | 1.6 | 1.7 | 1.3 | 1.4 | 1.5 | 1.7 | 1.8 | 1.8 | 1.8 | 1.4 | 1.7 | 1.6 | 2.1 | 2.0 | 2.2 | – | 2.4 | 2.8 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.02 | -0.07 | -0.07 | -0.03 | 0.0 | 0.04 | 0.03 | 0.08 | 0.4 | 0.27 | 0.41 | 0.34 | 0.42 | 0.45 | – | 0.7 | 0.78 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -4.2 | -4.2 | -1.2 | -2.6 | -2.6 | -3.4 | -2.5 | -2.8 | -2.1 | -2.2 | -0.7 | -1.2 | 1.7 | -0.4 | 1.5 |
Endoplasmic Reticulum | 0 | 0 | 3.2 | 1.9 | 1.9 | 0 | 2.7 | 1.5 | 2.2 | 1.7 | 0 | 1.6 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1.8 | 1.3 | 1.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 1.6 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 3.7 | 2.4 | -2.9 | 0.2 | -0.2 | 0.8 | -0.8 | -0.5 | -2.5 | 2.7 | 1.8 | 1.5 | -1.1 | 1.9 | -0.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.023 | 0.006 | 0.0382 | 0.0112 | 0.0096 | 0.0181 | 0.0256 | 0.0068 | 0.0683 | 0.0297 | 0.0118 | 0.0152 | 0.1744 | 0.0651 | 0.1607 | 0.1306 | 0.0004 | 0.0448 |
Bud | 0.0007 | 0.0021 | 0.0036 | 0.0013 | 0.0179 | 0.0011 | 0.0007 | 0.0011 | 0.0013 | 0.0007 | 0.003 | 0.0003 | 0.0028 | 0.0014 | 0.0011 | 0.0048 | 0.0001 | 0.0006 |
Bud Neck | 0.0217 | 0.0063 | 0.0387 | 0.0131 | 0.0138 | 0.2562 | 0.0502 | 0.0127 | 0.0344 | 0.0203 | 0.0048 | 0.2709 | 0.0385 | 0.007 | 0.0524 | 0.0108 | 0.0029 | 0.4576 |
Bud Periphery | 0.0007 | 0.0007 | 0.0013 | 0.0014 | 0.0029 | 0.0016 | 0.0005 | 0.0005 | 0.0008 | 0.0004 | 0.0045 | 0.0003 | 0.0015 | 0.0004 | 0.0003 | 0.006 | 0 | 0.0004 |
Bud Site | 0.0037 | 0.0113 | 0.0077 | 0.0027 | 0.0055 | 0.0032 | 0.0119 | 0.0162 | 0.007 | 0.0019 | 0.0012 | 0.0003 | 0.0195 | 0.0523 | 0.0064 | 0.0028 | 0 | 0.0006 |
Cell Periphery | 0.0004 | 0.0003 | 0.0006 | 0.0004 | 0.0006 | 0.0003 | 0.0008 | 0.0008 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0.0013 | 0.0006 | 0.0004 | 0.0013 | 0 | 0.0002 |
Cytoplasm | 0.6555 | 0.6373 | 0.5936 | 0.687 | 0.7129 | 0.4471 | 0.729 | 0.6982 | 0.6748 | 0.6363 | 0.7234 | 0.4934 | 0.6143 | 0.7874 | 0.6803 | 0.4558 | 0.9625 | 0.4005 |
Cytoplasmic Foci | 0.0103 | 0.0106 | 0.0128 | 0.0061 | 0.0165 | 0.0051 | 0.0108 | 0.0077 | 0.0127 | 0.03 | 0.033 | 0.0025 | 0.0517 | 0.0282 | 0.017 | 0.0129 | 0.0005 | 0.0174 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0007 | 0 | 0 |
Endoplasmic Reticulum | 0.0149 | 0.0074 | 0.0175 | 0.021 | 0.0297 | 0.0086 | 0.0161 | 0.0067 | 0.0163 | 0.0102 | 0.0326 | 0.009 | 0.0089 | 0.0032 | 0.009 | 0.0359 | 0.0007 | 0.0026 |
Endosome | 0.0082 | 0.0025 | 0.0055 | 0.004 | 0.0199 | 0.0034 | 0.0111 | 0.0063 | 0.0068 | 0.0151 | 0.0246 | 0.0019 | 0.0228 | 0.0018 | 0.002 | 0.1002 | 0.0001 | 0.0028 |
Golgi | 0.0013 | 0.0003 | 0.001 | 0.0004 | 0.0029 | 0.0007 | 0.0021 | 0.0014 | 0.0033 | 0.008 | 0.0041 | 0.0003 | 0.0159 | 0.001 | 0.0009 | 0.134 | 0 | 0.0124 |
Lipid Particles | 0.0048 | 0.0003 | 0.0012 | 0.001 | 0.0029 | 0.0006 | 0.0015 | 0.0004 | 0.0019 | 0.0025 | 0.0013 | 0.0002 | 0.002 | 0.0003 | 0.0003 | 0.001 | 0 | 0.0008 |
Mitochondria | 0.0008 | 0.0014 | 0.0007 | 0.0004 | 0.0145 | 0.0043 | 0.0013 | 0.0004 | 0.0017 | 0.001 | 0.002 | 0.0004 | 0.0021 | 0.0006 | 0.0004 | 0.0851 | 0 | 0.0074 |
None | 0.0017 | 0.0021 | 0.002 | 0.0021 | 0.004 | 0.001 | 0.0038 | 0.0013 | 0.0055 | 0.0025 | 0.0026 | 0.0011 | 0.0032 | 0.0028 | 0.002 | 0.002 | 0.0007 | 0.0005 |
Nuclear Periphery | 0.0249 | 0.0225 | 0.0275 | 0.0224 | 0.0297 | 0.0166 | 0.0248 | 0.0136 | 0.0205 | 0.0185 | 0.0245 | 0.0165 | 0.0129 | 0.0064 | 0.0149 | 0.0046 | 0.0011 | 0.0025 |
Nucleolus | 0.0005 | 0.0009 | 0.0009 | 0.0005 | 0.0008 | 0.0005 | 0.0004 | 0.0006 | 0.0006 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Nucleus | 0.2138 | 0.2666 | 0.2154 | 0.1941 | 0.0831 | 0.2169 | 0.0935 | 0.2015 | 0.116 | 0.1644 | 0.0913 | 0.1766 | 0.0142 | 0.02 | 0.0286 | 0.0024 | 0.03 | 0.0121 |
Peroxisomes | 0.0018 | 0.0007 | 0.001 | 0.0003 | 0.002 | 0.0006 | 0.0014 | 0.0004 | 0.0023 | 0.0022 | 0.0034 | 0.0003 | 0.0033 | 0.0044 | 0.0025 | 0.0005 | 0 | 0.0293 |
Punctate Nuclear | 0.0092 | 0.0189 | 0.0286 | 0.0289 | 0.023 | 0.0123 | 0.0124 | 0.0218 | 0.0236 | 0.0548 | 0.0273 | 0.0091 | 0.009 | 0.0164 | 0.0202 | 0.0016 | 0.0009 | 0.0069 |
Vacuole | 0.0018 | 0.0016 | 0.0018 | 0.0016 | 0.0063 | 0.0014 | 0.0016 | 0.0014 | 0.0014 | 0.0008 | 0.0033 | 0.001 | 0.001 | 0.0006 | 0.0003 | 0.0031 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0016 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0003 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0038 | 0 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 29.7213 | 23.2479 | 34.009 | 51.0676 | 44.7969 | 27.477 | 38.2811 | 40.7955 | 41.88 | 48.8261 |
Translational Efficiency | 1.4366 | 1.6119 | 1.0627 | 0.7229 | 0.8417 | 1.5198 | 0.8499 | 0.7098 | 0.8568 | 0.8033 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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