Standard name
Human Ortholog
Description Aurora kinase of chromosomal passenger complex; mediates release of mono-oriented kinetochores from microtubules in meiosis I, and kinetochore release from SPB clusters at meiotic exit; helps maintain condensed chromosomes during anaphase; required for SPB cohesion and prevention of multipolar spindle formation; promotes telomerase release at G2/M; Iocalizes to nuclear foci that diffuse upon DNA replication stress; required for inhibition of karyopherin Pse1p upon SAC arrest
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.07 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0.06 0.09 0.05 0 0.07 0.08 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.09 0.09 0 0.17 0.18 0.21 0.2 0.28 0.2 0.35 0.22 0.26 0.24 0.41 0.47 0.09 0.1 0.11 0 0 0 0.07 0.13 0.23
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.21 0 0 0.05 0 0 0 0 0 0 0 0.06 0.09 0.21 0.25 0.26 0.2 0.09 0.13 0.09 0.11 0.09 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.59 0.2 0.17 0.08 0.08 0.31 0.46 0.32 0.62 0.52 0.76 0.59 0 0 0 0.19 0.11 0.14 0.05 0.06 0.05 0.08 0.1 0.08
Nucleus 0.1 0.12 0.22 0.08 0.15 0.19 0.13 0.15 0.12 0.1 0.14 0.11 0.17 0.2 0.18 0.16 0.17 0.22 0.06 0.05 0.07 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.16 0 0.05 0.06 0.07 0.07 0 0.06 0 0 0 0.07 0 0 0.33 0.35 0.45 0.05 0.09 0.09 0.07 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.38 0.41 0.61 0.67 0.6 0.41 0.34 0.36 0.19 0.17 0.16 0.22 0.5 0.35 0.24 0.54 0.53 0.38 0.53 0.46 0.48 0.45 0.49 0.45
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0 0.06 0.05 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 3 3
Bud 2 1 1 4 3 6 10 16 8 13 4 9 0 1 0 0 0 0 0 0 0 1 4 6
Bud Neck 2 0 1 11 23 9 7 17 18 15 2 7 2 4 0 0 0 0 1 0 0 0 2 4
Bud Site 5 1 0 5 8 7 11 6 9 9 3 13 1 0 0 1 1 3 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 21 11 1 30 47 42 59 68 44 95 21 45 31 75 90 12 12 16 7 5 3 8 30 51
Endoplasmic Reticulum 5 1 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 2 0 0 0 1 1
Endosome 6 27 0 1 14 5 2 4 1 0 0 1 8 16 39 34 31 29 25 23 8 13 20 16
Golgi 0 1 0 1 0 0 0 0 0 0 0 0 0 1 2 0 2 3 2 1 0 0 1 0
Mitochondria 132 25 4 14 20 61 135 77 135 144 73 102 5 1 0 26 13 21 14 11 4 8 23 18
Nucleus 23 15 5 14 38 37 37 35 26 27 13 20 22 37 35 21 20 32 16 8 6 1 2 0
Nuclear Periphery 4 0 0 0 0 0 0 0 1 1 0 0 0 0 0 3 0 1 1 2 0 0 0 0
Nucleolus 25 20 1 8 15 13 20 9 13 12 3 5 9 6 3 45 41 65 13 16 8 8 7 1
Peroxisomes 1 2 0 0 0 0 1 1 0 0 0 1 0 0 2 2 3 4 0 0 0 0 1 0
SpindlePole 85 53 14 115 157 81 100 85 42 46 15 39 64 65 45 73 62 55 144 84 46 52 115 100
Vac/Vac Membrane 3 8 0 0 1 2 3 3 0 4 1 1 0 5 3 5 8 8 5 11 4 3 4 7
Unique Cell Count 223 128 23 172 262 199 292 239 218 275 96 174 127 184 190 135 117 145 274 186 97 118 235 224
Labelled Cell Count 314 166 29 204 327 263 385 321 297 366 135 243 142 211 219 224 194 237 274 186 97 118 235 224


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.2 4.4 4.1 4.5 4.8 3.9 3.5 4.2 3.1 3.4 2.9 3.4 4.5 5.2 5.3 6.5 6.7 6.5 4.5 4.8 5.2
Std Deviation (1e-4) 0.5 0.8 1.0 1.8 2.0 1.6 1.4 1.9 0.9 1.3 0.9 1.5 0.6 1.8 2.1 1.5 1.8 1.9 1.6 1.5 2.2
Intensity Change (Log2) – – – 0.12 0.24 -0.06 -0.23 0.04 -0.41 -0.29 -0.48 -0.26 0.13 0.34 0.36 0.66 0.71 0.67 0.14 0.24 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2667 0.6402 0.3112 0.2031 0.0504 0.253 -0.6142 0.0022 0.1116 0.0372 -0.0729 -0.0779 -0.9709 -0.636 -0.9695 -0.1758 -0.3766 -0.8763
Actin 0.005 0.0006 0.0002 0.0002 0.0043 0.0009 0.0215 0.0002 0.0022 0.0032 0.0043 0.0039 0.0289 0.0004 0.0026 0.0624 0.0171 0.0029
Bud 0.0002 0.0006 0 0.0001 0.0002 0.0004 0.0005 0.0002 0.0001 0.0005 0.0004 0.0002 0.0004 0.0025 0.0001 0.0007 0.0006 0.0001
Bud Neck 0.0027 0.0011 0.0018 0.001 0.0098 0.0096 0.002 0.0023 0.0012 0.0185 0.0064 0.0135 0.002 0.0013 0.001 0.0732 0.0045 0.0076
Bud Periphery 0.0005 0.0002 0 0 0.0012 0.0002 0.0008 0.0003 0.0001 0.0005 0.0005 0.0006 0.001 0.0005 0.0002 0.0013 0.0015 0.0005
Bud Site 0.0015 0.002 0.0003 0.0001 0.0008 0.0003 0.002 0.0026 0.0009 0.0186 0.0007 0.0007 0.0041 0.0048 0.0011 0.0053 0.0023 0.0005
Cell Periphery 0.0001 0.0001 0 0 0.0004 0.0001 0.0002 0.0001 0 0.0002 0.0001 0.0003 0.0002 0.0001 0 0.0003 0.0003 0.0002
Cytoplasm 0.0179 0.0059 0.0043 0.017 0.0073 0.0083 0.0317 0.017 0.0056 0.02 0.0163 0.0151 0.022 0.0136 0.0173 0.0102 0.0028 0.0066
Cytoplasmic Foci 0.0595 0.025 0.0048 0.0197 0.0052 0.0232 0.0769 0.027 0.0164 0.0242 0.0119 0.0208 0.0773 0.0401 0.016 0.0042 0.0233 0.0318
Eisosomes 0.0003 0.0001 0 0.0001 0.001 0.0001 0.0002 0.0001 0 0 0.0001 0.0002 0.0003 0 0 0.0004 0.0006 0.0001
Endoplasmic Reticulum 0.0011 0.0005 0.0001 0.0003 0.0013 0.0003 0.0031 0.0007 0.0009 0.0006 0.0024 0.0029 0.0033 0.0004 0.001 0.0015 0.0021 0.0005
Endosome 0.0088 0.0055 0.0008 0.0008 0.0054 0.0041 0.0328 0.0038 0.0054 0.0092 0.0317 0.011 0.0229 0.0034 0.0116 0.0103 0.033 0.0085
Golgi 0.0019 0.0008 0.0002 0.0001 0.0017 0.0014 0.0098 0.0008 0.0022 0.0037 0.0073 0.006 0.0058 0.0009 0.0013 0.0059 0.0092 0.0032
Lipid Particles 0.0274 0.013 0.0011 0.0021 0.0281 0.054 0.0416 0.0192 0.0109 0.013 0.0073 0.0394 0.0378 0.0056 0.0094 0.0081 0.0712 0.0717
Mitochondria 0.0063 0.0008 0.0003 0.0003 0.0283 0.0029 0.0058 0.0005 0.0014 0.0014 0.0044 0.0093 0.009 0.0003 0.0008 0.0039 0.0152 0.0144
None 0.152 0.0198 0.0565 0.0502 0.0472 0.0343 0.1075 0.1218 0.0226 0.0125 0.0718 0.0497 0.1149 0.0823 0.0505 0.0377 0.0624 0.0256
Nuclear Periphery 0.0153 0.0229 0.009 0.0105 0.1205 0.016 0.0249 0.0073 0.0075 0.0133 0.03 0.0108 0.0246 0.0073 0.0155 0.0096 0.0995 0.0185
Nucleolus 0.0382 0.0808 0.0446 0.0176 0.0752 0.0531 0.0237 0.0572 0.0512 0.0208 0.1128 0.0579 0.0118 0.0478 0.0302 0.0213 0.0855 0.0523
Nucleus 0.0848 0.2235 0.3824 0.4373 0.1503 0.0838 0.0638 0.2581 0.2883 0.3939 0.2559 0.1126 0.0605 0.2152 0.3571 0.392 0.0953 0.0806
Peroxisomes 0.0138 0.0132 0.0003 0.0005 0.004 0.0109 0.0102 0.0014 0.0138 0.0358 0.0014 0.0134 0.012 0.009 0.0078 0.0052 0.0236 0.0123
Punctate Nuclear 0.5604 0.5814 0.4929 0.4414 0.5021 0.6952 0.5353 0.4779 0.5685 0.4084 0.4303 0.6297 0.5545 0.5638 0.4735 0.3434 0.4395 0.66
Vacuole 0.0017 0.0017 0.0001 0.0005 0.0022 0.0006 0.0043 0.0013 0.0004 0.0013 0.0021 0.0014 0.0052 0.0007 0.0024 0.0022 0.0058 0.0013
Vacuole Periphery 0.0006 0.0006 0.0001 0.0001 0.0033 0.0005 0.0014 0.0002 0.0002 0.0002 0.0016 0.0006 0.0014 0.0001 0.0005 0.0007 0.0048 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9935 2.6804 2.6519 4.3998 7.7751 3.9956 7.227 2.7437 4.4557 7.7048
Translational Efficiency 1.1512 2.8725 1.2427 1.0432 1.2759 3.5506 0.8132 2.2102 1.2534 1.5017

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Aurora kinase of chromosomal passenger complex; mediates release of mono-oriented kinetochores from microtubules in meiosis I, and kinetochore release from SPB clusters at meiotic exit; helps maintain condensed chromosomes during anaphase; required for SPB cohesion and prevention of multipolar spindle formation; promotes telomerase release at G2/M; Iocalizes to nuclear foci that diffuse upon DNA replication stress; required for inhibition of karyopherin Pse1p upon SAC arrest
Localization
Cell Percentages nucleus (27%), nucleolus (15%), mixed (28%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ipl1

Ipl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ipl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available