Standard name | |
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Human Ortholog | |
Description | Component of U2 snRNP complex; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 1 | 3 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 6 | 0 | – | 4 | 8 | 12 | 15 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 5 | 6 | 8 | 23 | 11 | 29 | – | 22 | 17 | 18 | 7 | 24 | 38 | 61 | 37 | 22 | 16 | 2 | 0 | 0 | 3 | 0 | 2 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 1 |
Endosome | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 4 | 1 | 0 | 1 | 3 | 5 | 7 | 0 | 2 |
Golgi | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 2 | 0 | 1 |
Mitochondria | 2 | 5 | 1 | 0 | 0 | 51 | 9 | – | 16 | 22 | 19 | 32 | 2 | 2 | 1 | 5 | 3 | 1 | 3 | 1 | 0 | 0 | 5 | 3 |
Nucleus | 316 | 214 | 255 | 138 | 213 | 209 | 262 | – | 226 | 241 | 244 | 297 | 279 | 236 | 248 | 65 | 60 | 38 | 312 | 220 | 253 | 206 | 233 | 173 |
Nuclear Periphery | 6 | 0 | 0 | 0 | 3 | 1 | 0 | – | 1 | 3 | 3 | 7 | 3 | 1 | 3 | 3 | 3 | 1 | 0 | 0 | 0 | 2 | 2 | 3 |
Nucleolus | 24 | 9 | 3 | 2 | 4 | 15 | 14 | – | 53 | 38 | 47 | 70 | 2 | 8 | 16 | 3 | 1 | 1 | 10 | 3 | 4 | 13 | 16 | 18 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 1 | 1 | 2 | – | 0 | 0 | 0 | 0 | 2 | 1 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 |
Vac/Vac Membrane | 6 | 2 | 5 | 1 | 5 | 1 | 6 | – | 4 | 3 | 2 | 6 | 2 | 3 | 4 | 9 | 7 | 3 | 12 | 4 | 4 | 33 | 39 | 51 |
Unique Cell Count | 336 | 224 | 265 | 147 | 245 | 246 | 308 | 274 | 291 | 302 | 363 | 310 | 283 | 333 | 117 | 90 | 58 | 352 | 242 | 275 | 280 | 307 | 268 | |
Labelled Cell Count | 357 | 236 | 272 | 149 | 251 | 295 | 323 | 327 | 333 | 347 | 437 | 316 | 293 | 350 | 129 | 101 | 61 | 352 | 242 | 275 | 280 | 307 | 268 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 8.9 | 9.0 | 8.3 | 9.3 | 5.7 | 7.9 | – | 7.3 | 7.2 | 7.1 | 7.1 | 8.4 | 9.3 | 8.9 | 10.2 | 10.4 | 10.0 | 8.0 | 8.3 | 8.3 |
Std Deviation (1e-4) | 1.9 | 2.8 | 1.8 | 1.8 | 2.1 | 1.5 | 2.0 | – | 2.0 | 1.7 | 1.8 | 1.6 | 2.4 | 2.8 | 2.9 | 2.7 | 3.0 | 2.9 | 2.0 | 1.9 | 2.1 |
Intensity Change (Log2) | – | – | – | -0.12 | 0.05 | -0.66 | -0.19 | – | -0.3 | -0.32 | -0.35 | -0.33 | -0.1 | 0.05 | -0.02 | 0.19 | 0.22 | 0.16 | -0.17 | -0.11 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.3 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 3.5 | 1.4 | 3.6 | – | 3.0 | 2.1 | 2.2 | -0.3 | 2.9 | 4.8 | 6.2 | 8.4 | 6.7 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 7.6 | 0 | – | 3.6 | 4.2 | 3.8 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.1 | -3.8 | -4.4 | -4.5 | – | -5.1 | -5.1 | -5.6 | -5.5 | -2.9 | -4.9 | -7.2 | -9.9 | -7.7 | -7.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 3.0 | 2.4 | – | 6.9 | 5.4 | 6.0 | 7.0 | 0 | 1.4 | 2.5 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2738 | 2.1982 | 2.0519 | 1.5106 | 1.6436 | 1.8936 | 3.8203 | 3.84 | 3.5091 | 3.4066 | 3.0813 | 3.564 | 6.066 | 6.2719 | 5.8574 | 6.1681 | 5.2134 | 5.9125 |
Actin | 0 | 0 | 0 | 0 | 0 | 0 | 0.0112 | 0.0027 | 0.0126 | 0.0104 | 0.0033 | 0.0012 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0017 | 0.0001 | 0.0007 | 0.0002 | 0.0005 | 0.0018 | 0 | 0 | 0 | 0 | 0.0001 | 0.0004 |
Bud Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0008 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0.0009 | 0.0006 | 0.0037 | 0.0002 | 0.0003 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.003 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0028 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasmic Foci | 0 | 0 | 0 | 0 | 0 | 0 | 0.0116 | 0.001 | 0.003 | 0.0015 | 0.0166 | 0.0003 | 0 | 0 | 0 | 0 | 0.0014 | 0 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0.0031 | 0.0001 | 0.0005 | 0.0002 | 0.0004 | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0.0172 | 0.0008 | 0.0028 | 0.0052 | 0.0043 | 0.0038 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0.0069 | 0.0002 | 0.0032 | 0.0069 | 0.005 | 0.0006 | 0 | 0 | 0 | 0 | 0.0001 | 0 |
Lipid Particles | 0 | 0 | 0 | 0 | 0 | 0 | 0.0232 | 0.0009 | 0.0023 | 0.0286 | 0.0309 | 0.0004 | 0 | 0 | 0 | 0 | 0.0145 | 0 |
Mitochondria | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0044 | 0.002 | 0.0033 | 0.0108 | 0.0087 | 0.0014 | 0.0001 | 0.0001 | 0 | 0 | 0.0002 | 0 |
None | 0 | 0 | 0.0005 | 0 | 0 | 0 | 0.001 | 0.0026 | 0.0002 | 0.0001 | 0.0003 | 0.004 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0012 | 0.0023 | 0.0019 | 0.0034 | 0.0014 | 0.0004 | 0.0154 | 0.0021 | 0.0075 | 0.0022 | 0.0246 | 0.0068 | 0.0007 | 0.0015 | 0.0007 | 0.0005 | 0.0008 | 0.0006 |
Nucleolus | 0.0119 | 0.0115 | 0.007 | 0.0038 | 0.0408 | 0.0372 | 0.011 | 0.0056 | 0.0056 | 0.0052 | 0.0413 | 0.0319 | 0.0114 | 0.0101 | 0.0131 | 0.0055 | 0.029 | 0.0256 |
Nucleus | 0.9862 | 0.9851 | 0.9846 | 0.9918 | 0.9556 | 0.9588 | 0.866 | 0.9742 | 0.9421 | 0.9237 | 0.8187 | 0.937 | 0.9846 | 0.9852 | 0.9842 | 0.9922 | 0.9504 | 0.972 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.014 | 0.002 | 0.0062 | 0.0027 | 0.0329 | 0.0001 | 0 | 0 | 0 | 0 | 0.0004 | 0 |
Punctate Nuclear | 0.0004 | 0.0009 | 0.0058 | 0.0003 | 0.0015 | 0.0029 | 0.0059 | 0.0042 | 0.0043 | 0.0007 | 0.0098 | 0.0056 | 0.0028 | 0.0029 | 0.0018 | 0.0017 | 0.0028 | 0.0012 |
Vacuole | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0013 | 0.0003 | 0.0003 | 0.0004 | 0.0003 | 0.0006 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole Periphery | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0005 | 0.0006 | 0.0008 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.7724 | 5.5028 | 12.2499 | 14.1715 | 14.9646 | 3.0761 | 12.5187 | 18.3068 | 10.7811 | 12.4361 |
Translational Efficiency | 1.8975 | 2.8709 | 1.9398 | 1.5845 | 1.227 | 7.6995 | 2.0269 | 1.1625 | 2.2559 | 1.8854 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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