Standard name
Human Ortholog
Description Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.06 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0.06 0 0 0 0 0 0
Golgi 0.1 0.06 0 0 0 0 0 0 0 0 0 0 0.28 0.26 0.37 0.45 0.06 0.38 0 0.07 0 0.08 0.08 0.12
Mitochondria 0.89 0.89 0.95 0.95 0.93 0.92 0.88 0.88 0.94 0.9 0.93 0.93 0.8 0.85 0.73 0.05 0.25 0.06 0.88 0.8 0.87 0.71 0.71 0.62
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0 0.12 0 0.08 0.1 0.15 0.1 0.12 0.12 0.14 0.09 0 0 0.05 0 0.12 0 0 0 0 0 0 0
Nucleolus 0.11 0 0 0 0.11 0.11 0.13 0.13 0.11 0.11 0.07 0.09 0.05 0 0 0.1 0.19 0.19 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.31 0.12 0 0 0 0.06 0.05 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.14 0 0 0.05 0.05 0 0 0 0 0 0 0.13 0.12 0.17 0.12 0.12 0.12 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 1 0 0 1 2 1 0 0 0 0 2 6 3 9 0 0 0 0 2 1 6 10 18
Bud 2 0 3 6 10 8 13 10 15 21 22 14 4 5 2 2 0 0 0 2 0 1 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 1 0 0 0 5 3 1 4 3 4 1 0 0 0 1 0 0 – – – – – –
Cell Periphery 3 10 2 7 1 0 3 1 3 2 3 1 5 6 14 4 0 1 0 1 0 0 0 1
Cytoplasm 1 5 5 8 13 10 35 18 17 17 17 9 4 4 6 2 0 1 0 2 0 1 4 1
Endoplasmic Reticulum 2 0 2 1 2 4 1 3 1 5 4 1 5 4 4 1 0 0 0 0 0 2 0 1
Endosome 3 0 0 0 4 0 1 4 3 8 0 2 2 3 9 0 3 1 3 1 2 2 9 12
Golgi 20 11 0 4 2 0 1 0 0 0 0 1 41 60 148 19 1 6 7 13 5 14 31 42
Mitochondria 173 167 171 257 372 430 506 640 505 641 540 688 116 198 289 2 4 1 176 154 152 132 283 217
Nucleus 0 0 0 0 2 7 3 2 0 3 1 2 0 1 0 1 0 0 0 1 0 0 0 0
Nuclear Periphery 14 4 21 11 34 48 84 75 62 88 79 68 3 6 21 1 2 0 1 0 0 1 5 3
Nucleolus 21 8 8 6 46 50 76 97 57 76 43 64 7 3 8 4 3 3 1 1 0 0 2 1
Peroxisomes 0 2 2 5 0 4 3 3 2 10 1 11 4 6 14 3 5 2 4 1 7 10 21 18
SpindlePole 1 0 0 1 2 1 2 4 3 8 4 1 1 1 0 1 0 0 0 0 1 0 1 0
Vac/Vac Membrane 26 26 7 6 22 22 15 28 17 17 11 10 19 28 67 5 2 2 3 7 1 6 21 21
Unique Cell Count 194 188 180 271 402 468 577 726 538 709 581 736 145 234 398 42 16 16 201 192 176 186 399 351
Labelled Cell Count 270 235 221 312 511 591 747 886 689 899 729 875 217 328 591 46 20 17 201 192 176 186 399 351


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.7 14.0 7.9 8.7 8.8 8.4 8.0 8.1 7.4 8.3 7.8 8.1 12.1 11.3 11.7 12.3 11.4 12.3 13.7 14.0 14.1
Std Deviation (1e-4) 3.0 3.3 1.6 1.8 1.7 2.0 1.8 1.9 2.0 2.1 2.0 2.1 2.9 1.9 1.8 3.0 2.8 2.7 3.4 3.2 3.3
Intensity Change (Log2) – – – 0.15 0.16 0.1 0.02 0.04 -0.09 0.08 -0.01 0.05 0.62 0.52 0.57 0.64 0.54 0.64 0.79 0.83 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1.4 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.1 0.3 0 1.7 0 0.3 0 0.1 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 7.6 7.3 9.5 0 0 0
Mitochondria -0.1 -1.1 -1.4 -2.8 -2.7 -0.6 -2.0 -1.0 -0.8 -4.2 -3.4 -6.2 -12.7 -8.7 -10.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -3.1 -1.2 -0.5 1.0 -0.5 -0.1 0.3 0.7 -1.0 -3.3 -3.7 -2.7 0 0 0
Nucleolus -1.3 2.7 2.5 3.3 3.3 2.5 2.6 1.4 1.9 0.2 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.0 0.8 0.4 -0.9 0 -0.5 0 0 0 3.0 2.9 4.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.6551 11.5743 11.0022 8.6501 9.4919 10.33 9.7863 12.2907 11.8076 9.2966 11.4472 11.377 11.1503 11.5632 10.934 9.4796 6.961 10.7341
Actin 0.0206 0.0022 0.0094 0.0111 0.0055 0.0064 0.0775 0.0116 0.0306 0.0072 0 0.0014 0.0147 0.0019 0.0042 0.0034 0.0273 0.0003
Bud 0.0005 0.0002 0.0002 0.0003 0.0001 0.0001 0.0002 0.0002 0.0001 0.0001 0 0 0.0002 0 0.0002 0.0004 0.0013 0
Bud Neck 0.001 0.0001 0.0015 0.0003 0.0001 0.0002 0.0005 0 0.0001 0.0001 0 0 0.0016 0 0.0001 0.0123 0.0012 0.0001
Bud Periphery 0.0007 0.0004 0.0006 0.0006 0.0002 0.0002 0.0003 0.0003 0.0002 0.0002 0 0.0001 0.0004 0.0001 0.0004 0.0009 0.0029 0
Bud Site 0.0045 0.0012 0.0043 0.0006 0.0002 0.0002 0.0012 0.0005 0.0006 0.0004 0 0 0.0019 0.0001 0.0003 0.0029 0.0096 0
Cell Periphery 0.0003 0.0005 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0007 0.0002 0.0002 0.0001 0.0007 0
Cytoplasm 0.0009 0.0001 0.0002 0.0013 0.0001 0.0066 0.048 0 0 0.0001 0 0 0.0017 0 0 0.0004 0.0019 0.0001
Cytoplasmic Foci 0.0194 0.0024 0.0044 0.0134 0.0262 0.0103 0.0033 0.0003 0.0008 0.0108 0 0.0002 0.0151 0.0005 0.001 0.0089 0.0282 0
Eisosomes 0.0003 0.0002 0.0002 0.0002 0.0002 0.0002 0.0005 0.0004 0.0003 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0.0001 0.001 0.0001
Endoplasmic Reticulum 0.0008 0.0001 0.0001 0.0026 0.0001 0.0002 0.0024 0.0001 0 0.0001 0 0 0.0017 0 0.0001 0.0004 0.0022 0.0006
Endosome 0.0207 0.0124 0.0065 0.0372 0.0033 0.0058 0.0062 0.0047 0.0012 0.0085 0 0.0008 0.006 0.0014 0.0015 0.0131 0.0318 0.0022
Golgi 0.015 0.014 0.008 0.0252 0.0022 0.011 0.0106 0.0036 0.0039 0.0256 0.0001 0.0036 0.0044 0.0028 0.0027 0.0044 0.0118 0.0017
Lipid Particles 0.0124 0.0043 0.0104 0.0414 0.0462 0.0123 0.026 0.0026 0.0103 0.0125 0.0001 0.0011 0.0205 0.0107 0.004 0.0065 0.0574 0.001
Mitochondria 0.8629 0.9406 0.9338 0.8485 0.8791 0.9379 0.7823 0.9614 0.9354 0.9128 0.9992 0.989 0.907 0.959 0.9539 0.9232 0.7669 0.9772
None 0.0016 0 0.0003 0.0009 0.0003 0.0014 0.0027 0 0 0.0001 0 0 0.0017 0 0.0001 0.0003 0.007 0.0001
Nuclear Periphery 0.0006 0.0001 0.0003 0.001 0.0003 0.0002 0.0065 0.0001 0.0003 0 0 0 0.0015 0.0001 0.0002 0.0046 0.0105 0.0105
Nucleolus 0.0012 0.0003 0.0002 0.0003 0.0006 0.0002 0.0004 0.0001 0.0002 0 0 0 0.0005 0.0003 0.0001 0.0011 0.0021 0.0001
Nucleus 0.0005 0.0001 0.0002 0.0002 0.0002 0.0003 0.0046 0.0001 0.0001 0 0 0 0.0007 0.0001 0.0001 0.0016 0.0023 0.0016
Peroxisomes 0.0122 0.0035 0.0023 0.0058 0.014 0.0022 0.0222 0.0036 0.0088 0.0201 0.0002 0.0025 0.0075 0.0118 0.0066 0.0092 0.0109 0.0003
Punctate Nuclear 0.0013 0 0.0005 0.0007 0.0057 0.0004 0.0004 0 0 0.0002 0 0 0.0041 0 0.0002 0.0035 0.0153 0.0001
Vacuole 0.0082 0.0066 0.0033 0.0036 0.0048 0.0008 0.0011 0.0029 0.0009 0.0003 0 0.0002 0.0023 0.0018 0.0034 0.0015 0.0028 0.0006
Vacuole Periphery 0.0144 0.0107 0.013 0.0047 0.0104 0.0031 0.0031 0.0073 0.0061 0.0008 0.0002 0.001 0.0052 0.0092 0.0206 0.0012 0.0047 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.609 13.6997 15.2486 29.9459 21.2889 33.9153 29.6823 31.7197 25.6205 33.5796
Translational Efficiency 1.5864 1.948 1.6759 1.1928 1.6764 1.0492 1.2114 1.1354 1.7709 1.4609

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Cbp3

Cbp3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbp3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available