Standard name
Human Ortholog
Description Cyclin; interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation; PCL8 has a paralog, PCL10, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.91 0.89 0.59 0.66 0.61 0.56 0.52 0.52 0.46 0.34 0.41 0.98 0.98 0.97 0.81 0.86 0.86 0.8 0.76 0.77 0.89 0.91 0.83
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.16 0.41 0.24 0.41 0.62 0.59 0.57 0.59 0.72 0.67 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0.05 0.08 0.05 0.09 0.08 0.09 0.05 0.1 0 0 0 0 0 0.05 0.09 0.08 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0.09 0 0.07 0.22 0.05 0.06 0 0.06 0.09 0.1 0 0 0 0 0.22 0.21 0.13 0.05 0.07 0.1 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 3 8
Bud 0 1 4 1 1 0 0 8 1 3 2 4 0 1 0 0 0 0 1 1 0 0 1 15
Bud Neck 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 1 4 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 2 0 2 0 1 3 0 1 0 0 0 0 0 0
Cytoplasm 147 123 81 26 61 83 94 135 68 63 37 61 350 472 457 219 167 161 153 120 78 209 331 402
Endoplasmic Reticulum 1 2 1 1 0 1 0 1 0 0 0 0 1 5 1 11 6 5 0 0 1 0 2 4
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 6 3 2 0 3 0 1 3 6
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 2 0 1 1 0 0 0 0
Mitochondria 20 3 15 18 22 56 104 155 75 81 79 99 3 2 2 6 0 2 2 2 2 4 2 5
Nucleus 2 1 1 1 5 11 8 23 11 12 5 14 1 1 1 5 3 10 16 11 3 4 1 2
Nuclear Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 1 0 0 3 4
Vac/Vac Membrane 14 12 4 3 20 7 10 11 8 12 11 5 5 10 11 60 41 25 8 11 9 9 8 22
Unique Cell Count 168 135 91 44 92 136 168 261 131 137 110 147 356 484 469 270 194 188 192 159 101 235 364 486
Labelled Cell Count 184 142 106 50 109 158 219 336 164 176 137 184 362 492 476 312 222 207 192 159 101 235 364 486


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 5.3 3.9 3.9 4.1 3.5 3.1 3.4 3.6 3.3 3.3 3.1 5.4 5.4 5.6 7.2 8.1 7.9 4.5 4.8 5.7
Std Deviation (1e-4) 0.5 0.8 1.3 1.8 1.4 1.2 0.9 1.2 1.4 0.9 1.5 0.7 1.1 1.0 1.2 1.6 1.6 1.5 1.0 1.1 1.3
Intensity Change (Log2) – – – -0.01 0.04 -0.16 -0.35 -0.23 -0.13 -0.27 -0.27 -0.36 0.45 0.46 0.52 0.86 1.04 1.0 0.21 0.29 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -4.0 -3.7 -4.6 -5.4 -6.3 -5.8 -6.6 -7.9 -7.3 4.3 3.9 3.8 -1.7 -0.7 -0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -5.0 0 -5.0
Nucleus 0 0 0 0 2.5 0 2.4 0 2.6 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.5 0 0 0 0 1.3 1.5 0 0 0 0 3.9 3.6 2.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2961 2.1037 1.8428 1.7281 1.9018 1.9794 2.2268 1.7289 2.2813 1.4237 1.9407 2.0352 3.3096 2.6556 2.9121 2.7707 2.5665 3.0838
Actin 0.0735 0.0003 0.0067 0.0144 0.0183 0.0152 0.0749 0.0045 0.0004 0.0002 0.0048 0.003 0.0516 0.0137 0.0373 0.0004 0.0227 0.0004
Bud 0.0009 0.0002 0.0031 0.0008 0.0006 0.0003 0.0018 0.0013 0.0001 0 0.0004 0.0003 0.0016 0.0014 0.0004 0.0001 0.0124 0.0001
Bud Neck 0.0044 0.0004 0.0008 0.0139 0.0019 0.0049 0.0049 0.0003 0.0001 0.0001 0.0002 0.0165 0.0012 0.0005 0.0005 0.0003 0.0009 0.0019
Bud Periphery 0.001 0.0001 0.008 0.0015 0.0011 0.0004 0.003 0.0014 0 0 0.0005 0.0003 0.0035 0.0011 0.0003 0 0.0367 0.0001
Bud Site 0.0082 0.0051 0.0052 0.0215 0.0003 0.0004 0.0227 0.0048 0.0004 0.0001 0.0002 0.0014 0.0051 0.0098 0.0027 0.0001 0.0178 0.0001
Cell Periphery 0.0006 0.0002 0.0006 0.0012 0.0002 0.0001 0.001 0.0002 0 0 0.0001 0.0001 0.0004 0.0001 0.0001 0 0.001 0
Cytoplasm 0.3841 0.6093 0.4889 0.5983 0.4887 0.5679 0.2454 0.4305 0.1957 0.4731 0.3134 0.501 0.3264 0.537 0.4897 0.3918 0.4505 0.4568
Cytoplasmic Foci 0.0296 0.0086 0.0095 0.0068 0.0068 0.0076 0.0327 0.0114 0.0137 0.0025 0.0055 0.0154 0.0225 0.0052 0.0122 0.0049 0.0185 0.0066
Eisosomes 0.0005 0 0.0002 0.0009 0.0001 0.0001 0.0007 0.0001 0.0001 0 0.0001 0 0.0005 0.0001 0.0003 0.0001 0.0002 0
Endoplasmic Reticulum 0.0073 0.0036 0.0054 0.0046 0.0079 0.0061 0.0106 0.0065 0.0018 0.005 0.0015 0.0042 0.0153 0.0036 0.004 0.0029 0.0054 0.003
Endosome 0.0175 0.0015 0.0106 0.0053 0.0154 0.0115 0.0587 0.0089 0.0133 0.0016 0.0035 0.0144 0.0258 0.0023 0.0078 0.0024 0.0371 0.003
Golgi 0.0144 0.0001 0.0026 0.0005 0.011 0.0044 0.0231 0.0024 0.0022 0.0001 0.0031 0.0023 0.0068 0.0021 0.0023 0.0001 0.0058 0.0001
Lipid Particles 0.033 0.0003 0.0027 0.0003 0.0013 0.0032 0.0239 0.0006 0.0055 0.0001 0.0019 0.0023 0.0233 0.0001 0.0035 0.0003 0.0036 0.0001
Mitochondria 0.0165 0.0002 0.0114 0.0011 0.0045 0.007 0.0436 0.0014 0.0003 0.0002 0.0795 0.0007 0.0221 0.0025 0.0013 0.0004 0.0045 0.0003
None 0.2582 0.2949 0.3824 0.2542 0.1929 0.1878 0.3483 0.4865 0.7216 0.513 0.5737 0.3753 0.4017 0.3461 0.3306 0.5393 0.2847 0.3733
Nuclear Periphery 0.0122 0.0025 0.0072 0.0071 0.0145 0.0097 0.0153 0.0083 0.0032 0.0011 0.0011 0.0044 0.0268 0.0041 0.0067 0.0019 0.0237 0.0052
Nucleolus 0.0015 0.0003 0.0006 0.0005 0.0005 0.0012 0.0013 0.0003 0.0004 0 0.0001 0.0005 0.0009 0.0005 0.0006 0.0002 0.0024 0.0004
Nucleus 0.092 0.0672 0.0402 0.0528 0.2247 0.1552 0.0276 0.0242 0.0182 0.0018 0.0036 0.0471 0.0334 0.0634 0.0855 0.0515 0.0402 0.1407
Peroxisomes 0.0229 0.0001 0.0024 0.0006 0.0002 0.0017 0.0196 0.0011 0.0035 0 0.0049 0.001 0.0131 0.0004 0.0041 0.0001 0.0043 0.0001
Punctate Nuclear 0.015 0.0032 0.0079 0.0113 0.006 0.0131 0.0142 0.0026 0.0182 0.0004 0.0012 0.0076 0.0124 0.0047 0.0085 0.0022 0.0234 0.0065
Vacuole 0.0047 0.0016 0.0029 0.002 0.0021 0.0015 0.0235 0.0021 0.0011 0.0006 0.0006 0.0018 0.0038 0.001 0.0013 0.0008 0.0026 0.001
Vacuole Periphery 0.002 0.0001 0.0009 0.0004 0.0011 0.0007 0.003 0.0006 0.0002 0.0001 0.0002 0.0003 0.0016 0.0002 0.0003 0.0001 0.0013 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.8493 2.0008 4.9488 7.2388 8.2219 7.4563 7.9233 8.1922 11.047 8.7657
Translational Efficiency 1.5988 2.7929 1.0476 0.9359 0.8326 1.6279 0.8639 0.9518 0.9712 1.1595

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cyclin; interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation; PCL8 has a paralog, PCL10, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (28%), nucleus (7%), mixed (52%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Pcl8

Pcl8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pcl8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available