Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0.06 – 0.06 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.96 0.97 0.94 0.91 0.75 – 0.73 0.62 0.78 0.48 0.96 0.98 0.96 0.98 0.93 0.99 0.95 0.94 0.94 0.9 0.89 0.91
Endoplasmic Reticulum 0 0 0.05 0.05 0 0 0.09 – 0.09 0.13 0.05 0.13 0 0 0 0.05 0 0 0 0 0 0 0.05 0.06
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.06 – 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.14 0.13 0.11 0.29 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.09 – 0 0.1 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 7 – 11 9 6 10 0 0 0 0 0 0 0 0 0 0 2 0
Bud 0 1 1 0 0 1 1 – 1 1 0 5 1 0 1 0 0 0 0 0 0 1 0 2
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 3 6 13 9 15 – 13 13 11 14 5 8 12 5 5 3 0 0 1 1 0 0
Cytoplasm 206 308 170 143 225 187 201 – 164 143 140 108 288 351 338 169 128 112 196 300 168 225 313 349
Endoplasmic Reticulum 5 6 9 7 10 6 25 – 20 29 9 28 12 9 6 8 2 2 5 2 4 9 17 23
Endosome 0 0 0 0 0 0 0 – 0 1 0 1 0 0 2 0 1 0 0 0 0 0 0 0
Golgi 0 1 1 0 0 1 15 – 10 16 3 6 1 1 0 2 1 0 0 1 0 1 1 0
Mitochondria 0 0 0 0 0 7 2 – 32 29 20 65 1 1 0 2 0 1 0 0 0 0 1 1
Nucleus 0 0 1 0 0 2 2 – 2 1 2 1 0 0 0 0 0 0 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 0 1 0 0 0 0 0 – 0 0 0 3 0 0 0 0 0 0 0 0 0 1 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Vac/Vac Membrane 1 0 0 1 7 4 25 – 9 24 5 24 0 1 2 1 4 0 2 6 0 3 4 3
Unique Cell Count 209 316 178 147 239 206 268 225 231 179 223 299 359 351 172 137 113 207 318 179 250 352 386
Labelled Cell Count 212 318 185 157 255 218 293 262 266 196 265 308 371 361 187 141 118 207 318 179 250 352 386


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 64.6 50.3 41.1 44.4 38.5 19.8 21.7 – 14.9 13.8 13.2 11.6 51.2 44.4 42.4 37.1 37.7 41.8 44.7 45.1 46.3
Std Deviation (1e-4) 14.0 11.5 9.2 10.4 8.9 6.5 4.8 – 3.3 2.6 3.0 2.4 11.4 10.2 11.9 9.6 11.1 12.9 12.4 13.0 10.7
Intensity Change (Log2) – – – 0.11 -0.1 -1.05 -0.92 – -1.47 -1.58 -1.64 -1.82 0.32 0.11 0.04 -0.15 -0.13 0.02 0.12 0.13 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.0 1.5 2.1 – 2.1 2.0 2.2 2.3 0 0 1.1 0 0 0
Cytoplasm 0.8 -0.6 -1.8 -5.7 – -6.0 -7.9 -4.8 -10.2 0.4 1.5 0.4 1.5 -0.8 1.7
Endoplasmic Reticulum -0.1 -0.4 -1.1 1.7 – 1.5 2.6 0 2.6 -0.5 -1.5 -2.2 -0.2 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 2.8 – 0 3.2 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 5.2 4.9 4.6 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 4.2 – 0 4.4 0 4.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 50.4176 70.1099 63.5592 60.5528 61.2589 64.4673 51.3362 79.9762 72.3548 62.6098 79.6996 71.3678 65.6858 80.1815 77.5638 72.7606 70.2925 78.6534
Actin 0.0042 0.0005 0.0004 0.0002 0.0017 0.0002 0.038 0.0008 0.0014 0.0172 0 0.0042 0.001 0.0002 0.0012 0.0003 0.0002 0.0009
Bud 0.002 0.0002 0.0006 0.0021 0.0032 0.0002 0.0015 0.0003 0.0017 0.001 0.0001 0.002 0.0007 0.0002 0.0009 0.0013 0.0003 0.0007
Bud Neck 0.0012 0.0003 0.0005 0.0008 0.0007 0.0008 0.0006 0.0004 0.0008 0.0007 0.0001 0.0021 0.0003 0.0003 0.0004 0.001 0.0004 0.0011
Bud Periphery 0.0035 0.0001 0.0006 0.0008 0.0012 0.0001 0.0021 0.0001 0.0011 0.0005 0 0.0031 0.0008 0.0001 0.0005 0.0006 0.0001 0.0004
Bud Site 0.0046 0.0002 0.0004 0.0005 0.0017 0 0.003 0.0002 0.0005 0.0003 0 0.0002 0.0003 0.0001 0.0002 0.0003 0.0001 0.0001
Cell Periphery 0.0124 0.0033 0.005 0.0042 0.0003 0.0003 0.0019 0.002 0.0039 0.0128 0 0.0014 0.0042 0.0024 0.001 0.0042 0.0004 0.0007
Cytoplasm 0.7495 0.9624 0.8741 0.7419 0.8956 0.9167 0.654 0.9641 0.9134 0.6982 0.9769 0.8994 0.8419 0.9598 0.9238 0.8638 0.9278 0.8754
Cytoplasmic Foci 0.0234 0.0012 0.0064 0.0078 0.0139 0.008 0.0174 0.0009 0.0038 0.0055 0.0041 0.0052 0.0024 0.0012 0.0023 0.0033 0.0024 0.005
Eisosomes 0.0004 0 0.0001 0 0 0 0.0003 0.0006 0 0 0 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0402 0.016 0.0221 0.0036 0.0061 0.0209 0.0411 0.0076 0.0072 0.0109 0.0003 0.0367 0.0559 0.016 0.0149 0.006 0.0109 0.0378
Endosome 0.0151 0.0015 0.008 0.0111 0.0167 0.0163 0.0305 0.0013 0.0141 0.0377 0.0048 0.011 0.0115 0.0023 0.0076 0.0139 0.0074 0.0254
Golgi 0.003 0.0004 0.001 0.0003 0.0014 0.0008 0.0093 0.0007 0.0086 0.0234 0.0001 0.0023 0.0018 0.0004 0.0012 0.0009 0.0002 0.0042
Lipid Particles 0.0086 0.0016 0.0027 0.0008 0.0003 0.0002 0.0251 0.0002 0.0011 0.0006 0 0.0006 0.0008 0.0002 0.0002 0.0004 0.0001 0.0001
Mitochondria 0.0077 0.0001 0.0097 0.0007 0.0024 0.0007 0.0113 0.0005 0.0056 0.006 0 0.0039 0.0064 0.0002 0.0011 0.0019 0.0003 0.0056
None 0.0057 0.0001 0.0031 0.0035 0.0007 0.0006 0.0069 0.002 0.0009 0.0205 0.0001 0.0006 0.0011 0.0001 0.0002 0.0004 0.0003 0.0003
Nuclear Periphery 0.01 0.0011 0.004 0.0061 0.0043 0.0027 0.0531 0.0037 0.0048 0.0079 0.0008 0.011 0.0109 0.0011 0.0026 0.0039 0.0055 0.0037
Nucleolus 0.0021 0 0.0037 0.0006 0.0001 0.0019 0.0012 0.0001 0.0001 0.0002 0 0.0001 0.0007 0 0 0.0003 0.0001 0
Nucleus 0.0043 0.0007 0.003 0.0035 0.0014 0.0037 0.0179 0.0042 0.0023 0.0039 0.0007 0.0019 0.0029 0.0007 0.0013 0.0021 0.0025 0.0014
Peroxisomes 0.0009 0 0.0004 0.0001 0.0008 0 0.0077 0 0.0002 0.0003 0 0.0001 0.0001 0 0 0.0001 0 0
Punctate Nuclear 0.0014 0 0.0003 0.0002 0.0001 0.0003 0.0231 0.0001 0.0001 0.0003 0 0.0001 0.0002 0 0 0 0 0
Vacuole 0.0797 0.0097 0.0451 0.185 0.0356 0.0201 0.0407 0.0092 0.0229 0.1229 0.0095 0.009 0.0448 0.0129 0.0315 0.0783 0.0342 0.0262
Vacuole Periphery 0.0201 0.0007 0.0087 0.0263 0.012 0.0056 0.0133 0.0012 0.0054 0.029 0.0024 0.0052 0.0114 0.0017 0.009 0.0172 0.0068 0.0109

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3538.8239 3729.8292 4610.9584 3411.6122 3828.0905 3954.2064 3287.4328 2893.2086 3401.1097 2806.2015
Translational Efficiency 0.6993 0.7292 0.6697 0.8939 0.826 0.6842 0.7832 0.9774 0.7727 0.9037

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl1a

Rpl1a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl1a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available