Standard name
Human Ortholog
Description UDP-glucose:dolichyl-phosphate glucosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum; human ortholog ALG5 can partially complement yeast alg5 mutant
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.14 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.07 0.06 0 0 0 – 0 0 0 0.05 0.12 0.17 0.5 0.34 0.14 0 0 0 0 0 0
Cytoplasm 0.1 0.14 0.07 0.2 0.17 0.21 0.15 0.2 – 0.25 0.28 0.28 0.16 0.1 0.15 0.25 0.28 0.45 0 0 0 0 0.06 0.07
Endoplasmic Reticulum 0.74 0.88 0.87 0.5 0.65 0.44 0.41 0.28 – 0.3 0.18 0.24 0.8 0.87 0.75 0 0 0 0.74 0.9 0.92 0.88 0.81 0.76
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.06 0.07 0.11 0 0 0 0 0 0
Mitochondria 0 0 0.13 0.24 0.2 0.34 0.44 0.34 – 0.31 0.46 0.49 0 0 0 0.19 0.11 0.07 0.05 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.07 0 0 0 0.06 0 – 0 0 0 0 0 0 0.12 0 0 0.11 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.05 0 0.14 0.13 0.13 0.26 0.27 – 0.27 0.21 0.13 0.08 0 0 0 0.07 0.3 0 0 0 0 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 1 7 9 15 16 4 9 – 2 5 12 3 7 11 0 12 1 0 2 3 1 3 3
Bud 0 2 1 2 9 20 6 12 – 19 20 16 0 0 0 0 2 0 0 0 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 2 7 19 34 21 17 14 – 28 16 18 8 37 64 8 30 6 0 0 0 0 0 2
Cytoplasm 10 23 13 56 94 121 108 139 – 171 137 190 25 32 54 4 24 20 1 4 1 2 14 20
Endoplasmic Reticulum 72 144 172 140 368 254 287 187 – 205 87 159 122 275 275 0 2 1 99 156 196 129 215 206
Endosome 0 1 1 4 4 3 2 9 – 7 0 1 1 0 0 0 0 0 2 1 0 0 0 0
Golgi 0 0 0 1 0 0 0 0 – 0 0 0 0 0 1 1 6 5 1 0 0 0 1 1
Mitochondria 2 2 25 68 111 192 311 229 – 211 226 327 1 0 3 3 10 3 6 2 3 5 10 6
Nucleus 1 0 0 0 0 9 5 11 – 7 2 6 4 1 2 0 1 0 0 0 0 0 0 1
Nuclear Periphery 2 4 14 8 14 13 42 27 – 28 13 12 0 3 6 2 3 0 14 0 0 1 2 1
Nucleolus 0 0 1 1 0 1 1 5 – 0 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 11 8 5 38 73 73 181 182 – 182 101 90 12 2 13 0 6 13 5 3 1 4 12 20
Unique Cell Count 97 164 198 280 566 573 708 680 686 491 667 153 317 366 16 87 44 134 174 213 148 266 272
Labelled Cell Count 101 187 246 346 722 723 964 824 860 608 835 176 357 429 18 96 49 134 174 213 148 266 272


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 11.6 7.3 7.3 7.1 6.4 5.9 6.3 – 6.0 5.9 5.4 9.5 9.5 9.5 13.6 14.3 14.5 10.7 10.4 10.2
Std Deviation (1e-4) 1.9 1.7 1.0 1.2 1.3 1.2 1.2 1.2 – 1.2 1.2 1.1 1.2 1.2 1.2 2.3 2.4 2.8 1.4 1.3 1.5
Intensity Change (Log2) – – – 0.0 -0.03 -0.18 -0.3 -0.21 – -0.28 -0.31 -0.42 0.39 0.39 0.39 0.91 0.98 0.99 0.56 0.51 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 -0.6 -0.5 0 0 – 0 0 0 0 -0.9 -0.3 0 3.2 0
Bud 0 0 2.2 0 0 – 0 2.5 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 1.3 0.1 -0.9 0 – 0.3 -0.2 -0.6 0.8 3.2 4.8 0 7.2 0
Cytoplasm 4.1 3.5 4.7 3.2 4.5 – 5.6 6.1 6.4 2.9 1.4 2.9 0 4.9 6.8
Endoplasmic Reticulum -8.3 -5.8 -10.4 -11.5 -15.0 – -14.3 -17.0 -16.0 -1.8 0 -3.3 -8.4 -13.5 -11.2
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 2.2 5.6 8.1 5.7 – 5.1 8.2 9.2 -4.2 -6.5 -6.2 0 -0.3 -1.1
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.2 -3.0 -3.2 -0.6 -1.8 – -1.7 -2.7 -3.8 -3.4 -3.8 -3.3 0 -1.2 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 4.2 4.1 4.1 7.1 7.3 – 7.3 5.9 4.3 2.3 0 0.7 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8007 5.6543 5.4401 5.0327 5.0952 5.1977 5.7295 6.7968 6.2072 5.9658 5.6483 6.4272 4.0434 4.8179 4.5087 4.346 3.754 4.2672
Actin 0.0053 0.0007 0.004 0.0005 0.0175 0.0007 0.0148 0.0004 0.0145 0.01 0.0365 0.0003 0.0023 0 0.0024 0.0002 0.0257 0.0026
Bud 0.0001 0.0002 0.0002 0.0001 0.0008 0 0.0003 0 0.0001 0.0001 0.0001 0 0 0 0 0 0.0001 0
Bud Neck 0.0022 0.0004 0.0015 0.0006 0.0007 0.0006 0.0024 0.0001 0.0003 0.0006 0.0009 0.0005 0.0002 0 0.0008 0.007 0.0006 0.0003
Bud Periphery 0.0002 0.0001 0.0005 0 0.0006 0.0001 0.0005 0.0001 0.0001 0.0002 0.0004 0.0001 0.0001 0 0.0001 0 0.0003 0
Bud Site 0.0005 0.0116 0.0031 0 0.0004 0 0.0017 0.0004 0.0004 0.0008 0.001 0 0.0002 0 0.0003 0 0.0004 0
Cell Periphery 0.0006 0.0025 0.0009 0.0004 0.0006 0.0006 0.0011 0.0013 0.0013 0.0037 0.004 0.0005 0.0005 0.0003 0.0007 0.0005 0.0009 0.0002
Cytoplasm 0.0712 0.1 0.0374 0.0787 0.0741 0.05 0.0265 0.0687 0.0458 0.0517 0.0528 0.0406 0.0176 0.0235 0.0348 0.0409 0.014 0.0285
Cytoplasmic Foci 0.0119 0.0028 0.0065 0.0062 0.0032 0.0033 0.0062 0.0017 0.0079 0.0134 0.0107 0.001 0.005 0.001 0.002 0.0004 0.0099 0.0009
Eisosomes 0.0002 0.0001 0.0002 0 0.0002 0 0.0006 0 0.0002 0.0003 0.0008 0 0.0001 0 0 0 0.0006 0
Endoplasmic Reticulum 0.7921 0.8101 0.8605 0.8805 0.7846 0.9018 0.8613 0.8912 0.8697 0.8269 0.7646 0.9354 0.9198 0.9434 0.926 0.9326 0.8276 0.9376
Endosome 0.0332 0.0109 0.0182 0.0035 0.0167 0.0116 0.0142 0.0041 0.0164 0.0177 0.0165 0.0049 0.012 0.0023 0.0072 0.0025 0.0174 0.0055
Golgi 0.0036 0.001 0.005 0.0002 0.005 0.0009 0.004 0.0008 0.0053 0.0031 0.012 0.0004 0.0022 0.0002 0.0011 0.0001 0.0067 0.0016
Lipid Particles 0.0145 0.0046 0.01 0.0008 0.0369 0.0024 0.0069 0.008 0.0106 0.0209 0.0255 0.0034 0.0075 0.0169 0.0076 0.0008 0.0219 0.001
Mitochondria 0.0016 0.0023 0.0132 0.0002 0.0043 0.0008 0.0026 0.0064 0.001 0.0007 0.0066 0.0002 0.0007 0.0001 0.0004 0.0001 0.0028 0.0003
None 0.0116 0.0161 0.0091 0.0112 0.0044 0.0019 0.0226 0.0007 0.0033 0.0177 0.039 0.0038 0.0052 0.0002 0.0009 0.0003 0.0072 0.0004
Nuclear Periphery 0.0281 0.0167 0.0114 0.0075 0.0321 0.0145 0.016 0.0054 0.0091 0.0076 0.0049 0.005 0.0188 0.0085 0.0094 0.0101 0.0304 0.0147
Nucleolus 0.0024 0.007 0.0003 0.0002 0.0004 0.0001 0.0008 0 0.0001 0.0002 0.0003 0 0.0001 0 0.0001 0 0.0008 0
Nucleus 0.0052 0.0059 0.002 0.0026 0.0027 0.0033 0.0024 0.0007 0.003 0.0014 0.0015 0.0007 0.001 0.0005 0.0011 0.0008 0.0023 0.0017
Peroxisomes 0.0031 0.0003 0.0078 0 0.0021 0.0002 0.0074 0.0066 0.0039 0.0165 0.0168 0 0.0016 0 0.0002 0 0.0099 0
Punctate Nuclear 0.0059 0.0014 0.0007 0.0042 0.0042 0.004 0.0025 0.0001 0.0024 0.0021 0.0014 0.0001 0.001 0.0002 0.0007 0.0001 0.0144 0.0014
Vacuole 0.0054 0.0043 0.0068 0.0024 0.004 0.0028 0.0045 0.003 0.0041 0.0038 0.0034 0.0029 0.0034 0.0026 0.0039 0.003 0.0041 0.0027
Vacuole Periphery 0.0012 0.001 0.0008 0.0003 0.0045 0.0005 0.0009 0.0002 0.0006 0.0004 0.0006 0.0003 0.0009 0.0002 0.0005 0.0005 0.0018 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.5249 46.129 23.3054 37.0386 30.6051 34.0165 41.4311 36.5029 32.6893 42.4938
Translational Efficiency 1.1836 0.9021 1.1317 0.5649 1.0424 0.9358 0.7924 0.6274 0.7105 0.7248

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description UDP-glucose:dolichyl-phosphate glucosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum; human ortholog ALG5 can partially complement yeast alg5 mutant
Localization
Cell Percentages ER (99%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Alg5

Alg5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available