Standard name
Human Ortholog
Description Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0.08 0.12 0.17 0.27 0.37 0.51 0.38 0.45 0.49 0.53 0.06 0.06 0.1 0.12 0.06 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0.16 0.22 0.23 0.44 0.44 0.17 0.1 0.1 0 0.08 0 0 0.48 0.65 0.48 0.41 0.4 0.39 0.5 0.34 0.44 0.59 0.53 0.41
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.13 0.1 0 0 0 0 0 0
Mitochondria 0.45 0.08 0.1 0.19 0.06 0.39 0.52 0.31 0.68 0.47 0.65 0.33 0 0 0 0.07 0.09 0.07 0 0 0.08 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0
Nucleolus 0.1 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.08 0 0 0 0 0 0 0 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.63 0.73 0.64 0.47 0.54 0.39 0.26 0.25 0.17 0.27 0.15 0.21 0.68 0.61 0.68 0.62 0.63 0.62 0.37 0.53 0.36 0.31 0.36 0.48
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 1 0 1 0 0 0 1 3 0 0 1 6 4 1 0 0 1 0 1
Bud 3 1 0 1 0 7 11 3 13 6 8 6 0 0 1 1 3 1 0 4 0 2 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 3 3 3 2 0 4 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 19 3 22 49 71 130 163 67 120 128 159 19 29 51 39 16 35 1 3 0 2 4 6
Endoplasmic Reticulum 1 3 5 3 1 3 1 4 1 0 1 9 2 3 4 12 17 10 0 0 0 0 2 3
Endosome 28 50 9 78 128 44 36 33 5 21 1 11 147 306 233 134 116 124 97 83 41 147 212 197
Golgi 0 1 1 0 0 1 0 0 0 0 0 0 0 2 1 19 37 33 2 3 0 1 1 0
Mitochondria 78 18 4 34 18 102 186 100 120 126 170 100 7 3 3 24 25 23 6 6 7 8 17 6
Nucleus 4 4 1 0 6 3 2 2 1 1 1 6 2 5 5 1 1 0 0 4 0 0 1 3
Nuclear Periphery 5 6 3 1 0 1 0 1 0 2 0 0 2 1 6 1 0 1 8 4 7 4 6 13
Nucleolus 17 19 1 4 0 0 1 0 0 0 0 0 6 7 8 9 13 11 0 1 0 0 1 2
Peroxisomes 0 0 0 1 0 2 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0
SpindlePole 4 4 0 14 11 5 8 6 1 2 0 1 15 23 20 16 8 8 1 2 0 2 5 2
Vac/Vac Membrane 109 164 25 84 156 103 92 81 30 72 40 63 207 285 330 201 180 197 71 132 33 77 144 231
Unique Cell Count 173 225 39 178 288 263 356 319 176 267 261 302 305 471 488 323 287 317 194 248 94 250 404 481
Labelled Cell Count 256 289 53 242 369 343 470 397 241 352 349 361 410 666 662 459 422 447 194 248 94 250 404 481


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.8 6.0 6.3 6.6 5.4 5.1 5.9 4.7 5.0 4.6 5.2 6.8 7.1 7.0 8.8 9.8 9.9 6.2 6.6 7.3
Std Deviation (1e-4) 0.8 1.5 1.2 1.7 1.7 1.6 1.5 1.6 0.9 1.0 1.1 1.0 1.0 1.2 1.0 1.8 1.8 2.0 1.2 1.2 1.5
Intensity Change (Log2) – – – 0.07 0.13 -0.17 -0.25 -0.03 -0.37 -0.25 -0.38 -0.21 0.18 0.24 0.21 0.56 0.71 0.72 0.05 0.13 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.5 2.6 3.6 5.1 3.7 4.4 4.9 5.3 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 2.4 2.5 -1.0 0 0 0 0 0 0 3.0 5.2 3.0 2.2 2.1 2.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.3 0 3.5 5.0 2.7 0 4.4 0 2.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -1.2 -2.9 -5.0 -5.0 -6.1 -4.7 -6.9 -5.8 0.5 -0.4 0.5 -0.2 -0.2 -0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0924 2.9591 2.651 2.1704 2.1891 2.5191 1.2434 2.168 1.8977 1.1427 1.4748 1.76 0.9409 1.52 1.1195 1.0865 0.5689 0.9732
Actin 0.0045 0.0002 0.0014 0 0.0002 0.0039 0.008 0 0.0015 0 0.0247 0 0.0231 0 0.0079 0.0005 0 0.0001
Bud 0.0001 0.0024 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0006 0.0001 0.0012 0.0001 0.0003 0 0 0.0001
Bud Neck 0.0007 0.0004 0.0002 0.0001 0.0001 0.0003 0.0004 0.0002 0.0007 0.0001 0.0007 0.0004 0.0005 0.0002 0.0002 0.0001 0.0001 0.0002
Bud Periphery 0.0003 0.0028 0.0002 0 0.0001 0.0002 0.0002 0.0001 0.0002 0 0.0015 0.0001 0.001 0.0001 0.0003 0 0 0.0001
Bud Site 0.0008 0.0095 0.0004 0 0.0002 0.0002 0.0003 0.0003 0.0007 0.0001 0.0014 0.0001 0.0066 0.0003 0.002 0.0001 0.0001 0.0001
Cell Periphery 0.0002 0.0004 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0 0.0001 0.0001 0.0002 0.0002 0.0002 0 0 0.0001
Cytoplasm 0.0069 0.0128 0.0129 0.0019 0.002 0.0047 0.0104 0.0158 0.0123 0.0098 0.0026 0.0131 0.0314 0.015 0.0186 0.0148 0.003 0.0062
Cytoplasmic Foci 0.0567 0.0685 0.0644 0.0362 0.0468 0.0653 0.0522 0.063 0.0576 0.0363 0.0464 0.058 0.0501 0.0592 0.0481 0.0367 0.0477 0.0736
Eisosomes 0.0002 0 0 0 0 0 0.0001 0 0.0002 0 0.0003 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.002 0.0022 0.0012 0.0006 0.001 0.0013 0.0029 0.0039 0.003 0.0007 0.0032 0.0022 0.0045 0.0025 0.0026 0.0023 0.0005 0.0015
Endosome 0.7192 0.6997 0.7005 0.742 0.7869 0.6789 0.695 0.6769 0.6981 0.769 0.7088 0.7158 0.6241 0.6312 0.6755 0.7776 0.8142 0.7075
Golgi 0.0449 0.0319 0.0531 0.0462 0.0393 0.0568 0.0266 0.0225 0.0287 0.0255 0.0306 0.042 0.0278 0.0225 0.0343 0.0353 0.0155 0.0482
Lipid Particles 0.0377 0.0169 0.0283 0.0603 0.0165 0.0541 0.0531 0.0518 0.0212 0.0238 0.0329 0.0416 0.0285 0.0189 0.0164 0.0076 0.0082 0.0405
Mitochondria 0.0159 0.01 0.0426 0.0169 0.0374 0.0311 0.0078 0.0074 0.012 0.0007 0.0054 0.006 0.0069 0.0015 0.0158 0.0016 0.0002 0.0036
None 0.0029 0.003 0.0077 0.0001 0.0005 0.0037 0.0025 0.0034 0.0085 0.0002 0.0033 0.0032 0.0107 0.0023 0.0015 0.004 0.0001 0.0032
Nuclear Periphery 0.0057 0.0059 0.0014 0.0007 0.0015 0.0039 0.0065 0.0089 0.0048 0.0015 0.0191 0.0068 0.0181 0.0071 0.0045 0.0051 0.002 0.0026
Nucleolus 0.0029 0.0016 0.0002 0.0001 0.0003 0.0006 0.0007 0.0004 0.0015 0.0002 0.0011 0.0005 0.0005 0.0006 0.0004 0.0001 0.0001 0.0003
Nucleus 0.002 0.0017 0.0004 0.0001 0.0004 0.001 0.0029 0.0012 0.0022 0.0003 0.0022 0.0012 0.0065 0.0016 0.0009 0.0018 0.0001 0.0003
Peroxisomes 0.0067 0.0039 0.0074 0.0025 0.0063 0.0102 0.0089 0.0072 0.0031 0.0014 0.0128 0.0028 0.0116 0.0015 0.0066 0.0007 0.0005 0.0015
Punctate Nuclear 0.0013 0.0004 0.0002 0.0001 0.0008 0.0007 0.0016 0.0005 0.0014 0.0002 0.0455 0.001 0.0026 0.0004 0.0003 0.004 0.0001 0.0006
Vacuole 0.0636 0.0991 0.051 0.0659 0.0362 0.0496 0.0874 0.1063 0.1072 0.0941 0.0364 0.0665 0.1107 0.1772 0.1125 0.0729 0.0729 0.0723
Vacuole Periphery 0.025 0.0269 0.0264 0.026 0.0235 0.0332 0.0319 0.0301 0.0348 0.0361 0.0204 0.0387 0.033 0.0577 0.0512 0.0346 0.0348 0.0375

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.5422 4.504 4.679 5.8392 5.8792 8.0569 7.2864 7.9915 7.8616 6.9645
Translational Efficiency 0.9136 0.5926 0.6088 0.7722 0.5085 0.5371 0.6778 0.4373 0.7383 0.5584

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
Localization
Cell Percentages vacuolar membrane (37%), endosome (9%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Env7

Env7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Env7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available