ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 1 | 12 | 1 | 6 | 8 |
Bud | 1 | 0 | 2 | 1 | 7 | 12 | 10 | 4 | 20 | 16 | 16 | 17 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 11 | 2 | 9 | 18 |
Bud Neck | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 5 | 2 | 6 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 0 | 2 | 4 | 12 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 |
Cytoplasm | 454 | 204 | 504 | 202 | 290 | 414 | 397 | 491 | 436 | 389 | 282 | 258 | 394 | 348 | 499 | 77 | 83 | 79 | 385 | 183 | 306 | 262 | 386 | 431 |
Endoplasmic Reticulum | 3 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 2 | 10 | 13 | 6 | 2 | 1 | 9 | 0 | 1 | 3 |
Endosome | 0 | 0 | 15 | 7 | 8 | 3 | 5 | 26 | 9 | 3 | 2 | 2 | 4 | 4 | 9 | 11 | 9 | 11 | 7 | 1 | 14 | 1 | 4 | 7 |
Golgi | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 1 | 1 | 2 | 7 | 0 | 1 | 2 |
Mitochondria | 66 | 17 | 45 | 61 | 112 | 400 | 472 | 271 | 449 | 608 | 538 | 492 | 0 | 0 | 0 | 8 | 3 | 6 | 26 | 9 | 163 | 5 | 20 | 24 |
Nucleus | 4 | 7 | 4 | 9 | 10 | 37 | 45 | 38 | 33 | 30 | 44 | 26 | 1 | 0 | 1 | 23 | 27 | 18 | 32 | 31 | 25 | 16 | 18 | 8 |
Nuclear Periphery | 2 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 1 | 6 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 2 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 2 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 2 | 1 | 4 | 2 | 21 | 12 | 10 | 11 | 11 | 1 | 0 | 0 | 1 | 1 | 0 | 13 | 10 | 18 | 6 | 14 | 17 |
Vac/Vac Membrane | 46 | 45 | 118 | 62 | 47 | 96 | 74 | 97 | 109 | 91 | 68 | 55 | 18 | 26 | 49 | 62 | 67 | 66 | 65 | 44 | 82 | 28 | 47 | 43 |
Unique Cell Count | 523 | 249 | 613 | 289 | 409 | 767 | 795 | 806 | 857 | 905 | 746 | 676 | 405 | 358 | 523 | 157 | 175 | 152 | 563 | 299 | 686 | 337 | 528 | 590 |
Labelled Cell Count | 576 | 277 | 694 | 344 | 478 | 978 | 1009 | 951 | 1078 | 1155 | 984 | 875 | 420 | 380 | 561 | 194 | 206 | 189 | 563 | 299 | 686 | 337 | 528 | 590 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.3 | 4.5 | 4.4 | 4.4 | 3.5 | 3.4 | 3.5 | 3.3 | 3.1 | 2.9 | 2.8 | 5.8 | 5.8 | 5.5 | 6.3 | 5.9 | 6.2 | 5.7 | 5.9 | 6.1 |
Std Deviation (1e-4) | 0.5 | 0.9 | 4.8 | 2.1 | 2.1 | 1.6 | 1.5 | 1.1 | 1.0 | 0.8 | 1.0 | 0.6 | 1.2 | 1.5 | 1.2 | 1.2 | 1.2 | 1.6 | 1.6 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.05 | -0.36 | -0.4 | -0.38 | -0.44 | -0.55 | -0.65 | -0.69 | 0.36 | 0.36 | 0.28 | 0.48 | 0.37 | 0.46 | 0.34 | 0.37 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.3 | 5.2 | 6.2 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1721 | 1.0874 | 0.6932 | 0.4308 | 0.446 | 0.6499 | 0.013 | 1.0546 | 0.7315 | 0.3056 | 0.4628 | 0.8046 | -0.5384 | 0.5372 | 0.1056 | 0.0997 | -0.3676 | 0.2387 |
Actin | 0.0047 | 0.001 | 0.0049 | 0.0003 | 0.0002 | 0.0003 | 0.0008 | 0.0004 | 0.003 | 0.0003 | 0.0009 | 0.0008 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0005 | 0.0013 |
Bud | 0.0001 | 0.0043 | 0.0024 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.004 | 0.0019 | 0.0001 | 0.0001 | 0.0003 |
Bud Neck | 0.0002 | 0.0004 | 0.0004 | 0.0004 | 0.0012 | 0.0024 | 0.0052 | 0.001 | 0.0007 | 0.0013 | 0.002 | 0.01 | 0.0048 | 0.0008 | 0.0004 | 0.0004 | 0.0003 | 0.0067 |
Bud Periphery | 0.0001 | 0.0019 | 0.0006 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0006 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0009 | 0.0012 | 0 | 0.0001 | 0.0003 |
Bud Site | 0.0002 | 0.0083 | 0.004 | 0.0002 | 0.0002 | 0.0005 | 0.0011 | 0.0031 | 0.0019 | 0.0005 | 0.0002 | 0.0005 | 0.0098 | 0.0084 | 0.0015 | 0.0003 | 0.0004 | 0.0003 |
Cell Periphery | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasm | 0.2816 | 0.3442 | 0.3101 | 0.3292 | 0.3779 | 0.3338 | 0.2963 | 0.3452 | 0.2398 | 0.3683 | 0.248 | 0.3198 | 0.3441 | 0.3776 | 0.3404 | 0.4564 | 0.2063 | 0.4299 |
Cytoplasmic Foci | 0.0116 | 0.0176 | 0.0123 | 0.0094 | 0.0109 | 0.0255 | 0.0111 | 0.0081 | 0.0092 | 0.0129 | 0.0119 | 0.0123 | 0.0107 | 0.0066 | 0.0118 | 0.0113 | 0.0184 | 0.0114 |
Eisosomes | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.0052 | 0.005 | 0.0053 | 0.0065 | 0.0035 | 0.0041 | 0.0035 | 0.0035 | 0.0027 | 0.0029 | 0.0038 | 0.0059 | 0.0051 | 0.0036 | 0.0055 | 0.0041 | 0.0045 |
Endosome | 0.0057 | 0.0095 | 0.0101 | 0.0078 | 0.0108 | 0.0071 | 0.0054 | 0.0065 | 0.0042 | 0.0081 | 0.0078 | 0.0073 | 0.0036 | 0.0068 | 0.0064 | 0.0082 | 0.0078 | 0.0071 |
Golgi | 0.0006 | 0.0004 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0003 | 0.0004 |
Lipid Particles | 0.0036 | 0.0045 | 0.0012 | 0.0003 | 0.0003 | 0.0007 | 0.0014 | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0002 | 0.0003 | 0.0013 | 0.0002 | 0.0003 | 0.0003 |
Mitochondria | 0.0004 | 0.0011 | 0.0017 | 0.001 | 0.0007 | 0.0008 | 0.0008 | 0.0008 | 0.0013 | 0.001 | 0.0018 | 0.0008 | 0.0005 | 0.0007 | 0.0005 | 0.0011 | 0.0004 | 0.0016 |
None | 0.5649 | 0.4344 | 0.5223 | 0.5556 | 0.318 | 0.4546 | 0.4611 | 0.3932 | 0.4594 | 0.2747 | 0.3434 | 0.3366 | 0.4849 | 0.4074 | 0.4084 | 0.2524 | 0.5484 | 0.2613 |
Nuclear Periphery | 0.0049 | 0.0145 | 0.0098 | 0.0069 | 0.0144 | 0.0055 | 0.0096 | 0.008 | 0.0121 | 0.0108 | 0.0104 | 0.0096 | 0.0128 | 0.0093 | 0.0065 | 0.0392 | 0.0037 | 0.0087 |
Nucleolus | 0.0006 | 0.0024 | 0.0017 | 0.001 | 0.0008 | 0.0015 | 0.0018 | 0.0038 | 0.0025 | 0.0046 | 0.0085 | 0.0039 | 0.001 | 0.0014 | 0.0009 | 0.0009 | 0.0005 | 0.0023 |
Nucleus | 0.0992 | 0.129 | 0.0943 | 0.0732 | 0.2448 | 0.1413 | 0.166 | 0.1775 | 0.2077 | 0.2636 | 0.2673 | 0.2342 | 0.1037 | 0.1479 | 0.202 | 0.166 | 0.2005 | 0.2297 |
Peroxisomes | 0.0096 | 0.0006 | 0.0011 | 0.0001 | 0.0001 | 0.0003 | 0.0009 | 0.0003 | 0.0017 | 0.0006 | 0.0007 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0003 | 0.0009 |
Punctate Nuclear | 0.0047 | 0.0156 | 0.0129 | 0.0048 | 0.0091 | 0.018 | 0.0309 | 0.0441 | 0.0491 | 0.0451 | 0.0912 | 0.0562 | 0.0127 | 0.0177 | 0.0095 | 0.0535 | 0.0061 | 0.0304 |
Vacuole | 0.0025 | 0.004 | 0.0029 | 0.0034 | 0.0031 | 0.0021 | 0.0022 | 0.003 | 0.0016 | 0.0035 | 0.0014 | 0.002 | 0.0029 | 0.0037 | 0.0018 | 0.0026 | 0.0013 | 0.0021 |
Vacuole Periphery | 0.0003 | 0.0007 | 0.0007 | 0.0005 | 0.0006 | 0.0003 | 0.0004 | 0.0004 | 0.0004 | 0.0005 | 0.0004 | 0.0004 | 0.0006 | 0.0005 | 0.0003 | 0.0009 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 18.9963 | 13.0072 | 18.7675 | 15.7956 | 17.2931 | 24.2372 | 14.9781 | 9.8627 | 11.0687 | 14.6119 |
Translational Efficiency | 0.979 | 1.4634 | 0.7755 | 0.8061 | 0.7188 | 0.5755 | 0.9244 | 1.0196 | 0.7704 | 0.8081 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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