ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 3 | 2 | 3 | 4 | 11 |
Bud | 0 | 2 | 1 | 0 | 1 | 2 | 3 | 6 | 8 | 5 | 8 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 0 | 1 | 8 | 16 |
Bud Neck | 0 | 2 | 0 | 1 | 1 | 0 | 2 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 1 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 9 | 1 | 0 | 1 | 0 | 4 | 1 | 2 | 1 | 2 | 1 | 0 | 1 | 8 | 2 | 11 | 4 | 1 | 2 | 2 | 1 | 1 | 6 |
Cytoplasm | 369 | 477 | 224 | 157 | 235 | 168 | 177 | 213 | 252 | 252 | 162 | 172 | 654 | 720 | 875 | 455 | 450 | 282 | 307 | 467 | 184 | 351 | 385 | 316 |
Endoplasmic Reticulum | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 | 1 | 1 | 0 | 17 | 27 | 10 | 1 | 1 | 2 | 2 | 2 | 3 |
Endosome | 0 | 3 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 24 | 19 | 21 | 2 | 4 | 1 | 2 | 5 | 6 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 1 | 0 | 1 | 0 | 0 | 1 | 1 |
Mitochondria | 34 | 9 | 4 | 10 | 21 | 59 | 110 | 122 | 175 | 208 | 187 | 177 | 1 | 2 | 0 | 6 | 9 | 3 | 25 | 20 | 24 | 12 | 12 | 23 |
Nucleus | 2 | 1 | 0 | 1 | 3 | 0 | 1 | 2 | 2 | 7 | 2 | 4 | 1 | 2 | 5 | 7 | 6 | 5 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 4 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 23 | 15 | 22 | 6 | 4 | 5 | 5 | 15 | 20 |
Vac/Vac Membrane | 0 | 3 | 0 | 0 | 0 | 4 | 4 | 10 | 8 | 17 | 3 | 15 | 9 | 18 | 26 | 27 | 90 | 28 | 9 | 8 | 0 | 3 | 2 | 11 |
Unique Cell Count | 384 | 491 | 228 | 161 | 249 | 191 | 243 | 297 | 350 | 374 | 294 | 287 | 664 | 738 | 895 | 516 | 518 | 346 | 381 | 532 | 229 | 397 | 451 | 441 |
Labelled Cell Count | 408 | 510 | 232 | 169 | 265 | 236 | 301 | 360 | 453 | 493 | 374 | 378 | 670 | 749 | 917 | 568 | 632 | 383 | 381 | 532 | 229 | 397 | 451 | 441 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.4 | 4.0 | 3.6 | 3.4 | 3.4 | 3.8 | 3.1 | 3.6 | 3.4 | 3.2 | 3.4 | 3.3 | 5.1 | 5.3 | 5.6 | 6.1 | 7.4 | 6.8 | 3.8 | 4.0 | 4.7 |
Std Deviation (1e-4) | 0.8 | 0.7 | 0.8 | 1.0 | 1.0 | 2.0 | 1.2 | 1.4 | 1.0 | 1.0 | 1.3 | 1.0 | 1.4 | 1.5 | 1.3 | 1.2 | 1.6 | 1.5 | 1.1 | 1.4 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.07 | 0.07 | -0.19 | -0.01 | -0.09 | -0.16 | -0.07 | -0.11 | 0.52 | 0.58 | 0.63 | 0.78 | 1.05 | 0.92 | 0.07 | 0.16 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.5 | 6.7 | 4.4 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8967 | 3.1437 | 3.224 | 3.1897 | 3.1028 | 3.2981 | 2.5557 | 3.2124 | 3.1966 | 3.0412 | 3.1907 | 3.2588 | 3.0459 | 3.3804 | 3.7164 | 3.628 | 3.1641 | 3.3541 |
Actin | 0.0117 | 0.0008 | 0.0123 | 0.0055 | 0.0149 | 0.0074 | 0.0034 | 0.0018 | 0.0061 | 0.0004 | 0.0023 | 0.0023 | 0.0013 | 0.0037 | 0.0007 | 0.0114 | 0.0005 | 0.0012 |
Bud | 0.0051 | 0.0009 | 0.0084 | 0.0251 | 0.0036 | 0.0023 | 0.0027 | 0.004 | 0.0011 | 0.0036 | 0.0171 | 0.0091 | 0.0002 | 0.0029 | 0.001 | 0.0079 | 0.0009 | 0.0005 |
Bud Neck | 0.0019 | 0.0005 | 0.0007 | 0.0005 | 0.003 | 0.0009 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0049 | 0.0033 | 0.0005 | 0.0003 | 0.0003 | 0.0006 | 0.0002 | 0.0005 |
Bud Periphery | 0.0107 | 0.0034 | 0.009 | 0.0218 | 0.0027 | 0.0011 | 0.002 | 0.0009 | 0.0007 | 0.0063 | 0.0088 | 0.0045 | 0.0006 | 0.0011 | 0.0016 | 0.004 | 0.0003 | 0.0005 |
Bud Site | 0.0071 | 0.0176 | 0.0138 | 0.0009 | 0.0029 | 0.0005 | 0.0029 | 0.0066 | 0.0036 | 0.0003 | 0.0278 | 0.0007 | 0.0008 | 0.0154 | 0.009 | 0.0017 | 0.0003 | 0.0001 |
Cell Periphery | 0.0017 | 0.0004 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 |
Cytoplasm | 0.0797 | 0.1033 | 0.1173 | 0.0835 | 0.09 | 0.1529 | 0.1962 | 0.2597 | 0.212 | 0.1496 | 0.1214 | 0.2604 | 0.1334 | 0.1875 | 0.0827 | 0.1349 | 0.1335 | 0.1857 |
Cytoplasmic Foci | 0.0209 | 0.0056 | 0.0165 | 0.0058 | 0.0079 | 0.0201 | 0.0079 | 0.0068 | 0.0082 | 0.0039 | 0.0086 | 0.0075 | 0.0061 | 0.0056 | 0.0103 | 0.0137 | 0.004 | 0.0077 |
Eisosomes | 0.0017 | 0.0002 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0058 | 0.0013 | 0.0015 | 0.0014 | 0.0019 | 0.0014 | 0.0022 | 0.0009 | 0.0014 | 0.0006 | 0.0004 | 0.0012 | 0.0017 | 0.0012 | 0.0015 | 0.0013 | 0.0006 | 0.0009 |
Endosome | 0.0116 | 0.0009 | 0.0035 | 0.0018 | 0.0094 | 0.0028 | 0.0067 | 0.0012 | 0.0017 | 0.0008 | 0.0004 | 0.0011 | 0.002 | 0.0008 | 0.0025 | 0.0052 | 0.0007 | 0.0011 |
Golgi | 0.0049 | 0.0001 | 0.0018 | 0.0003 | 0.0021 | 0.0007 | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0024 | 0 | 0.0001 |
Lipid Particles | 0.0122 | 0.0003 | 0.0036 | 0.0003 | 0.0072 | 0.0018 | 0.0013 | 0.0005 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0004 | 0.0002 | 0.0005 | 0.0069 | 0.0001 | 0.0002 |
Mitochondria | 0.011 | 0.0003 | 0.0015 | 0.0021 | 0.0033 | 0.0011 | 0.0009 | 0.0003 | 0.0004 | 0.0004 | 0.0009 | 0.0004 | 0.0004 | 0.0002 | 0.0006 | 0.0013 | 0.0002 | 0.0005 |
None | 0.7778 | 0.8554 | 0.7945 | 0.842 | 0.7947 | 0.7787 | 0.7514 | 0.7064 | 0.7461 | 0.8231 | 0.7826 | 0.6958 | 0.8421 | 0.768 | 0.8808 | 0.7698 | 0.8356 | 0.7957 |
Nuclear Periphery | 0.0035 | 0.0004 | 0.0011 | 0.0009 | 0.0093 | 0.0035 | 0.0043 | 0.0008 | 0.0015 | 0.0007 | 0.0005 | 0.001 | 0.0012 | 0.0005 | 0.0014 | 0.0031 | 0.0012 | 0.0008 |
Nucleolus | 0.0025 | 0.0007 | 0.0006 | 0.0005 | 0.0039 | 0.0006 | 0.0014 | 0.0005 | 0.0006 | 0.0002 | 0.0013 | 0.0008 | 0.0004 | 0.0003 | 0.0005 | 0.0009 | 0.0006 | 0.0002 |
Nucleus | 0.008 | 0.0036 | 0.0029 | 0.0036 | 0.0255 | 0.0057 | 0.0048 | 0.0041 | 0.0066 | 0.0067 | 0.0054 | 0.0046 | 0.0028 | 0.0038 | 0.0017 | 0.0037 | 0.012 | 0.0013 |
Peroxisomes | 0.0095 | 0.0002 | 0.0038 | 0.0003 | 0.0032 | 0.0024 | 0.0005 | 0.0007 | 0.0004 | 0.0002 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0009 | 0.0024 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0057 | 0.0032 | 0.0051 | 0.0014 | 0.0086 | 0.015 | 0.0081 | 0.0031 | 0.0076 | 0.0022 | 0.0152 | 0.0052 | 0.0045 | 0.0068 | 0.0029 | 0.0273 | 0.0083 | 0.0022 |
Vacuole | 0.0054 | 0.0006 | 0.0013 | 0.0014 | 0.0034 | 0.0007 | 0.0013 | 0.0007 | 0.0008 | 0.0005 | 0.0006 | 0.0009 | 0.0007 | 0.0007 | 0.0007 | 0.0007 | 0.0006 | 0.0003 |
Vacuole Periphery | 0.0016 | 0.0001 | 0.0002 | 0.0002 | 0.0016 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.0191 | 5.6472 | 12.1058 | 12.8643 | 7.2805 | 16.8365 | 11.1025 | 16.2715 | 18.6636 | 9.889 |
Translational Efficiency | 0.6539 | 0.7617 | 0.4914 | 0.7112 | 0.78 | 0.4384 | 0.632 | 0.6357 | 0.638 | 0.5583 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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