Standard name
Human Ortholog
Description Protein of unknown function; required for selective and nonselective autophagy, and mitophagy; regulates the rate of autophagosome formation; interacts with Atg9p, and has a similar peri-mitochondrial localization; elevated Gcn4p-dependent expression under autophagy-inducing conditions; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; ATG41 has a paralog, ICY1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.69 0.76 0.73 0.94 0.96 0.92 0.82 0.81 0.79 0.73 0.62 0.75 0.84 0.85 0.72 0.83 0.88 0.2 0.26 0.24 0.28 0.16 0.13
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0 0 0 0 0 0
Endosome 0 0.09 0 0 0 0 0 0 0 0 0 0.16 0.1 0.08 0.07 0.07 0.06 0.2 0.22 0.21 0.16 0.3 0.31
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.17 0.13 0 0.22 0.32 0.37 0.43 0.48 0.64 0 0 0 0 0 0 0 0 0.07 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.26 0.19 0.16 0 0 0 0 0 0 0 0 0.14 0.14 0.13 0.22 0.15 0.1 0.51 0.42 0.38 0.39 0.37 0.38
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 1 1 3 7
Bud 0 6 10 0 6 5 20 7 8 11 4 8 2 4 0 2 0 4 3 2 2 9 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 2 0 0 0 2
Bud Site 0 0 0 0 0 0 1 0 1 0 2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 2 1 0 1 3 2 1 0 0 1 0 0 3 0 0 0 0 0 0
Cytoplasm 141 243 116 179 238 312 444 277 256 186 226 241 278 300 87 108 68 45 94 46 39 55 49
Endoplasmic Reticulum 8 0 0 3 0 1 0 0 0 0 1 3 0 1 6 8 4 0 3 4 0 1 1
Endosome 6 30 6 4 5 4 6 0 3 0 1 52 33 29 8 9 5 48 77 40 21 105 121
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 1 2 0 0 0 2
Mitochondria 8 3 27 25 6 76 171 127 139 123 231 0 0 3 2 4 2 3 9 13 7 14 6
Nucleus 0 1 1 1 0 0 3 1 1 0 1 0 0 0 2 0 0 2 1 0 3 2 2
Nuclear Periphery 0 2 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 3 3 0 1 3
Nucleolus 0 2 0 0 0 2 2 0 0 4 0 2 0 1 0 0 0 0 0 0 0 3 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 3 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 1 2 3 0 1 0 8 3 3 1 2 16 3 8 2 1 0 3 3 2 1 14 19
Vac/Vac Membrane 54 61 26 1 6 8 9 8 3 5 3 44 48 45 27 20 8 119 150 72 53 129 146
Unique Cell Count 204 319 159 191 247 338 539 342 325 254 363 320 332 355 121 130 77 235 359 191 138 350 388
Labelled Cell Count 219 350 189 213 264 409 664 426 417 332 472 371 364 394 136 152 90 235 359 191 138 350 388


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.1 4.4 4.8 5.0 3.9 3.8 3.5 3.6 4.0 3.2 6.6 6.6 6.6 7.4 7.8 8.4 5.6 6.4 6.5
Std Deviation (1e-4) 0.7 0.9 1.5 1.8 1.8 1.7 1.7 2.0 1.5 2.8 1.2 2.0 2.1 1.8 1.4 2.2 3.1 2.1 2.2 2.2
Intensity Change (Log2) 0.14 0.2 -0.15 -0.18 -0.31 -0.28 -0.13 -0.43 0.59 0.61 0.6 0.76 0.84 0.94 0.35 0.56 0.58


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 -2.0 0 0 0 0 0 0 -2.1 0 0 0 -2.0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5.3 6.9 5.8 2.6 2.0 1.4 0.1 -2.4 0.6 2.8 3.1 -0.2 2.1 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 3.9 2.4 1.8 1.1 1.2 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -5.2 0 0 0 0 0 0 -7.6 -7.7 -7.2 -4.2 -3.8 -3.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1.6 0 0 0 0 0
Vacuole -5.5 -5.1 -5.8 -7.5 -5.8 -6.7 -5.4 -7.1 -0.8 -0.5 -1.1 1.3 -0.2 -1.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2103 3.2377 2.6748 2.5168 2.07 2.6532 2.6556 3.2947 3.0777 2.7777 2.5646 2.9974 4.3211 4.6233 4.4436 4.1888 4.1052 4.1497
Actin 0.0138 0 0.0114 0.0167 0.0063 0.0043 0.0114 0.0001 0.0084 0.0027 0.0037 0.0018 0.0104 0.0001 0.0019 0.0001 0.0669 0.0067
Bud 0.0008 0.0003 0.0024 0.0208 0.0028 0.001 0.0007 0.0003 0.001 0.0065 0.0008 0.0004 0.0006 0.0002 0.0015 0.0003 0.0027 0.0004
Bud Neck 0.0054 0.0006 0.0059 0.0026 0.0006 0.0011 0.002 0.0005 0.0009 0.0008 0.0004 0.0011 0.0017 0.0001 0.0003 0.0008 0.0014 0.0009
Bud Periphery 0.0008 0.0001 0.0018 0.0303 0.0048 0.0006 0.0006 0.0002 0.0006 0.0048 0.0006 0.0003 0.0007 0 0.0009 0.0001 0.0022 0.0002
Bud Site 0.0061 0.0007 0.014 0.0161 0.002 0.0006 0.0075 0.0013 0.0057 0.004 0.0009 0.0004 0.0011 0.0006 0.002 0.0003 0.0144 0.0003
Cell Periphery 0.0003 0.0001 0.0002 0.0007 0.0002 0.0001 0.0003 0.0001 0.0002 0.0006 0.0001 0 0.0001 0 0.0001 0 0.0002 0
Cytoplasm 0.5004 0.7682 0.6184 0.5539 0.4894 0.645 0.6236 0.7998 0.6879 0.5947 0.5499 0.7288 0.5921 0.8187 0.7302 0.535 0.4472 0.6894
Cytoplasmic Foci 0.064 0.0331 0.0538 0.0511 0.1013 0.0765 0.0511 0.0651 0.0728 0.0801 0.0776 0.0662 0.0702 0.0355 0.0589 0.156 0.0624 0.0766
Eisosomes 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0003 0.0006 0.0001 0 0.0001 0 0 0 0.0004 0.0001
Endoplasmic Reticulum 0.0082 0.0036 0.0066 0.0035 0.0025 0.0042 0.0104 0.0056 0.0056 0.0029 0.0048 0.0049 0.012 0.0036 0.0063 0.0013 0.0022 0.004
Endosome 0.2078 0.1085 0.1284 0.1092 0.1789 0.1595 0.1524 0.0411 0.1479 0.1565 0.1969 0.1195 0.1672 0.0506 0.1144 0.1904 0.2314 0.1365
Golgi 0.0159 0.0011 0.0079 0.0049 0.0318 0.005 0.0069 0.0012 0.0053 0.0114 0.0115 0.0033 0.0052 0.0008 0.0026 0.004 0.0261 0.0046
Lipid Particles 0.016 0.0002 0.0053 0.0003 0.0231 0.0088 0.0111 0.0005 0.0024 0.0107 0.0282 0.0016 0.0055 0.0002 0.0007 0.0012 0.0029 0.0017
Mitochondria 0.011 0.0003 0.0222 0.0017 0.0698 0.0059 0.0029 0.0008 0.0024 0.0441 0.0108 0.0029 0.0022 0.0002 0.0009 0.0006 0.0124 0.0016
None 0.0404 0.0284 0.0263 0.1369 0.0251 0.034 0.0438 0.0429 0.0139 0.0303 0.0397 0.0171 0.0627 0.0545 0.0458 0.0108 0.0715 0.0311
Nuclear Periphery 0.01 0.0027 0.0087 0.0024 0.0028 0.0047 0.0126 0.0039 0.0043 0.0039 0.0055 0.004 0.0124 0.0029 0.0044 0.0017 0.0018 0.0066
Nucleolus 0.0008 0.0001 0.0004 0.0003 0.0007 0.0003 0.0004 0.0003 0.0005 0.0004 0.0003 0.0001 0.0005 0.0001 0.0002 0.0001 0.0002 0.0002
Nucleus 0.0053 0.0027 0.0049 0.0041 0.0032 0.0054 0.0111 0.0054 0.0038 0.0049 0.004 0.0049 0.0058 0.004 0.0043 0.0023 0.0022 0.0053
Peroxisomes 0.0098 0.0001 0.0213 0.0013 0.0122 0.0058 0.0044 0.0031 0.0061 0.0109 0.0121 0.008 0.0019 0.0001 0.001 0.0136 0.0072 0.0042
Punctate Nuclear 0.006 0.0004 0.0039 0.0009 0.0026 0.003 0.0021 0.0014 0.0018 0.0027 0.0052 0.0012 0.0033 0.0015 0.0022 0.001 0.0014 0.0021
Vacuole 0.0647 0.0428 0.0442 0.0329 0.0302 0.0276 0.0394 0.0233 0.0241 0.0204 0.0378 0.0263 0.039 0.0204 0.017 0.0677 0.0334 0.0216
Vacuole Periphery 0.0123 0.0059 0.012 0.0094 0.0096 0.0066 0.005 0.0029 0.0041 0.0057 0.0091 0.0072 0.0052 0.0058 0.0044 0.0126 0.0094 0.0062

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.6396 33.5992 33.8364 37.8676 48.7313 64.3967 92.8166 97.4477 73.0945 70.5092
Translational Efficiency 1.6345 2.2743 1.5969 1.4492 1.1345 2.6834 1.6701 1.7148 2.0614 1.756

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of unknown function; required for selective and nonselective autophagy, and mitophagy; regulates the rate of autophagosome formation; interacts with Atg9p, and has a similar peri-mitochondrial localization; elevated Gcn4p-dependent expression under autophagy-inducing conditions; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; ATG41 has a paralog, ICY1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (19%), vacuole (11%), mixed (31%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Atg41

Atg41


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg41-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available