Standard name | |
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Human Ortholog | |
Description | Protein of unknown function; required for selective and nonselective autophagy, and mitophagy; regulates the rate of autophagosome formation; interacts with Atg9p, and has a similar peri-mitochondrial localization; elevated Gcn4p-dependent expression under autophagy-inducing conditions; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; ATG41 has a paralog, ICY1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 7 |
Bud | 0 | 6 | 10 | 0 | 6 | 5 | – | 20 | 7 | 8 | 11 | 4 | 8 | 2 | 4 | 0 | 2 | 0 | 4 | 3 | 2 | 2 | 9 | 14 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 2 | 1 | – | 0 | 1 | 3 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 141 | 243 | 116 | 179 | 238 | 312 | – | 444 | 277 | 256 | 186 | 226 | 241 | 278 | 300 | 87 | 108 | 68 | 45 | 94 | 46 | 39 | 55 | 49 |
Endoplasmic Reticulum | 8 | 0 | 0 | 3 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 6 | 8 | 4 | 0 | 3 | 4 | 0 | 1 | 1 |
Endosome | 6 | 30 | 6 | 4 | 5 | 4 | – | 6 | 0 | 3 | 0 | 1 | 52 | 33 | 29 | 8 | 9 | 5 | 48 | 77 | 40 | 21 | 105 | 121 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 2 |
Mitochondria | 8 | 3 | 27 | 25 | 6 | 76 | – | 171 | 127 | 139 | 123 | 231 | 0 | 0 | 3 | 2 | 4 | 2 | 3 | 9 | 13 | 7 | 14 | 6 |
Nucleus | 0 | 1 | 1 | 1 | 0 | 0 | – | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 0 | 3 | 2 | 2 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 1 | 3 |
Nucleolus | 0 | 2 | 0 | 0 | 0 | 2 | – | 2 | 0 | 0 | 4 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 2 | 3 | 0 | 1 | 0 | – | 8 | 3 | 3 | 1 | 2 | 16 | 3 | 8 | 2 | 1 | 0 | 3 | 3 | 2 | 1 | 14 | 19 |
Vac/Vac Membrane | 54 | 61 | 26 | 1 | 6 | 8 | – | 9 | 8 | 3 | 5 | 3 | 44 | 48 | 45 | 27 | 20 | 8 | 119 | 150 | 72 | 53 | 129 | 146 |
Unique Cell Count | 204 | 319 | 159 | 191 | 247 | 338 | 539 | 342 | 325 | 254 | 363 | 320 | 332 | 355 | 121 | 130 | 77 | 235 | 359 | 191 | 138 | 350 | 388 | |
Labelled Cell Count | 219 | 350 | 189 | 213 | 264 | 409 | 664 | 426 | 417 | 332 | 472 | 371 | 364 | 394 | 136 | 152 | 90 | 235 | 359 | 191 | 138 | 350 | 388 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.1 | 4.4 | 4.8 | 5.0 | 3.9 | – | 3.8 | 3.5 | 3.6 | 4.0 | 3.2 | 6.6 | 6.6 | 6.6 | 7.4 | 7.8 | 8.4 | 5.6 | 6.4 | 6.5 |
Std Deviation (1e-4) | 0.7 | 0.9 | 1.5 | 1.8 | 1.8 | 1.7 | – | 1.7 | 2.0 | 1.5 | 2.8 | 1.2 | 2.0 | 2.1 | 1.8 | 1.4 | 2.2 | 3.1 | 2.1 | 2.2 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.14 | 0.2 | -0.15 | – | -0.18 | -0.31 | -0.28 | -0.13 | -0.43 | 0.59 | 0.61 | 0.6 | 0.76 | 0.84 | 0.94 | 0.35 | 0.56 | 0.58 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -2.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | -2.1 | 0 | 0 | 0 | -2.0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5.3 | 6.9 | 5.8 | – | 2.6 | 2.0 | 1.4 | 0.1 | -2.4 | 0.6 | 2.8 | 3.1 | -0.2 | 2.1 | 2.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.9 | 2.4 | 1.8 | 1.1 | 1.2 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -5.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | -7.6 | -7.7 | -7.2 | -4.2 | -3.8 | -3.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -5.5 | -5.1 | -5.8 | – | -7.5 | -5.8 | -6.7 | -5.4 | -7.1 | -0.8 | -0.5 | -1.1 | 1.3 | -0.2 | -1.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2103 | 3.2377 | 2.6748 | 2.5168 | 2.07 | 2.6532 | 2.6556 | 3.2947 | 3.0777 | 2.7777 | 2.5646 | 2.9974 | 4.3211 | 4.6233 | 4.4436 | 4.1888 | 4.1052 | 4.1497 |
Actin | 0.0138 | 0 | 0.0114 | 0.0167 | 0.0063 | 0.0043 | 0.0114 | 0.0001 | 0.0084 | 0.0027 | 0.0037 | 0.0018 | 0.0104 | 0.0001 | 0.0019 | 0.0001 | 0.0669 | 0.0067 |
Bud | 0.0008 | 0.0003 | 0.0024 | 0.0208 | 0.0028 | 0.001 | 0.0007 | 0.0003 | 0.001 | 0.0065 | 0.0008 | 0.0004 | 0.0006 | 0.0002 | 0.0015 | 0.0003 | 0.0027 | 0.0004 |
Bud Neck | 0.0054 | 0.0006 | 0.0059 | 0.0026 | 0.0006 | 0.0011 | 0.002 | 0.0005 | 0.0009 | 0.0008 | 0.0004 | 0.0011 | 0.0017 | 0.0001 | 0.0003 | 0.0008 | 0.0014 | 0.0009 |
Bud Periphery | 0.0008 | 0.0001 | 0.0018 | 0.0303 | 0.0048 | 0.0006 | 0.0006 | 0.0002 | 0.0006 | 0.0048 | 0.0006 | 0.0003 | 0.0007 | 0 | 0.0009 | 0.0001 | 0.0022 | 0.0002 |
Bud Site | 0.0061 | 0.0007 | 0.014 | 0.0161 | 0.002 | 0.0006 | 0.0075 | 0.0013 | 0.0057 | 0.004 | 0.0009 | 0.0004 | 0.0011 | 0.0006 | 0.002 | 0.0003 | 0.0144 | 0.0003 |
Cell Periphery | 0.0003 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Cytoplasm | 0.5004 | 0.7682 | 0.6184 | 0.5539 | 0.4894 | 0.645 | 0.6236 | 0.7998 | 0.6879 | 0.5947 | 0.5499 | 0.7288 | 0.5921 | 0.8187 | 0.7302 | 0.535 | 0.4472 | 0.6894 |
Cytoplasmic Foci | 0.064 | 0.0331 | 0.0538 | 0.0511 | 0.1013 | 0.0765 | 0.0511 | 0.0651 | 0.0728 | 0.0801 | 0.0776 | 0.0662 | 0.0702 | 0.0355 | 0.0589 | 0.156 | 0.0624 | 0.0766 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0.0001 |
Endoplasmic Reticulum | 0.0082 | 0.0036 | 0.0066 | 0.0035 | 0.0025 | 0.0042 | 0.0104 | 0.0056 | 0.0056 | 0.0029 | 0.0048 | 0.0049 | 0.012 | 0.0036 | 0.0063 | 0.0013 | 0.0022 | 0.004 |
Endosome | 0.2078 | 0.1085 | 0.1284 | 0.1092 | 0.1789 | 0.1595 | 0.1524 | 0.0411 | 0.1479 | 0.1565 | 0.1969 | 0.1195 | 0.1672 | 0.0506 | 0.1144 | 0.1904 | 0.2314 | 0.1365 |
Golgi | 0.0159 | 0.0011 | 0.0079 | 0.0049 | 0.0318 | 0.005 | 0.0069 | 0.0012 | 0.0053 | 0.0114 | 0.0115 | 0.0033 | 0.0052 | 0.0008 | 0.0026 | 0.004 | 0.0261 | 0.0046 |
Lipid Particles | 0.016 | 0.0002 | 0.0053 | 0.0003 | 0.0231 | 0.0088 | 0.0111 | 0.0005 | 0.0024 | 0.0107 | 0.0282 | 0.0016 | 0.0055 | 0.0002 | 0.0007 | 0.0012 | 0.0029 | 0.0017 |
Mitochondria | 0.011 | 0.0003 | 0.0222 | 0.0017 | 0.0698 | 0.0059 | 0.0029 | 0.0008 | 0.0024 | 0.0441 | 0.0108 | 0.0029 | 0.0022 | 0.0002 | 0.0009 | 0.0006 | 0.0124 | 0.0016 |
None | 0.0404 | 0.0284 | 0.0263 | 0.1369 | 0.0251 | 0.034 | 0.0438 | 0.0429 | 0.0139 | 0.0303 | 0.0397 | 0.0171 | 0.0627 | 0.0545 | 0.0458 | 0.0108 | 0.0715 | 0.0311 |
Nuclear Periphery | 0.01 | 0.0027 | 0.0087 | 0.0024 | 0.0028 | 0.0047 | 0.0126 | 0.0039 | 0.0043 | 0.0039 | 0.0055 | 0.004 | 0.0124 | 0.0029 | 0.0044 | 0.0017 | 0.0018 | 0.0066 |
Nucleolus | 0.0008 | 0.0001 | 0.0004 | 0.0003 | 0.0007 | 0.0003 | 0.0004 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0002 |
Nucleus | 0.0053 | 0.0027 | 0.0049 | 0.0041 | 0.0032 | 0.0054 | 0.0111 | 0.0054 | 0.0038 | 0.0049 | 0.004 | 0.0049 | 0.0058 | 0.004 | 0.0043 | 0.0023 | 0.0022 | 0.0053 |
Peroxisomes | 0.0098 | 0.0001 | 0.0213 | 0.0013 | 0.0122 | 0.0058 | 0.0044 | 0.0031 | 0.0061 | 0.0109 | 0.0121 | 0.008 | 0.0019 | 0.0001 | 0.001 | 0.0136 | 0.0072 | 0.0042 |
Punctate Nuclear | 0.006 | 0.0004 | 0.0039 | 0.0009 | 0.0026 | 0.003 | 0.0021 | 0.0014 | 0.0018 | 0.0027 | 0.0052 | 0.0012 | 0.0033 | 0.0015 | 0.0022 | 0.001 | 0.0014 | 0.0021 |
Vacuole | 0.0647 | 0.0428 | 0.0442 | 0.0329 | 0.0302 | 0.0276 | 0.0394 | 0.0233 | 0.0241 | 0.0204 | 0.0378 | 0.0263 | 0.039 | 0.0204 | 0.017 | 0.0677 | 0.0334 | 0.0216 |
Vacuole Periphery | 0.0123 | 0.0059 | 0.012 | 0.0094 | 0.0096 | 0.0066 | 0.005 | 0.0029 | 0.0041 | 0.0057 | 0.0091 | 0.0072 | 0.0052 | 0.0058 | 0.0044 | 0.0126 | 0.0094 | 0.0062 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 45.6396 | 33.5992 | 33.8364 | 37.8676 | 48.7313 | 64.3967 | 92.8166 | 97.4477 | 73.0945 | 70.5092 |
Translational Efficiency | 1.6345 | 2.2743 | 1.5969 | 1.4492 | 1.1345 | 2.6834 | 1.6701 | 1.7148 | 2.0614 | 1.756 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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