Standard name
Human Ortholog
Description Conserved fungal gene linked to DNA repair and proteasome function; putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 1.0 0.99 0.99 0.97 0.98 0.9 0.85 0.85 0.82 0.82 0.84 0.98 0.98 0.97 0.92 0.94 0.96 0.92 0.98 0.96 0.93 0.94 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.06 0.05 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.05 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0.09 0.07 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 6 1 2 1 3 0 0 0 0 0 0 0 0 1 0 1 1
Bud 0 0 1 0 0 1 1 2 0 0 0 1 7 4 4 0 0 0 2 1 1 4 2 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 10 10 1 4 3 9 9 17 19 12 3 7 12 17 22 1 8 8 0 0 0 0 0 0
Cytoplasm 465 545 412 249 372 376 440 439 255 272 71 109 643 646 750 519 368 338 380 527 393 488 554 571
Endoplasmic Reticulum 0 1 1 0 7 6 25 29 16 20 6 4 8 3 11 12 13 13 12 1 2 3 3 9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 3
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 1 1 0 0 0 0 0 1
Mitochondria 0 1 2 1 0 2 5 7 17 17 10 10 0 0 0 3 4 3 0 0 0 1 0 1
Nucleus 2 0 0 0 2 3 6 7 3 5 0 5 4 5 8 10 7 2 2 2 0 6 6 1
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 1 0
Nucleolus 0 0 0 0 0 1 0 1 0 1 0 0 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 4 0 1 2 1 2 10 33 5 18 1 3 0 2 2 52 26 18 3 0 1 17 12 15
Unique Cell Count 472 546 415 252 383 384 487 519 301 330 87 130 657 658 770 563 391 351 414 538 410 527 589 618
Labelled Cell Count 481 557 418 257 385 400 496 542 316 347 92 143 674 679 798 599 427 383 414 538 410 527 589 618


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.6 27.9 18.5 18.2 19.5 18.6 17.9 16.6 17.3 14.9 16.0 15.6 28.5 29.1 28.5 21.8 29.5 25.9 19.5 21.1 21.9
Std Deviation (1e-4) 4.2 3.9 3.4 3.9 3.9 3.5 4.0 4.1 4.2 3.9 4.1 4.3 5.7 6.7 6.3 9.3 10.4 9.6 4.3 4.6 5.0
Intensity Change (Log2) – – – -0.02 0.07 0.0 -0.05 -0.16 -0.1 -0.32 -0.22 -0.25 0.62 0.65 0.62 0.23 0.67 0.48 0.07 0.19 0.24


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 3.4 4.9 3.5 0 0 2.3 2.9 3.1 0 0 0
Cytoplasm -0.6 -2.3 -1.6 -5.8 -7.8 -7.6 -8.3 -7.9 -7.5 -1.8 -1.5 -2.2 -5.1 -4.1 -2.9
Endoplasmic Reticulum 0 0 0 4.4 4.6 4.4 4.8 0 0 0 0 0 2.6 3.3 3.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.2 4.0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 5.0 0 4.5 0 0 0 0 0 6.1 5.1 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 25.6561 27.4194 26.6453 24.8926 24.8142 25.6485 14.8753 21.9155 19.1629 16.3759 16.2896 20.3988 20.4174 25.3229 23.6017 22.0868 19.4941 23.6998
Actin 0.0021 0.0003 0.0002 0.0001 0.0001 0.0003 0.0403 0.0026 0.0194 0.0097 0.062 0.0091 0.0132 0.0008 0.0087 0.0007 0.0375 0.0015
Bud 0.0002 0 0.0001 0.0001 0 0.0001 0.0023 0.0074 0.001 0.0008 0.0003 0.0002 0.0007 0 0.0003 0.0001 0.0004 0
Bud Neck 0.0005 0.0002 0.0003 0.0004 0.0004 0.001 0.0045 0.0002 0.0009 0.0015 0.0015 0.0012 0.0009 0.0002 0.0004 0.0004 0.0013 0.0008
Bud Periphery 0.0002 0 0 0 0 0.0001 0.0027 0.002 0.0006 0.0021 0.0001 0.0001 0.0003 0 0.0002 0 0.0004 0
Bud Site 0.0002 0.0001 0.0001 0 0 0.0001 0.0098 0.003 0.0145 0.003 0.0014 0.0001 0.002 0.0002 0.0017 0.0004 0.0014 0
Cell Periphery 0.0001 0 0 0 0 0 0.0009 0.0003 0.0004 0.0044 0 0 0.0002 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.9531 0.9902 0.9883 0.9769 0.9888 0.969 0.6798 0.9707 0.9066 0.7479 0.6899 0.9669 0.8827 0.9854 0.9559 0.9556 0.8298 0.9716
Cytoplasmic Foci 0.002 0.0003 0.0005 0.0005 0.0003 0.002 0.0415 0.003 0.0157 0.0184 0.058 0.0045 0.014 0.0043 0.014 0.0009 0.0294 0.0014
Eisosomes 0 0 0 0 0 0 0.0004 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0016 0.0007 0.0005 0.0016 0.0004 0.0024 0.0208 0.0004 0.002 0.011 0.0037 0.0023 0.0066 0.0006 0.0003 0.0008 0.0029 0.0019
Endosome 0.002 0.0001 0.0005 0.0006 0.0002 0.0018 0.0659 0.0001 0.0104 0.0854 0.0908 0.003 0.019 0.0002 0.0039 0.0009 0.0217 0.0035
Golgi 0.0003 0 0 0 0 0.0002 0.0123 0.0001 0.0031 0.0323 0.0155 0.0027 0.0041 0.0002 0.0014 0.0001 0.0062 0.0013
Lipid Particles 0.001 0 0 0 0 0 0.0174 0.0001 0.0041 0.0079 0.0223 0.0003 0.0026 0.0012 0.0012 0 0.0139 0.0021
Mitochondria 0.0009 0 0 0.0001 0 0.0005 0.0135 0.0029 0.0032 0.0417 0.0012 0.0003 0.0021 0.0001 0.0016 0.0004 0.0065 0.0009
None 0.0014 0.0002 0.0002 0.0005 0.0002 0.0004 0.0155 0.0025 0.0046 0.0009 0.0014 0.0017 0.0068 0.0004 0.0008 0.004 0.006 0.0004
Nuclear Periphery 0.0039 0.0013 0.0011 0.0038 0.0014 0.0036 0.0241 0.0004 0.0017 0.0051 0.0014 0.0011 0.0105 0.0005 0.0005 0.0038 0.0124 0.0035
Nucleolus 0.0008 0 0 0 0 0 0.0024 0.0002 0.0001 0.0001 0.0001 0 0.0007 0 0.0003 0.0005 0.0007 0
Nucleus 0.02 0.0062 0.0076 0.0141 0.0076 0.0168 0.014 0.0026 0.0042 0.0035 0.0035 0.0044 0.0167 0.0021 0.0042 0.0259 0.0163 0.009
Peroxisomes 0.0018 0 0 0 0 0.0001 0.0045 0.0003 0.0009 0.0029 0.0404 0.0002 0.0034 0.0005 0.0022 0.0001 0.0063 0.0006
Punctate Nuclear 0.001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0143 0.0005 0.0036 0.0011 0.0031 0.0016 0.0052 0.0031 0.0004 0.0048 0.003 0.0007
Vacuole 0.0051 0.0002 0.0004 0.0009 0.0004 0.0011 0.0103 0.0005 0.0024 0.0166 0.0027 0.0003 0.0061 0.0001 0.0016 0.0003 0.0021 0.0003
Vacuole Periphery 0.0019 0 0.0001 0.0001 0.0001 0.0002 0.0029 0.0001 0.0004 0.0035 0.0006 0.0001 0.002 0 0.0004 0.0001 0.0015 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 38.979 39.9077 33.5912 47.6799 38.0114 41.9541 41.8563 43.6963 47.3157 48.4249
Translational Efficiency 1.8735 1.9527 2.1358 1.947 1.9497 2.2032 1.9939 2.1289 2.0866 1.7374

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Conserved fungal gene linked to DNA repair and proteasome function; putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Cub1

Cub1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cub1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available