Standard name
Human Ortholog
Description Fumarase; converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.15 0.19 0.1 0.07 0.05 0 0 0.06 0 0 0.05 0.07 0.08 0 0.13 0.13 0.26 0.06 0.06 0.09 0.16 0.11 0.11
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.11 0.06 0.1 0 0 0 0 0 0
Cytoplasm 0 0.13 0 0.06 0.07 0.08 0.07 0.06 0.07 0 0 0 0.1 0.18 0.21 0.07 0.07 0 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0.07 0 0 0 0 0 0.12 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0
Endosome 0.05 0.13 0.08 0.06 0.06 0 0.05 0 0 0 0 0 0.06 0.07 0.07 0.06 0 0 0.1 0.16 0.13 0.09 0.11 0.11
Golgi 0.4 0.32 0.49 0.59 0.5 0.37 0.23 0.31 0.17 0.12 0.15 0.14 0.51 0.56 0.55 0.63 0.7 0.76 0.29 0.19 0.33 0.3 0.26 0.25
Mitochondria 0.62 0.34 0.46 0.57 0.41 0.63 0.57 0.62 0.7 0.81 0.81 0.84 0.4 0.15 0.18 0.1 0.11 0.1 0.37 0.35 0.31 0.22 0.27 0.24
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.06 0.08 0.07 0.12 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.26 0.14 0.08 0.19 0.12 0.18 0.14 0.08 0.06 0.1 0.07 0.16 0.12 0.12 0.16 0.19 0.11 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 8 38 15 14 9 9 11 12 10 4 14 6 19 4 39 47 92 12 6 21 9 23 23
Bud 4 1 5 5 0 5 4 7 4 2 8 8 1 3 1 7 4 8 4 5 2 0 9 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 4
Bud Site 0 0 0 0 0 0 0 1 1 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 5 2 3 2 4 1 3 3 1 5 1 15 6 34 23 35 0 1 0 0 0 1
Cytoplasm 6 7 9 10 15 15 19 17 14 9 9 7 8 45 32 21 27 13 1 3 1 3 6 3
Endoplasmic Reticulum 4 2 14 3 9 6 11 5 22 4 3 8 5 15 9 6 12 14 6 1 4 0 3 7
Endosome 8 7 16 9 12 8 13 9 2 3 2 2 5 17 10 18 14 14 19 16 30 5 23 23
Golgi 68 17 98 92 104 72 66 84 33 32 35 40 41 140 84 190 254 271 59 20 79 18 58 51
Mitochondria 107 18 93 89 86 122 160 170 133 211 194 233 32 37 27 31 40 35 77 37 74 13 58 49
Nucleus 0 0 0 0 0 0 4 0 1 1 1 0 1 1 0 2 0 1 0 0 1 0 0 0
Nuclear Periphery 0 0 1 2 4 1 6 4 4 7 11 5 1 0 0 1 3 0 2 0 2 0 1 1
Nucleolus 3 0 2 4 7 9 18 21 13 32 25 28 1 1 1 5 12 9 0 1 0 1 2 1
Peroxisomes 3 0 3 3 1 1 1 0 1 0 0 0 0 6 2 1 3 0 5 1 4 1 5 4
SpindlePole 0 1 0 3 2 1 2 3 0 1 1 0 0 0 1 12 7 7 2 0 1 0 4 2
Vac/Vac Membrane 30 14 29 12 39 23 50 39 15 16 24 18 13 31 19 47 68 41 6 6 9 1 7 12
Unique Cell Count 172 53 201 157 208 195 281 274 190 262 240 276 81 252 153 302 363 357 206 107 239 63 221 208
Labelled Cell Count 247 76 313 249 296 274 367 372 258 332 320 368 115 330 196 414 514 540 206 107 239 63 221 208


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.2 12.1 13.4 12.8 15.0 14.6 13.3 13.8 14.3 13.6 14.0 14.3 14.3 14.7 14.3 15.0 18.2 19.4 10.9 13.5 14.2
Std Deviation (1e-4) 5.2 6.0 4.4 4.2 5.1 5.1 5.9 4.4 4.9 4.5 5.0 5.2 5.4 5.4 5.5 4.2 7.2 5.3 5.3 12.0 5.3
Intensity Change (Log2) – – – -0.07 0.16 0.12 -0.01 0.04 0.09 0.02 0.06 0.09 0.09 0.13 0.09 0.16 0.44 0.53 -0.31 0.01 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.5 -3.7 -4.4 -5.7 -5.3 -3.7 -5.3 -6.1 -4.8 -2.4 -3.6 -4.7 -1.8 -1.9 1.8
Bud 0 0 0 0 0 0 0 0.5 0.3 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1.8 0 3.6 2.0 3.2
Cytoplasm 0.8 1.2 1.3 1.1 0.8 1.2 -0.6 -0.4 -1.2 0 4.4 4.8 1.1 1.4 -0.5
Endoplasmic Reticulum -2.2 -1.2 -1.8 -1.5 -2.8 1.6 -3.0 -3.1 -2.1 -0.2 -0.4 -0.4 -2.8 -2.0 -1.6
Endosome -0.8 -0.9 -1.6 -1.5 -2.3 -3.3 -3.7 -3.8 -4.1 -0.5 -0.5 -0.5 -0.9 -2.1 -2.0
Golgi 1.9 0.3 -2.4 -5.8 -4.0 -6.6 -8.7 -7.8 -8.1 0.3 1.4 1.1 3.1 5.0 6.5
Mitochondria 2.0 -1.0 3.3 2.3 3.4 4.7 7.7 7.6 8.8 -1.0 -7.4 -5.6 -9.2 -9.4 -9.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2.6 0 0 0 0 0 0 0
Nucleolus 0 0 2.2 2.9 3.3 3.0 4.6 4.1 4.1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.0 1.2 -0.8 1.0 -0.1 -2.0 -3.0 -1.4 -2.9 0.3 -0.7 -0.5 0.3 1.3 -1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.0656 16.1031 15.511 15.5643 13.7537 16.4829 12.9914 16.5353 14.3986 14.4616 12.9088 15.4804 15.787 18.6414 17.6341 14.1238 12.5723 17.5565
Actin 0.0232 0.0374 0.0352 0.0147 0.049 0.0081 0.0958 0.0632 0.0315 0.0133 0.0974 0.0437 0.0577 0.0599 0.0322 0.02 0.0536 0.0277
Bud 0.0015 0.0009 0.0029 0.009 0.0041 0.0004 0.0015 0.0022 0.0012 0.0081 0.0143 0.0003 0.0048 0.01 0.0093 0.0115 0.0017 0.0015
Bud Neck 0.0005 0.0008 0.0008 0.0002 0.0012 0.0001 0.0013 0.0009 0.0008 0.0002 0.0007 0.0004 0.001 0.0004 0.0006 0.0006 0.0005 0.0037
Bud Periphery 0.0022 0.0007 0.0049 0.0225 0.0108 0.0013 0.0024 0.0026 0.0019 0.0302 0.0241 0.0006 0.0048 0.0117 0.0076 0.0048 0.0025 0.0019
Bud Site 0.0027 0.0064 0.0022 0.0035 0.0014 0.0002 0.0053 0.0162 0.0051 0.0013 0.0065 0.0003 0.0038 0.0185 0.005 0.0018 0.0017 0.0012
Cell Periphery 0.0005 0.0003 0.0004 0.0019 0.0007 0.0004 0.0008 0.0006 0.0008 0.0006 0.0004 0.0002 0.0006 0.0006 0.0007 0.0005 0.0003 0.0003
Cytoplasm 0.003 0.0005 0.0027 0.0004 0.0012 0.0002 0.0035 0.0046 0.0009 0.0004 0.0005 0.0003 0.0059 0.004 0.0046 0.01 0.0022 0.0063
Cytoplasmic Foci 0.0158 0.0148 0.007 0.0018 0.0134 0.0041 0.017 0.0155 0.0131 0.0036 0.0186 0.0034 0.037 0.0259 0.0191 0.0577 0.0254 0.0088
Eisosomes 0.0008 0.0007 0.0012 0.0006 0.0008 0.0005 0.0023 0.0007 0.003 0.0005 0.0009 0.0004 0.0013 0.0006 0.0016 0.0005 0.0006 0.0004
Endoplasmic Reticulum 0.0022 0.0003 0.0013 0.0004 0.0028 0.0012 0.002 0.0007 0.0006 0.0005 0.0005 0.0009 0.0047 0.0003 0.0005 0.0003 0.0014 0.0012
Endosome 0.0465 0.0351 0.0285 0.0143 0.0748 0.0286 0.0502 0.0542 0.038 0.0198 0.029 0.028 0.0728 0.0441 0.0439 0.0439 0.1121 0.0308
Golgi 0.1156 0.1906 0.0973 0.0328 0.295 0.1593 0.1672 0.2286 0.1299 0.0521 0.1294 0.1705 0.1533 0.1697 0.1453 0.0879 0.2191 0.1729
Lipid Particles 0.0156 0.0061 0.0105 0.0022 0.0048 0.008 0.0298 0.0057 0.0093 0.0033 0.0141 0.0033 0.0267 0.0062 0.011 0.0068 0.0134 0.0124
Mitochondria 0.7413 0.682 0.7736 0.8711 0.503 0.7673 0.5603 0.5825 0.7258 0.8275 0.6283 0.7363 0.578 0.6143 0.6915 0.7236 0.5145 0.7163
None 0.004 0.0001 0.0003 0.0001 0.0017 0.0001 0.0122 0.0001 0.0003 0.0001 0.0003 0.0001 0.0085 0.0002 0.0026 0.0008 0.0004 0.0003
Nuclear Periphery 0.0019 0.0001 0.0017 0.0001 0.0022 0.0011 0.0045 0.0003 0.0004 0.0022 0.0003 0.0003 0.0042 0.0001 0.0003 0.0002 0.0031 0.0003
Nucleolus 0.0003 0.0004 0.0001 0.0001 0.0002 0.0001 0.0029 0.0002 0.0006 0.0003 0.0002 0 0.0043 0.0011 0.0016 0.0008 0.0002 0.0005
Nucleus 0.0005 0.0001 0.0004 0.0002 0.0007 0.0001 0.0156 0.0002 0.0002 0.0015 0.0002 0.0001 0.0017 0.0014 0.001 0.0008 0.0005 0.0003
Peroxisomes 0.0039 0.0095 0.0093 0.0025 0.0018 0.0011 0.0114 0.0074 0.0148 0.0047 0.0279 0.0023 0.0125 0.0183 0.0109 0.0197 0.0088 0.0059
Punctate Nuclear 0.0006 0 0.0003 0 0.0008 0 0.0013 0 0.0001 0 0.0006 0 0.0058 0.0001 0.0004 0.0009 0.0017 0.0001
Vacuole 0.0044 0.0018 0.0037 0.0048 0.0113 0.0032 0.0053 0.0054 0.0093 0.0123 0.0023 0.0018 0.0048 0.0042 0.0043 0.0037 0.0189 0.002
Vacuole Periphery 0.013 0.0115 0.0155 0.0168 0.0182 0.0147 0.0076 0.0082 0.0123 0.0175 0.0036 0.0068 0.0058 0.0085 0.006 0.003 0.0174 0.0052

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 156.4473 143.8885 138.2042 130.3842 123.8499 158.6158 156.193 134.9006 143.3489 147.9558
Translational Efficiency 1.2393 1.2573 1.1337 1.1989 1.273 1.1657 1.1811 1.3405 1.302 1.2014

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Fumarase; converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
Localization
Cell Percentages mitochondrion (68%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Fum1

Fum1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Fum1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available