Standard name
Human Ortholog
Description Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.05 0.06 0.06
Bud 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.11 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.19 0.19 0.14 0 0 0 0 – 0.06 0.05 0.12 0.14 0.13 0.11 0.08 0.17 0.16 0 0.05 0.09 0 0 0
Cytoplasm 0.11 0.2 0.06 0.15 0.12 0.11 0.19 0.18 – 0.27 0.31 0.25 0 0.05 0.06 0.22 0.16 0.06 0.16 0.24 0.07 0 0 0
Endoplasmic Reticulum 0.77 0.62 0.67 0.63 0.6 0.43 0.31 0.32 – 0.27 0.25 0.31 0.35 0.26 0.15 0.07 0.11 0.08 0.6 0.56 0.64 0.53 0.58 0.55
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06
Golgi 0 0 0.05 0 0 0 0 0 – 0 0 0 0.38 0.44 0.45 0.12 0.08 0.06 0.07 0 0 0.11 0.13 0.13
Mitochondria 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0.16 0.16 0 0 0 0 0 0
Nucleus 0 0 0 0 0.07 0.16 0.28 0.27 – 0.14 0.11 0.11 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0 0 0.12 0.09 0.12 0.07 0.07 – 0.05 0.08 0.05 0.12 0.14 0.13 0.25 0.27 0.33 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0.12 0.14 0.15 – 0.23 0.23 0.18 0 0.05 0.16 0.18 0.18 0.25 0.08 0 0 0.15 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 2 0 0 0 1 1 – 2 0 1 10 16 19 2 3 2 7 8 20 18 23 30
Bud 5 4 12 2 2 4 3 3 – 4 7 6 14 14 8 12 15 4 0 0 3 9 6 9
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 2 2 3
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 21 43 20 3 7 5 5 – 15 11 33 80 81 75 15 22 10 2 8 30 4 2 4
Cytoplasm 19 22 13 22 16 20 39 39 – 66 70 68 25 28 40 39 21 4 45 42 23 8 5 1
Endoplasmic Reticulum 133 69 153 92 82 74 63 69 – 65 57 84 198 162 105 12 15 5 174 97 216 200 235 274
Endosome 0 0 0 1 0 0 0 0 – 0 0 0 0 0 2 2 0 0 3 1 1 11 11 30
Golgi 1 2 12 0 1 1 1 0 – 1 3 0 214 270 311 21 11 4 19 3 14 40 52 65
Mitochondria 0 1 0 0 0 2 2 2 – 9 3 5 5 11 5 18 21 10 2 3 8 7 7 17
Nucleus 0 0 0 0 10 28 58 58 – 33 26 30 1 1 4 8 1 2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 19 5 10 17 12 21 15 15 – 13 18 13 69 89 92 45 36 21 1 0 0 0 1 1
Peroxisomes 0 0 1 0 0 0 0 0 – 0 0 0 0 1 1 0 0 0 0 0 0 6 8 5
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 12 19 34 3 2 0 2 1 0 5 0 1
Vac/Vac Membrane 0 0 0 2 12 21 28 33 – 57 53 48 23 29 108 31 23 16 23 3 10 55 40 43
Unique Cell Count 173 112 230 147 136 174 204 216 243 229 268 564 617 691 177 131 63 291 175 336 376 407 499
Labelled Cell Count 182 125 246 156 138 178 215 225 265 248 288 651 721 804 208 170 78 291 175 336 376 407 499


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 157.5 125.1 121.8 148.5 177.2 167.3 161.6 161.6 – 149.0 143.7 152.3 65.2 51.7 42.5 77.2 81.2 97.0 94.6 109.3 115.6
Std Deviation (1e-4) 35.1 26.8 34.7 47.0 52.6 63.6 62.8 62.8 – 55.0 50.7 47.2 17.2 13.2 11.4 35.6 37.3 42.8 31.7 35.6 36.1
Intensity Change (Log2) – – – 0.29 0.54 0.46 0.41 0.41 – 0.29 0.24 0.32 -0.9 -1.24 -1.52 -0.66 -0.58 -0.33 -0.36 -0.16 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 1.7 0 0 0
Bud -1.9 -1.8 -1.5 -2.1 -2.2 – -2.1 -1.2 -1.8 -2.0 -2.2 0 0.7 2.2 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery -1.3 -4.6 -4.4 -5.4 -5.6 – -4.2 -4.6 -2.0 -1.6 -2.0 -3.1 -2.9 -0.5 -0.5
Cytoplasm 3.0 2.1 2.1 4.3 4.1 – 6.3 6.9 5.9 -0.7 -0.7 0.1 4.9 3.2 0
Endoplasmic Reticulum -0.8 -1.2 -4.8 -7.4 -7.3 – -8.7 -9.0 -7.8 -8.1 -10.8 -15.0 -12.2 -10.1 -8.3
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0 0 -2.6 -2.9 -3.4 – -3.2 -2.4 -3.8 9.3 10.6 11.0 2.4 1.2 0
Mitochondria 0 0 0 0 0 – 0 0 0 0 0 0 4.9 6.3 0
Nucleus 0 0 6.3 8.7 8.4 – 5.8 5.3 5.2 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 2.7 1.7 2.9 1.3 1.2 – 0.5 1.6 0.3 3.4 4.1 3.8 6.2 6.3 6.6
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 2.7 3.4 0 0 0
Vacuole 0 0 5.4 5.8 6.2 – 7.8 7.8 6.8 3.1 3.3 6.4 6.6 6.6 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0006 0 0.01 0.012 0 0 0.0072 0 0.0068 0.0012 0.0001 0 – – – – – –
Bud 0.0009 0.0001 0.0001 0.0001 0 0.0001 0.0003 0 0.0001 0.0001 0.0004 0.0001 – – – – – –
Bud Neck 0.0007 0.0001 0.0007 0.0001 0 0.0002 0.0011 0.0001 0.0002 0.0001 0.0001 0.0001 – – – – – –
Bud Periphery 0.0023 0.0003 0.0006 0.0001 0.0002 0.0004 0.0014 0.0004 0.0007 0.0009 0.0017 0.0003 – – – – – –
Bud Site 0.0071 0.0002 0.0003 0.0004 0 0.0001 0.0009 0.0001 0.0003 0.0002 0.0005 0.0001 – – – – – –
Cell Periphery 0.4319 0.4747 0.4766 0.4279 0.4836 0.4614 0.4968 0.5602 0.548 0.5196 0.5532 0.5299 – – – – – –
Cytoplasm 0.0031 0.0008 0.011 0.0023 0.0001 0.0005 0.0024 0.0004 0.0004 0.0002 0.0002 0.0002 – – – – – –
Cytoplasmic Foci 0.0097 0.0042 0.003 0.0219 0 0 0.0193 0 0.0002 0.0014 0.0004 0.0024 – – – – – –
Eisosomes 0.0009 0.0002 0.0003 0.0003 0 0 0.0017 0.0046 0.0005 0.0001 0.0022 0.0012 – – – – – –
Endoplasmic Reticulum 0.0012 0.0007 0.0009 0.0008 0.0009 0.0025 0.0026 0.0017 0.0018 0.0152 0.0019 0.0017 – – – – – –
Endosome 0.0068 0.0002 0.0011 0.0081 0.0001 0.0004 0.0048 0.0002 0.0003 0.0068 0.0001 0.0002 – – – – – –
Golgi 0.0034 0 0.0008 0.003 0 0 0.0031 0 0.0002 0.0277 0 0.0001 – – – – – –
Lipid Particles 0.0346 0.0148 0.0236 0.0168 0.0035 0.0062 0.0201 0.0074 0.0063 0.0042 0.0035 0.0262 – – – – – –
Mitochondria 0.0077 0.0002 0.0026 0.0025 0 0.0002 0.0071 0.0002 0.0017 0.0026 0.0154 0.0078 – – – – – –
None 0.0028 0.0006 0.0007 0.0089 0 0 0.0067 0 0.0007 0 0.0019 0.0008 – – – – – –
Nuclear Periphery 0.0072 0.0012 0.0016 0.0058 0.0006 0.0161 0.0023 0.0015 0.0015 0.0014 0.0007 0.0011 – – – – – –
Nucleolus 0.0274 0.0034 0.0127 0.0073 0.0005 0.0031 0.0069 0.0012 0.0021 0.0013 0.0057 0.0064 – – – – – –
Nucleus 0.0086 0.0011 0.0022 0.0024 0.0002 0.0015 0.0017 0.0003 0.0005 0.0006 0.0008 0.0008 – – – – – –
Peroxisomes 0.0023 0 0.0017 0.0051 0 0 0.0011 0 0.0001 0.0001 0.0004 0.0004 – – – – – –
Punctate Nuclear 0.0015 0.0001 0.0043 0.0146 0 0 0.0016 0 0.0001 0 0 0 – – – – – –
Vacuole 0.4173 0.4857 0.4347 0.4546 0.5062 0.4849 0.3928 0.4167 0.4169 0.4064 0.4065 0.4125 – – – – – –
Vacuole Periphery 0.0221 0.0115 0.0102 0.0051 0.004 0.0223 0.0182 0.0049 0.0106 0.0099 0.0044 0.0077 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 117.165 45.3506 77.7204 130.1673 88.4834 141.8481 128.4184 164.8736 175.6833 137.9708
Translational Efficiency 1.1813 1.2536 1.2527 1.0307 1.1503 1.5527 1.2555 1.0175 1.081 1.0209

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress
Localization
Cell Percentages cytoplasm (38%), ER (10%), mixed (45%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dip5

Dip5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dip5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available