Standard name
Human Ortholog
Description S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 1.0 0.96 0.97 0.96 0.85 0.87 0.77 0.82 0.69 0.79 1.0 0.99 0.99 0.98 0.96 0.99 0.98 0.92 0.95 0.95 0.98 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.13 0 0.25 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.06 0 0.13 0.11 0.09 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 5 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1 0 0 0 1 3 0 6 3 0 0 0 0 0 0 0 1 0 1 0 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 1 1 3 3 8 4 7 11 5 8 0 2 3 8 7 8 0 0 0 0 0 0
Cytoplasm 243 133 127 141 209 222 223 286 178 196 150 176 162 262 279 303 302 219 232 139 129 99 187 234
Endoplasmic Reticulum 0 0 0 2 4 3 11 13 1 2 2 2 1 2 2 13 13 9 1 0 1 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 1 0 1 0 0
Golgi 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 8 13 30 9 54 21 0 0 0 1 0 2 0 2 0 0 0 0
Nucleus 0 0 0 0 0 1 5 3 6 8 5 2 0 0 0 3 0 0 0 1 1 1 0 4
Nuclear Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 0 5 2 9 17 14 30 27 19 24 0 1 1 5 7 1 0 0 1 0 1 1
Unique Cell Count 244 133 127 147 215 232 263 329 231 240 216 223 162 265 281 308 313 222 237 151 137 105 192 249
Labelled Cell Count 245 133 128 150 218 239 274 339 258 253 241 240 163 267 285 333 331 240 237 151 137 105 192 249


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.0 49.2 33.7 28.6 23.6 17.5 14.0 12.7 9.9 9.9 8.3 8.4 34.2 32.9 32.3 42.7 47.0 45.3 39.5 37.2 38.8
Std Deviation (1e-4) 7.8 12.8 8.4 6.0 4.3 4.0 3.3 2.8 2.9 2.6 3.6 2.3 8.0 8.2 7.3 13.4 15.3 17.3 9.1 8.8 8.2
Intensity Change (Log2) – – – -0.23 -0.51 -0.94 -1.27 -1.41 -1.77 -1.77 -2.02 -2.01 0.02 -0.03 -0.06 0.34 0.48 0.43 0.23 0.14 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.3 -1.9 -2.4 -4.6 -4.3 -5.8 -5.1 -6.9 -5.6 0 -1.2 -1.0 -1.4 -2.1 -1.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 4.2 0 6.1 3.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.9 0 4.2 3.9 3.4 3.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 35.637 40.6847 40.0102 36.905 34.5815 37.5958 42.5058 48.5776 45.9209 46.361 42.2346 45.7957 41.8955 48.5491 44.2901 44.9371 45.2412 43.5537
Actin 0.0006 0.0003 0.0001 0.0001 0 0.0006 0.0002 0.0001 0.0002 0 0.0006 0.0001 0.0051 0.0001 0.0004 0.0001 0.0002 0.0004
Bud 0.0004 0.0001 0 0 0 0.001 0.0001 0.0003 0.0001 0 0.0003 0.0001 0.0004 0.0001 0.0003 0 0.0001 0.0008
Bud Neck 0.0004 0.0002 0.0002 0.0002 0.0004 0.0008 0.0001 0.0001 0.0002 0.0003 0.001 0.0005 0.0011 0.0001 0.0002 0.0002 0.0002 0.0006
Bud Periphery 0.0002 0 0 0 0 0.0003 0 0 0 0 0.0002 0 0.0005 0 0 0 0 0.0001
Bud Site 0.0004 0.0001 0 0 0 0.0003 0 0.0001 0.0001 0 0.0001 0 0.0028 0.0001 0.0001 0 0 0.0001
Cell Periphery 0.0001 0.0001 0 0 0 0 0 0.0001 0 0 0.0006 0 0.0004 0.0022 0 0 0 0
Cytoplasm 0.9335 0.987 0.9805 0.983 0.9726 0.9492 0.9778 0.9901 0.982 0.9245 0.9542 0.9671 0.9097 0.984 0.9785 0.9743 0.9704 0.9526
Cytoplasmic Foci 0.0013 0.0003 0.0002 0.0007 0.0006 0.007 0.0005 0.0001 0.0003 0.0001 0.0002 0.0004 0.0009 0.0003 0.0031 0.0002 0.0002 0.0038
Eisosomes 0 0 0 0 0 0 0 0 0 0 0 0 0.0004 0.0001 0 0 0 0
Endoplasmic Reticulum 0.0008 0.0003 0.0004 0.0003 0.0006 0.0007 0.0006 0.0005 0.0004 0.0002 0.0013 0.0006 0.0007 0.0002 0.0002 0.0001 0.0006 0.0007
Endosome 0.0003 0.0001 0.0001 0.0001 0.0004 0.0003 0.0001 0 0.0001 0 0.0001 0.0002 0.0013 0.0001 0.0001 0 0.0001 0.0009
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.0004 0 0 0 0 0.0001
Lipid Particles 0.0002 0 0 0 0 0.0001 0 0 0 0 0.0001 0 0.0008 0.0003 0.0001 0 0 0.0001
Mitochondria 0.0009 0.0001 0 0 0 0.0021 0.0001 0 0 0 0 0 0.0021 0.0001 0.0002 0 0.0001 0.0005
None 0.0019 0.0003 0.0001 0.0003 0.0003 0.0014 0.0004 0.0001 0.0003 0.0001 0.0002 0.0002 0.0016 0.0004 0.0009 0.0002 0.0002 0.0011
Nuclear Periphery 0.0071 0.0017 0.0023 0.0018 0.0038 0.0037 0.0025 0.0013 0.0018 0.0042 0.0067 0.0033 0.0066 0.0014 0.002 0.0026 0.0046 0.0051
Nucleolus 0.0029 0 0 0 0 0.0006 0 0 0 0 0.0001 0 0.0021 0.001 0.0003 0 0 0.0003
Nucleus 0.0456 0.0087 0.0156 0.0117 0.0197 0.0294 0.017 0.0068 0.014 0.0701 0.0312 0.0263 0.0572 0.0078 0.0122 0.0221 0.0231 0.0296
Peroxisomes 0.0001 0 0 0 0 0.0003 0 0 0 0 0 0 0.0005 0 0 0 0 0
Punctate Nuclear 0.0017 0.0003 0.0002 0.0015 0.0002 0.0014 0.0003 0.0001 0.0002 0.0002 0.0003 0.0003 0.0007 0.0001 0.0006 0.0001 0.0001 0.0015
Vacuole 0.0014 0.0003 0.0002 0.0002 0.0011 0.0006 0.0003 0.0002 0.0002 0.0002 0.0028 0.0006 0.0029 0.0015 0.0005 0.0002 0.0002 0.0012
Vacuole Periphery 0.0003 0 0 0.0001 0.0001 0.0001 0 0 0 0 0.0001 0.0001 0.0018 0.0001 0.0002 0.0001 0.0001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 165.285 169.4389 172.88 195.5256 180.655 162.3745 173.104 176.5408 153.7678 199.8603
Translational Efficiency 1.1084 1.1004 1.0895 0.7538 1.098 1.1267 1.0632 0.9181 0.9997 0.9376

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Sam4

Sam4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sam4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available