Standard name
Human Ortholog
Description High-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0.07 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0.35 0.17 0.15 0 0 0 0 0 0
Cytoplasm 0.94 0.92 0.89 0.89 0.84 0.7 0.64 0.54 – 0.45 0.42 0.36 0.98 0.98 0.98 0.27 0.41 0.44 0.71 0.67 0.4 0.71 0.76 0.72
Endoplasmic Reticulum 0 0.06 0 0.05 0.09 0.05 0.05 0 – 0.06 0.07 0.11 0 0 0 0 0 0.05 0.07 0.12 0.24 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0.1 0.24 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.11 0.05 0.39 0.48 0.5 – 0.64 0.61 0.58 0 0 0 0.1 0 0.07 0.11 0.06 0.2 0.11 0.1 0.07
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.09 – 0.07 0.08 0.11 0 0 0 0.11 0.26 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 3 1 0 1 2 2 – 0 2 5 0 0 0 11 5 2 4 5 4 1 2 4
Bud 0 0 2 0 1 3 5 7 – 12 12 17 0 0 0 0 3 0 4 5 6 1 0 2
Bud Neck 2 0 0 0 0 0 0 1 – 0 0 1 2 2 2 0 0 0 3 3 2 0 0 2
Bud Site 0 0 0 0 0 1 0 2 – 2 0 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 4 2 2 2 3 6 – 6 7 16 8 6 5 37 12 6 1 1 3 1 0 1
Cytoplasm 255 205 201 185 215 183 222 242 – 150 120 126 417 351 449 28 29 18 197 157 95 66 115 139
Endoplasmic Reticulum 7 14 10 11 22 13 18 12 – 20 20 39 5 5 7 1 0 2 18 28 56 4 3 6
Endosome 1 0 3 0 1 2 2 7 – 2 0 3 2 0 1 0 1 0 3 1 3 1 1 4
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 7 7 10 0 1 0 0 2 3
Mitochondria 10 9 14 22 14 103 165 225 – 216 174 204 0 1 0 11 2 3 29 13 47 10 15 13
Nucleus 0 0 0 0 0 3 2 4 – 1 5 2 0 0 1 2 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 1 3 4 – 3 2 13 0 0 0 3 1 1 0 0 0 0 0 2
Nucleolus 1 0 2 0 1 1 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 3 – 3 0 6 0 0 0 1 0 0 1 4 4 1 1 3
Vac/Vac Membrane 0 2 5 5 8 11 9 41 – 24 24 40 0 0 1 12 18 3 5 7 5 2 4 3
Unique Cell Count 271 223 225 207 257 261 345 446 336 283 351 425 357 458 105 70 41 278 234 237 94 151 193
Labelled Cell Count 279 231 245 226 264 325 431 556 439 366 477 434 365 467 113 78 45 278 234 237 94 151 193


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.8 4.5 4.0 4.8 3.8 4.1 4.0 – 3.7 4.2 4.8 4.9 5.0 5.2 14.0 14.3 19.3 5.4 5.5 6.2
Std Deviation (1e-4) 1.1 2.4 2.7 1.8 3.9 2.0 3.0 1.8 – 2.0 2.4 2.4 0.8 1.0 1.1 3.3 3.9 4.8 3.4 2.0 3.5
Intensity Change (Log2) – – – -0.18 0.09 -0.24 -0.14 -0.18 – -0.28 -0.12 0.08 0.13 0.15 0.19 1.63 1.66 2.09 0.27 0.28 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 –
Bud Neck – – – – – – – – – – – – 0 0 –
Bud Site – – – – – – – – – – – – 0 0 –
Cell Periphery – – – – – – – – – – – – 0 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 –
Endosome – – – – – – – – – – – – 0 0 –
Golgi – – – – – – – – – – – – 0 0 –
Mitochondria – – – – – – – – – – – – 0 0 –
Nuclear Periphery – – – – – – – – – – – – 0 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 –
Peroxisomes – – – – – – – – – – – – 0 0 –
SpindlePole – – – – – – – – – – – – 0 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 –
Cytoplasm – – – – – – – – – – – – 0 0 –
Nucleus – – – – – – – – – – – – 0 0 –
Vacuole – – – – – – – – – – – – 3.5 6.4 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6369 3.6208 4.17 3.3021 5.329 3.6298 3.1225 4.9294 4.6026 4.0057 3.6538 4.8936 2.0924 6.3688 4.5463 4.4179 2.7705 3.4018
Actin 0.0041 0.0028 0.0143 0.0007 0.0021 0.0029 0.0088 0.0033 0.0043 0.001 0.0136 0.0015 0.0001 0.0015 0.0615 0.001 0.0001 0.0011
Bud 0.0006 0.0109 0.0018 0.0011 0.0006 0.0008 0.0008 0.0067 0.0012 0.0012 0.0013 0.001 0 0.001 0 0 0 0.0001
Bud Neck 0.0003 0.0006 0.0004 0.0009 0.0014 0.0013 0.0003 0.0003 0.0013 0.0001 0.0006 0.0006 0 0.0001 0.0001 0.0001 0.0001 0.001
Bud Periphery 0.0015 0.0135 0.0025 0.0035 0.0058 0.0023 0.003 0.0096 0.0032 0.0033 0.0023 0.0073 0 0.0014 0 0.0001 0 0.0003
Bud Site 0.0003 0.0262 0.0015 0.0007 0.0005 0.0004 0.0017 0.0095 0.0049 0.0002 0.0008 0 0 0.0012 0.0002 0.0001 0 0
Cell Periphery 0.0007 0.0017 0.0009 0.0004 0.0018 0.0007 0.0039 0.0034 0.0023 0.0009 0.0012 0.0015 0.0003 0.0011 0.0004 0.0004 0.0001 0.0002
Cytoplasm 0.2491 0.3589 0.2321 0.3213 0.342 0.3647 0.3012 0.3046 0.2576 0.2355 0.3578 0.286 0.2539 0.266 0.2394 0.3535 0.3411 0.4359
Cytoplasmic Foci 0.0129 0.0212 0.0244 0.0244 0.0054 0.011 0.0143 0.0108 0.0176 0.0071 0.0106 0.0114 0.0006 0.0037 0.0069 0.0016 0.0018 0.0081
Eisosomes 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0.0002 0.0001 0.0003 0.0001 0.0003 0.0001 0 0.0001 0.0001 0 0 0
Endoplasmic Reticulum 0.3985 0.2114 0.3592 0.2356 0.4664 0.2925 0.294 0.2507 0.3333 0.3291 0.2502 0.3306 0.3972 0.383 0.4332 0.2133 0.0036 0.1922
Endosome 0.0393 0.0366 0.0283 0.0316 0.0219 0.0125 0.0132 0.0201 0.0235 0.008 0.0256 0.0239 0.0024 0.0122 0.0141 0.0012 0.0003 0.0053
Golgi 0.0047 0.0022 0.0037 0.0023 0.0017 0.0005 0.0021 0.0064 0.0032 0.0005 0.0107 0.002 0.0001 0.0006 0.0008 0.0003 0 0.0003
Lipid Particles 0.012 0.0167 0.0074 0.0022 0.0071 0.0021 0.0086 0.005 0.0076 0.0016 0.0172 0.006 0.0006 0.0016 0.0042 0.0003 0.0001 0.0007
Mitochondria 0.0023 0.0009 0.0104 0.0045 0.0015 0.0007 0.0017 0.0005 0.0014 0.0008 0.0232 0.0014 0.0001 0.0005 0.0002 0.0001 0 0.0004
None 0.2058 0.2451 0.2676 0.3444 0.1099 0.252 0.3105 0.3375 0.2861 0.3627 0.2165 0.3059 0.3303 0.2348 0.2302 0.4232 0.6511 0.3378
Nuclear Periphery 0.0435 0.0254 0.0285 0.0083 0.0139 0.0301 0.0112 0.0128 0.0189 0.0175 0.0417 0.0116 0.012 0.0722 0.005 0.0025 0.0004 0.0092
Nucleolus 0.0002 0.0007 0.0003 0.0003 0.0001 0.0003 0.0003 0.0007 0.0029 0.0004 0.0004 0.0001 0 0.0003 0 0 0 0
Nucleus 0.0105 0.0094 0.0057 0.0036 0.0061 0.0121 0.0032 0.0054 0.0058 0.0062 0.005 0.0032 0.0005 0.0035 0.0007 0.0012 0.0006 0.0021
Peroxisomes 0.0003 0.0003 0.0009 0.0006 0.0001 0.0002 0.004 0.0001 0.007 0.0002 0.0035 0.0003 0 0.0001 0.0006 0 0 0.0001
Punctate Nuclear 0.0013 0.0044 0.0025 0.0023 0.0008 0.005 0.0058 0.0015 0.0047 0.001 0.0015 0.0005 0 0.001 0.0004 0.0001 0.0002 0.0005
Vacuole 0.0095 0.0095 0.0056 0.0088 0.0094 0.0065 0.01 0.0097 0.011 0.0187 0.0074 0.0041 0.0014 0.0099 0.0018 0.0008 0.0002 0.0031
Vacuole Periphery 0.0023 0.0014 0.0016 0.0022 0.001 0.0013 0.0011 0.0014 0.0019 0.004 0.0085 0.0009 0.0002 0.004 0.0002 0.0001 0 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.6089 36.9052 90.8693 111.9159 51.0933 44.3868 40.5658 52.8503 78.181 45.7594
Translational Efficiency 0.9808 1.1239 0.9164 0.9771 0.9448 1.2 0.908 0.8093 0.777 0.7526

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description High-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p
Localization
Cell Percentages ER (12%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Sam3

Sam3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sam3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available