Standard name | |
---|---|
Human Ortholog | |
Description | Putative zinc finger protein; YPR013C is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 5 | 6 |
Bud | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 8 | 2 | 9 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 4 | 14 | 13 |
Bud Neck | 0 | 2 | 0 | 2 | 4 | 4 | 3 | 7 | 4 | 7 | 2 | 8 | 0 | 2 | 3 | 3 | 0 | 1 | 1 | 1 | 2 | 2 | 7 | 12 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 151 | 132 | 107 | 144 | 191 | 171 | 171 | 213 | 181 | 161 | 124 | 127 | 171 | 285 | 361 | 277 | 228 | 205 | 129 | 111 | 87 | 104 | 192 | 223 |
Endoplasmic Reticulum | 0 | 3 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 31 | 11 | 8 | 2 | 0 | 1 | 2 | 4 | 6 |
Endosome | 0 | 1 | 0 | 0 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 12 | 31 | 8 | 3 | 2 | 3 | 4 | 3 | 1 | 3 | 4 | 7 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 1 | 0 | 0 | 2 | 0 |
Mitochondria | 75 | 7 | 0 | 11 | 11 | 45 | 45 | 75 | 182 | 128 | 197 | 144 | 3 | 2 | 0 | 3 | 5 | 7 | 34 | 14 | 27 | 24 | 38 | 27 |
Nucleus | 4 | 6 | 0 | 2 | 12 | 16 | 16 | 25 | 26 | 21 | 24 | 29 | 13 | 4 | 3 | 1 | 1 | 0 | 10 | 15 | 0 | 20 | 19 | 19 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 1 | 0 | 4 | 6 | 1 | 2 | 6 | 3 | 6 | 2 | 13 | 0 | 0 | 1 | 3 | 0 | 0 | 11 | 9 | 3 | 21 | 44 | 32 |
Vac/Vac Membrane | 17 | 17 | 2 | 3 | 21 | 18 | 14 | 15 | 11 | 7 | 17 | 20 | 31 | 45 | 46 | 15 | 17 | 12 | 47 | 31 | 11 | 37 | 42 | 46 |
Unique Cell Count | 215 | 157 | 108 | 161 | 237 | 230 | 227 | 306 | 336 | 275 | 295 | 285 | 224 | 344 | 406 | 298 | 244 | 225 | 261 | 205 | 144 | 236 | 402 | 422 |
Labelled Cell Count | 248 | 169 | 110 | 169 | 254 | 257 | 252 | 344 | 417 | 333 | 380 | 349 | 230 | 369 | 424 | 343 | 267 | 246 | 261 | 205 | 144 | 236 | 402 | 422 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | 4.1 | 4.7 | 4.3 | 4.7 | 4.0 | 4.0 | 4.2 | 3.3 | 3.4 | 3.2 | 3.5 | 5.1 | 5.1 | 5.2 | 5.8 | 5.8 | 5.5 | 4.8 | 5.4 | 5.5 |
Std Deviation (1e-4) | 0.4 | 0.8 | 0.7 | 1.5 | 1.2 | 1.5 | 1.5 | 1.7 | 1.3 | 0.7 | 1.4 | 0.8 | 1.1 | 1.2 | 1.5 | 1.8 | 2.0 | 2.0 | 1.4 | 2.4 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.01 | -0.25 | -0.25 | -0.18 | -0.53 | -0.47 | -0.56 | -0.42 | 0.12 | 0.12 | 0.13 | 0.3 | 0.3 | 0.21 | 0.03 | 0.2 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 2.0 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.0262 | -0.7588 | -0.9071 | -1.0381 | -1.2271 | -0.7395 | -0.1822 | -0.4822 | -0.5957 | -0.3433 | 0.0618 | -0.5833 | -0.7327 | -0.9726 | -1.0155 | -0.7197 | -0.9443 | -1.1855 |
Actin | 0.0023 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0356 | 0.0005 | 0.0091 | 0.0134 | 0.0404 | 0.0013 | 0.0233 | 0.0002 | 0.0238 | 0.0015 | 0.0044 | 0.0109 |
Bud | 0 | 0.0022 | 0.0002 | 0 | 0 | 0 | 0.002 | 0.0033 | 0.0036 | 0.0065 | 0.0005 | 0.0001 | 0.0032 | 0.0077 | 0.0005 | 0.0001 | 0.0005 | 0.0002 |
Bud Neck | 0.0002 | 0.0028 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0.0113 | 0.0005 | 0.0032 | 0.0011 | 0.0013 | 0.0018 | 0.0019 | 0 | 0.0005 | 0.0002 | 0.0004 | 0.0006 |
Bud Periphery | 0.0001 | 0.0005 | 0.0006 | 0 | 0 | 0 | 0.0057 | 0.0046 | 0.002 | 0.0073 | 0.0005 | 0.0001 | 0.0023 | 0.0007 | 0.0007 | 0.0001 | 0.0005 | 0.0004 |
Bud Site | 0.0003 | 0.0131 | 0.0003 | 0 | 0 | 0 | 0.0236 | 0.0175 | 0.0125 | 0.0185 | 0.002 | 0.0001 | 0.0057 | 0.0007 | 0.0009 | 0.0003 | 0.0005 | 0.0002 |
Cell Periphery | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0013 | 0.0004 | 0.0004 | 0.0004 | 0.0001 | 0 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0296 | 0.0394 | 0.0391 | 0.0414 | 0.0432 | 0.0784 | 0.051 | 0.0727 | 0.0714 | 0.0644 | 0.0729 | 0.1116 | 0.1129 | 0.1078 | 0.0674 | 0.0529 | 0.1986 | 0.1664 |
Cytoplasmic Foci | 0.0127 | 0.0077 | 0.0039 | 0.0035 | 0.0035 | 0.0075 | 0.0406 | 0.0072 | 0.0051 | 0.0287 | 0.0336 | 0.0126 | 0.0269 | 0.0031 | 0.0109 | 0.0323 | 0.0233 | 0.0061 |
Eisosomes | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.001 | 0.0011 | 0.0005 | 0.0003 | 0.0005 | 0.0009 | 0.0043 | 0.0022 | 0.0025 | 0.0012 | 0.003 | 0.0012 | 0.0054 | 0.0011 | 0.0022 | 0.0012 | 0.0047 | 0.003 |
Endosome | 0.0018 | 0.0014 | 0.0007 | 0.0003 | 0.0009 | 0.0026 | 0.018 | 0.0012 | 0.0091 | 0.046 | 0.0601 | 0.0026 | 0.0306 | 0.0011 | 0.0101 | 0.0162 | 0.0244 | 0.0051 |
Golgi | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0.011 | 0.0001 | 0.0015 | 0.0079 | 0.046 | 0.0006 | 0.0045 | 0.0002 | 0.0052 | 0.0031 | 0.0121 | 0.0023 |
Lipid Particles | 0.0013 | 0.0009 | 0.0002 | 0.0003 | 0.0004 | 0.0003 | 0.0116 | 0.0004 | 0.0016 | 0.0235 | 0.0252 | 0.0041 | 0.0106 | 0.0001 | 0.008 | 0.0192 | 0.0212 | 0.0013 |
Mitochondria | 0.0054 | 0.0006 | 0.0005 | 0.0002 | 0.0004 | 0.0003 | 0.0111 | 0.0012 | 0.001 | 0.0059 | 0.0126 | 0.0006 | 0.0266 | 0.0002 | 0.0044 | 0.0007 | 0.0056 | 0.0058 |
None | 0.8931 | 0.8321 | 0.9018 | 0.8231 | 0.8944 | 0.7402 | 0.6658 | 0.7926 | 0.7803 | 0.7282 | 0.6241 | 0.6747 | 0.6952 | 0.8592 | 0.8284 | 0.7066 | 0.5911 | 0.6833 |
Nuclear Periphery | 0.0041 | 0.0032 | 0.002 | 0.0021 | 0.0087 | 0.0069 | 0.0098 | 0.0039 | 0.0039 | 0.0036 | 0.0053 | 0.0058 | 0.0153 | 0.0011 | 0.0052 | 0.0076 | 0.0089 | 0.0061 |
Nucleolus | 0.0015 | 0.0031 | 0.0016 | 0.0009 | 0.0017 | 0.0033 | 0.0038 | 0.0035 | 0.0028 | 0.0016 | 0.001 | 0.0031 | 0.0033 | 0.0002 | 0.001 | 0.0011 | 0.0016 | 0.0012 |
Nucleus | 0.0193 | 0.044 | 0.0345 | 0.117 | 0.0156 | 0.0696 | 0.0343 | 0.0588 | 0.0714 | 0.007 | 0.0299 | 0.1412 | 0.0148 | 0.0136 | 0.0141 | 0.0863 | 0.0475 | 0.0833 |
Peroxisomes | 0.0039 | 0.0004 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0217 | 0.0035 | 0.0006 | 0.02 | 0.0136 | 0.0047 | 0.0028 | 0 | 0.0052 | 0.0093 | 0.0329 | 0.0003 |
Punctate Nuclear | 0.0221 | 0.0456 | 0.0123 | 0.0103 | 0.0295 | 0.0872 | 0.0281 | 0.0246 | 0.0157 | 0.0059 | 0.0255 | 0.0329 | 0.0068 | 0.0019 | 0.0092 | 0.0603 | 0.0179 | 0.0215 |
Vacuole | 0.0004 | 0.0005 | 0.0004 | 0.0001 | 0.0003 | 0.0005 | 0.0066 | 0.0012 | 0.0017 | 0.0064 | 0.0013 | 0.0005 | 0.0045 | 0.0007 | 0.0014 | 0.0006 | 0.0026 | 0.0015 |
Vacuole Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0022 | 0.0002 | 0.0004 | 0.0022 | 0.0009 | 0.0002 | 0.0022 | 0.0001 | 0.0006 | 0.0004 | 0.0011 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.2047 | 3.1018 | 4.6034 | 5.4034 | 11.9967 | 6.9358 | 10.7155 | 8.467 | 7.3662 | 8.9162 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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