Standard name
Human Ortholog
Description Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS; overproduction of a truncation allele suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0.05 0.08 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.05 0.12 0.18 0.26 0.34 0.37 0.35 0.19 – 0.15 0.11 0.18 0.35 0.41 0.18 0.19 0.26 0 0 0 0 0.05 0.17
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.13 0.4 0.47 0.46 0.6 0.62 0.64 0.82 – 0.81 0.82 0.08 0 0.16 0.06 0 0.07 0 0 0.06 0 0.06 0.05
Nucleus 0.88 0.76 0.6 0.55 0.35 0.23 0.18 0.19 0.24 – 0.31 0.34 0.68 0.54 0.37 0.6 0.63 0.48 0.78 0.72 0.54 0.57 0.44 0.2
Nuclear Periphery 0 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09
Vac/Vac Membrane 0 0.1 0 0 0.06 0 0 0 0 – 0 0.05 0.06 0.09 0.12 0.2 0.16 0.2 0.11 0.18 0.25 0.24 0.26 0.32
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 1 0 2 3 5 6 3 – 8 4 0 0 1 0 0 1 1 3 1 3 6 10
Bud Neck 0 0 3 0 0 2 2 4 0 – 0 0 0 0 0 0 1 0 0 0 1 0 2 5
Bud Site 0 1 1 0 0 4 3 14 8 – 12 21 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 0 0 0 1 – 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Cytoplasm 4 15 32 34 54 77 108 133 49 – 37 29 34 109 133 44 49 61 1 12 10 6 16 58
Endoplasmic Reticulum 1 1 1 0 1 0 0 2 0 – 0 0 2 4 2 8 12 10 0 0 3 1 0 5
Endosome 0 7 2 0 3 1 1 0 0 – 0 0 3 4 4 7 9 13 0 3 6 4 10 9
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 2 0 0 0 2 0 0 0 1
Mitochondria 0 41 106 87 95 136 183 243 210 – 198 208 15 14 53 15 5 16 0 4 19 3 16 17
Nucleus 42 237 158 101 73 53 54 70 61 – 75 87 128 171 120 143 159 112 55 266 183 129 133 68
Nuclear Periphery 0 15 0 0 0 0 1 1 1 – 0 1 0 0 0 1 0 0 0 2 0 0 1 0
Nucleolus 0 10 0 1 0 0 0 1 0 – 4 1 1 0 0 0 0 1 1 2 4 9 8 5
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 4 3 2 2 5 6 1 – 5 8 1 1 1 4 4 6 1 4 12 8 16 30
Vac/Vac Membrane 2 32 7 1 12 8 5 7 6 – 7 13 12 28 38 48 40 46 7 65 86 55 80 107
Unique Cell Count 48 312 263 185 207 228 295 377 257 244 253 187 315 328 239 253 231 71 372 341 228 303 334
Labelled Cell Count 49 360 315 227 243 287 367 487 340 346 373 196 331 355 272 280 266 71 372 341 228 303 334


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 4.3 3.7 3.6 3.4 3.2 3.2 3.0 2.6 – 2.9 2.8 4.6 4.6 4.1 6.5 7.0 6.4 5.2 5.0 5.0
Std Deviation (1e-4) 0.8 0.8 0.7 1.0 0.8 0.9 1.5 1.3 0.7 – 0.6 1.3 1.4 1.3 1.3 1.6 2.1 2.0 1.4 1.2 1.2
Intensity Change (Log2) – – – -0.06 -0.11 -0.21 -0.22 -0.29 -0.5 – -0.36 -0.4 0.31 0.32 0.16 0.81 0.91 0.79 0.48 0.43 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.2 5.2 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3345 2.6059 2.5612 2.0019 2.2718 2.5304 -0.2439 -0.29 -0.2093 -0.5978 -0.2427 -0.2502 5.2127 5.3894 4.8152 6.1536 4.1832 5.2864
Actin 0.0232 0.0001 0.0111 0 0.003 0.0012 0.0066 0 0 0 0.0003 0 0.0058 0 0.0005 0.0008 0.0043 0.0006
Bud 0.0004 0.0018 0.0032 0 0.0002 0.0001 0.0003 0 0 0 0 0 0.0002 0 0.0001 0.0002 0.0001 0
Bud Neck 0.0089 0.0003 0.0004 0.0002 0.0011 0.0021 0.001 0.0002 0.0001 0.0001 0.0003 0.0003 0.0007 0.0001 0.0004 0.0027 0.0006 0.0009
Bud Periphery 0.001 0.0003 0.0006 0 0.0007 0.0002 0.0003 0 0 0 0 0 0.0005 0 0.0003 0.0008 0.0001 0.0001
Bud Site 0.002 0.0009 0.0005 0 0.0003 0.0002 0.002 0.0001 0 0 0 0 0.0004 0 0.0011 0.0003 0.0003 0
Cell Periphery 0.0004 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Cytoplasm 0.0094 0.0107 0.0046 0.0004 0.0005 0.0008 0.0012 0.0026 0.0001 0.0003 0.0003 0.0002 0.0009 0.0006 0.0008 0.0091 0.0007 0.0003
Cytoplasmic Foci 0.0097 0.0004 0.0018 0 0.0025 0.0007 0.0018 0 0 0 0.0023 0 0.0003 0.0001 0.0001 0.0002 0.0109 0
Eisosomes 0.0003 0 0 0 0 0 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0053 0.0009 0.0002 0 0.0004 0.0008 0.0003 0.0001 0 0 0.0002 0 0.0024 0.0001 0.0002 0.0011 0.001 0.0002
Endosome 0.0206 0.0012 0.002 0 0.0062 0.0055 0.0012 0 0 0 0.0074 0 0.0027 0.0002 0.0003 0.0016 0.0087 0.0003
Golgi 0.0054 0 0.0022 0 0.0021 0.0023 0.0013 0 0 0 0.001 0 0.0011 0 0 0.0001 0.0024 0.0001
Lipid Particles 0.0126 0.0001 0.0025 0 0.004 0.002 0.0023 0 0 0 0.0167 0 0.0016 0 0.0001 0.0003 0.0072 0
Mitochondria 0.007 0.0004 0.0011 0.0001 0.0038 0.0012 0.0005 0.0001 0 0 0.0006 0 0.0025 0.0001 0.001 0.0016 0.0012 0.0006
None 0.0029 0.0095 0.0005 0 0.0005 0.0004 0.0002 0.0025 0 0 0.0006 0.0001 0.0004 0.0006 0.0006 0.0195 0.0009 0.0004
Nuclear Periphery 0.0452 0.0122 0.0041 0.002 0.0199 0.0121 0.0028 0.0044 0.0008 0.0019 0.0048 0.0009 0.0087 0.0017 0.0085 0.0203 0.0111 0.0015
Nucleolus 0.0183 0.0148 0.0088 0.0171 0.0354 0.0266 0.0073 0.0073 0.0062 0.0052 0.0217 0.0155 0.0086 0.0109 0.0109 0.0093 0.032 0.0174
Nucleus 0.7776 0.9352 0.9483 0.9767 0.8808 0.9372 0.9483 0.9675 0.9801 0.989 0.9291 0.9625 0.9563 0.9824 0.9714 0.9129 0.8793 0.9687
Peroxisomes 0.0151 0 0.0015 0 0.0072 0.0004 0.005 0 0 0 0.0012 0 0.0003 0 0.0001 0.0001 0.0114 0
Punctate Nuclear 0.0282 0.0096 0.006 0.0034 0.0292 0.005 0.0173 0.0151 0.0127 0.0033 0.0129 0.0203 0.0053 0.0029 0.0032 0.0163 0.0266 0.0086
Vacuole 0.0044 0.0011 0.0003 0 0.0011 0.0005 0.0004 0.0001 0 0 0.0002 0 0.0007 0.0001 0.0003 0.0018 0.0005 0.0002
Vacuole Periphery 0.0024 0.0003 0.0001 0 0.0013 0.0004 0.0001 0 0 0 0.0004 0 0.0005 0 0.0003 0.0008 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.9196 13.3372 9.6817 15.5311 16.8208 11.9939 16.7833 15.4332 12.9619 20.0715
Translational Efficiency 0.6029 0.6167 0.8142 0.4074 0.5247 0.807 0.5391 0.5772 0.6287 0.5729

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS; overproduction of a truncation allele suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P
Localization
Cell Percentages nucleus (86%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Sdd4

Sdd4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sdd4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available