Standard name
Human Ortholog
Description Catalytic subunit of i-AAA protease complex; complex is located in mitochondrial inner membrane; responsible for degradation of unfolded or misfolded mitochondrial gene products; serves as nonconventional translocation motor to pull PNPase into intermembrane space; also has role in intermembrane space protein folding; mutation causes elevated rate of mitochondrial turnover; human homolog YME1L1 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.11 0.05 0.08 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0 0.08 0.07 0 0.18 0.14 0.11 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0.11 0.09 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.06 0 0 0 0 0 0 0 0.05 0 0.06 0 0.05 0 0 0 0 0.05 0 0
Golgi 0.05 0 0 0 0 0 0 0 0 0 0 0 0.24 0.42 0.47 0.16 0.24 0.45 0.07 0.06 0.06 0.09 0.13 0.11
Mitochondria 0.91 0.92 0.88 0.91 0.81 0.87 0.87 0.86 0.92 0.93 0.9 0.91 0.66 0.65 0.55 0.13 0 0 0.81 0.74 0.73 0.71 0.6 0.63
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.07 0.05 0.08 0.07 0.09 0.07 0.09 0 0 0 0 0.1 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0 0.06 0.1 0.1 0.12 0.08 0.07 0.11 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0.05 0.06 0 0 0 0 0 0 0 0.05 0 0 0.11 0.14 0.08 0 0 0.1 0.07 0.09 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.13 0 0 0.09 0 0 0 0 0 0 0 0.29 0.24 0.17 0.11 0.16 0.12 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 0 0 1 1 0 1 0 0 0 0 0 7 3 0 1 4 5 10 13 1 7 7
Bud 2 5 5 8 4 5 6 12 12 15 25 12 0 3 0 9 3 7 1 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0
Bud Site 1 1 1 1 0 6 7 7 8 15 7 7 0 0 0 3 2 0 – – – – – –
Cell Periphery 16 14 15 5 5 1 2 1 2 4 1 3 3 8 4 15 8 10 1 1 2 0 0 0
Cytoplasm 1 5 4 12 14 11 10 15 10 7 6 4 4 3 5 9 5 12 0 3 0 0 1 0
Endoplasmic Reticulum 1 2 4 1 0 1 0 1 2 4 2 8 0 3 4 6 1 4 0 1 1 0 5 3
Endosome 2 4 13 3 22 4 4 8 5 3 1 6 2 2 7 2 3 3 6 8 12 6 9 7
Golgi 13 7 5 5 7 1 1 1 0 0 1 0 9 47 57 13 14 41 19 18 20 9 27 20
Mitochondria 239 273 284 251 284 299 456 377 435 485 427 480 25 73 67 11 2 3 225 229 246 81 130 118
Nucleus 0 1 1 0 1 3 4 1 2 4 3 3 0 2 0 3 2 1 0 1 0 0 0 0
Nuclear Periphery 8 9 13 3 11 24 28 37 34 45 31 49 0 1 2 3 6 3 0 1 0 0 3 1
Nucleolus 9 10 15 7 22 33 55 51 36 39 51 43 0 1 1 0 0 1 0 2 0 0 0 0
Peroxisomes 4 5 28 13 20 9 17 18 9 15 12 8 2 3 4 9 8 7 6 6 35 8 20 12
SpindlePole 1 1 2 4 2 4 7 12 6 7 7 11 1 1 3 4 5 3 1 1 0 1 1 0
Vac/Vac Membrane 63 39 14 8 33 15 23 10 8 10 8 11 11 27 21 9 9 11 7 20 2 2 5 8
Unique Cell Count 263 296 324 277 350 342 524 439 475 524 473 525 38 113 122 83 58 91 277 311 338 115 217 187
Labelled Cell Count 361 380 404 321 426 417 620 552 569 653 582 645 57 181 178 96 69 110 277 311 338 115 217 187


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.0 7.8 7.8 8.2 8.2 7.2 6.7 6.6 6.3 5.8 6.0 6.2 9.4 8.9 9.1 6.4 6.6 8.7 8.2 9.6 10.5
Std Deviation (1e-4) 1.2 1.3 1.6 1.5 2.0 1.9 1.7 1.7 1.7 1.6 1.8 1.7 1.6 1.3 1.5 1.3 1.5 1.6 1.8 3.0 2.7
Intensity Change (Log2) – – – 0.08 0.08 -0.12 -0.21 -0.24 -0.32 -0.43 -0.38 -0.32 0.27 0.19 0.23 -0.29 -0.24 0.15 0.08 0.31 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1.1 0 0 0 1.1 0.9 1.2 2.7 0.8 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2.7 0 0 0 0 0 0 0 0
Cell Periphery -1.9 -2.4 -3.7 -4.3 -4.2 -4.0 -3.7 -4.4 -4.0 0 1.0 -0.6 4.2 0 2.3
Cytoplasm 2.4 2.2 1.7 0.7 1.9 0.9 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -2.2 1.3 -2.3 -3.3 -1.8 -2.8 -3.6 -4.0 -2.7 0 0 0.8 0 0 0
Golgi 0 0.4 0 0 0 0 0 0 0 0 11.3 12.3 0 0 11.7
Mitochondria 1.2 -2.3 -0.1 -0.3 -0.7 1.8 2.4 1.2 1.8 -3.6 -5.5 -7.5 -13.5 -13.6 -15.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.2 -0.6 1.7 0.9 2.4 1.9 2.6 1.5 2.9 0 0 0 0 0 0
Nucleolus -1.4 0.9 2.5 3.0 3.4 1.7 1.6 3.1 2.0 0 0 0 0 0 0
Peroxisomes -1.9 -1.5 -3.4 -3.4 -2.6 -4.5 -3.7 -3.9 -5.0 0 -2.1 -2.0 0.6 1.2 -0.3
SpindlePole 0 0 0 0 2.2 0 0 0 0 0 0 0 0 0 0
Vacuole -0.9 2.6 0 0 -1.6 -2.2 -2.1 -2.2 -1.9 0 6.1 4.5 0 0 2.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.6255 10.5204 9.8174 9.1915 8.1964 9.7911 10.4308 11.5011 10.951 10.3939 11.0677 10.9155 12.1084 12.3341 12.1467 11.851 10.804 12.0054
Actin 0.008 0.013 0.0035 0.0021 0.0043 0.0024 0.0169 0.0213 0.005 0.0328 0.0017 0.0037 0.0232 0.0112 0.006 0.0007 0.0009 0.0013
Bud 0.0009 0.0019 0.0004 0.0017 0.0002 0.0001 0.0009 0.002 0.0004 0.0027 0.0054 0.0003 0.0003 0.0009 0.0005 0.0001 0.0001 0.0001
Bud Neck 0.0005 0.0007 0.0009 0.0001 0.0005 0.0001 0.0008 0.0007 0.0002 0.0007 0.0001 0.0001 0.0005 0.0005 0.0002 0 0.0001 0.0004
Bud Periphery 0.002 0.0028 0.0007 0.0069 0.0005 0.0003 0.0015 0.0028 0.001 0.0036 0.0153 0.0007 0.0005 0.0025 0.0011 0.0002 0.0001 0.0002
Bud Site 0.004 0.0133 0.0099 0.0003 0.0005 0.0001 0.002 0.0061 0.0014 0.0122 0.0015 0.0008 0.0011 0.0022 0.0054 0.0001 0.0002 0.0001
Cell Periphery 0.0007 0.0013 0.0004 0.0002 0.0006 0.0001 0.0004 0.0007 0.0003 0.0003 0.0004 0.0002 0.0006 0.0011 0.0007 0.0001 0.0001 0.0004
Cytoplasm 0.0034 0.0005 0.0002 0.0001 0.0017 0.0002 0.0028 0.0004 0.0006 0.0012 0.0002 0 0.0008 0.0001 0.0001 0 0.0001 0.0001
Cytoplasmic Foci 0.009 0.0076 0.0024 0.0034 0.0031 0.0019 0.0151 0.008 0.0122 0.0546 0.0014 0.0016 0.01 0.0027 0.0099 0.0003 0.009 0.001
Eisosomes 0.0003 0.0004 0.0003 0.0001 0.0008 0.0001 0.0003 0.0003 0.0006 0.0003 0.0001 0.0002 0.0004 0.001 0.0003 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0018 0.0003 0.0002 0.0002 0.0016 0.0004 0.0041 0.0002 0.0003 0.0005 0.0001 0.0001 0.0017 0.0002 0.0001 0 0.0001 0.0001
Endosome 0.0277 0.0354 0.0123 0.0099 0.0462 0.013 0.0363 0.0289 0.0106 0.0129 0.0044 0.0046 0.0188 0.0135 0.008 0.0018 0.0166 0.0066
Golgi 0.0249 0.0483 0.0108 0.0113 0.0192 0.0347 0.038 0.0373 0.0121 0.0192 0.0257 0.0132 0.019 0.0269 0.0115 0.0044 0.0207 0.0112
Lipid Particles 0.02 0.0376 0.0107 0.0065 0.007 0.0106 0.0456 0.0162 0.0303 0.0183 0.0022 0.0142 0.0477 0.0061 0.0178 0.0032 0.0364 0.0131
Mitochondria 0.839 0.7723 0.9133 0.9101 0.7877 0.9173 0.7637 0.805 0.8885 0.8045 0.9181 0.9298 0.8085 0.8419 0.8947 0.9823 0.8648 0.9488
None 0.0021 0.0001 0.0003 0.0001 0.0008 0.0001 0.0031 0.0001 0.0005 0.0025 0.0001 0.0001 0.0017 0.0001 0.0001 0 0.0001 0.0001
Nuclear Periphery 0.004 0.0004 0.0002 0.0002 0.0271 0.0013 0.0105 0.0002 0.0016 0.0006 0.0001 0.0006 0.0029 0.0002 0.0001 0 0.0001 0.0025
Nucleolus 0.0027 0.001 0.0005 0.0002 0.0091 0.0001 0.0026 0.001 0.005 0.0023 0.0001 0.0002 0.0055 0.0156 0.0098 0.0001 0.0006 0.0002
Nucleus 0.0015 0.0005 0.0003 0.0002 0.0397 0.0002 0.0016 0.0003 0.0008 0.0008 0.0001 0.0001 0.0009 0.001 0.0022 0 0.0001 0.0002
Peroxisomes 0.01 0.0159 0.0047 0.0055 0.0045 0.0019 0.0226 0.0217 0.0138 0.0226 0.0164 0.0097 0.0338 0.0154 0.0218 0.0045 0.0464 0.0048
Punctate Nuclear 0.0023 0.0001 0.0002 0 0.0023 0 0.0032 0.0001 0.0018 0.0033 0 0 0.002 0 0.0003 0 0.0001 0.0008
Vacuole 0.0131 0.0184 0.01 0.0069 0.0285 0.003 0.0109 0.0187 0.0035 0.0023 0.0044 0.0037 0.0092 0.0274 0.0037 0.0005 0.0012 0.0027
Vacuole Periphery 0.0219 0.0283 0.018 0.0338 0.0141 0.0119 0.0172 0.0279 0.0095 0.0018 0.0022 0.0161 0.011 0.0295 0.0057 0.0017 0.0021 0.0051

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.5636 41.3188 51.2093 58.3124 40.1642 62.9044 73.3326 78.1415 83.614 65.9822
Translational Efficiency 0.758 0.9265 0.7843 0.7696 0.7988 0.7645 0.6683 0.6546 0.6903 0.7088

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Catalytic subunit of i-AAA protease complex; complex is located in mitochondrial inner membrane; responsible for degradation of unfolded or misfolded mitochondrial gene products; serves as nonconventional translocation motor to pull PNPase into intermembrane space; also has role in intermembrane space protein folding; mutation causes elevated rate of mitochondrial turnover; human homolog YME1L1 can complement yeast null mutant
Localization
Cell Percentages mitochondrion (95%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Yme1

Yme1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yme1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available