Standard name | |
---|---|
Human Ortholog | |
Description | Reticulon-interacting protein; ER integral membrane protein involved in the generation of tubular ER morphology; promotes membrane curvature; forms tubules in vitro; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Yip1p to mediate membrane traffic and with Sey1p to maintain ER morphology; facilitates lipid exchange between the ER and mitochondria; forms ER foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 30 | 45 | 120 | 69 | 65 | 92 | 105 | 108 | 93 | 111 | 99 | 109 | 46 | 66 | 40 | 29 | 28 | 20 | 37 | 27 | 85 | 30 | 37 | 38 |
Bud | 10 | 6 | 23 | 11 | 14 | 35 | 38 | 31 | 25 | 41 | 17 | 28 | 7 | 9 | 5 | 16 | 23 | 13 | 1 | 4 | 1 | 0 | 0 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 4 | 2 | 2 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 53 | 56 | 68 | 68 | 56 | 50 | 50 | 67 | 36 | 56 | 54 | 51 | 99 | 126 | 171 | 59 | 28 | 42 | 23 | 35 | 44 | 18 | 20 | 29 |
Cytoplasm | 50 | 21 | 22 | 25 | 39 | 79 | 59 | 68 | 66 | 73 | 70 | 85 | 23 | 45 | 42 | 41 | 40 | 31 | 21 | 12 | 5 | 5 | 8 | 15 |
Endoplasmic Reticulum | 172 | 108 | 197 | 149 | 195 | 231 | 307 | 311 | 177 | 201 | 167 | 183 | 230 | 323 | 356 | 17 | 12 | 16 | 226 | 148 | 224 | 198 | 203 | 277 |
Endosome | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 1 | 2 | 2 |
Golgi | 21 | 5 | 54 | 56 | 10 | 10 | 17 | 41 | 25 | 47 | 22 | 26 | 38 | 43 | 22 | 83 | 65 | 53 | 7 | 1 | 8 | 20 | 17 | 28 |
Mitochondria | 0 | 1 | 1 | 3 | 0 | 10 | 19 | 22 | 35 | 24 | 32 | 62 | 0 | 1 | 0 | 14 | 12 | 4 | 7 | 3 | 6 | 13 | 7 | 5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 3 | 4 | 1 | 0 | 0 | 6 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 1 | 1 | 0 | 0 | 0 | 5 | 5 | 1 | 12 | 1 | 4 | 1 | 2 | 3 | 3 | 2 | 2 | 0 | 4 | 2 | 5 | 2 | 3 |
Unique Cell Count | 277 | 188 | 341 | 251 | 293 | 386 | 453 | 520 | 334 | 411 | 341 | 409 | 333 | 490 | 519 | 221 | 166 | 147 | 331 | 249 | 391 | 301 | 307 | 413 |
Labelled Cell Count | 338 | 244 | 489 | 383 | 380 | 508 | 603 | 659 | 459 | 570 | 467 | 555 | 445 | 615 | 639 | 270 | 218 | 182 | 331 | 249 | 391 | 301 | 307 | 413 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 40.3 | 39.9 | 29.6 | 31.4 | 27.8 | 24.4 | 23.3 | 23.4 | 22.2 | 21.6 | 22.2 | 22.2 | 39.1 | 39.2 | 41.0 | 48.1 | 48.3 | 49.7 | 38.6 | 39.8 | 42.1 |
Std Deviation (1e-4) | 7.7 | 7.7 | 6.2 | 6.6 | 5.4 | 5.2 | 5.3 | 5.5 | 5.5 | 5.3 | 5.7 | 6.3 | 7.0 | 8.1 | 8.6 | 14.8 | 16.6 | 14.1 | 7.4 | 7.6 | 9.9 |
Intensity Change (Log2) | – | – | – | 0.09 | -0.09 | -0.28 | -0.34 | -0.34 | -0.42 | -0.45 | -0.41 | -0.41 | 0.4 | 0.41 | 0.47 | 0.7 | 0.71 | 0.75 | 0.38 | 0.43 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.0 | -3.6 | -3.4 | -3.7 | -4.7 | -2.1 | -2.4 | -1.7 | -2.5 | -6.4 | -7.4 | -10.1 | -5.8 | -4.3 | -4.8 |
Bud | -1.2 | -1.1 | 1.2 | 0.9 | -0.5 | 0.4 | 1.6 | -1.0 | 0.1 | -2.9 | -3.6 | -4.7 | 0.2 | 2.6 | 0.8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 2.0 | -0.3 | -2.5 | -3.5 | -2.8 | -3.3 | -2.3 | -1.4 | -2.8 | 2.9 | 1.9 | 4.2 | 1.9 | -0.8 | 2.1 |
Cytoplasm | 1.6 | 2.9 | 5.5 | 3.0 | 3.1 | 5.1 | 4.6 | 5.4 | 5.6 | 0.2 | 1.4 | 0.9 | 4.4 | 5.7 | 4.8 |
Endoplasmic Reticulum | 0.4 | 2.3 | 0.6 | 2.9 | 0.6 | -1.2 | -2.4 | -2.3 | -3.6 | 3.0 | 2.4 | 3.2 | -11.9 | -10.8 | -9.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 2.0 | -5.2 | -6.3 | -5.9 | -3.6 | -3.4 | -1.8 | -3.9 | -4.2 | -1.7 | -3.1 | -5.9 | 5.9 | 5.8 | 5.0 |
Mitochondria | 0 | 0 | 2.5 | 3.5 | 3.5 | 5.9 | 4.2 | 5.5 | 7.3 | 0 | 0 | 0 | 4.3 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.0703 | 0.0155 | 0.0415 | 0.0067 | 0.0735 | 0.0313 | 0.0422 | 0.0213 | 0.0481 | 0.0149 | 0.0075 | 0.0174 | 0.2311 | 0.06 | 0.0952 | 0.2392 | 0.1638 | 0.1311 |
Bud | 0.0022 | 0.0027 | 0.0081 | 0.0022 | 0.0034 | 0.0007 | 0.0041 | 0.0009 | 0.0039 | 0.0039 | 0.0006 | 0.0012 | 0.0073 | 0.0029 | 0.0073 | 0.0079 | 0.0129 | 0.0009 |
Bud Neck | 0.0089 | 0.0056 | 0.0099 | 0.0111 | 0.0129 | 0.0614 | 0.0045 | 0.0038 | 0.0094 | 0.0079 | 0.0141 | 0.0407 | 0.0125 | 0.0135 | 0.0157 | 0.0494 | 0.1751 | 0.0814 |
Bud Periphery | 0.0081 | 0.0066 | 0.0435 | 0.0205 | 0.0134 | 0.0065 | 0.017 | 0.0047 | 0.0295 | 0.0473 | 0.0086 | 0.0104 | 0.0271 | 0.0093 | 0.0464 | 0.0295 | 0.1469 | 0.0076 |
Bud Site | 0.0114 | 0.0484 | 0.0179 | 0.0099 | 0.0025 | 0.0006 | 0.0055 | 0.0113 | 0.0146 | 0.001 | 0.0005 | 0.0004 | 0.0105 | 0.1262 | 0.0206 | 0.0209 | 0.0062 | 0.0005 |
Cell Periphery | 0.2965 | 0.3023 | 0.2893 | 0.3095 | 0.201 | 0.2001 | 0.2403 | 0.2877 | 0.2959 | 0.246 | 0.203 | 0.167 | 0.1684 | 0.3593 | 0.178 | 0.109 | 0.3582 | 0.1451 |
Cytoplasm | 0.0776 | 0.114 | 0.0776 | 0.0246 | 0.0296 | 0.034 | 0.0192 | 0.0431 | 0.0273 | 0.0079 | 0.0117 | 0.0112 | 0.0446 | 0.0373 | 0.0384 | 0.0119 | 0.0035 | 0.0416 |
Cytoplasmic Foci | 0.0067 | 0.0035 | 0.0058 | 0.0056 | 0.0141 | 0.0025 | 0.0038 | 0.0014 | 0.001 | 0.0005 | 0.0011 | 0.001 | 0.0133 | 0.0012 | 0.0015 | 0.0016 | 0.0002 | 0.0026 |
Eisosomes | 0.039 | 0.0314 | 0.0184 | 0.0179 | 0.0764 | 0.0503 | 0.1055 | 0.0701 | 0.0939 | 0.0928 | 0.1196 | 0.0772 | 0.035 | 0.0238 | 0.059 | 0.193 | 0.0542 | 0.0756 |
Endoplasmic Reticulum | 0.2984 | 0.3214 | 0.3761 | 0.4063 | 0.4149 | 0.4877 | 0.3835 | 0.4611 | 0.3983 | 0.5058 | 0.5033 | 0.5678 | 0.2023 | 0.2552 | 0.4359 | 0.227 | 0.037 | 0.3821 |
Endosome | 0.0086 | 0.0028 | 0.0053 | 0.03 | 0.0206 | 0.0049 | 0.0075 | 0.0025 | 0.0023 | 0.0011 | 0.0062 | 0.0038 | 0.0172 | 0.0018 | 0.005 | 0.0205 | 0.0009 | 0.0064 |
Golgi | 0.032 | 0.0215 | 0.017 | 0.0163 | 0.0307 | 0.0624 | 0.0676 | 0.0216 | 0.029 | 0.0179 | 0.0798 | 0.0728 | 0.0885 | 0.015 | 0.042 | 0.0494 | 0.0052 | 0.082 |
Lipid Particles | 0.0586 | 0.0849 | 0.0386 | 0.0719 | 0.045 | 0.0298 | 0.0214 | 0.0344 | 0.0151 | 0.0278 | 0.0155 | 0.0067 | 0.0568 | 0.0479 | 0.0159 | 0.0055 | 0.003 | 0.0126 |
Mitochondria | 0.0187 | 0.0008 | 0.0022 | 0.0075 | 0.0129 | 0.0008 | 0.0127 | 0.001 | 0.0017 | 0.0009 | 0.0026 | 0.0033 | 0.0071 | 0.0007 | 0.0018 | 0.0057 | 0.001 | 0.002 |
None | 0.0035 | 0.0007 | 0.0011 | 0.0007 | 0.0132 | 0.0005 | 0.0073 | 0.0006 | 0.0008 | 0.0006 | 0.0007 | 0.0006 | 0.0025 | 0.0004 | 0.0006 | 0.0008 | 0.0004 | 0.0007 |
Nuclear Periphery | 0.027 | 0.0159 | 0.0168 | 0.0166 | 0.0107 | 0.0147 | 0.0238 | 0.0204 | 0.0156 | 0.014 | 0.0109 | 0.011 | 0.0309 | 0.0202 | 0.0211 | 0.0173 | 0.016 | 0.0167 |
Nucleolus | 0.0011 | 0.001 | 0.0005 | 0.0012 | 0.004 | 0.0003 | 0.0078 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0008 | 0.0006 | 0.0002 | 0.0004 | 0.0009 | 0.0002 |
Nucleus | 0.0022 | 0.0011 | 0.0014 | 0.0017 | 0.0017 | 0.0008 | 0.0036 | 0.0008 | 0.0007 | 0.0006 | 0.0005 | 0.0006 | 0.0022 | 0.0013 | 0.001 | 0.0011 | 0.0025 | 0.001 |
Peroxisomes | 0.0007 | 0.0002 | 0.0009 | 0.0011 | 0.0034 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0014 | 0.0003 | 0.0005 | 0.0003 | 0.0035 | 0.0003 | 0.0019 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0015 | 0.0003 | 0.0003 | 0.0003 | 0.0004 | 0.0002 |
Vacuole | 0.0245 | 0.0188 | 0.0258 | 0.0349 | 0.0103 | 0.0088 | 0.0159 | 0.0118 | 0.0111 | 0.0074 | 0.0116 | 0.0054 | 0.0349 | 0.0218 | 0.012 | 0.0061 | 0.0099 | 0.0072 |
Vacuole Periphery | 0.0026 | 0.0008 | 0.0018 | 0.0036 | 0.0022 | 0.0014 | 0.0039 | 0.0009 | 0.0013 | 0.0013 | 0.002 | 0.0013 | 0.0048 | 0.0011 | 0.0019 | 0.0034 | 0.0016 | 0.0024 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 291.5995 | 208.901 | 231.8464 | 291.0951 | 274.0031 | 156.3814 | 262.1878 | 271.4819 | 228.7441 | 301.8471 |
Translational Efficiency | 1.5087 | 1.8776 | 1.7376 | 1.5037 | 1.6432 | 2.8243 | 1.6156 | 1.5031 | 1.8224 | 1.5916 |
Dataset | Hit |
---|---|
Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|