Standard name
Human Ortholog
Description Reticulon-interacting protein; ER integral membrane protein involved in the generation of tubular ER morphology; promotes membrane curvature; forms tubules in vitro; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Yip1p to mediate membrane traffic and with Sey1p to maintain ER morphology; facilitates lipid exchange between the ER and mitochondria; forms ER foci upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.11 0.24 0.35 0.27 0.22 0.24 0.23 0.21 0.28 0.27 0.29 0.27 0.14 0.13 0.08 0.13 0.17 0.14 0.11 0.11 0.22 0.1 0.12 0.09
Bud 0 0 0.07 0 0.05 0.09 0.08 0.06 0.07 0.1 0.05 0.07 0 0 0 0.07 0.14 0.09 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.19 0.3 0.2 0.27 0.19 0.13 0.11 0.13 0.11 0.14 0.16 0.12 0.3 0.26 0.33 0.27 0.17 0.29 0.07 0.14 0.11 0.06 0.07 0.07
Cytoplasm 0.18 0.11 0.06 0.1 0.13 0.2 0.13 0.13 0.2 0.18 0.21 0.21 0.07 0.09 0.08 0.19 0.24 0.21 0.06 0.05 0 0 0 0
Endoplasmic Reticulum 0.62 0.57 0.58 0.59 0.67 0.6 0.68 0.6 0.53 0.49 0.49 0.45 0.69 0.66 0.69 0.08 0.07 0.11 0.68 0.6 0.58 0.66 0.66 0.67
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.08 0 0.16 0.22 0 0 0 0.08 0.07 0.11 0.06 0.06 0.11 0.09 0 0.38 0.39 0.36 0 0 0 0.07 0.06 0.07
Mitochondria 0 0 0 0 0 0 0 0 0.1 0.06 0.09 0.15 0 0 0 0.06 0.07 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 30 45 120 69 65 92 105 108 93 111 99 109 46 66 40 29 28 20 37 27 85 30 37 38
Bud 10 6 23 11 14 35 38 31 25 41 17 28 7 9 5 16 23 13 1 4 1 0 0 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 4 2 2 2 3
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 53 56 68 68 56 50 50 67 36 56 54 51 99 126 171 59 28 42 23 35 44 18 20 29
Cytoplasm 50 21 22 25 39 79 59 68 66 73 70 85 23 45 42 41 40 31 21 12 5 5 8 15
Endoplasmic Reticulum 172 108 197 149 195 231 307 311 177 201 167 183 230 323 356 17 12 16 226 148 224 198 203 277
Endosome 0 0 0 1 1 1 1 0 0 3 1 1 0 0 0 1 0 0 0 1 4 1 2 2
Golgi 21 5 54 56 10 10 17 41 25 47 22 26 38 43 22 83 65 53 7 1 8 20 17 28
Mitochondria 0 1 1 3 0 10 19 22 35 24 32 62 0 1 0 14 12 4 7 3 6 13 7 5
Nucleus 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 1 3 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 2 1 0 0 0 3 1 1 3 4 1 0 0 6 7 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 1 0 0 0 5 5 1 12 1 4 1 2 3 3 2 2 0 4 2 5 2 3
Unique Cell Count 277 188 341 251 293 386 453 520 334 411 341 409 333 490 519 221 166 147 331 249 391 301 307 413
Labelled Cell Count 338 244 489 383 380 508 603 659 459 570 467 555 445 615 639 270 218 182 331 249 391 301 307 413


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 40.3 39.9 29.6 31.4 27.8 24.4 23.3 23.4 22.2 21.6 22.2 22.2 39.1 39.2 41.0 48.1 48.3 49.7 38.6 39.8 42.1
Std Deviation (1e-4) 7.7 7.7 6.2 6.6 5.4 5.2 5.3 5.5 5.5 5.3 5.7 6.3 7.0 8.1 8.6 14.8 16.6 14.1 7.4 7.6 9.9
Intensity Change (Log2) 0.09 -0.09 -0.28 -0.34 -0.34 -0.42 -0.45 -0.41 -0.41 0.4 0.41 0.47 0.7 0.71 0.75 0.38 0.43 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.0 -3.6 -3.4 -3.7 -4.7 -2.1 -2.4 -1.7 -2.5 -6.4 -7.4 -10.1 -5.8 -4.3 -4.8
Bud -1.2 -1.1 1.2 0.9 -0.5 0.4 1.6 -1.0 0.1 -2.9 -3.6 -4.7 0.2 2.6 0.8
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.0 -0.3 -2.5 -3.5 -2.8 -3.3 -2.3 -1.4 -2.8 2.9 1.9 4.2 1.9 -0.8 2.1
Cytoplasm 1.6 2.9 5.5 3.0 3.1 5.1 4.6 5.4 5.6 0.2 1.4 0.9 4.4 5.7 4.8
Endoplasmic Reticulum 0.4 2.3 0.6 2.9 0.6 -1.2 -2.4 -2.3 -3.6 3.0 2.4 3.2 -11.9 -10.8 -9.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.0 -5.2 -6.3 -5.9 -3.6 -3.4 -1.8 -3.9 -4.2 -1.7 -3.1 -5.9 5.9 5.8 5.0
Mitochondria 0 0 2.5 3.5 3.5 5.9 4.2 5.5 7.3 0 0 0 4.3 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 2.8 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.0703 0.0155 0.0415 0.0067 0.0735 0.0313 0.0422 0.0213 0.0481 0.0149 0.0075 0.0174 0.2311 0.06 0.0952 0.2392 0.1638 0.1311
Bud 0.0022 0.0027 0.0081 0.0022 0.0034 0.0007 0.0041 0.0009 0.0039 0.0039 0.0006 0.0012 0.0073 0.0029 0.0073 0.0079 0.0129 0.0009
Bud Neck 0.0089 0.0056 0.0099 0.0111 0.0129 0.0614 0.0045 0.0038 0.0094 0.0079 0.0141 0.0407 0.0125 0.0135 0.0157 0.0494 0.1751 0.0814
Bud Periphery 0.0081 0.0066 0.0435 0.0205 0.0134 0.0065 0.017 0.0047 0.0295 0.0473 0.0086 0.0104 0.0271 0.0093 0.0464 0.0295 0.1469 0.0076
Bud Site 0.0114 0.0484 0.0179 0.0099 0.0025 0.0006 0.0055 0.0113 0.0146 0.001 0.0005 0.0004 0.0105 0.1262 0.0206 0.0209 0.0062 0.0005
Cell Periphery 0.2965 0.3023 0.2893 0.3095 0.201 0.2001 0.2403 0.2877 0.2959 0.246 0.203 0.167 0.1684 0.3593 0.178 0.109 0.3582 0.1451
Cytoplasm 0.0776 0.114 0.0776 0.0246 0.0296 0.034 0.0192 0.0431 0.0273 0.0079 0.0117 0.0112 0.0446 0.0373 0.0384 0.0119 0.0035 0.0416
Cytoplasmic Foci 0.0067 0.0035 0.0058 0.0056 0.0141 0.0025 0.0038 0.0014 0.001 0.0005 0.0011 0.001 0.0133 0.0012 0.0015 0.0016 0.0002 0.0026
Eisosomes 0.039 0.0314 0.0184 0.0179 0.0764 0.0503 0.1055 0.0701 0.0939 0.0928 0.1196 0.0772 0.035 0.0238 0.059 0.193 0.0542 0.0756
Endoplasmic Reticulum 0.2984 0.3214 0.3761 0.4063 0.4149 0.4877 0.3835 0.4611 0.3983 0.5058 0.5033 0.5678 0.2023 0.2552 0.4359 0.227 0.037 0.3821
Endosome 0.0086 0.0028 0.0053 0.03 0.0206 0.0049 0.0075 0.0025 0.0023 0.0011 0.0062 0.0038 0.0172 0.0018 0.005 0.0205 0.0009 0.0064
Golgi 0.032 0.0215 0.017 0.0163 0.0307 0.0624 0.0676 0.0216 0.029 0.0179 0.0798 0.0728 0.0885 0.015 0.042 0.0494 0.0052 0.082
Lipid Particles 0.0586 0.0849 0.0386 0.0719 0.045 0.0298 0.0214 0.0344 0.0151 0.0278 0.0155 0.0067 0.0568 0.0479 0.0159 0.0055 0.003 0.0126
Mitochondria 0.0187 0.0008 0.0022 0.0075 0.0129 0.0008 0.0127 0.001 0.0017 0.0009 0.0026 0.0033 0.0071 0.0007 0.0018 0.0057 0.001 0.002
None 0.0035 0.0007 0.0011 0.0007 0.0132 0.0005 0.0073 0.0006 0.0008 0.0006 0.0007 0.0006 0.0025 0.0004 0.0006 0.0008 0.0004 0.0007
Nuclear Periphery 0.027 0.0159 0.0168 0.0166 0.0107 0.0147 0.0238 0.0204 0.0156 0.014 0.0109 0.011 0.0309 0.0202 0.0211 0.0173 0.016 0.0167
Nucleolus 0.0011 0.001 0.0005 0.0012 0.004 0.0003 0.0078 0.0002 0.0003 0.0002 0.0002 0.0001 0.0008 0.0006 0.0002 0.0004 0.0009 0.0002
Nucleus 0.0022 0.0011 0.0014 0.0017 0.0017 0.0008 0.0036 0.0008 0.0007 0.0006 0.0005 0.0006 0.0022 0.0013 0.001 0.0011 0.0025 0.001
Peroxisomes 0.0007 0.0002 0.0009 0.0011 0.0034 0.0001 0.0008 0.0001 0.0001 0 0 0 0.0007 0.0001 0.0002 0.0002 0.0001 0.0001
Punctate Nuclear 0.0014 0.0003 0.0005 0.0003 0.0035 0.0003 0.0019 0.0002 0.0002 0.0001 0.0001 0.0001 0.0015 0.0003 0.0003 0.0003 0.0004 0.0002
Vacuole 0.0245 0.0188 0.0258 0.0349 0.0103 0.0088 0.0159 0.0118 0.0111 0.0074 0.0116 0.0054 0.0349 0.0218 0.012 0.0061 0.0099 0.0072
Vacuole Periphery 0.0026 0.0008 0.0018 0.0036 0.0022 0.0014 0.0039 0.0009 0.0013 0.0013 0.002 0.0013 0.0048 0.0011 0.0019 0.0034 0.0016 0.0024

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 291.5995 208.901 231.8464 291.0951 274.0031 156.3814 262.1878 271.4819 228.7441 301.8471
Translational Efficiency 1.5087 1.8776 1.7376 1.5037 1.6432 2.8243 1.6156 1.5031 1.8224 1.5916

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Reticulon-interacting protein; ER integral membrane protein involved in the generation of tubular ER morphology; promotes membrane curvature; forms tubules in vitro; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Yip1p to mediate membrane traffic and with Sey1p to maintain ER morphology; facilitates lipid exchange between the ER and mitochondria; forms ER foci upon DNA replication stress
Localization
Cell Percentages ER (48%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Yop1

Yop1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yop1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available