Standard name | |
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Human Ortholog | |
Description | Gamma-adaptin; large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 3 | 1 | 0 | 0 | 1 | 0 | 1 | 14 | 33 | 15 | 14 | 23 | 23 |
Bud | 2 | 5 | 4 | 4 | 13 | 11 | 28 | 20 | 21 | – | 27 | 30 | 8 | 5 | 9 | 1 | 0 | 0 | 3 | 6 | 5 | 4 | 10 | 10 |
Bud Neck | 3 | 2 | 19 | 6 | 20 | 5 | 13 | 14 | 8 | – | 3 | 7 | 0 | 2 | 0 | 1 | 1 | 1 | 2 | 10 | 5 | 4 | 10 | 11 |
Bud Site | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 4 | 5 | – | 14 | 12 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 4 | 2 | 1 | 1 | 0 | 1 | 2 | – | 0 | 2 | 1 | 4 | 0 | 2 | 2 | 4 | 1 | 1 | 2 | 0 | 2 | 5 |
Cytoplasm | 191 | 282 | 361 | 101 | 181 | 138 | 182 | 210 | 87 | – | 57 | 65 | 180 | 432 | 457 | 178 | 184 | 172 | 106 | 125 | 141 | 44 | 123 | 129 |
Endoplasmic Reticulum | 6 | 4 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 2 | 5 | 22 | 19 | 8 | 2 | 4 | 5 | 7 | 14 | 14 |
Endosome | 8 | 26 | 1 | 4 | 5 | 21 | 9 | 7 | 2 | – | 0 | 11 | 20 | 132 | 110 | 24 | 13 | 13 | 28 | 65 | 12 | 8 | 18 | 17 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 10 | 1 | 0 | 2 | 1 |
Mitochondria | 30 | 29 | 4 | 15 | 31 | 70 | 150 | 122 | 193 | – | 177 | 192 | 16 | 8 | 13 | 7 | 7 | 3 | 129 | 182 | 154 | 73 | 192 | 137 |
Nucleus | 0 | 1 | 1 | 1 | 3 | 3 | 8 | 5 | 2 | – | 6 | 6 | 0 | 2 | 2 | 0 | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1 | 1 | 0 | 1 | 3 | 5 | 6 | 6 | 3 | – | 5 | 6 | 10 | 106 | 50 | 3 | 4 | 1 | 2 | 2 | 1 | 0 | 1 | 4 |
SpindlePole | 12 | 7 | 21 | 18 | 34 | 34 | 36 | 75 | 32 | – | 34 | 62 | 4 | 21 | 15 | 12 | 12 | 8 | 10 | 14 | 13 | 3 | 13 | 11 |
Vac/Vac Membrane | 1 | 16 | 0 | 1 | 1 | 3 | 5 | 6 | 4 | – | 3 | 8 | 4 | 24 | 33 | 15 | 7 | 8 | 16 | 37 | 12 | 8 | 34 | 21 |
Unique Cell Count | 246 | 360 | 385 | 134 | 258 | 250 | 350 | 389 | 276 | 248 | 305 | 227 | 623 | 615 | 224 | 226 | 202 | 330 | 509 | 392 | 174 | 458 | 399 | |
Labelled Cell Count | 260 | 380 | 416 | 153 | 293 | 291 | 442 | 470 | 360 | 327 | 405 | 247 | 739 | 696 | 267 | 251 | 221 | 330 | 509 | 392 | 174 | 458 | 399 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.7 | 5.0 | 4.6 | 5.2 | 4.2 | 4.2 | 4.4 | 3.6 | – | 3.7 | 4.0 | 4.7 | 4.7 | 4.7 | 6.0 | 6.1 | 5.9 | 4.1 | 4.1 | 4.6 |
Std Deviation (1e-4) | 0.5 | 0.9 | 1.5 | 1.3 | 1.5 | 1.5 | 1.2 | 1.2 | 0.9 | – | 1.5 | 1.0 | 1.4 | 1.1 | 0.9 | 1.2 | 1.5 | 1.2 | 1.3 | 1.5 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.07 | -0.23 | -0.23 | -0.17 | -0.46 | – | -0.43 | -0.29 | -0.07 | -0.09 | -0.08 | 0.28 | 0.3 | 0.26 | -0.27 | -0.26 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 5.1 | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.1012 | 8.7767 | 7.623 | 6.4697 | 6.4409 | 7.7253 | 6.4792 | 8.0103 | 6.912 | 6.5926 | 6.9619 | 7.0077 | 5.8046 | 6.7302 | 6.0645 | 6.2038 | 5.44 | 6.0998 |
Actin | 0.02 | 0.0001 | 0.0086 | 0.007 | 0.0009 | 0.0023 | 0.0366 | 0.0066 | 0.0076 | 0.0416 | 0.0008 | 0.002 | 0.0115 | 0 | 0.0102 | 0.0001 | 0.0084 | 0.0035 |
Bud | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0008 | 0.0001 | 0.0003 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0013 | 0.0006 | 0.0011 | 0.001 | 0.0019 | 0.0029 | 0.0019 | 0.0012 | 0.0016 | 0.0025 | 0.0006 | 0.0017 | 0.0007 | 0.0004 | 0.0005 | 0.0003 | 0.0021 | 0.0017 |
Bud Periphery | 0.0008 | 0.0003 | 0.0007 | 0.0008 | 0.0006 | 0.0007 | 0.0012 | 0.0003 | 0.0012 | 0.0021 | 0.0002 | 0.0011 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0003 | 0.0004 |
Bud Site | 0.0014 | 0.0007 | 0.0017 | 0.0008 | 0.0004 | 0.0003 | 0.0031 | 0.0005 | 0.0074 | 0.0026 | 0.0001 | 0.0004 | 0.0008 | 0.0002 | 0.0004 | 0.0001 | 0.0003 | 0.0002 |
Cell Periphery | 0.0007 | 0.0003 | 0.0005 | 0.0004 | 0.0003 | 0.0004 | 0.0004 | 0.0002 | 0.0003 | 0.0011 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.0034 | 0.0001 | 0.0014 | 0.0093 | 0.0002 | 0.0003 | 0.004 | 0.0002 | 0.0026 | 0.003 | 0.0001 | 0.001 | 0.0014 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0002 |
Cytoplasmic Foci | 0.0021 | 0.0001 | 0.0024 | 0.0051 | 0.0018 | 0.0006 | 0.0209 | 0.0002 | 0.0026 | 0.0086 | 0.0006 | 0.0004 | 0.0059 | 0 | 0.0014 | 0 | 0.0043 | 0.0016 |
Eisosomes | 0.0002 | 0 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0013 | 0.0001 | 0.0012 | 0.0024 | 0.0003 | 0.0006 | 0.0112 | 0.0001 | 0.0031 | 0.0027 | 0.0004 | 0.0067 | 0.002 | 0 | 0.0003 | 0 | 0.0004 | 0.0005 |
Endosome | 0.0035 | 0.0006 | 0.0033 | 0.005 | 0.0037 | 0.003 | 0.0266 | 0.0004 | 0.0061 | 0.0241 | 0.0068 | 0.0031 | 0.0056 | 0.0001 | 0.0019 | 0.0003 | 0.0069 | 0.003 |
Golgi | 0.0013 | 0.0001 | 0.0007 | 0.0019 | 0.0032 | 0.0007 | 0.0091 | 0.0007 | 0.0049 | 0.014 | 0.0052 | 0.0017 | 0.0017 | 0 | 0.0007 | 0 | 0.0038 | 0.0018 |
Lipid Particles | 0.0046 | 0.0008 | 0.0107 | 0.0155 | 0.0255 | 0.0036 | 0.019 | 0.0003 | 0.0038 | 0.0127 | 0.0196 | 0.0051 | 0.0142 | 0.0001 | 0.005 | 0.0002 | 0.0118 | 0.0047 |
Mitochondria | 0.0015 | 0.0005 | 0.0024 | 0.0075 | 0.0229 | 0.0009 | 0.0108 | 0.0007 | 0.006 | 0.0088 | 0.0066 | 0.0028 | 0.0035 | 0.0003 | 0.0006 | 0.0007 | 0.0012 | 0.0013 |
None | 0.0038 | 0.0002 | 0.0147 | 0.0546 | 0.0002 | 0.0004 | 0.0026 | 0 | 0.0005 | 0.0016 | 0.0001 | 0.0001 | 0.0013 | 0 | 0.0004 | 0.0001 | 0.0008 | 0.0002 |
Nuclear Periphery | 0.0538 | 0.0123 | 0.0627 | 0.1458 | 0.0602 | 0.0837 | 0.0424 | 0.0079 | 0.0185 | 0.0366 | 0.0085 | 0.0256 | 0.0462 | 0.0137 | 0.0235 | 0.0135 | 0.0846 | 0.0215 |
Nucleolus | 0.5214 | 0.4587 | 0.597 | 0.3506 | 0.1983 | 0.48 | 0.3153 | 0.3476 | 0.4416 | 0.2622 | 0.235 | 0.375 | 0.3711 | 0.4242 | 0.5135 | 0.2615 | 0.2177 | 0.4687 |
Nucleus | 0.3296 | 0.5194 | 0.2532 | 0.3426 | 0.6514 | 0.3947 | 0.4524 | 0.6235 | 0.4804 | 0.5461 | 0.7106 | 0.5636 | 0.5098 | 0.5556 | 0.4169 | 0.7035 | 0.6221 | 0.48 |
Peroxisomes | 0.0006 | 0.0001 | 0.0014 | 0.035 | 0.0014 | 0.0004 | 0.0074 | 0.0002 | 0.0032 | 0.001 | 0.0003 | 0.0004 | 0.0014 | 0 | 0.006 | 0 | 0.0031 | 0.0003 |
Punctate Nuclear | 0.0413 | 0.0023 | 0.0272 | 0.0075 | 0.0203 | 0.0204 | 0.0206 | 0.0052 | 0.0025 | 0.0061 | 0.001 | 0.0056 | 0.0151 | 0.0036 | 0.0151 | 0.0177 | 0.0292 | 0.0085 |
Vacuole | 0.0035 | 0.0018 | 0.0052 | 0.0033 | 0.0028 | 0.0016 | 0.0071 | 0.0023 | 0.0026 | 0.0168 | 0.0009 | 0.0018 | 0.0031 | 0.0006 | 0.0016 | 0.0006 | 0.0007 | 0.0008 |
Vacuole Periphery | 0.0039 | 0.001 | 0.003 | 0.0035 | 0.0033 | 0.0023 | 0.0064 | 0.0018 | 0.0031 | 0.0045 | 0.0024 | 0.0014 | 0.0035 | 0.0006 | 0.001 | 0.0009 | 0.0016 | 0.001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 26.7131 | 28.419 | 23.7241 | 35.5428 | 28.3375 | 27.3602 | 36.5167 | 37.5755 | 35.9943 | 38.0285 |
Translational Efficiency | 0.8397 | 0.8495 | 0.8447 | 0.5971 | 0.6494 | 0.7853 | 0.7051 | 0.5143 | 0.637 | 0.6017 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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