Standard name
Human Ortholog
Description Gamma-adaptin; large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0.08 0.05 0.06
Bud 0 0 0 0 0.05 0 0.08 0.05 0.08 0.11 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.78 0.94 0.75 0.7 0.55 0.52 0.54 0.32 0.23 0.21 0.79 0.69 0.74 0.79 0.81 0.85 0.32 0.25 0.36 0.25 0.27 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.08 0 0 0 0 0.05 0 0
Endosome 0 0.07 0 0 0 0.08 0 0 0 0 0 0.09 0.21 0.18 0.11 0.06 0.06 0.09 0.13 0 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0.08 0 0.11 0.12 0.28 0.43 0.31 0.7 0.71 0.63 0.07 0 0 0 0 0 0.39 0.36 0.39 0.42 0.42 0.34
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.17 0.08 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0 0.05 0.13 0.13 0.14 0.1 0.19 0.12 0.14 0.2 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0.05 0.07 0 0.05 0.07 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 2 1 0 0 0 2 0 0 0 3 1 0 0 1 0 1 14 33 15 14 23 23
Bud 2 5 4 4 13 11 28 20 21 27 30 8 5 9 1 0 0 3 6 5 4 10 10
Bud Neck 3 2 19 6 20 5 13 14 8 3 7 0 2 0 1 1 1 2 10 5 4 10 11
Bud Site 0 1 0 0 0 0 3 4 5 14 12 1 0 0 0 0 0
Cell Periphery 1 0 4 2 1 1 0 1 2 0 2 1 4 0 2 2 4 1 1 2 0 2 5
Cytoplasm 191 282 361 101 181 138 182 210 87 57 65 180 432 457 178 184 172 106 125 141 44 123 129
Endoplasmic Reticulum 6 4 0 0 1 0 0 0 0 0 1 1 2 5 22 19 8 2 4 5 7 14 14
Endosome 8 26 1 4 5 21 9 7 2 0 11 20 132 110 24 13 13 28 65 12 8 18 17
Golgi 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 10 1 0 2 1
Mitochondria 30 29 4 15 31 70 150 122 193 177 192 16 8 13 7 7 3 129 182 154 73 192 137
Nucleus 0 1 1 1 3 3 8 5 2 6 6 0 2 2 0 1 1 0 0 3 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Nucleolus 0 1 0 0 0 0 0 0 1 1 0 0 1 2 0 0 0 0 0 0 0 0 0
Peroxisomes 1 1 0 1 3 5 6 6 3 5 6 10 106 50 3 4 1 2 2 1 0 1 4
SpindlePole 12 7 21 18 34 34 36 75 32 34 62 4 21 15 12 12 8 10 14 13 3 13 11
Vac/Vac Membrane 1 16 0 1 1 3 5 6 4 3 8 4 24 33 15 7 8 16 37 12 8 34 21
Unique Cell Count 246 360 385 134 258 250 350 389 276 248 305 227 623 615 224 226 202 330 509 392 174 458 399
Labelled Cell Count 260 380 416 153 293 291 442 470 360 327 405 247 739 696 267 251 221 330 509 392 174 458 399


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.7 5.0 4.6 5.2 4.2 4.2 4.4 3.6 3.7 4.0 4.7 4.7 4.7 6.0 6.1 5.9 4.1 4.1 4.6
Std Deviation (1e-4) 0.5 0.9 1.5 1.3 1.5 1.5 1.2 1.2 0.9 1.5 1.0 1.4 1.1 0.9 1.2 1.5 1.2 1.3 1.5 1.9
Intensity Change (Log2) -0.1 0.07 -0.23 -0.23 -0.17 -0.46 -0.43 -0.29 -0.07 -0.09 -0.08 0.28 0.3 0.26 -0.27 -0.26 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cyto
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nuclear Periphery 0
Nuc
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vac
Cortical Patches 0
Cytoplasm 0
Nucleus 0
Vacuole 5.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.1012 8.7767 7.623 6.4697 6.4409 7.7253 6.4792 8.0103 6.912 6.5926 6.9619 7.0077 5.8046 6.7302 6.0645 6.2038 5.44 6.0998
Actin 0.02 0.0001 0.0086 0.007 0.0009 0.0023 0.0366 0.0066 0.0076 0.0416 0.0008 0.002 0.0115 0 0.0102 0.0001 0.0084 0.0035
Bud 0.0002 0.0001 0.0002 0.0002 0.0001 0.0001 0.0007 0.0001 0.0004 0.0008 0.0001 0.0003 0.0002 0 0.0001 0.0001 0.0001 0.0001
Bud Neck 0.0013 0.0006 0.0011 0.001 0.0019 0.0029 0.0019 0.0012 0.0016 0.0025 0.0006 0.0017 0.0007 0.0004 0.0005 0.0003 0.0021 0.0017
Bud Periphery 0.0008 0.0003 0.0007 0.0008 0.0006 0.0007 0.0012 0.0003 0.0012 0.0021 0.0002 0.0011 0.0005 0.0001 0.0002 0.0002 0.0003 0.0004
Bud Site 0.0014 0.0007 0.0017 0.0008 0.0004 0.0003 0.0031 0.0005 0.0074 0.0026 0.0001 0.0004 0.0008 0.0002 0.0004 0.0001 0.0003 0.0002
Cell Periphery 0.0007 0.0003 0.0005 0.0004 0.0003 0.0004 0.0004 0.0002 0.0003 0.0011 0.0001 0.0002 0.0003 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.0034 0.0001 0.0014 0.0093 0.0002 0.0003 0.004 0.0002 0.0026 0.003 0.0001 0.001 0.0014 0 0.0006 0.0001 0.0005 0.0002
Cytoplasmic Foci 0.0021 0.0001 0.0024 0.0051 0.0018 0.0006 0.0209 0.0002 0.0026 0.0086 0.0006 0.0004 0.0059 0 0.0014 0 0.0043 0.0016
Eisosomes 0.0002 0 0.0006 0.0003 0.0001 0.0001 0.0002 0 0.0001 0.0003 0 0 0.0001 0 0 0 0.0001 0.0001
Endoplasmic Reticulum 0.0013 0.0001 0.0012 0.0024 0.0003 0.0006 0.0112 0.0001 0.0031 0.0027 0.0004 0.0067 0.002 0 0.0003 0 0.0004 0.0005
Endosome 0.0035 0.0006 0.0033 0.005 0.0037 0.003 0.0266 0.0004 0.0061 0.0241 0.0068 0.0031 0.0056 0.0001 0.0019 0.0003 0.0069 0.003
Golgi 0.0013 0.0001 0.0007 0.0019 0.0032 0.0007 0.0091 0.0007 0.0049 0.014 0.0052 0.0017 0.0017 0 0.0007 0 0.0038 0.0018
Lipid Particles 0.0046 0.0008 0.0107 0.0155 0.0255 0.0036 0.019 0.0003 0.0038 0.0127 0.0196 0.0051 0.0142 0.0001 0.005 0.0002 0.0118 0.0047
Mitochondria 0.0015 0.0005 0.0024 0.0075 0.0229 0.0009 0.0108 0.0007 0.006 0.0088 0.0066 0.0028 0.0035 0.0003 0.0006 0.0007 0.0012 0.0013
None 0.0038 0.0002 0.0147 0.0546 0.0002 0.0004 0.0026 0 0.0005 0.0016 0.0001 0.0001 0.0013 0 0.0004 0.0001 0.0008 0.0002
Nuclear Periphery 0.0538 0.0123 0.0627 0.1458 0.0602 0.0837 0.0424 0.0079 0.0185 0.0366 0.0085 0.0256 0.0462 0.0137 0.0235 0.0135 0.0846 0.0215
Nucleolus 0.5214 0.4587 0.597 0.3506 0.1983 0.48 0.3153 0.3476 0.4416 0.2622 0.235 0.375 0.3711 0.4242 0.5135 0.2615 0.2177 0.4687
Nucleus 0.3296 0.5194 0.2532 0.3426 0.6514 0.3947 0.4524 0.6235 0.4804 0.5461 0.7106 0.5636 0.5098 0.5556 0.4169 0.7035 0.6221 0.48
Peroxisomes 0.0006 0.0001 0.0014 0.035 0.0014 0.0004 0.0074 0.0002 0.0032 0.001 0.0003 0.0004 0.0014 0 0.006 0 0.0031 0.0003
Punctate Nuclear 0.0413 0.0023 0.0272 0.0075 0.0203 0.0204 0.0206 0.0052 0.0025 0.0061 0.001 0.0056 0.0151 0.0036 0.0151 0.0177 0.0292 0.0085
Vacuole 0.0035 0.0018 0.0052 0.0033 0.0028 0.0016 0.0071 0.0023 0.0026 0.0168 0.0009 0.0018 0.0031 0.0006 0.0016 0.0006 0.0007 0.0008
Vacuole Periphery 0.0039 0.001 0.003 0.0035 0.0033 0.0023 0.0064 0.0018 0.0031 0.0045 0.0024 0.0014 0.0035 0.0006 0.001 0.0009 0.0016 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.7131 28.419 23.7241 35.5428 28.3375 27.3602 36.5167 37.5755 35.9943 38.0285
Translational Efficiency 0.8397 0.8495 0.8447 0.5971 0.6494 0.7853 0.7051 0.5143 0.637 0.6017

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Gamma-adaptin; large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
Localization
Cell Percentages nucleus (36%), nuclear periphery (11%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Apl4

Apl4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Apl4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available