Standard name
Human Ortholog
Description Flavin-linked sulfhydryl oxidase localized to the ER lumen; involved in disulfide bond formation within the endoplasmic reticulum (ER)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.99 0.98 0.99 0.96 0.89 0.85 – 0.84 0.83 0.86 0.84 0.97 1.0 0.99 0.8 0.85 0.68 0.27 0.64 0.42 0.31 0.37 0.41
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0 – 0 0 0.05 0 0 0 0 0.05 0 0 0.08 0 0.07 0.09 0 0.06
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.12 0 0 0 0.05 0.05 0.24 – 0.22 0.23 0.2 0.23 0 0 0 0.27 0.21 0.42 0.55 0.25 0.46 0.44 0.46 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 1 0 0 0 0 0 0 0 0 1 1 0 0 3 3
Bud 0 0 0 2 2 5 4 – 2 2 2 1 2 1 1 0 0 0 0 3 0 4 7 4
Bud Neck 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 2 0 2 – 0 0 1 0 1 2 0 0 0 0 0 0 0 0 0 0
Cytoplasm 162 276 164 293 326 203 426 – 324 336 288 341 212 450 496 123 28 52 49 180 70 59 138 141
Endoplasmic Reticulum 1 0 0 0 0 0 1 – 0 0 0 0 2 0 0 5 1 3 1 1 1 5 9 6
Endosome 2 0 1 0 8 5 16 – 3 0 4 0 2 0 0 3 0 2 8 6 1 14 8 8
Golgi 0 0 0 0 0 0 1 – 0 0 0 0 0 1 1 0 0 0 1 0 0 0 0 2
Mitochondria 5 4 0 1 0 22 7 – 17 11 18 18 2 1 0 7 1 2 15 9 12 16 15 21
Nucleus 0 0 1 0 0 3 1 – 10 14 4 5 0 0 0 2 2 1 0 0 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 2 2 1 0 0 0 1 0 1 0 0 0 0 1 0 2
Nucleolus 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 7 0 1 – 1 2 1 2 0 0 0 0 0 0 1 1 0 0 7 4
Vac/Vac Membrane 21 4 3 3 17 12 120 – 85 92 65 95 1 0 1 42 7 32 100 69 77 83 172 143
Unique Cell Count 177 279 167 297 338 227 500 387 404 333 406 218 452 499 153 33 77 182 281 168 192 374 347
Labelled Cell Count 192 286 169 301 364 250 579 444 460 384 463 222 455 500 182 40 92 182 281 168 192 374 347


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.1 4.9 5.2 5.6 4.2 5.8 – 5.3 5.1 4.9 4.8 4.6 4.5 4.7 5.2 5.2 5.9 4.5 4.4 4.5
Std Deviation (1e-4) 0.9 0.8 0.8 2.1 1.1 1.2 1.6 – 2.7 1.2 0.9 0.9 1.1 1.2 1.8 1.2 1.0 1.8 1.9 1.6 1.5
Intensity Change (Log2) – – – 0.08 0.21 -0.21 0.24 – 0.12 0.05 0.0 -0.03 -0.1 -0.11 -0.06 0.09 0.09 0.27 -0.11 -0.14 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 – 0
Bud Neck – – – – – – – – – – – – 0 – 0
Bud Site – – – – – – – – – – – – 0 – 0
Cell Periphery – – – – – – – – – – – – 0 – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 – 0
Endosome – – – – – – – – – – – – 0 – 0
Golgi – – – – – – – – – – – – 0 – 0
Mitochondria – – – – – – – – – – – – 0 – 0
Nuclear Periphery – – – – – – – – – – – – 0 – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 – 0
Peroxisomes – – – – – – – – – – – – 0 – 0
SpindlePole – – – – – – – – – – – – 0 – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 – 0
Cytoplasm – – – – – – – – – – – – 0 – 0
Nucleus – – – – – – – – – – – – 0 – 0
Vacuole – – – – – – – – – – – – 6.6 – 8.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9178 -0.7526 -0.8415 -0.855 -1.0882 -0.7768 0.6423 0.7858 0.9179 0.8076 1.1264 0.7564 1.9601 3.1608 2.2904 1.8174 2.7583 2.5565
Actin 0.0221 0.0001 0.0007 0 0.007 0.0003 0.0398 0.0057 0.0126 0.0004 0.0119 0.0011 0.0432 0.0002 0.0154 0 0.0559 0.0028
Bud 0.0005 0.0004 0.001 0.0012 0.0003 0.0003 0.0019 0.0036 0.0018 0.0008 0.0007 0.0003 0.0004 0.0004 0.0014 0.0003 0.0004 0.0004
Bud Neck 0.0079 0.0005 0.0014 0.0006 0.0014 0.008 0.002 0.001 0.0019 0.0007 0.0013 0.0011 0.0026 0.0002 0.0027 0.0004 0.0024 0.0019
Bud Periphery 0.001 0.0005 0.0019 0.0032 0.0004 0.0005 0.0045 0.0036 0.0019 0.001 0.0016 0.0004 0.0005 0.0004 0.0015 0.0006 0.0005 0.0005
Bud Site 0.0028 0.0014 0.0032 0.0021 0.0005 0.0003 0.0044 0.0066 0.0054 0.0024 0.0012 0.0003 0.0019 0.0013 0.005 0.0006 0.0019 0.0015
Cell Periphery 0.0008 0.0006 0.0004 0.0005 0.0002 0.0002 0.0008 0.001 0.0005 0.0007 0.0004 0.0003 0.0007 0.0004 0.0005 0.0003 0.0002 0.0003
Cytoplasm 0.1961 0.256 0.1528 0.1482 0.1613 0.2617 0.2079 0.2706 0.2301 0.0936 0.1007 0.2551 0.2168 0.267 0.2428 0.1928 0.29 0.3391
Cytoplasmic Foci 0.0683 0.0481 0.0825 0.1036 0.1295 0.0733 0.0388 0.0341 0.044 0.0308 0.06 0.0415 0.059 0.031 0.0434 0.0593 0.0301 0.0472
Eisosomes 0.001 0.0001 0.0001 0 0.0009 0 0.0002 0.0001 0.0003 0 0.0002 0 0.0003 0.0001 0.0002 0 0.0006 0
Endoplasmic Reticulum 0.0098 0.0044 0.0042 0.0029 0.004 0.0037 0.0147 0.0066 0.0068 0.0057 0.0057 0.006 0.01 0.0087 0.0085 0.003 0.0031 0.004
Endosome 0.1018 0.1175 0.1831 0.3001 0.2771 0.1301 0.1533 0.0915 0.1631 0.1994 0.1643 0.1725 0.1235 0.0995 0.1468 0.2499 0.1069 0.1602
Golgi 0.0056 0.0016 0.0035 0.0027 0.0031 0.0013 0.0074 0.0049 0.0034 0.0026 0.0213 0.0015 0.0062 0.0037 0.005 0.0018 0.0047 0.0034
Lipid Particles 0.0148 0.0067 0.0161 0.0074 0.0073 0.0097 0.0139 0.0056 0.0061 0.0023 0.0348 0.002 0.0133 0.0024 0.0077 0.0025 0.0104 0.0032
Mitochondria 0.008 0.0034 0.0054 0.0078 0.0032 0.0024 0.0054 0.0036 0.0109 0.0011 0.0134 0.0015 0.0023 0.0085 0.0093 0.0021 0.0015 0.0039
None 0.4371 0.3776 0.4001 0.2218 0.2555 0.3624 0.2866 0.3158 0.2887 0.2211 0.2684 0.1774 0.3871 0.4429 0.3435 0.253 0.3744 0.2574
Nuclear Periphery 0.011 0.005 0.0065 0.003 0.008 0.0031 0.022 0.0054 0.0178 0.0033 0.0203 0.0103 0.0109 0.0029 0.0059 0.0049 0.0034 0.0051
Nucleolus 0.0034 0.0022 0.0017 0.0032 0.0026 0.001 0.0013 0.0022 0.0022 0.0007 0.0014 0.001 0.0013 0.0005 0.001 0.0011 0.0008 0.0011
Nucleus 0.008 0.0086 0.0089 0.0054 0.0055 0.0064 0.0104 0.0084 0.0107 0.0034 0.0053 0.0128 0.0059 0.003 0.0042 0.0396 0.0025 0.004
Peroxisomes 0.0029 0.0008 0.0034 0.0015 0.0013 0.0011 0.0021 0.0017 0.0018 0.0003 0.0037 0.0002 0.004 0.0002 0.0031 0.0004 0.0044 0.0009
Punctate Nuclear 0.0098 0.0019 0.0051 0.0014 0.0082 0.0019 0.0039 0.0009 0.0028 0.0005 0.0023 0.0013 0.0061 0.0004 0.0019 0.0007 0.0066 0.0015
Vacuole 0.0801 0.1515 0.1088 0.1613 0.1069 0.1218 0.1641 0.2115 0.1613 0.3858 0.2532 0.2848 0.0963 0.1134 0.1343 0.1625 0.0847 0.147
Vacuole Periphery 0.0071 0.0111 0.0095 0.0218 0.0156 0.0105 0.0144 0.0156 0.0258 0.0434 0.0279 0.0285 0.0079 0.013 0.0158 0.0238 0.0147 0.0145

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 37.3403 50.0699 40.8694 42.1014 45.9926 41.0515 46.4068 47.2669 51.724 46.7513
Translational Efficiency 1.3622 1.1254 1.0649 1.3387 1.0513 1.0414 0.9294 0.9123 0.8606 0.8203

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Flavin-linked sulfhydryl oxidase localized to the ER lumen; involved in disulfide bond formation within the endoplasmic reticulum (ER)
Localization
Cell Percentages cytoplasm (18%), vacuole (9%), mixed (47%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Erv2

Erv2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erv2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available