Standard name
Human Ortholog
Description PUF family mRNA-binding protein; Pumilio homology domain confers RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; binding site composed of two UAAU tetranucleotides, separated by a 3-nt linker; PUF2 has a paralog, JSN1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.06 0 0 0.06 0 0 0 0 0 0 0 0.05 0 0.08 0.09 0.14 0.14 0.17 0 0 0 0 0 0
Cytoplasm 0.97 0.96 0.97 1.0 0.98 0.94 0.91 0.88 0.81 0.81 0.79 0.77 0.99 0.99 1.0 0.88 0.91 0.91 0.96 0.94 0.9 0.97 0.93 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.17 0.19 0.31 0.34 0.36 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.1 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 1 0 1 0
Bud 0 2 8 3 3 5 6 10 11 12 10 13 0 2 9 0 0 0 0 0 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 7 5 4 17 17 12 14 11 11 27 15 23 10 28 37 28 28 33 0 0 0 0 0 0
Cytoplasm 122 222 224 306 509 491 522 637 436 509 296 360 247 359 394 179 182 172 120 218 217 151 267 269
Endoplasmic Reticulum 4 5 1 0 4 4 0 3 7 7 0 0 2 1 0 8 7 5 0 0 2 0 4 8
Endosome 0 0 0 0 0 0 0 2 1 0 0 0 0 1 0 2 0 2 0 3 1 0 2 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 1 0 2 0 0 1 1
Mitochondria 1 5 5 4 14 70 96 136 170 213 136 191 0 1 2 4 0 1 0 1 9 0 2 0
Nucleus 0 1 0 1 0 1 2 4 1 2 2 1 2 1 0 1 3 5 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 1 1 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 1 2 0 0 1 2 6 18 2 4 8 12 0 1 0 21 20 9 0 1 5 0 1 2
Unique Cell Count 126 232 230 307 518 521 573 720 540 631 375 466 250 362 394 204 199 190 125 233 243 157 289 292
Labelled Cell Count 136 243 242 331 549 585 647 823 640 776 470 603 261 395 443 244 241 230 125 233 243 157 289 292


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.8 6.3 4.9 5.1 5.3 4.8 4.5 4.5 4.1 4.2 4.2 3.9 7.7 8.1 7.4 10.8 11.5 11.3 7.8 7.5 8.4
Std Deviation (1e-4) 1.3 0.8 0.9 0.8 0.9 0.9 1.2 0.8 0.9 0.8 1.0 0.9 1.0 1.1 1.0 4.9 1.6 1.8 1.1 1.3 1.2
Intensity Change (Log2) – – – 0.07 0.13 -0.03 -0.11 -0.12 -0.25 -0.23 -0.22 -0.3 0.67 0.74 0.6 1.15 1.24 1.22 0.69 0.63 0.79


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 -0.9 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.2 1.2 0 0 0 0 0 0 0 1.5 3.1 3.7 4.8 4.8 5.6
Cytoplasm 2.3 0.8 -1.9 -3.1 -4.0 -6.0 -6.1 -6.3 -6.8 1.1 1.7 3.2 -3.9 -2.7 -3.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 5.0 4.9 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.666 4.808 4.3359 3.9438 3.5242 4.2496 4.1516 5.2458 4.6242 4.3317 4.4356 4.8509 4.907 5.8413 5.0375 5.7688 4.8214 5.3991
Actin 0.1036 0.0039 0.0107 0.0311 0.0015 0.016 0.0195 0.0022 0.0135 0.0493 0.06 0.0112 0.0381 0.0009 0.0017 0.0498 0.0286 0.0179
Bud 0.0014 0.0008 0.0004 0.0005 0.0002 0.0005 0.0007 0.0006 0.0011 0.0025 0.0021 0.0002 0.0002 0.0009 0.0005 0.0018 0.0004 0.0001
Bud Neck 0.0156 0.0004 0.0006 0.0013 0.0027 0.0062 0.0024 0.0003 0.0015 0.0082 0.001 0.0013 0.0007 0.0013 0.0005 0.0015 0.001 0.0015
Bud Periphery 0.0021 0.0006 0.0005 0.0005 0.0002 0.0014 0.0009 0.0004 0.0011 0.0017 0.0029 0.0002 0.0002 0.0008 0.0007 0.0034 0.0005 0.0001
Bud Site 0.0141 0.0143 0.0048 0.0007 0.0004 0.0016 0.0033 0.0052 0.0101 0.0474 0.0029 0.0007 0.0033 0.0391 0.0016 0.0075 0.0007 0.0001
Cell Periphery 0.0012 0.0014 0.0008 0.0012 0.0001 0.0011 0.0008 0.0004 0.0007 0.0004 0.0003 0.0002 0.0009 0.0012 0.0005 0.0016 0.0002 0.0003
Cytoplasm 0.6141 0.9488 0.8893 0.8756 0.8715 0.9092 0.6493 0.9275 0.8211 0.7881 0.7663 0.8951 0.8517 0.861 0.9064 0.8632 0.8645 0.932
Cytoplasmic Foci 0.029 0.0032 0.0074 0.0128 0.0064 0.0087 0.0818 0.013 0.0175 0.0286 0.0324 0.0163 0.0291 0.0085 0.0086 0.0064 0.0201 0.005
Eisosomes 0.0018 0 0.0002 0.0001 0.0001 0.0002 0.0003 0 0.0002 0.0001 0.0001 0 0.0038 0.0001 0.0002 0.0002 0.0001 0
Endoplasmic Reticulum 0.022 0.0041 0.0053 0.0044 0.0027 0.0048 0.0152 0.0022 0.0069 0.0052 0.0026 0.0021 0.0033 0.0027 0.0041 0.0019 0.0018 0.0032
Endosome 0.0306 0.0015 0.0135 0.0125 0.0034 0.0114 0.0617 0.0029 0.0286 0.0118 0.0263 0.0133 0.0186 0.004 0.0075 0.0082 0.0121 0.0024
Golgi 0.0199 0.0006 0.0065 0.0082 0.0014 0.007 0.0194 0.0014 0.0122 0.0147 0.0262 0.0043 0.0185 0.0021 0.0044 0.0057 0.0265 0.0059
Lipid Particles 0.013 0.0002 0.0044 0.0137 0.0127 0.0041 0.0247 0.0003 0.0057 0.0029 0.01 0.0022 0.0031 0.0003 0.0073 0.0003 0.0041 0.0004
Mitochondria 0.0168 0.0001 0.0166 0.0184 0.0537 0.0054 0.0076 0.0001 0.0067 0.002 0.008 0.0007 0.0042 0.0002 0.0075 0.0153 0.0117 0.0012
None 0.0542 0.0169 0.0226 0.0037 0.0149 0.009 0.0301 0.0413 0.0484 0.0167 0.0118 0.0392 0.0146 0.0736 0.0461 0.0274 0.0168 0.0256
Nuclear Periphery 0.0034 0.0004 0.0054 0.0015 0.0004 0.0047 0.0207 0.0002 0.0049 0.0019 0.0047 0.0011 0.0003 0.0002 0.0002 0.0003 0.0003 0.0001
Nucleolus 0.0093 0 0.0002 0.0001 0.0001 0.0024 0.0013 0 0.0007 0.0003 0.0007 0.0004 0.0001 0.0001 0.0001 0.0001 0 0
Nucleus 0.0196 0.0008 0.0016 0.0006 0.0007 0.0014 0.0162 0.0004 0.0023 0.003 0.0044 0.0008 0.0004 0.0005 0.0003 0.0006 0.0004 0.0002
Peroxisomes 0.0165 0.0002 0.0023 0.0082 0.0252 0.0014 0.0199 0.0007 0.0084 0.0062 0.007 0.0068 0.0068 0.0003 0.0009 0.0013 0.0091 0.0037
Punctate Nuclear 0.0051 0.0003 0.0042 0.0028 0.0006 0.0011 0.0185 0.0003 0.0052 0.007 0.0283 0.0028 0.0008 0.0002 0.0002 0.0001 0.0004 0.0001
Vacuole 0.0049 0.0013 0.0021 0.0013 0.001 0.0018 0.0041 0.0006 0.0025 0.0016 0.0012 0.0009 0.001 0.0019 0.0007 0.0029 0.0005 0.0002
Vacuole Periphery 0.0019 0.0001 0.0006 0.0006 0.0002 0.0006 0.0017 0 0.0006 0.0003 0.0006 0.0002 0.0002 0.0001 0.0001 0.0005 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.9893 13.7506 9.2966 22.7099 9.0854 12.2988 20.3914 23.2508 20.1916 24.5154
Translational Efficiency 0.9006 0.9069 1.4286 0.6156 1.275 1.2841 0.8734 0.7669 0.9971 0.7881

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description PUF family mRNA-binding protein; Pumilio homology domain confers RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; binding site composed of two UAAU tetranucleotides, separated by a 3-nt linker; PUF2 has a paralog, JSN1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Puf2

Puf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Puf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available