Standard name
Human Ortholog
Description Catalytic subunit of the NatC type N-terminal acetyltransferase (NAT); involved in subcellular targeting of select N-terminally acetylated substrates to the Golgi apparatus (Arl3p and Grh1p) and the inner nuclear membrane (Trm1p); required for replication of dsRNA virus; human NatC ortholog, Naa60, functionally complements the null, requiring either auxiliary subunit Mak10p or co-expression of human ortholog, Naa35; Naa60, the human NatF gene, also complements the null allele
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0.05 0 0 0 – 0 0 0 0 0.05 0 0 0.08 0 0.07 0 0 0 0 0 0
Cytoplasm 0.97 1.0 – 0.98 0.96 0.86 0.93 – 0.78 0.8 0.71 0.71 0.99 1.0 0.98 0.96 0.93 0.95 0.95 0.97 0.85 0.95 0.95 0.95
Endoplasmic Reticulum 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – 0 0 0.18 0.05 – 0.27 0.25 0.4 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0.07 0 0.06 0.06 – 0.06 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 – 0 0 0 0 0 0 1 0 1 0 0 0 0 0 2 1
Bud 0 0 – 0 0 3 0 – 0 4 2 2 0 2 1 0 0 0 0 0 0 2 0 0
Bud Neck 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 6 0 – 4 5 0 3 – 5 5 1 1 15 13 17 15 6 11 0 0 0 0 0 0
Cytoplasm 303 98 – 84 131 87 157 – 120 143 72 109 300 410 428 190 136 145 295 98 33 164 321 306
Endoplasmic Reticulum 2 0 – 0 0 0 0 – 1 0 0 1 4 1 4 5 9 8 1 0 0 3 7 4
Endosome 0 0 – 0 0 1 1 – 1 0 1 1 0 0 0 0 1 0 2 0 0 0 0 0
Golgi 2 0 – 0 0 0 0 – 0 0 0 0 0 0 1 1 1 1 3 0 0 0 2 1
Mitochondria 1 1 – 2 3 18 9 – 42 44 40 58 0 0 1 2 1 2 1 0 0 0 0 1
Nucleus 0 0 – 2 1 1 2 – 3 5 3 6 2 1 1 2 5 0 0 0 1 0 0 0
Nuclear Periphery 1 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 – 0 0 0 1 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 – 6 6 6 10 – 10 5 2 2 0 0 2 5 4 7 2 1 0 1 0 2
Unique Cell Count 312 98 86 137 101 168 154 178 101 153 304 412 435 197 147 152 310 102 39 174 339 322
Labelled Cell Count 317 99 98 146 116 184 182 207 121 181 322 427 456 220 164 174 310 102 39 174 339 322


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 6.9 – 8.2 8.3 5.4 7.2 – 6.2 5.8 5.7 5.4 9.2 8.0 9.6 8.9 9.0 9.1 8.3 8.8 9.0
Std Deviation (1e-4) 1.0 0.9 – 1.6 1.5 1.2 2.3 – 2.1 1.2 1.6 1.1 1.2 1.5 1.2 2.1 2.1 2.2 1.0 1.5 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6099 3.3707 3.041 2.8145 2.6542 3.1985 3.9278 4.9163 4.7318 4.4283 4.5641 4.7712 5.5958 7.051 6.0873 5.8612 6.2747 5.9806
Actin 0.0037 0.0002 0.0021 0.0002 0.0003 0.0008 0.0095 0.0003 0.0003 0.0001 0.0001 0.0012 0.0207 0.0031 0.0245 0.0033 0.009 0.0075
Bud 0.0001 0.0002 0.0005 0.0002 0.0002 0.0006 0.0003 0.0001 0.0002 0.0003 0.0002 0.0003 0.0006 0.0003 0.0017 0.008 0.0019 0.0007
Bud Neck 0.0002 0.0002 0.0003 0.0003 0.0006 0.0008 0.0006 0.0001 0.0002 0.0002 0.0009 0.0009 0.0034 0.0002 0.0009 0.0013 0.0008 0.0009
Bud Periphery 0.0001 0.0001 0.0002 0.0001 0.0001 0.0006 0.0003 0.0001 0.0002 0.0001 0 0.0002 0.0007 0.0001 0.0014 0.0043 0.001 0.0006
Bud Site 0.0003 0.0013 0.0117 0.0003 0.0004 0.0001 0.0004 0.0005 0.0003 0.0001 0.0001 0.0001 0.0018 0.0015 0.0012 0.0018 0.0009 0.0002
Cell Periphery 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0002 0.0001 0.0002 0 0 0 0.0002 0.0003 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.886 0.941 0.9246 0.9074 0.9016 0.9501 0.9077 0.9607 0.9493 0.9506 0.9785 0.9717 0.8385 0.9461 0.8879 0.9326 0.8051 0.9181
Cytoplasmic Foci 0.0095 0.0078 0.008 0.0114 0.0179 0.0085 0.0129 0.0076 0.0082 0.0085 0.007 0.007 0.033 0.0076 0.0184 0.0098 0.0229 0.0091
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0002 0 0.0002 0 0.0006 0
Endoplasmic Reticulum 0.0098 0.0029 0.0026 0.0011 0.0022 0.0023 0.0116 0.003 0.003 0.0093 0.0008 0.0027 0.0082 0.0013 0.0057 0.0035 0.0042 0.0033
Endosome 0.0125 0.0093 0.01 0.013 0.0257 0.0117 0.0162 0.0082 0.0122 0.0139 0.006 0.0056 0.0149 0.0052 0.012 0.0061 0.0311 0.0098
Golgi 0.0011 0.0006 0.0007 0.0003 0.0013 0.001 0.0031 0.0004 0.0011 0.0003 0.0002 0.0002 0.0042 0.0013 0.005 0.0009 0.0018 0.0027
Lipid Particles 0.0007 0.0001 0.0001 0.0001 0.0004 0 0.001 0 0.0011 0 0 0 0.0048 0.0001 0.002 0.0003 0.0011 0.0064
Mitochondria 0.0005 0.0002 0.0005 0.0002 0.0005 0.0006 0.001 0.0002 0.0006 0.0003 0.0001 0.0002 0.0012 0.0002 0.0017 0.0003 0.0027 0.0007
None 0.0695 0.0316 0.0345 0.0602 0.0424 0.0197 0.0275 0.0144 0.0171 0.0097 0.0026 0.0076 0.0481 0.0307 0.0266 0.0202 0.0623 0.0292
Nuclear Periphery 0.0016 0.0007 0.0005 0.0003 0.0011 0.0003 0.0018 0.0009 0.0009 0.0007 0.0002 0.0003 0.0018 0.0002 0.0012 0.0011 0.0215 0.0011
Nucleolus 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0 0.0002 0 0.0003 0.0001 0.0012 0.0001
Nucleus 0.001 0.0009 0.0007 0.001 0.0016 0.0007 0.0012 0.0011 0.0014 0.001 0.0007 0.0008 0.001 0.0004 0.001 0.002 0.0147 0.0008
Peroxisomes 0.0002 0 0.0001 0.0001 0.0003 0 0.0006 0.0001 0.0006 0 0 0 0.011 0.0003 0.0037 0.0003 0.0019 0.0064
Punctate Nuclear 0.0007 0.0001 0.0002 0.0004 0.0013 0.0002 0.0005 0.0001 0.0002 0.0001 0.0001 0.0002 0.0034 0.0001 0.0021 0.0011 0.0115 0.0009
Vacuole 0.002 0.0022 0.0023 0.0028 0.0017 0.0014 0.0029 0.0018 0.0022 0.004 0.002 0.0008 0.0016 0.0008 0.0018 0.0025 0.0024 0.001
Vacuole Periphery 0.0003 0.0003 0.0004 0.0005 0.0004 0.0004 0.0005 0.0003 0.0005 0.0006 0.0004 0.0002 0.0004 0.0002 0.0004 0.0004 0.0012 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 45.7154 18.5758 24.8113 41.1646 29.636 22.8451 38.5031 34.2268 34.0779 38.2245
Translational Efficiency 1.138 2.1843 1.3772 0.9293 1.4558 2.1284 1.0128 1.0852 1.2475 1.2437

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mak3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available