Standard name
Human Ortholog
Description High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.13 0 0 0 0 0 – 0 0 0 0 0 0.06 0.1 0.13 0 0.09 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.12 – 0.15 0.12 0.13 0.17 0.15 0 0 0 0.05 0.1 0.12 0 0 0 0 0 0
Nucleus 0.86 0.93 0.88 0.87 0.71 0.67 – 0.57 0.66 0.58 0.6 0.58 0.85 0.75 0.67 0.79 0.73 0.71 0.89 0.87 0.83 0.54 0.37 0.3
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0.11 0.19 0.23 0.37 0.51 – 0.63 0.57 0.65 0.65 0.6 0.12 0.27 0.26 0.24 0.26 0.29 0 0.07 0.12 0.21 0.28 0.29
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.21 0.29 0.33
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 – 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 1 0 – 0 0 1 0 1 0 2 1 0 0 0 8 0 0 1 3 6
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 1 0 1 – 3 1 5 3 12 0 1 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 82 5 11 5 11 12 – 4 10 10 7 11 24 49 63 5 15 9 1 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 2 1 1 0 0 0 0
Endosome 1 0 0 0 0 0 – 0 0 0 0 0 1 5 5 0 1 0 1 2 0 2 0 4
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2
Mitochondria 9 4 10 12 17 44 – 64 41 58 62 50 0 4 2 9 18 17 1 0 0 1 6 9
Nucleus 539 293 261 255 230 249 – 242 219 262 224 192 320 368 317 142 128 102 573 291 254 178 119 133
Nuclear Periphery 0 0 1 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 8
Nucleolus 50 35 57 69 120 191 – 268 187 293 243 198 47 134 121 43 45 42 26 24 35 68 92 131
Peroxisomes 1 0 0 0 0 0 – 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 – 0 0 0 0 0 0 4 7 0 0 0 3 0 2 0 4 2
Vac/Vac Membrane 5 2 3 0 5 0 – 3 3 2 1 2 7 8 13 6 2 0 15 9 9 67 93 147
Unique Cell Count 625 316 295 294 322 373 423 330 450 374 332 376 492 471 179 175 144 641 336 306 329 324 448
Labelled Cell Count 689 341 344 342 384 497 584 461 631 541 467 401 578 531 206 211 172 641 336 306 329 324 448


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 40.4 43.5 21.7 30.9 30.7 29.1 – 26.2 27.1 25.6 25.3 25.2 39.2 38.8 37.3 55.0 53.3 48.9 37.6 40.8 38.3
Std Deviation (1e-4) 18.4 13.2 7.9 12.3 11.2 12.4 – 9.5 12.4 11.1 10.5 13.4 14.0 15.7 15.5 21.5 23.0 23.6 12.7 13.8 11.9
Intensity Change (Log2) – – – 0.51 0.5 0.42 – 0.27 0.32 0.23 0.22 0.21 0.85 0.84 0.78 1.34 1.29 1.17 0.79 0.91 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 2.9 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.5 -0.2 -0.4 – -2.6 -0.5 -1.2 -1.5 -0.3 1.5 3.2 4.4 -0.5 2.2 1.2
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 1.1 4.0 – 5.1 4.1 4.4 5.5 5.0 0 -2.6 0 0.9 3.1 3.4
Nucleus -0.6 -5.2 -6.6 – -9.0 -6.5 -8.8 -8.2 -8.6 -1.3 -4.6 -6.6 -2.7 -4.3 -4.6
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.2 4.9 8.5 – 11.7 9.6 12.2 11.8 10.3 -2.4 2.5 2.0 1.2 1.6 2.3
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 1.6 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 47.4194 37.7149 38.2691 38.0886 36.8427 36.2152 37.5153 34.1859 32.314 31.7571 29.6904 31.7681 48.8346 42.5478 40.3615 42.634 39.7615 40.123
Actin 0.0002 0 0 0 0 0 0.0239 0 0.0081 0 0.0003 0.0007 0.0031 0 0.0032 0 0.0001 0.0053
Bud 0 0 0 0 0 0 0.0005 0 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001
Bud Neck 0 0 0 0 0.0001 0.0001 0.0004 0 0.0002 0 0.0002 0.0002 0 0 0.0001 0 0.0001 0.0001
Bud Periphery 0 0 0 0.0001 0.0001 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0001 0.0001
Bud Site 0 0 0 0 0 0 0.0007 0 0.007 0 0.0002 0 0.0001 0 0.0003 0 0.0001 0
Cell Periphery 0.0001 0 0 0.0001 0.0002 0.0002 0.0004 0 0.0002 0.0001 0.0005 0.0001 0.0003 0 0.0002 0 0.0004 0.0002
Cytoplasm 0.0006 0 0 0 0 0 0.0028 0 0.0007 0 0.0009 0.0001 0.0002 0 0.0016 0 0.001 0
Cytoplasmic Foci 0.0003 0 0 0 0.0001 0 0.004 0 0.0057 0 0.0255 0.0005 0.0035 0 0.002 0 0.0178 0.0001
Eisosomes 0 0 0 0 0 0 0.0002 0 0.0001 0 0.0009 0 0.0002 0 0 0 0.0004 0.0001
Endoplasmic Reticulum 0.0002 0 0 0 0 0 0.0051 0 0.0001 0 0.0001 0.0001 0.0003 0 0.0011 0 0.0001 0.0003
Endosome 0.002 0 0 0 0 0 0.0113 0 0.0009 0 0.0001 0.0021 0.0013 0 0.0049 0 0.0001 0.0008
Golgi 0.0001 0 0 0 0 0 0.0026 0 0.0016 0 0.0001 0.0004 0.0007 0 0.0013 0 0.0001 0.0005
Lipid Particles 0.0003 0 0 0 0 0 0.0038 0 0.0022 0 0.0101 0.001 0.0022 0 0.0011 0 0.0092 0.0002
Mitochondria 0.001 0.0003 0.0004 0.0007 0.0019 0.0003 0.0022 0.0003 0.0066 0.0014 0.0165 0.001 0.0033 0.0004 0.0032 0.0003 0.0098 0.0004
None 0.0007 0 0 0 0.0001 0 0.004 0 0.0006 0 0.0049 0.0003 0.0009 0 0.0008 0 0.0048 0.0002
Nuclear Periphery 0.0077 0.0003 0.0005 0.0005 0.0001 0.0001 0.011 0.0006 0.0007 0.0002 0.0007 0.0009 0.003 0.001 0.0014 0.0003 0.0009 0.0008
Nucleolus 0.046 0.0264 0.0378 0.0581 0.4625 0.1739 0.0542 0.0208 0.0353 0.1135 0.2813 0.1822 0.0867 0.0363 0.0384 0.0334 0.4182 0.1581
Nucleus 0.9318 0.9728 0.9598 0.9396 0.5341 0.8245 0.8636 0.978 0.9269 0.8837 0.6509 0.8085 0.8915 0.9618 0.9373 0.9659 0.5317 0.8319
Peroxisomes 0.0001 0 0 0 0 0 0.0037 0 0.0013 0 0.0021 0.0008 0.0004 0 0.0004 0 0.0004 0.0001
Punctate Nuclear 0.0024 0 0.0013 0 0.0001 0 0.0032 0 0.0005 0 0.0005 0.0006 0.0005 0.0001 0.0011 0 0.0014 0.0001
Vacuole 0.0007 0 0 0.0002 0.0003 0.0004 0.0011 0 0.0004 0.0003 0.0013 0.0002 0.0005 0 0.0006 0 0.0008 0.0003
Vacuole Periphery 0.0058 0.0001 0.0001 0.0007 0.0006 0.0003 0.0011 0.0001 0.0005 0.0006 0.0026 0.0002 0.0012 0.0002 0.0007 0.0001 0.0025 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 324.7609 388.2545 316.6522 256.6161 268.8739 269.8325 367.8074 335.3697 290.3135 258.5726
Translational Efficiency 1.779 2.0152 1.9155 2.785 2.3723 2.3099 1.8646 2.5305 2.1241 2.1577

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Nhp6a

Nhp6a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nhp6a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available