Standard name
Human Ortholog
Description snRNP protein component of spliceosomal snRNPs; required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.06 0.08 0.07 0.07 0.11 0.07 0.07 0.1 0.11 0.13 0.23 0.2 0.1 0.16 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.05 0.13 0.61 0.56 0.52 0.57 0.63 0.72 0.57 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.93 0.9 0.89 0.81 0.67 0.66 0.59 0.53 0.53 0.49 0.43 0.85 0.73 0.74 0.8 0.74 0.8 0.92 0.88 0.85 0.66 0.53 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.07 0.09 0.07 0.16 0.12 0.1 0.17 0.06 0.07 0 0.12 0.15 0.12 0 0.07 0.09 0.24 0.29 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 2 1 10 5 6 9 4 2 0 0 0 0 0 0 2 7 2 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 0 2 1 5 13 9 6 8 11 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10 23 20 19 27 39 42 68 37 40 48 54 16 26 28 12 19 9 1 7 3 1 2 2
Endoplasmic Reticulum 0 2 3 0 0 0 0 0 0 0 0 0 1 0 0 5 1 4 0 1 0 0 1 0
Endosome 0 1 4 2 5 0 1 5 1 2 1 7 0 1 4 0 1 0 0 2 2 0 3 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 1 0
Mitochondria 7 25 35 16 46 339 358 327 287 368 357 274 3 0 0 4 3 1 2 4 4 4 5 5
Nucleus 210 567 388 271 280 369 424 368 266 312 242 208 106 83 102 100 87 78 200 552 368 91 100 98
Nuclear Periphery 0 0 4 4 2 1 3 6 0 3 2 2 0 0 1 0 0 0 0 0 1 0 2 3
Nucleolus 0 0 0 1 1 4 5 5 3 1 5 1 0 1 0 0 0 0 0 0 2 2 5 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 2 0 5 0 2 0 1 1 0 0 1 1 5 0 0 1 5
Vac/Vac Membrane 1 27 17 11 18 38 57 44 81 73 50 81 7 8 6 15 17 12 8 41 38 33 55 72
Unique Cell Count 220 610 429 305 345 552 641 625 501 585 493 481 124 114 137 125 117 97 218 630 433 139 191 204
Labelled Cell Count 228 647 471 326 384 796 913 839 687 822 720 635 133 120 143 136 129 105 218 630 433 139 191 204


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.4 5.1 5.1 4.7 3.8 3.8 4.3 3.9 3.6 3.5 3.7 5.7 5.9 6.0 7.1 7.0 7.1 5.2 5.9 5.7
Std Deviation (1e-4) 0.7 0.9 1.4 1.7 1.5 1.8 1.3 1.5 1.3 1.0 1.1 0.8 1.0 1.0 1.0 1.3 1.5 1.2 0.8 1.2 1.0
Intensity Change (Log2) – – – 0.02 -0.11 -0.41 -0.41 -0.25 -0.37 -0.47 -0.53 -0.47 0.17 0.22 0.24 0.48 0.46 0.48 0.03 0.21 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 1.8 1.6 1.3 3.6 1.7 1.5 2.9 3.6 3.3 6.2 5.8 2.1 4.3 1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.5 0 0 0 0 0 0 0 0 -2.2 -3.2 -3.5 -1.9 -2.1 -2.5
Nucleus -0.7 -3.7 -8.7 -9.1 -11.2 -12.4 -12.6 -13.5 -15.0 -1.6 -4.9 -4.8 -3.2 -4.6 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 0.8 2.0 3.1 2.1 6.0 4.7 3.6 6.3 0.8 1.4 0.2 3.4 4.2 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.2914 1.2531 1.1213 1.1006 0.7518 1.053 2.5118 2.236 2.3308 1.9981 1.8335 2.1939 0.8333 1.5049 1.1247 1.4833 0.7042 1.1052
Actin 0.0216 0.001 0.0098 0.0355 0.0058 0.001 0.0384 0.0001 0.0109 0.0299 0.0223 0.0018 0.0476 0.0006 0.023 0 0.0027 0.0003
Bud 0.0007 0.0002 0.0003 0.0005 0.0008 0.0001 0.001 0.0006 0.0007 0.0005 0.0004 0.0001 0.0013 0.0003 0.0001 0 0.0043 0.0001
Bud Neck 0.0026 0.0005 0.0008 0.0003 0.0006 0.0011 0.0059 0.0002 0.0015 0.0039 0.0005 0.0011 0.0012 0.0013 0.0001 0.0001 0.0042 0.0005
Bud Periphery 0.0017 0.0002 0.0005 0.0005 0.0017 0.0001 0.0022 0.0008 0.0022 0.0012 0.0012 0.0001 0.003 0.001 0.0001 0 0.0067 0.0001
Bud Site 0.0099 0.0099 0.0014 0.0007 0.0009 0.0001 0.0029 0.001 0.0035 0.0018 0.0011 0.0003 0.0074 0.0014 0.0005 0 0.054 0.0001
Cell Periphery 0.0005 0.0003 0.0001 0 0.0001 0 0.0006 0 0.0002 0.0003 0.0001 0 0.0003 0.0003 0 0 0.001 0
Cytoplasm 0.0066 0.0127 0.0041 0.0251 0.0017 0.0004 0.0108 0.0092 0.0017 0.0008 0.0004 0.0007 0.0097 0.0019 0.0006 0.0011 0.0015 0.0012
Cytoplasmic Foci 0.011 0.0003 0.0052 0.0026 0.0005 0.0003 0.0236 0.0002 0.007 0.0035 0.0088 0.0009 0.0167 0.0001 0.0039 0 0.0215 0.0008
Eisosomes 0.0002 0 0.0001 0.0001 0.0001 0 0.0003 0 0.0001 0.0007 0.0002 0 0.0003 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0035 0.0006 0.0016 0.0002 0.0011 0.0005 0.0099 0.001 0.0014 0.0015 0.0007 0.0004 0.0018 0.0006 0.0001 0 0.0007 0.0004
Endosome 0.0134 0.0005 0.0094 0.0015 0.0049 0.0039 0.0235 0.0007 0.0112 0.0115 0.0108 0.0028 0.015 0.0009 0.001 0 0.0343 0.0098
Golgi 0.0084 0.0002 0.0016 0.0029 0.0011 0.0007 0.0075 0.0001 0.0053 0.0074 0.0138 0.0011 0.0113 0.0001 0.0023 0 0.0105 0.0006
Lipid Particles 0.012 0.0001 0.0039 0.0025 0.0009 0.0006 0.0174 0.0001 0.0123 0.0035 0.0268 0.0008 0.0176 0.0002 0.0035 0 0.0243 0.0006
Mitochondria 0.0083 0.0003 0.0012 0.0213 0.0067 0.0005 0.0171 0.0104 0.0147 0.0093 0.0157 0.0003 0.0128 0.0034 0.0013 0.0002 0.0176 0.001
None 0.0198 0.0026 0.0013 0.0083 0.0007 0.0002 0.0048 0.0015 0.0056 0.0006 0.0005 0.0001 0.0044 0.0002 0.0002 0.0002 0.001 0.0003
Nuclear Periphery 0.0164 0.0113 0.0149 0.0028 0.0259 0.0071 0.032 0.0205 0.0083 0.015 0.01 0.0014 0.0129 0.0062 0.0019 0.0021 0.0029 0.01
Nucleolus 0.0085 0.008 0.0064 0.0044 0.0093 0.0129 0.0115 0.0086 0.0062 0.0074 0.0109 0.0102 0.0045 0.0076 0.0048 0.0068 0.0187 0.0128
Nucleus 0.832 0.9471 0.9203 0.8767 0.9202 0.9673 0.7515 0.9323 0.8853 0.8919 0.8381 0.9706 0.7935 0.97 0.9489 0.9887 0.755 0.9563
Peroxisomes 0.0027 0 0.0049 0.0121 0.0003 0.0001 0.0078 0.0001 0.0079 0.0039 0.0327 0.0004 0.0199 0.0001 0.0069 0 0.0155 0.0001
Punctate Nuclear 0.0126 0.0034 0.0106 0.0016 0.0126 0.0026 0.0204 0.0115 0.0087 0.002 0.0019 0.0062 0.007 0.0013 0.0004 0.0005 0.0053 0.0032
Vacuole 0.0045 0.0005 0.0011 0.0002 0.0017 0.0003 0.0068 0.0009 0.0033 0.0017 0.0011 0.0004 0.0048 0.002 0.0002 0.0001 0.0157 0.0009
Vacuole Periphery 0.0031 0.0003 0.0006 0.0001 0.0024 0.0002 0.0042 0.0004 0.002 0.0017 0.002 0.0001 0.0069 0.0006 0.0001 0 0.0026 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9808 19.2276 13.5543 14.7827 20.9154 15.0479 17.4969 17.0578 18.493 18.3535
Translational Efficiency 0.7584 0.5982 0.7063 0.762 0.4821 0.7656 0.6899 0.751 0.7211 0.7238

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description snRNP protein component of spliceosomal snRNPs; required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Brr1

Brr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Brr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available