Standard name | |
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Human Ortholog | |
Description | Spermidine synthase; involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 1 | 2 | 5 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 1 | 3 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | 0 | 6 | 1 | 3 | 9 | 7 | 9 | 4 | 11 | 8 | 14 | 0 | 0 | 1 | – | – | – | 0 | 0 | 0 | 0 | 1 | 1 |
Cytoplasm | – | 156 | 170 | 115 | 250 | 221 | 211 | 248 | 205 | 207 | 186 | 225 | 96 | 125 | 156 | – | – | – | 48 | 150 | 152 | 51 | 178 | 200 |
Endoplasmic Reticulum | – | 0 | 2 | 4 | 2 | 7 | 8 | 18 | 10 | 7 | 14 | 17 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 2 | 0 | 2 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 2 | 1 |
Golgi | – | 0 | 0 | 0 | 0 | 0 | 2 | 5 | 0 | 4 | 1 | 8 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | – | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 3 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 1 | 1 |
Nucleus | – | 1 | 1 | 1 | 0 | 4 | 1 | 10 | 4 | 5 | 2 | 10 | 0 | 0 | 0 | – | – | – | 0 | 3 | 2 | 3 | 8 | 4 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 1 | 1 | 1 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 2 | 3 | 2 | 7 | 13 | 40 | 33 | 14 | 37 | 19 | 52 | 0 | 0 | 0 | – | – | – | 1 | 1 | 0 | 2 | 5 | 2 |
Unique Cell Count | 159 | 173 | 120 | 258 | 247 | 259 | 306 | 237 | 266 | 228 | 314 | 96 | 125 | 156 | 52 | 158 | 161 | 67 | 208 | 229 | ||||
Labelled Cell Count | 160 | 182 | 123 | 262 | 256 | 271 | 325 | 245 | 275 | 235 | 333 | 96 | 125 | 157 | 52 | 158 | 161 | 67 | 208 | 229 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 28.4 | 28.6 | 29.6 | 29.4 | 23.9 | 22.9 | 21.4 | 19.3 | 20.1 | 19.4 | 20.9 | 31.7 | 29.9 | 29.6 | – | – | – | 17.3 | 23.9 | 24.7 |
Std Deviation (1e-4) | – | 6.0 | 6.5 | 6.4 | 5.4 | 5.1 | 5.0 | 4.9 | 4.5 | 4.3 | 4.8 | 5.4 | 6.9 | 6.2 | 6.7 | – | – | – | 7.8 | 9.2 | 9.3 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.04 | -0.26 | -0.32 | -0.42 | -0.57 | -0.51 | -0.56 | -0.46 | 0.15 | 0.06 | 0.05 | – | – | – | -0.72 | -0.26 | -0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0.1 | -0.5 | -0.3 | 0 | 0.4 | 0 | 0.5 | 0 | 0 | 0 | – | – | – |
Cytoplasm | -1.3 | -0.9 | -3.5 | -5.3 | -5.4 | -4.2 | -6.0 | -5.2 | -7.2 | 1.3 | 1.5 | 1.7 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2.5 | 1.8 | 0 | 2.5 | 2.3 | 0 | 0 | 0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 0 | 1.9 | 4.7 | 3.6 | 2.1 | 4.3 | 2.9 | 4.9 | 0 | 0 | 0 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.5683 | 23.9293 | 22.5795 | 21.4202 | 19.1663 | 21.8579 | 17.998 | 21.9246 | 18.5828 | 18.5872 | 19.9689 | 18.3693 | 19.3157 | 23.5487 | 21.3904 | 20.5242 | 17.1181 | 21.2822 |
Actin | 0.0234 | 0.0002 | 0.0031 | 0.0003 | 0.0142 | 0.0006 | 0.0223 | 0.0001 | 0.0049 | 0.0001 | 0.0068 | 0.0027 | 0.0028 | 0.0004 | 0.0032 | 0.0003 | 0.0049 | 0.0004 |
Bud | 0.0014 | 0.0001 | 0.0012 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0004 | 0 |
Bud Neck | 0.0008 | 0.0002 | 0.0011 | 0.0004 | 0.0009 | 0.0009 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0003 | 0.0006 | 0.0005 | 0.0001 | 0.0002 | 0.0003 | 0.0008 | 0.0004 |
Bud Periphery | 0.0014 | 0 | 0.0015 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 |
Bud Site | 0.0036 | 0.0007 | 0.0042 | 0.0002 | 0.0018 | 0 | 0.0003 | 0 | 0.0003 | 0 | 0.0004 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0016 | 0 |
Cell Periphery | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Cytoplasm | 0.8343 | 0.9744 | 0.935 | 0.9441 | 0.7411 | 0.944 | 0.8976 | 0.9951 | 0.9073 | 0.9841 | 0.9369 | 0.9544 | 0.915 | 0.9927 | 0.9608 | 0.9128 | 0.8981 | 0.9414 |
Cytoplasmic Foci | 0.014 | 0.0008 | 0.006 | 0.0087 | 0.0403 | 0.0034 | 0.0024 | 0.0003 | 0.0095 | 0.0003 | 0.0349 | 0.0013 | 0.0023 | 0.0008 | 0.003 | 0.0054 | 0.0331 | 0.0014 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0068 | 0.0003 | 0.0015 | 0.0022 | 0.0088 | 0.003 | 0.0134 | 0.0001 | 0.0024 | 0.0002 | 0.0018 | 0.0041 | 0.0042 | 0.0001 | 0.0001 | 0.0011 | 0.0014 | 0.0005 |
Endosome | 0.0128 | 0.0003 | 0.006 | 0.0134 | 0.0442 | 0.0022 | 0.0095 | 0 | 0.0093 | 0.0001 | 0.0027 | 0.0055 | 0.004 | 0.0001 | 0.0011 | 0.003 | 0.0091 | 0.0116 |
Golgi | 0.0075 | 0.0001 | 0.0021 | 0.0011 | 0.0282 | 0.0001 | 0.0015 | 0 | 0.0066 | 0 | 0.0009 | 0.0056 | 0.0033 | 0 | 0.002 | 0.0003 | 0.0039 | 0.003 |
Lipid Particles | 0.0106 | 0 | 0.0024 | 0.0076 | 0.0151 | 0.0001 | 0.0007 | 0 | 0.0021 | 0 | 0.0011 | 0.0003 | 0.0061 | 0 | 0.0074 | 0.0057 | 0.0079 | 0.0106 |
Mitochondria | 0.0092 | 0.0001 | 0.0079 | 0.0008 | 0.0127 | 0.0002 | 0.0009 | 0 | 0.0101 | 0.0001 | 0.0002 | 0.0014 | 0.005 | 0 | 0.0014 | 0.0013 | 0.015 | 0.0003 |
None | 0.0033 | 0.0022 | 0.0007 | 0.0027 | 0.0072 | 0.0006 | 0.0043 | 0.0002 | 0.0006 | 0.0002 | 0.0017 | 0.0013 | 0.0012 | 0.0002 | 0.0002 | 0.0014 | 0.0008 | 0.0003 |
Nuclear Periphery | 0.0144 | 0.0013 | 0.0029 | 0.002 | 0.0071 | 0.0063 | 0.0218 | 0.0002 | 0.0114 | 0.0014 | 0.0012 | 0.0031 | 0.0054 | 0.0005 | 0.0008 | 0.0185 | 0.0015 | 0.0035 |
Nucleolus | 0.001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0135 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0039 | 0.0002 | 0 |
Nucleus | 0.0358 | 0.0187 | 0.0187 | 0.0122 | 0.0248 | 0.0361 | 0.0208 | 0.0035 | 0.0157 | 0.0128 | 0.0084 | 0.0178 | 0.0451 | 0.0048 | 0.0107 | 0.0314 | 0.0128 | 0.0247 |
Peroxisomes | 0.0084 | 0 | 0.0029 | 0.0003 | 0.0417 | 0.0001 | 0.0002 | 0 | 0.0019 | 0 | 0.0007 | 0 | 0.0019 | 0.0001 | 0.0084 | 0.0004 | 0.0057 | 0.0002 |
Punctate Nuclear | 0.0067 | 0.0002 | 0.0005 | 0.0004 | 0.0033 | 0.0011 | 0.0016 | 0.0001 | 0.0015 | 0.0001 | 0.0015 | 0.0005 | 0.0007 | 0.0001 | 0.0003 | 0.0099 | 0.0011 | 0.0006 |
Vacuole | 0.0026 | 0.0003 | 0.0013 | 0.0025 | 0.005 | 0.0008 | 0.001 | 0 | 0.0009 | 0.0001 | 0.0002 | 0.0007 | 0.0008 | 0.0001 | 0.0001 | 0.0016 | 0.0009 | 0.0004 |
Vacuole Periphery | 0.001 | 0 | 0.0004 | 0.0006 | 0.0019 | 0.0002 | 0.001 | 0 | 0.0009 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0 | 0 | 0.0024 | 0.0004 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 258.9623 | 262.81 | 274.6826 | 283.1757 | 264.3875 | 233.8122 | 245.0907 | 267.4953 | 262.1306 | 259.1259 |
Translational Efficiency | 1.3972 | 1.3822 | 1.326 | 1.447 | 1.3434 | 1.5075 | 1.579 | 1.3845 | 1.45 | 1.4264 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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