Standard name
Human Ortholog
Description Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.06 0 0 0 0 0 0 0 0 0.06
Cytoplasm 0.67 0.59 0.72 0.72 0.75 0.82 0.87 0.89 0.88 0.87 0.89 0.89 0.92 0.93 0.98 0.19 0.24 0.26 0.11 0.08 0.11 0.14 0.13 0.11
Endoplasmic Reticulum 0.11 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0.05 0.06 0.13 0.16 0.26
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0.07 0.06 0 0.13 0 0.05 0 0 0 0 0 0 0 0 0 0.38 0.36 0.28 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.31 0.28 0.3 0.27 0.19 0.12 0.08 0.1 0.11 0.08 0.12 0 0 0 0.63 0.64 0.59 0.71 0.72 0.63 0.55 0.52 0.4
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 0 1 1 4 1 1 3 0 1 2 5 5 2 1 1 3 3 3 9 8 11
Bud 1 4 1 0 0 2 6 10 13 16 19 12 0 1 0 0 0 0 3 9 1 2 4 5
Bud Neck 0 0 0 0 0 0 0 1 0 2 0 0 1 1 1 0 0 0 2 9 3 6 10 18
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 3 0 4 6 7 9 10 8 5 21 14 3 2 6 1 0 1 3 7 18
Cytoplasm 100 154 68 102 227 203 230 278 265 339 205 282 120 165 214 103 88 112 23 26 17 22 26 35
Endoplasmic Reticulum 17 23 1 5 9 8 3 9 6 12 2 6 5 3 0 13 18 17 9 16 10 21 32 79
Endosome 0 5 0 0 4 0 1 1 0 0 0 4 1 0 1 8 4 9 1 9 3 1 1 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 7 6 3 0 1 0 0 1 1
Mitochondria 10 1 1 1 1 5 2 3 10 7 12 6 0 0 0 4 0 7 8 5 11 6 5 8
Nucleus 11 16 4 19 9 12 4 6 4 4 1 1 2 1 0 204 130 118 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 2 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 1 1 0 0 0 1 0 0 1 3 0 0 0 2 1 2 0 0 0 0
Vac/Vac Membrane 19 81 26 42 81 46 33 26 31 42 18 39 0 0 5 342 229 248 143 227 100 93 109 125
Unique Cell Count 150 262 94 141 304 248 265 311 301 389 230 318 130 178 219 542 360 423 203 315 160 168 210 312
Labelled Cell Count 159 286 102 169 336 280 287 341 337 436 268 361 138 200 240 686 478 524 203 315 160 168 210 312


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.9 6.2 6.8 6.9 6.2 5.9 6.5 5.7 6.4 5.7 6.2 6.5 6.6 6.8 9.2 9.7 9.8 5.5 6.8 7.4
Std Deviation (1e-4) 0.9 1.6 1.4 2.1 1.9 1.7 1.5 1.7 1.3 1.8 1.3 1.3 1.3 1.6 1.8 2.5 2.3 2.6 1.6 1.8 1.7
Intensity Change (Log2) – – – 0.14 0.17 0.01 -0.07 0.08 -0.12 0.05 -0.12 0.01 0.07 0.09 0.14 0.58 0.66 0.67 -0.15 0.14 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 2.4 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 3.5 0 0 0 0
Cytoplasm 0 0.5 1.9 3.2 4.1 3.7 3.5 3.8 3.9 4.0 4.6 6.9 -10.8 -8.7 -8.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 2.3 0 0 0 0 0 0 0 0 0 0 0 6.4 6.0 4.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.4 -0.2 -1.8 -3.4 -4.9 -4.2 -4.2 -4.7 -3.6 -6.4 -7.4 -6.9 6.4 6.3 5.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1851 2.0038 2.2277 1.925 2.1534 2.055 0.2296 1.2749 1.0248 0.6626 0.8552 1.0417 0.7485 0.7416 0.4853 1.4522 0.3587 0.3359
Actin 0.0182 0.0024 0.0085 0.0086 0.0058 0.0017 0.0167 0.0003 0.0012 0.0003 0.0017 0.0004 0.0507 0.0079 0.0226 0.0135 0.0016 0.0066
Bud 0.0019 0.0031 0.0026 0.0046 0.0029 0.0014 0.0019 0.0027 0.009 0.002 0.0061 0.0009 0.0026 0.0023 0.0031 0.0036 0.0034 0.0008
Bud Neck 0.0019 0.0022 0.0021 0.01 0.0058 0.0092 0.0045 0.004 0.0101 0.0019 0.0011 0.0093 0.008 0.0016 0.0022 0.0045 0.0025 0.0198
Bud Periphery 0.0037 0.0079 0.0088 0.0131 0.0081 0.0043 0.0044 0.0046 0.0192 0.0095 0.0075 0.0019 0.0073 0.0039 0.0075 0.0074 0.0114 0.0053
Bud Site 0.0141 0.0472 0.0319 0.0171 0.011 0.0024 0.0166 0.0322 0.048 0.0046 0.0028 0.0013 0.025 0.06 0.0103 0.0092 0.0042 0.0013
Cell Periphery 0.0081 0.0071 0.0049 0.0037 0.0044 0.0033 0.0036 0.0058 0.0041 0.0063 0.002 0.0018 0.0049 0.0061 0.0039 0.0014 0.0029 0.0028
Cytoplasm 0.2806 0.3635 0.403 0.3006 0.384 0.4338 0.2947 0.3594 0.3761 0.4512 0.3884 0.418 0.3928 0.4216 0.4662 0.4028 0.4905 0.5098
Cytoplasmic Foci 0.0579 0.0272 0.0283 0.0537 0.0429 0.0301 0.0457 0.0201 0.0359 0.0487 0.0427 0.0425 0.0552 0.0259 0.0287 0.0517 0.0396 0.0416
Eisosomes 0.0007 0.0004 0.0002 0.0002 0.0001 0.0002 0.0004 0.0001 0.0001 0.0001 0.0001 0 0.0008 0.0002 0.0002 0.0005 0.0001 0.0001
Endoplasmic Reticulum 0.013 0.0045 0.0076 0.0058 0.0088 0.0048 0.008 0.0089 0.0086 0.0063 0.0048 0.0057 0.0124 0.0098 0.0086 0.0099 0.0071 0.006
Endosome 0.0763 0.0346 0.0599 0.1106 0.0613 0.0555 0.0464 0.0345 0.0599 0.0532 0.076 0.0619 0.0928 0.0362 0.0481 0.096 0.0788 0.0501
Golgi 0.0102 0.0044 0.0101 0.0132 0.0127 0.0046 0.0027 0.0011 0.0017 0.0014 0.0019 0.0014 0.0156 0.0041 0.0048 0.0196 0.007 0.0074
Lipid Particles 0.0226 0.0025 0.0071 0.006 0.0182 0.0084 0.0097 0.0039 0.0043 0.0027 0.0084 0.0018 0.0196 0.0084 0.004 0.0102 0.0198 0.0103
Mitochondria 0.0082 0.0033 0.0155 0.0067 0.069 0.0076 0.0031 0.0009 0.0014 0.0013 0.0016 0.0008 0.0277 0.0013 0.0071 0.0514 0.0015 0.0085
None 0.3824 0.4284 0.3424 0.3508 0.2409 0.3367 0.4496 0.379 0.3232 0.3215 0.3241 0.2976 0.1791 0.3382 0.3125 0.1836 0.2172 0.2603
Nuclear Periphery 0.0108 0.0009 0.0022 0.0019 0.0021 0.0021 0.0072 0.002 0.0019 0.0016 0.0025 0.0015 0.0119 0.0017 0.0016 0.003 0.0012 0.0038
Nucleolus 0.002 0.0012 0.001 0.0032 0.0007 0.0011 0.0022 0.0017 0.0023 0.0009 0.0041 0.001 0.0014 0.0008 0.0005 0.0011 0.0005 0.0004
Nucleus 0.0124 0.0032 0.0032 0.0054 0.002 0.004 0.0052 0.0069 0.0055 0.0035 0.0056 0.0046 0.0178 0.0031 0.0022 0.0354 0.0032 0.0021
Peroxisomes 0.0118 0.0012 0.007 0.0067 0.0236 0.0031 0.0036 0.0004 0.0009 0.0008 0.0043 0.0007 0.01 0.0016 0.0072 0.0023 0.0051 0.003
Punctate Nuclear 0.0086 0.0022 0.0023 0.0018 0.0009 0.0043 0.0039 0.0025 0.003 0.0018 0.0134 0.002 0.0033 0.0014 0.0008 0.0028 0.001 0.0022
Vacuole 0.0513 0.0514 0.0491 0.0737 0.0909 0.0786 0.0672 0.1263 0.0813 0.0776 0.0965 0.1414 0.0565 0.062 0.0555 0.087 0.0989 0.0558
Vacuole Periphery 0.0033 0.0013 0.0023 0.0027 0.0036 0.0028 0.003 0.0026 0.0024 0.0028 0.0046 0.0036 0.0045 0.0018 0.0023 0.0031 0.0026 0.0019

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 66.7341 47.3606 27.0336 32.9979 43.5921 75.3525 51.1094 45.6831 45.5836 58.1208
Translational Efficiency 1.0423 0.9016 0.713 0.9821 1.0338 0.959 0.8377 0.7958 0.7791 0.8855

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
Localization
Cell Percentages vacuole (73%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Opy2

Opy2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Opy2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available