Standard name
Human Ortholog
Description Mitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-ancor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.3 0.16 0.2 0.13 0 0.08 0.14 0.05 0 0 0.1 0.07 0.07 0.42 0.35 0.3 0.18 0.2 0.14 0.08 0.14 0.16
Bud 0.07 0.06 0 0.13 0 0 0 0.07 0.06 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0.05 0.05 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.12 0.27 0.2 0 0.09 0 0 0 0 0 0 0.21 0.18 0.15 0.3 0.26 0.36 0.23 0.26 0.23 0.21 0.31 0.28
Cytoplasm 0.63 0.55 0.63 0.73 0.77 0.83 0.71 0.37 0.58 0.35 0.5 0.82 0.86 0.91 0.3 0.31 0.35 0.14 0.13 0.13 0.3 0.22 0.24
Endoplasmic Reticulum 0 0.07 0.05 0 0 0.05 0.09 0.05 0.11 0.05 0.06 0 0 0 0.08 0.07 0.07 0.16 0.13 0.1 0.05 0.07 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0.16 0 0.09 0.14 0.65 0.35 0.71 0.63 0 0 0 0.06 0 0 0.15 0.12 0.31 0.2 0.13 0.11
Nucleus 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
SpindlePole 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.08 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 41 86 12 11 11 1 14 6 7 2 3 29 46 48 119 102 92 73 79 18 14 38 51
Bud 21 16 0 7 2 3 7 3 9 5 3 7 3 2 17 8 7 4 3 0 0 2 3
Bud Neck 1 0 1 0 1 1 2 0 1 0 0 0 2 2 0 2 0 21 30 3 9 12 15
Bud Site 1 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2 0
Cell Periphery 35 78 15 2 7 4 4 0 4 1 0 61 111 111 86 75 110 93 101 29 38 84 91
Cytoplasm 188 159 48 40 63 99 124 16 87 40 42 243 546 664 87 89 105 58 50 16 55 60 77
Endoplasmic Reticulum 7 20 4 1 3 6 16 2 16 6 5 7 7 5 24 21 22 64 51 12 9 19 27
Endosome 4 2 0 0 0 1 1 0 2 0 0 1 5 3 2 6 1 3 5 2 2 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 7 3 1 3 0 0 0 0
Mitochondria 40 6 0 9 1 11 24 28 53 81 53 0 0 1 16 12 8 61 47 39 36 35 36
Nucleus 4 2 0 4 0 1 1 0 2 0 1 0 2 4 3 1 1 0 0 0 1 0 0
Nuclear Periphery 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 7 1 0 0 0 0 0 0 0 0 0 0 7 2 4 21 3 2 1 0 0 0 0
SpindlePole 14 3 1 0 3 3 4 1 2 2 1 7 18 18 20 28 23 0 0 0 2 1 1
Vac/Vac Membrane 1 2 0 0 1 2 0 0 1 0 0 2 0 2 3 11 6 12 14 2 7 9 12
Unique Cell Count 299 289 76 55 82 119 174 43 150 114 84 295 632 731 286 289 302 403 393 130 182 272 325
Labelled Cell Count 364 376 81 75 92 132 197 56 184 137 108 358 748 862 385 385 381 403 393 130 182 272 325


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 6.0 6.0 5.2 6.2 5.0 4.9 4.0 4.6 4.1 4.4 6.4 6.3 6.2 7.5 7.8 7.2 6.0 6.1 6.2
Std Deviation (1e-4) 0.8 0.7 0.7 1.0 1.3 1.6 0.9 0.8 1.0 1.2 0.8 1.2 1.2 1.1 1.1 1.4 1.1 1.3 1.3 1.1
Intensity Change (Log2) -0.22 0.04 -0.26 -0.3 -0.6 -0.38 -0.56 -0.47 0.09 0.05 0.02 0.31 0.36 0.25 -0.02 0.01 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 -0.4 -4.1 -1.8 0 0 0 0 -1.5 -2.6 -2.9 4.2 3.3 2.6
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.7 -2.0 -3.8 -4.8 0 0 0 0 0.2 -0.5 -1.0 1.8 1.1 2.8
Cytoplasm 1.2 1.9 3.2 1.3 -2.7 -0.7 -3.8 -1.7 3.6 5.2 7.1 -5.2 -5.2 -4.5
Endoplasmic Reticulum 0 0 0 1.1 0 0 0 0 0 0 0 0.9 0.6 0.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.4 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.7353 0.9176 0.8628 0.4102 0.6817 0.7612 1.227 1.1859 1.3621 0.9971 1.0141 1.0666 1.7622 1.9038 1.8354 1.9232 1.857 1.8056
Actin 0.0989 0.0587 0.0988 0.1124 0.1401 0.0562 0.0829 0.0983 0.0528 0.0792 0.0773 0.0612 0.103 0.0453 0.0774 0.1001 0.012 0.0669
Bud 0.006 0.0034 0.0056 0.0038 0.0086 0.0042 0.0031 0.0033 0.0038 0.0028 0.0126 0.0044 0.004 0.0052 0.0066 0.0032 0.0155 0.0059
Bud Neck 0.0334 0.0069 0.0257 0.0213 0.0575 0.0338 0.023 0.0098 0.0192 0.0244 0.0306 0.0227 0.0242 0.0051 0.0342 0.0222 0.0054 0.03
Bud Periphery 0.0289 0.0077 0.0168 0.0261 0.0347 0.0315 0.0119 0.0126 0.021 0.023 0.0341 0.037 0.0165 0.0062 0.0167 0.011 0.0472 0.0313
Bud Site 0.063 0.144 0.0518 0.0227 0.0189 0.0134 0.0282 0.1838 0.0621 0.0219 0.0375 0.0082 0.0354 0.168 0.0744 0.0713 0.0212 0.0147
Cell Periphery 0.0866 0.035 0.056 0.0196 0.0406 0.0563 0.0843 0.0656 0.074 0.103 0.0557 0.0808 0.0588 0.05 0.0513 0.0477 0.0153 0.0732
Cytoplasm 0.2882 0.4155 0.3364 0.3113 0.258 0.4446 0.3814 0.3744 0.4863 0.4491 0.3436 0.4851 0.4198 0.5092 0.4857 0.4572 0.4344 0.533
Cytoplasmic Foci 0.0359 0.0378 0.0354 0.106 0.0602 0.0461 0.0305 0.0222 0.022 0.014 0.0517 0.0312 0.048 0.0318 0.0417 0.044 0.1187 0.0344
Eisosomes 0.0186 0.01 0.0102 0.0048 0.0059 0.0036 0.0164 0.0136 0.009 0.0193 0.0065 0.0121 0.0165 0.0058 0.0109 0.0049 0.0012 0.0099
Endoplasmic Reticulum 0.0105 0.0102 0.0099 0.0056 0.0098 0.0062 0.0135 0.0045 0.006 0.0227 0.0049 0.0081 0.0067 0.0073 0.0089 0.0126 0.0036 0.005
Endosome 0.0228 0.0304 0.0204 0.0355 0.0257 0.016 0.0189 0.005 0.007 0.0062 0.0391 0.008 0.0167 0.0102 0.0094 0.018 0.032 0.0068
Golgi 0.0141 0.0225 0.0146 0.0311 0.0274 0.01 0.0104 0.0115 0.0075 0.0116 0.0175 0.0074 0.0138 0.0134 0.009 0.008 0.0059 0.0085
Lipid Particles 0.0123 0.0031 0.0055 0.0077 0.0199 0.0053 0.0068 0.0017 0.0029 0.0192 0.0283 0.0031 0.0081 0.0017 0.0052 0.0031 0.0331 0.0102
Mitochondria 0.0104 0.0069 0.014 0.0047 0.0407 0.0108 0.0074 0.0098 0.0103 0.0185 0.0153 0.0086 0.0101 0.0075 0.0177 0.0018 0.0081 0.0114
None 0.2139 0.197 0.2691 0.2654 0.1794 0.2456 0.2511 0.174 0.197 0.1635 0.1821 0.2056 0.1774 0.1242 0.1164 0.1378 0.1573 0.1363
Nuclear Periphery 0.0037 0.0007 0.0021 0.0006 0.0146 0.0004 0.0036 0.0003 0.0012 0.0005 0.0037 0.0023 0.0095 0.0003 0.0008 0.0014 0.0023 0.0006
Nucleolus 0.0098 0.0009 0.0017 0.0006 0.0057 0.0007 0.0031 0.0006 0.001 0.002 0.0041 0.0007 0.0038 0.0004 0.0047 0.0024 0.0085 0.001
Nucleus 0.0061 0.0016 0.0025 0.0011 0.0081 0.0011 0.0052 0.001 0.0016 0.0015 0.006 0.0027 0.009 0.0008 0.0029 0.0364 0.0047 0.002
Peroxisomes 0.0166 0.0019 0.0139 0.0148 0.0178 0.0065 0.0049 0.0047 0.0027 0.0127 0.0174 0.0036 0.0068 0.0043 0.0097 0.006 0.0094 0.0147
Punctate Nuclear 0.0095 0.0011 0.0035 0.0013 0.0182 0.0012 0.0071 0.0005 0.0034 0.0005 0.0219 0.0033 0.002 0.0004 0.0037 0.0057 0.0318 0.0019
Vacuole 0.0097 0.0041 0.005 0.0028 0.0055 0.0056 0.0055 0.0026 0.0085 0.004 0.009 0.0033 0.008 0.0026 0.0116 0.0049 0.0282 0.0019
Vacuole Periphery 0.0012 0.0005 0.0012 0.0005 0.0026 0.0006 0.0008 0.0002 0.0005 0.0005 0.0012 0.0004 0.0018 0.0002 0.0011 0.0003 0.0041 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8292 5.6688 4.6272 6.153 5.7553 5.7041 7.0214 7.5437 5.3513 7.1811
Translational Efficiency 0.3476 0.6576 0.6573 0.5574 0.4729 0.945 0.6509 0.5873 0.6688 0.6652

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-ancor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission
Localization
Cell Percentages ER (15%), cell periphery (11%), mixed (41%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mdm36

Mdm36


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mdm36-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available