ORF | |
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Human Ortholog | |
Description | Protein that contains a PX domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; PX stands for Phox homology |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 3 | 5 |
Bud | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 5 | 2 | – | 11 | 5 | 0 | 0 | 5 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 7 | 5 |
Bud Neck | 2 | 0 | 3 | 1 | 1 | 3 | 9 | 12 | 2 | – | 7 | 10 | 0 | 0 | 0 | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 2 | – | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 1 | 2 | 1 | 3 | 2 |
Cytoplasm | 258 | 248 | 179 | 205 | 305 | 306 | 393 | 447 | 274 | – | 204 | 262 | 314 | 413 | 440 | 211 | 146 | 173 | 219 | 212 | 98 | 182 | 258 | 284 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 14 | 4 | 17 | 1 | 0 | 1 | 0 | 1 | 4 |
Endosome | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 4 | 1 | 2 | 0 | 3 | 3 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 15 | 2 | 12 | 7 | 34 | 69 | 80 | 85 | – | 136 | 176 | 1 | 0 | 11 | 14 | 3 | 2 | 38 | 22 | 38 | 10 | 9 | 15 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 1 | – | 2 | 0 | 1 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 8 | 6 | – | 7 | 11 | 0 | 0 | 0 | 2 | 1 | 2 | 4 | 2 | 2 | 0 | 1 | 2 |
Vac/Vac Membrane | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 2 | 5 | 2 | 2 | 4 | 5 | 1 | 5 | 5 |
Unique Cell Count | 264 | 257 | 179 | 207 | 309 | 318 | 430 | 487 | 311 | 292 | 361 | 314 | 413 | 442 | 223 | 154 | 184 | 284 | 255 | 161 | 203 | 308 | 346 | |
Labelled Cell Count | 269 | 270 | 185 | 222 | 317 | 344 | 483 | 555 | 375 | 374 | 468 | 316 | 414 | 460 | 251 | 165 | 206 | 284 | 255 | 161 | 203 | 308 | 346 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 3.1 | 2.9 | 2.9 | 3.2 | 2.9 | 2.5 | 3.0 | 2.4 | – | 2.3 | 2.0 | 3.9 | 4.0 | 3.5 | 5.2 | 5.6 | 5.5 | 3.8 | 3.9 | 4.1 |
Std Deviation (1e-4) | 0.8 | 0.4 | 0.7 | 1.5 | 1.7 | 1.4 | 0.8 | 1.5 | 1.2 | – | 1.1 | 0.8 | 1.2 | 1.0 | 1.1 | 1.8 | 2.3 | 2.4 | 0.8 | 1.6 | 0.9 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.19 | 0.02 | -0.17 | 0.05 | -0.28 | – | -0.29 | -0.51 | 0.46 | 0.5 | 0.3 | 0.86 | 0.97 | 0.95 | 0.43 | 0.46 | 0.52 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.0843 | 0.0898 | 0.0802 | -0.4811 | -0.109 | -0.0198 | 0.6505 | 0.7022 | 0.619 | 0.4881 | 0.9066 | 0.4841 | 0.0703 | 0.0919 | 0.4436 | 0.607 | 0.2717 | 0.8606 |
Actin | 0.0289 | 0.0001 | 0.0412 | 0 | 0.0242 | 0.0007 | 0.0324 | 0.0003 | 0.0021 | 0.0082 | 0.0095 | 0.0022 | 0.002 | 0 | 0.0003 | 0.0001 | 0 | 0.0003 |
Bud | 0.0015 | 0.0088 | 0.0003 | 0 | 0.0003 | 0.0003 | 0.002 | 0.0038 | 0.0009 | 0.0031 | 0.0012 | 0.0007 | 0.0001 | 0 | 0.0005 | 0 | 0 | 0 |
Bud Neck | 0.0071 | 0.0001 | 0.005 | 0 | 0.0003 | 0.0006 | 0.0028 | 0.0001 | 0.0001 | 0.0025 | 0.0001 | 0.0011 | 0.0001 | 0 | 0 | 0 | 0 | 0.0003 |
Bud Periphery | 0.0038 | 0.0012 | 0.0004 | 0 | 0.0006 | 0.0005 | 0.0032 | 0.0024 | 0.0008 | 0.004 | 0.0014 | 0.0009 | 0.0001 | 0 | 0.001 | 0 | 0 | 0.0001 |
Bud Site | 0.0169 | 0.0007 | 0.0031 | 0 | 0.0004 | 0.0003 | 0.0161 | 0.0127 | 0.0049 | 0.0282 | 0.0004 | 0.0005 | 0.0003 | 0 | 0.0006 | 0 | 0 | 0 |
Cell Periphery | 0.0005 | 0.0002 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0011 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0 | 0 |
Cytoplasm | 0.0455 | 0.0421 | 0.035 | 0.0109 | 0.0735 | 0.0855 | 0.0442 | 0.0653 | 0.078 | 0.0512 | 0.0781 | 0.1257 | 0.0266 | 0.0207 | 0.016 | 0.0081 | 0.0102 | 0.0131 |
Cytoplasmic Foci | 0.0239 | 0.0033 | 0.0074 | 0.0008 | 0.0046 | 0.0041 | 0.0191 | 0.0106 | 0.013 | 0.0105 | 0.0075 | 0.0261 | 0.0038 | 0.0006 | 0.0019 | 0.0007 | 0.0005 | 0.0021 |
Eisosomes | 0.0005 | 0.0002 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0001 | 0.0003 |
Endoplasmic Reticulum | 0.0046 | 0.0019 | 0.0012 | 0.0001 | 0.0054 | 0.0013 | 0.0085 | 0.0004 | 0.0009 | 0.0013 | 0.0013 | 0.0013 | 0.0006 | 0.0002 | 0.0012 | 0.0002 | 0.0001 | 0.0001 |
Endosome | 0.0152 | 0.0054 | 0.0048 | 0.0001 | 0.016 | 0.0011 | 0.0188 | 0.0003 | 0.003 | 0.008 | 0.0383 | 0.0143 | 0.0009 | 0 | 0.0006 | 0 | 0 | 0.0006 |
Golgi | 0.006 | 0.0003 | 0.0032 | 0 | 0.004 | 0.0001 | 0.006 | 0.0001 | 0.0022 | 0.0043 | 0.0215 | 0.0064 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0011 |
Lipid Particles | 0.0125 | 0.0008 | 0.0068 | 0 | 0.0029 | 0.0002 | 0.0106 | 0.0004 | 0.0014 | 0.0064 | 0.0132 | 0.0041 | 0.0002 | 0 | 0.0007 | 0.0001 | 0 | 0.0002 |
Mitochondria | 0.004 | 0.0014 | 0.0015 | 0 | 0.0019 | 0.0003 | 0.0192 | 0.0004 | 0.0034 | 0.0215 | 0.0088 | 0.0046 | 0.0002 | 0 | 0.0005 | 0.0001 | 0 | 0.0141 |
None | 0.763 | 0.9296 | 0.8681 | 0.9877 | 0.8249 | 0.8988 | 0.7387 | 0.8951 | 0.8815 | 0.8308 | 0.7702 | 0.7961 | 0.9542 | 0.9777 | 0.9744 | 0.9899 | 0.9888 | 0.9665 |
Nuclear Periphery | 0.0173 | 0.0004 | 0.0011 | 0.0001 | 0.0308 | 0.0014 | 0.0335 | 0.0003 | 0.0006 | 0.0017 | 0.0134 | 0.0014 | 0.0013 | 0 | 0.0002 | 0.0001 | 0 | 0.0001 |
Nucleolus | 0.0013 | 0.0003 | 0.0009 | 0 | 0.0003 | 0.0003 | 0.003 | 0.0004 | 0.0002 | 0.0015 | 0.0008 | 0.0018 | 0.0003 | 0 | 0.0003 | 0 | 0 | 0.0001 |
Nucleus | 0.0237 | 0.0013 | 0.0014 | 0 | 0.003 | 0.0024 | 0.0137 | 0.0008 | 0.0007 | 0.0019 | 0.0174 | 0.0041 | 0.0022 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 |
Peroxisomes | 0.0081 | 0.0001 | 0.0128 | 0 | 0.0006 | 0.0001 | 0.0033 | 0.0002 | 0.0025 | 0.0072 | 0.0069 | 0.0034 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0.0004 |
Punctate Nuclear | 0.0125 | 0.0005 | 0.0041 | 0.0001 | 0.0043 | 0.0006 | 0.0155 | 0.0052 | 0.0021 | 0.0039 | 0.0018 | 0.0032 | 0.0061 | 0 | 0.0002 | 0.0001 | 0 | 0.0003 |
Vacuole | 0.0023 | 0.0012 | 0.0008 | 0.0001 | 0.0008 | 0.0012 | 0.0047 | 0.0007 | 0.0013 | 0.0025 | 0.0043 | 0.0012 | 0.0003 | 0.0001 | 0.0004 | 0 | 0 | 0.0001 |
Vacuole Periphery | 0.0009 | 0.0002 | 0.0002 | 0 | 0.001 | 0.0002 | 0.0028 | 0.0001 | 0.0002 | 0.0006 | 0.0035 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.9926 | 19.2867 | 32.2578 | 28.0749 | 24.4208 | 32.8579 | 22.2092 | 27.4724 | 23.8825 | 33.5552 |
Translational Efficiency | 0.9482 | 0.9151 | 0.6117 | 0.6743 | 0.8256 | 0.7748 | 0.8776 | 0.5366 | 0.79 | 0.6821 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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