Standard name | |
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Human Ortholog | |
Description | Mitochondrial ribosomal protein of the large subunit |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 1 | 1 | 1 | 1 | 0 | – | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | 0 | 5 | 6 | 1 | 3 | 6 | 18 |
Bud | 3 | 0 | 14 | 5 | 8 | 8 | – | 20 | 15 | 13 | 15 | 22 | 6 | 3 | 2 | – | – | 0 | 1 | 0 | 0 | 1 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 1 | 1 | 1 | 2 |
Bud Site | 0 | 0 | 4 | 0 | 1 | 2 | – | 6 | 8 | 2 | 2 | 12 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 7 | 5 | 11 | 9 | 1 | 4 | – | 3 | 2 | 7 | 2 | 5 | 8 | 12 | 7 | – | – | 2 | 0 | 0 | 0 | 0 | 1 | 2 |
Cytoplasm | 1 | 5 | 4 | 7 | 14 | 13 | – | 13 | 8 | 8 | 5 | 8 | 7 | 13 | 11 | – | – | 4 | 0 | 0 | 0 | 0 | 4 | 1 |
Endoplasmic Reticulum | 8 | 2 | 1 | 3 | 2 | 5 | – | 6 | 1 | 9 | 4 | 5 | 2 | 4 | 4 | – | – | 1 | 0 | 0 | 0 | 1 | 1 | 2 |
Endosome | 8 | 2 | 2 | 0 | 12 | 4 | – | 11 | 0 | 1 | 1 | 0 | 13 | 17 | 22 | – | – | 1 | 24 | 5 | 19 | 5 | 15 | 20 |
Golgi | 12 | 13 | 1 | 0 | 1 | 0 | – | 1 | 1 | 1 | 0 | 1 | 18 | 31 | 41 | – | – | 2 | 27 | 12 | 11 | 12 | 37 | 28 |
Mitochondria | 388 | 139 | 282 | 324 | 377 | 426 | – | 752 | 562 | 691 | 495 | 629 | 317 | 376 | 396 | – | – | 1 | 346 | 122 | 231 | 130 | 229 | 267 |
Nucleus | 4 | 0 | 0 | 0 | 3 | 5 | – | 9 | 4 | 1 | 2 | 1 | 1 | 0 | 0 | – | – | 1 | 2 | 1 | 1 | 1 | 0 | 1 |
Nuclear Periphery | 26 | 8 | 16 | 22 | 23 | 49 | – | 57 | 69 | 83 | 58 | 80 | 15 | 20 | 14 | – | – | 3 | 5 | 2 | 2 | 1 | 5 | 4 |
Nucleolus | 13 | 4 | 13 | 8 | 20 | 50 | – | 69 | 54 | 77 | 58 | 53 | 19 | 11 | 7 | – | – | 0 | 1 | 1 | 0 | 1 | 4 | 2 |
Peroxisomes | 5 | 2 | 5 | 4 | 13 | 6 | – | 23 | 4 | 4 | 0 | 6 | 17 | 22 | 29 | – | – | 0 | 14 | 4 | 24 | 13 | 21 | 23 |
SpindlePole | 2 | 0 | 0 | 1 | 5 | 7 | – | 17 | 8 | 9 | 4 | 6 | 5 | 3 | 2 | – | – | 0 | 0 | 2 | 1 | 1 | 1 | 2 |
Vac/Vac Membrane | 103 | 16 | 16 | 11 | 29 | 17 | – | 31 | 29 | 22 | 17 | 11 | 39 | 32 | 66 | – | – | 2 | 22 | 5 | 1 | 7 | 17 | 21 |
Unique Cell Count | 439 | 159 | 291 | 335 | 418 | 467 | 820 | 594 | 745 | 526 | 664 | 362 | 421 | 472 | 16 | 455 | 169 | 301 | 187 | 357 | 407 | |||
Labelled Cell Count | 581 | 197 | 370 | 395 | 510 | 596 | 1018 | 765 | 929 | 663 | 840 | 467 | 544 | 601 | 17 | 455 | 169 | 301 | 187 | 357 | 407 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.5 | 9.0 | 5.3 | 6.6 | 7.1 | 6.3 | – | 6.4 | 5.9 | 6.4 | 6.5 | 6.3 | 8.4 | 8.5 | 8.2 | – | – | 8.4 | 8.4 | 8.9 | 8.6 |
Std Deviation (1e-4) | 1.3 | 1.4 | 1.1 | 1.5 | 1.6 | 1.6 | – | 1.7 | 1.7 | 1.6 | 1.9 | 1.8 | 1.6 | 1.5 | 1.7 | – | – | 2.2 | 2.0 | 2.4 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.31 | 0.42 | 0.25 | – | 0.27 | 0.16 | 0.28 | 0.29 | 0.26 | 0.67 | 0.68 | 0.63 | – | – | 0.67 | 0.66 | 0.75 | 0.71 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud | -2.4 | -2.2 | -2.5 | – | -2.0 | -1.8 | -2.8 | -1.4 | -1.1 | -2.3 | -3.5 | -4.1 | – | – | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Cell Periphery | -0.8 | 0 | -2.8 | – | 0 | 0 | -3.1 | 0 | 0 | -1.2 | -0.7 | -2.0 | – | – | 0 |
Cytoplasm | 0.7 | 1.6 | 1.3 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 0.9 | – | – | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Endosome | 0 | 2.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2.5 | 2.7 | 3.1 | – | – | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.5 | 4.4 | 4.9 | – | – | 0 |
Mitochondria | -0.1 | -3.4 | -3.1 | – | -3.0 | -1.5 | -2.5 | -1.8 | -1.5 | -4.3 | -3.8 | -5.5 | – | – | -13.2 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Nuclear Periphery | 0.6 | 0 | 2.4 | – | 0.9 | 2.9 | 2.8 | 2.6 | 3.1 | -0.8 | -0.4 | -1.7 | – | – | 0 |
Nucleolus | -1.4 | 0.2 | 3.0 | – | 2.2 | 2.4 | 3.0 | 3.2 | 2.0 | 0.5 | -1.3 | -2.5 | – | – | 0 |
Peroxisomes | 0 | 1.2 | 0 | – | 1.0 | 0 | 0 | 0 | 0 | 2.1 | 2.4 | 2.9 | – | – | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 |
Vacuole | -1.4 | 0.8 | -1.2 | – | -1.3 | -0.4 | -2.0 | -1.6 | -3.3 | 2.4 | 1.1 | 3.7 | – | – | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8082 | 4.2159 | 3.7341 | 3.4108 | 3.4168 | 4.1675 | 4.482 | 4.355 | 4.2508 | 4.1103 | 4.6105 | 4.5504 | 5.4649 | 5.696 | 5.5042 | 5.332 | 5.5765 | 5.6421 |
Actin | 0.0047 | 0.002 | 0.0106 | 0.0189 | 0.0653 | 0.001 | 0.0059 | 0.0031 | 0.0104 | 0.0014 | 0.009 | 0.0012 | 0.0109 | 0.0014 | 0.0023 | 0.0013 | 0.0007 | 0.0006 |
Bud | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0009 | 0.0001 | 0.0005 | 0.002 | 0.0005 | 0.0008 | 0.0004 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0 |
Bud Neck | 0.0002 | 0.0001 | 0.0003 | 0.0005 | 0.0005 | 0.0001 | 0.0046 | 0.0003 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0005 | 0.0003 | 0.0004 | 0.0004 | 0.0019 | 0.0002 | 0.001 | 0.0013 | 0.0011 | 0.0065 | 0.0004 | 0.0002 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0001 |
Bud Site | 0.0005 | 0.0002 | 0.0008 | 0.001 | 0.0017 | 0.0001 | 0.0062 | 0.0091 | 0.01 | 0.0002 | 0.0008 | 0.0001 | 0.001 | 0.0006 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0013 | 0.0005 | 0.0005 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0007 | 0.0002 | 0.0006 | 0.0007 | 0.0027 | 0.0001 | 0.0019 | 0.0005 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 |
Cytoplasmic Foci | 0.0178 | 0.0065 | 0.0111 | 0.0143 | 0.0194 | 0.0023 | 0.0227 | 0.0166 | 0.0042 | 0.0009 | 0.0024 | 0.004 | 0.0107 | 0.0113 | 0.0036 | 0.0025 | 0.0044 | 0.0009 |
Eisosomes | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0005 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0006 | 0.0002 | 0.0006 | 0.0007 | 0.0038 | 0.0002 | 0.001 | 0.0003 | 0.0002 | 0.0002 | 0 | 0.0006 | 0.001 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0207 | 0.032 | 0.0201 | 0.015 | 0.0326 | 0.0081 | 0.0423 | 0.0244 | 0.0128 | 0.0014 | 0.0076 | 0.0137 | 0.0231 | 0.0303 | 0.0108 | 0.0016 | 0.0152 | 0.0046 |
Golgi | 0.0217 | 0.0588 | 0.0223 | 0.0144 | 0.0271 | 0.0081 | 0.02 | 0.0315 | 0.013 | 0.0029 | 0.0063 | 0.0114 | 0.0211 | 0.0418 | 0.017 | 0.006 | 0.015 | 0.0129 |
Lipid Particles | 0.0458 | 0.0117 | 0.0116 | 0.025 | 0.0241 | 0.006 | 0.0273 | 0.0108 | 0.0097 | 0.0004 | 0.0017 | 0.0102 | 0.0359 | 0.0144 | 0.0184 | 0.0005 | 0.0019 | 0.0068 |
Mitochondria | 0.8411 | 0.854 | 0.8883 | 0.8842 | 0.7694 | 0.9618 | 0.8069 | 0.8609 | 0.9101 | 0.9469 | 0.9575 | 0.9337 | 0.8371 | 0.853 | 0.9239 | 0.9747 | 0.9514 | 0.9637 |
None | 0.0018 | 0.0001 | 0.0026 | 0.0032 | 0.0014 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0006 | 0.0001 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0012 | 0.0002 | 0.0016 | 0.0009 | 0.0023 | 0.0002 | 0.0089 | 0.0005 | 0.0002 | 0.0001 | 0 | 0.0084 | 0.0122 | 0.0002 | 0.0001 | 0 | 0 | 0 |
Nucleolus | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0012 | 0.0015 | 0.0007 | 0 | 0.0001 | 0.0001 | 0.0005 | 0.0005 | 0.0001 | 0 | 0 | 0.0001 |
Nucleus | 0.0003 | 0.0001 | 0.0007 | 0.0002 | 0.0006 | 0.0001 | 0.0013 | 0.0006 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.003 | 0.0002 | 0 | 0 | 0 | 0 |
Peroxisomes | 0.0291 | 0.019 | 0.0162 | 0.0145 | 0.0324 | 0.0021 | 0.0271 | 0.0247 | 0.0181 | 0.0349 | 0.0112 | 0.0059 | 0.0245 | 0.0317 | 0.016 | 0.0118 | 0.0096 | 0.0078 |
Punctate Nuclear | 0.0033 | 0 | 0.0036 | 0.0025 | 0.0007 | 0.005 | 0.0068 | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0.001 | 0.0001 | 0 | 0 | 0 | 0 |
Vacuole | 0.0036 | 0.0043 | 0.0035 | 0.0008 | 0.0033 | 0.001 | 0.0052 | 0.0058 | 0.0034 | 0.0007 | 0.0008 | 0.0018 | 0.0065 | 0.0062 | 0.0021 | 0.0001 | 0.0005 | 0.0004 |
Vacuole Periphery | 0.0052 | 0.0097 | 0.004 | 0.0018 | 0.0089 | 0.0031 | 0.0081 | 0.0043 | 0.0034 | 0.0017 | 0.0013 | 0.0073 | 0.0092 | 0.0063 | 0.0046 | 0.0005 | 0.0008 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 14.3469 | 23.3186 | 34.6068 | 43.5896 | 27.0565 | 18.2495 | 45.3865 | 52.5134 | 66.8676 | 43.2688 |
Translational Efficiency | 4.3635 | 2.7164 | 1.5827 | 1.7092 | 2.315 | 4.1309 | 1.9462 | 1.6165 | 1.4649 | 1.8269 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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