Standard name | |
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Human Ortholog | |
Description | Beta 5 subunit of the 20S proteasome; responsible for the chymotryptic activity of the proteasome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 4 | 14 | 4 | 3 | 1 |
Bud | 0 | 0 | 0 | 3 | 2 | 2 | 3 | – | 7 | 7 | 8 | 18 | 0 | 0 | 0 | 0 | – | 1 | 0 | 4 | 11 | 5 | 12 | 14 |
Bud Neck | 0 | 3 | 20 | 12 | 18 | 14 | 22 | – | 4 | 4 | 7 | 12 | 0 | 1 | 1 | 0 | – | 0 | 2 | 2 | 6 | 1 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 1 | 4 | 1 | 1 | 1 | – | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 0 | – | 0 | 0 | 2 | 9 | 2 | 3 | 2 |
Cytoplasm | 134 | 412 | 564 | 330 | 311 | 542 | 528 | – | 295 | 305 | 272 | 361 | 197 | 288 | 270 | 18 | – | 32 | 138 | 333 | 367 | 288 | 298 | 277 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 4 | 0 | 0 | 4 | 3 | 4 | 4 |
Endosome | 1 | 0 | 6 | 0 | 0 | 3 | 1 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 2 | 4 | 8 | 3 | 4 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 3 | 1 | 0 | 1 | 0 |
Mitochondria | 19 | 13 | 0 | 3 | 12 | 44 | 43 | – | 146 | 77 | 144 | 151 | 0 | 0 | 0 | 6 | – | 1 | 21 | 26 | 79 | 15 | 19 | 15 |
Nucleus | 0 | 1 | 1 | 1 | 3 | 1 | 4 | – | 2 | 1 | 1 | 5 | 1 | 0 | 0 | 0 | – | 1 | 0 | 1 | 0 | 1 | 2 | 2 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 1 | 1 | 0 | 3 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 7 | 8 | 2 | 16 | 16 | – | 10 | 6 | 16 | 24 | 0 | 0 | 0 | 0 | – | 0 | 2 | 4 | 19 | 9 | 4 | 8 |
Vac/Vac Membrane | 2 | 2 | 5 | 1 | 1 | 2 | 5 | – | 1 | 2 | 3 | 2 | 0 | 0 | 0 | 0 | – | 0 | 3 | 7 | 4 | 3 | 4 | 4 |
Unique Cell Count | 151 | 425 | 586 | 340 | 323 | 588 | 574 | 383 | 357 | 379 | 478 | 197 | 288 | 271 | 23 | 35 | 178 | 409 | 555 | 349 | 373 | 351 | ||
Labelled Cell Count | 160 | 437 | 607 | 362 | 354 | 627 | 625 | 469 | 406 | 453 | 576 | 198 | 290 | 273 | 27 | 39 | 178 | 409 | 555 | 349 | 373 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 3.2 | 5.0 | 4.7 | 4.3 | 3.5 | 4.1 | – | 3.1 | 3.4 | 3.2 | 3.3 | 4.8 | 4.3 | 4.4 | 6.0 | – | 5.4 | 5.3 | 5.6 | 5.7 |
Std Deviation (1e-4) | 0.6 | 0.8 | 2.0 | 2.1 | 1.9 | 1.0 | 1.7 | – | 1.3 | 1.2 | 0.9 | 1.1 | 1.4 | 1.0 | 1.2 | 3.2 | – | 3.1 | 1.7 | 2.1 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.19 | -0.5 | -0.28 | – | -0.7 | -0.56 | -0.61 | -0.59 | -0.04 | -0.2 | -0.16 | 0.28 | – | 0.12 | 0.09 | 0.17 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.481 | 3.4489 | 3.5508 | 3.1572 | 2.9995 | 3.3498 | 3.5263 | 2.9173 | 3.283 | 2.1771 | 3.1995 | 3.1493 | 0.4346 | 0.687 | 0.6404 | 1.0918 | -0.0343 | 0.8079 |
Actin | 0.0319 | 0.0015 | 0.0027 | 0.0044 | 0.0083 | 0.0088 | 0.0178 | 0.0002 | 0.0204 | 0.0003 | 0.0612 | 0.0014 | 0.015 | 0 | 0.0035 | 0.0028 | 0 | 0.0303 |
Bud | 0.0008 | 0.0046 | 0.0022 | 0.0002 | 0.0052 | 0.0007 | 0.001 | 0.0007 | 0.0007 | 0.0041 | 0.0023 | 0.0007 | 0.0015 | 0 | 0.0002 | 0.0088 | 0 | 0.0025 |
Bud Neck | 0.0011 | 0.0013 | 0.003 | 0.0003 | 0.0021 | 0.0003 | 0.0004 | 0.0006 | 0.0012 | 0.0001 | 0.0016 | 0.0002 | 0.0002 | 0 | 0.0016 | 0.0002 | 0 | 0.0012 |
Bud Periphery | 0.0014 | 0.0025 | 0.009 | 0.0003 | 0.0192 | 0.003 | 0.0079 | 0.0009 | 0.002 | 0.0014 | 0.0041 | 0.0028 | 0.0044 | 0 | 0.0001 | 0.016 | 0 | 0.0084 |
Bud Site | 0.0107 | 0.011 | 0.0091 | 0.0008 | 0.0201 | 0.0007 | 0.0014 | 0.0138 | 0.0055 | 0.0009 | 0.0199 | 0.0002 | 0.0035 | 0 | 0.0004 | 0.0009 | 0 | 0.0008 |
Cell Periphery | 0.0008 | 0.0004 | 0.0009 | 0 | 0.0009 | 0 | 0.0009 | 0.0002 | 0.0008 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0256 | 0.0285 | 0.0228 | 0.019 | 0.0493 | 0.0262 | 0.0269 | 0.0336 | 0.0304 | 0.0174 | 0.0094 | 0.0368 | 0.0135 | 0.0097 | 0.0096 | 0.0159 | 0.0258 | 0.0221 |
Cytoplasmic Foci | 0.0392 | 0.0076 | 0.0124 | 0.0169 | 0.0383 | 0.0134 | 0.0168 | 0.0037 | 0.008 | 0.0131 | 0.0302 | 0.005 | 0.0123 | 0.0011 | 0.0171 | 0.0251 | 0.0009 | 0.0043 |
Eisosomes | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0001 | 0.0008 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0068 | 0.0037 | 0.0016 | 0.0001 | 0.0006 | 0.0006 | 0.002 | 0.0002 | 0.0035 | 0.0003 | 0.0011 | 0.0006 | 0.0027 | 0.0001 | 0.0002 | 0.0013 | 0.0003 | 0.0005 |
Endosome | 0.0229 | 0.0065 | 0.008 | 0.0059 | 0.0135 | 0.0101 | 0.0082 | 0.0023 | 0.0081 | 0.0002 | 0.0529 | 0.0116 | 0.0214 | 0 | 0.0037 | 0.0103 | 0.0001 | 0.0023 |
Golgi | 0.008 | 0.0027 | 0.0054 | 0.0249 | 0.0118 | 0.0026 | 0.005 | 0.0009 | 0.0015 | 0.0001 | 0.0414 | 0.0032 | 0.0048 | 0 | 0.005 | 0.0019 | 0 | 0.0023 |
Lipid Particles | 0.0141 | 0.0013 | 0.0034 | 0.0006 | 0.0031 | 0.0021 | 0.0115 | 0.0018 | 0.0028 | 0.0004 | 0.0142 | 0.0084 | 0.0081 | 0.0001 | 0.0137 | 0.0158 | 0.0001 | 0.0009 |
Mitochondria | 0.0123 | 0.0008 | 0.0018 | 0.0632 | 0.0226 | 0.0023 | 0.0058 | 0.0007 | 0.0016 | 0.0009 | 0.0097 | 0.0008 | 0.0073 | 0 | 0.0007 | 0.0031 | 0 | 0.0026 |
None | 0.7686 | 0.9131 | 0.9095 | 0.8529 | 0.7828 | 0.9176 | 0.8395 | 0.9348 | 0.8886 | 0.9544 | 0.7237 | 0.9185 | 0.8661 | 0.9884 | 0.9287 | 0.816 | 0.9724 | 0.9162 |
Nuclear Periphery | 0.0086 | 0.0007 | 0.0008 | 0 | 0.0004 | 0.0015 | 0.0075 | 0.0001 | 0.0028 | 0.0003 | 0.0016 | 0.0016 | 0.0171 | 0 | 0.0002 | 0.0109 | 0.0001 | 0.0005 |
Nucleolus | 0.0043 | 0.0007 | 0.0003 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0011 | 0.0003 | 0.001 | 0.0004 | 0.0003 | 0.0006 | 0 | 0.0005 | 0.002 | 0 | 0.0001 |
Nucleus | 0.0113 | 0.001 | 0.0004 | 0.0001 | 0.0006 | 0.0003 | 0.0273 | 0.0005 | 0.0008 | 0.0013 | 0.0004 | 0.0015 | 0.0061 | 0 | 0.0004 | 0.0441 | 0.0001 | 0.0002 |
Peroxisomes | 0.0136 | 0.0076 | 0.003 | 0.0097 | 0.0143 | 0.0066 | 0.0105 | 0.0014 | 0.0177 | 0.0005 | 0.0209 | 0.0029 | 0.0067 | 0 | 0.0094 | 0.0062 | 0 | 0.0035 |
Punctate Nuclear | 0.0133 | 0.0032 | 0.0004 | 0.0002 | 0.0006 | 0.0017 | 0.0067 | 0.0003 | 0.0009 | 0.002 | 0.0022 | 0.0021 | 0.0051 | 0.0001 | 0.0045 | 0.0159 | 0 | 0.0005 |
Vacuole | 0.0029 | 0.0006 | 0.002 | 0.0001 | 0.004 | 0.0006 | 0.0008 | 0.0019 | 0.0013 | 0.0007 | 0.0014 | 0.0009 | 0.0021 | 0.0001 | 0.0002 | 0.0015 | 0 | 0.0003 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0008 | 0.0001 | 0.0016 | 0.0006 | 0.0009 | 0.0001 | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.001 | 0 | 0.0001 | 0.0009 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 135.3714 | 110.739 | 106.7403 | 169.0048 | 104.3155 | 112.3216 | 138.2286 | 146.0778 | 146.8094 | 149.1005 |
Translational Efficiency | 1.9981 | 2.0491 | 2.3783 | 1.4822 | 1.811 | 3.0161 | 2.0349 | 1.8831 | 1.798 | 1.5674 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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