Standard name
Human Ortholog
Description Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0.1 0.07 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0
Cytoplasm 0.91 0.9 0.86 0.88 0.87 0.86 0.88 0.89 0.78 0.83 0.66 0.76 0.95 0.99 0.97 0.84 0.83 0.82 0.82 0.81 0.66 0.76 0.86 0.82
Endoplasmic Reticulum 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.17 0.12 0.38 0.21 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.09 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.12 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 11 7 9 14 15 20 15 19 6 10 7 7 1 0 1 1 12 9 8 5 3 0 0 1
Bud 2 5 0 3 3 5 11 17 7 9 12 9 3 0 0 0 2 1 2 7 5 0 1 1
Bud Neck 0 1 1 0 2 3 5 5 1 3 3 5 0 0 1 0 0 0 11 8 11 1 1 1
Bud Site 0 0 2 4 2 8 5 6 2 1 6 3 0 0 0 0 1 2 – – – – – –
Cell Periphery 4 11 1 5 2 1 3 2 5 7 5 6 1 5 6 10 13 12 0 0 0 0 0 0
Cytoplasm 188 199 81 174 206 249 375 400 165 208 186 245 113 247 269 133 157 177 177 188 92 43 102 121
Endoplasmic Reticulum 5 8 5 4 4 9 10 1 8 0 12 4 3 0 1 13 15 7 3 2 2 0 0 0
Endosome 1 1 0 1 1 5 1 2 2 0 0 2 0 2 1 3 7 14 5 4 4 0 1 2
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 2 0 0 0 0 0 0 1 0
Mitochondria 5 2 0 0 0 7 19 17 35 31 108 68 1 0 0 6 1 3 2 2 4 0 2 4
Nucleus 0 2 1 1 0 0 4 5 1 0 4 2 3 0 3 2 3 1 0 0 1 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 1 0 0 3 1 3 0 0 0 0 3 4 0 0 0 0 0 0
Peroxisomes 1 0 0 1 2 4 2 2 0 1 1 0 0 0 0 1 1 0 0 0 0 0 0 1
SpindlePole 2 0 0 7 13 10 13 17 5 6 6 11 0 1 2 11 9 20 1 1 1 0 0 0
Vac/Vac Membrane 0 0 0 1 1 0 1 1 1 3 1 3 0 2 1 5 4 4 2 7 9 6 4 9
Unique Cell Count 207 220 94 197 236 290 424 450 212 250 283 323 119 250 276 159 190 215 217 232 139 58 120 148
Labelled Cell Count 219 236 100 215 252 321 465 495 239 282 352 369 125 257 286 187 228 254 217 232 139 58 120 148


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.7 7.0 6.8 7.0 5.7 5.5 5.9 4.9 5.0 4.3 4.7 6.7 7.2 6.9 8.3 8.1 7.7 – 7.0 7.4
Std Deviation (1e-4) 0.7 0.9 0.8 1.4 1.3 1.1 1.1 1.3 1.0 1.1 0.9 0.8 1.1 1.3 1.3 1.1 1.4 1.4 – 1.1 1.4
Intensity Change (Log2) – – – -0.04 -0.01 -0.3 -0.37 -0.26 -0.53 -0.49 -0.73 -0.58 -0.07 0.04 -0.02 0.24 0.21 0.14 – -0.0 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.7 -1.0 -0.9 0 0 0 -2.0 0 0 0 0 0 0 -1.0 -1.9
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0.3 -0.1 0.6 0.7 -1.7 -0.7 -3.8 -2.1 2.2 5.0 4.2 -0.5 -0.8 -0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.9 0.8 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 3.6 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.1
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8838 2.1184 1.8915 1.9854 1.606 1.896 0.7897 1.5385 1.2211 1.5321 1.3116 1.0685 0.9525 1.2489 0.7697 0.7704 0.5669 0.8777
Actin 0.0293 0.003 0.008 0.0018 0.0182 0.0125 0.025 0.0061 0.0142 0.0488 0.003 0.0114 0.0368 0.0035 0.038 0.0001 0.0001 0.0108
Bud 0.0018 0.0045 0.0044 0.002 0.0011 0.0016 0.0008 0.0006 0.0032 0.0005 0.0055 0.0003 0.003 0.0019 0.0028 0.0002 0 0.0007
Bud Neck 0.0181 0.002 0.0084 0.0006 0.0094 0.2871 0.0082 0.0011 0.0015 0.0016 0.0011 0.3266 0.0247 0.0034 0.0013 0.0001 0.0001 0.3619
Bud Periphery 0.0034 0.0029 0.0059 0.0026 0.0017 0.0015 0.0015 0.0005 0.0051 0.0004 0.0102 0.0005 0.0052 0.0013 0.0022 0.0001 0 0.0011
Bud Site 0.0289 0.0308 0.012 0.0012 0.0031 0.0014 0.0125 0.0225 0.0111 0.0021 0.0018 0.0009 0.0461 0.0195 0.0048 0.0001 0 0.005
Cell Periphery 0.0012 0.0009 0.0005 0.0002 0.0004 0.0003 0.0016 0.0009 0.0008 0.0002 0.0005 0.0004 0.0019 0.0013 0.0004 0 0 0.0009
Cytoplasm 0.5463 0.6477 0.6066 0.6915 0.6536 0.4282 0.5143 0.6657 0.5644 0.5499 0.5112 0.41 0.4163 0.7419 0.6708 0.8774 0.6168 0.4741
Cytoplasmic Foci 0.0392 0.0236 0.0196 0.0123 0.0406 0.0606 0.0388 0.0068 0.0191 0.0215 0.0259 0.0619 0.0523 0.0132 0.0159 0.008 0.0501 0.0439
Eisosomes 0.0004 0.0001 0.0002 0 0.0001 0.0001 0.0004 0.0001 0.0001 0.0002 0.0002 0.0001 0.0005 0.0002 0.0006 0 0.0001 0.0001
Endoplasmic Reticulum 0.0053 0.0024 0.004 0.0035 0.0019 0.0014 0.0095 0.0016 0.0038 0.0019 0.0037 0.0025 0.0089 0.0033 0.0028 0.0016 0.0051 0.0021
Endosome 0.0199 0.0093 0.0128 0.006 0.0303 0.0073 0.0235 0.0049 0.0183 0.0146 0.025 0.0127 0.0507 0.0056 0.0094 0.0041 0.0347 0.0063
Golgi 0.0051 0.0006 0.0011 0.0003 0.0072 0.0022 0.0058 0.0009 0.0084 0.0066 0.0073 0.0081 0.0101 0.0013 0.0077 0.0002 0.0007 0.0041
Lipid Particles 0.0053 0.0002 0.0008 0.0001 0.006 0.0056 0.0086 0.0004 0.0035 0.0422 0.0136 0.0047 0.0134 0.0007 0.0048 0 0.0009 0.0054
Mitochondria 0.0051 0.0005 0.002 0.0006 0.0024 0.0011 0.0041 0.0064 0.012 0.0041 0.0032 0.0096 0.0299 0.0113 0.0141 0.0001 0.0001 0.0136
None 0.2554 0.2643 0.2965 0.2685 0.2059 0.1742 0.304 0.277 0.3219 0.2591 0.3581 0.1411 0.2269 0.1874 0.207 0.1038 0.2873 0.0493
Nuclear Periphery 0.0032 0.0007 0.0013 0.0014 0.0017 0.0007 0.0102 0.0002 0.0024 0.0012 0.0008 0.0004 0.0203 0.0004 0.0007 0.0005 0.0008 0.0002
Nucleolus 0.0021 0.0002 0.0005 0.0001 0.0003 0.0005 0.0007 0.0001 0.0003 0.0005 0.0003 0.0001 0.002 0.0004 0.0004 0 0 0.0001
Nucleus 0.0052 0.0021 0.0039 0.0022 0.0013 0.0015 0.0031 0.0007 0.0013 0.0022 0.0008 0.0006 0.0107 0.0007 0.0013 0.0008 0.0004 0.0005
Peroxisomes 0.0126 0.0006 0.0044 0.0003 0.0096 0.0054 0.0139 0.002 0.0031 0.0317 0.0228 0.0048 0.0173 0.0007 0.0101 0 0.0001 0.0154
Punctate Nuclear 0.007 0.0009 0.0012 0.0011 0.003 0.0057 0.0075 0.0002 0.0025 0.0019 0.0006 0.0018 0.0118 0.0003 0.0027 0.0003 0.0007 0.0034
Vacuole 0.0042 0.0027 0.0053 0.003 0.0017 0.0009 0.0047 0.0012 0.0023 0.0077 0.0036 0.001 0.0087 0.0015 0.0018 0.0021 0.0016 0.001
Vacuole Periphery 0.0008 0.0003 0.0006 0.0005 0.0005 0.0002 0.001 0.0001 0.0007 0.0011 0.0009 0.0005 0.0024 0.0001 0.0003 0.0003 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1843 7.5651 6.0455 10.6149 10.9722 14.3135 11.1794 14.6936 11.4013 11.1441
Translational Efficiency 1.1129 0.9984 1.1625 0.6877 0.4927 0.4736 0.8881 0.6248 0.8254 0.6201

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (85%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Dbf20

Dbf20


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dbf20-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available