ORF
Human Ortholog
Description Putative protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0.07 0.06 0.06 0 0.06 – 0.08 0.06 0.09 0.05 0.06 0 0 0.11 0.05 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0.07 0.08 0 0.07 0 0.05 – 0.07 0.11 0.12 0.11 0.14 0.24 0.32 0.33 0.36 0 0 0 0 0 0
Cytoplasm – 0.07 0 0.05 0.06 0.11 0.07 0.06 – 0.11 0.08 0.06 0.05 0.07 0.05 0.27 0.17 0.19 0 0 0 0 0 0
Endoplasmic Reticulum – 0.91 0.88 0.81 0.81 0.67 0.75 0.68 – 0.52 0.54 0.63 0.9 0.87 0.84 0 0 0 0.95 0.93 0.93 0.91 0.89 0.87
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0.05 0.15 0.05 0 0 0 – 0 0 0 0.07 0 0 0.17 0.28 0.36 0 0 0 0 0 0
Mitochondria – 0 0.07 0.06 0.07 0.09 0.22 0.21 – 0.18 0.38 0.35 0 0 0 0 0.1 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0.11 0.09 0.07 0.06 0.13 0.11 – 0.09 0.07 0.1 0 0 0 0.08 0.05 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0.05 0.07 0 0.09 0.14 0 0.11 – 0.23 0.06 0 0 0 0 0.12 0.06 0.09 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 15 9 12 13 16 16 24 – 32 14 30 29 35 26 3 12 5 0 9 2 5 6 6
Bud – 1 2 0 0 3 2 4 – 2 3 5 2 0 0 1 1 0 0 2 0 0 4 4
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 14 17 13 10 19 16 20 – 28 25 37 60 80 140 42 35 34 0 0 1 0 2 5
Cytoplasm – 38 8 8 13 32 27 25 – 44 19 18 24 37 28 35 18 18 0 2 1 4 4 8
Endoplasmic Reticulum – 489 226 137 185 190 279 268 – 216 122 201 475 497 487 3 4 0 30 524 251 392 505 491
Endosome – 0 1 0 1 1 0 0 – 2 0 0 0 0 1 0 0 0 0 2 1 0 1 0
Golgi – 6 12 25 12 1 2 1 – 2 2 2 36 13 15 22 29 34 0 4 1 4 6 6
Mitochondria – 2 18 10 16 25 81 83 – 74 86 110 1 0 0 5 10 4 0 4 4 6 7 7
Nucleus – 1 0 0 0 5 2 1 – 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 22 29 15 16 18 48 43 – 36 16 32 16 14 11 11 5 3 0 3 1 4 3 3
Nucleolus – 0 0 0 0 0 1 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 29 17 6 20 41 14 45 – 95 13 12 14 17 22 16 6 9 0 5 0 9 20 25
Unique Cell Count 537 257 170 227 283 371 393 415 227 318 528 569 580 130 105 95 32 566 269 433 569 566
Labelled Cell Count 617 339 226 286 351 488 514 531 302 447 658 694 732 138 120 107 32 566 269 433 569 566


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 20.8 15.8 15.6 13.5 12.0 11.0 10.9 – 11.4 11.3 11.0 19.4 18.3 18.4 20.1 19.7 20.8 20.8 21.0 20.5
Std Deviation (1e-4) – 3.9 2.7 2.3 2.3 2.0 1.8 1.9 – 2.3 2.3 2.1 3.5 3.5 3.1 4.0 4.4 4.0 3.7 3.8 3.7
Intensity Change (Log2) – – – -0.02 -0.23 -0.39 -0.53 -0.53 – -0.48 -0.48 -0.52 0.3 0.22 0.22 0.34 0.32 0.4 0.4 0.41 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.7 1.2 1.2 0.5 1.5 – 2.2 1.4 2.8 1.2 1.6 0.7 0 2.9 0
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 -1.1 0 -1.3 -0.8 – 0.1 1.7 2.1 2.1 3.1 6.0 6.6 6.6 6.9
Cytoplasm 0.8 1.4 3.6 2.2 1.8 – 3.5 2.5 1.5 1.0 2.0 1.1 7.0 4.7 5.0
Endoplasmic Reticulum -2.1 -2.0 -5.7 -4.0 -5.8 – -9.5 -8.4 -6.7 0.9 -0.2 -1.5 -16.2 -15.1 -15.3
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 3.6 0.3 -3.3 -3.4 -3.9 – -3.7 -2.5 -3.1 1.2 -1.9 -1.6 4.0 6.3 7.7
Mitochondria -0.5 0 0.8 5.0 4.9 – 4.0 8.3 7.9 -5.8 -6.4 -6.4 -1.2 0.8 -1.0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.8 -1.6 -2.0 0.6 -0.1 – -1.1 -1.6 -0.5 -4.7 -5.3 -5.9 -0.9 -1.9 -2.4
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole -1.4 0.9 3.0 -1.6 2.1 – 5.5 -0.4 -1.5 -2.7 -2.4 -1.8 1.9 -0.3 0.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.9666 16.2587 15.3928 14.9454 14.7873 15.0582 21.7766 21.6758 20.9657 20.4236 19.8383 21.3024 22.9771 22.7489 22.1191 21.7792 21.7082 21.3987
Actin 0.0006 0.0021 0.0023 0.0001 0.0002 0.0002 0.014 0.0004 0.005 0.0041 0.0094 0.0002 0.0003 0.001 0.0001 0 0.0001 0.0002
Bud 0 0.0001 0.0002 0 0 0.0001 0.0001 0 0.0001 0.0001 0.0001 0 0 0.0001 0 0 0 0
Bud Neck 0.0001 0.0006 0.0008 0.0002 0.0001 0.0035 0.0004 0.0003 0.001 0.0004 0.0003 0.0002 0.0001 0.0003 0.0002 0 0.0012 0.0003
Bud Periphery 0 0.0001 0.0004 0.0001 0 0.0002 0.0002 0.0001 0.0003 0.0004 0.0001 0 0 0.0003 0 0 0.0001 0
Bud Site 0.0001 0.0008 0.0023 0 0 0.0001 0.0017 0.0002 0.0014 0.0002 0.001 0 0 0.0024 0 0 0 0
Cell Periphery 0.0007 0.0035 0.0014 0.0007 0.0007 0.0008 0.0006 0.0023 0.0014 0.0044 0.0006 0.0004 0.0038 0.0141 0.002 0.0028 0.0044 0.0017
Cytoplasm 0.024 0.048 0.0357 0.0143 0.0129 0.0131 0.0039 0.0184 0.0036 0.002 0.0052 0.001 0.0064 0.0182 0.0132 0.0008 0.0265 0.0028
Cytoplasmic Foci 0.0021 0.0019 0.0007 0.0002 0.0001 0.0002 0.0047 0.0005 0.0004 0.0078 0.0011 0.0002 0 0.0008 0.0002 0 0 0.0001
Eisosomes 0.0001 0 0 0 0 0 0.0002 0 0.0001 0.0005 0.0002 0 0.0002 0.0001 0 0 0 0
Endoplasmic Reticulum 0.8296 0.8846 0.9221 0.9213 0.9259 0.8949 0.8984 0.9594 0.9661 0.9105 0.9564 0.9754 0.913 0.9206 0.9617 0.9843 0.9295 0.9498
Endosome 0.0094 0.0052 0.004 0.0067 0.0034 0.0042 0.0076 0.0028 0.004 0.0144 0.0059 0.002 0.0004 0.0017 0.0008 0.0001 0.0008 0.0006
Golgi 0.0011 0.0026 0.0005 0.0006 0.0002 0.0006 0.0041 0.0021 0.0016 0.0122 0.0018 0.0011 0.0001 0.0007 0.0001 0 0.0001 0.0001
Lipid Particles 0.0023 0.007 0.0017 0.0044 0.0012 0.0011 0.0044 0.0043 0.0012 0.0073 0.0024 0.0017 0.0007 0.0109 0.0011 0.0007 0.0006 0.0012
Mitochondria 0.0004 0.0002 0.0005 0.0004 0.0001 0.0016 0.0094 0.0006 0.0011 0.0008 0.0005 0.0004 0.0001 0.0002 0.0001 0 0.0001 0
None 0.0027 0.0001 0.0004 0.0004 0.0001 0.0001 0.0011 0 0.0001 0.0004 0.0008 0 0.0002 0.0001 0.0005 0 0 0
Nuclear Periphery 0.1052 0.0283 0.0184 0.0307 0.0449 0.0614 0.0341 0.0054 0.0083 0.0237 0.0093 0.0153 0.0631 0.0135 0.0111 0.0098 0.029 0.0372
Nucleolus 0.0001 0 0.0001 0 0 0.0001 0.0004 0 0.0001 0.0001 0.0001 0 0.0002 0.0001 0 0 0 0
Nucleus 0.0119 0.0044 0.0035 0.0039 0.0058 0.0094 0.0042 0.0004 0.0006 0.0006 0.0012 0.0003 0.0067 0.0015 0.0061 0.0002 0.0021 0.0019
Peroxisomes 0.0001 0 0.0005 0 0 0 0.001 0 0.0001 0.0022 0.0001 0 0 0.0001 0 0 0 0
Punctate Nuclear 0.002 0.0002 0.0007 0.0001 0.0002 0.0001 0.0063 0.0001 0.0001 0.0009 0.0005 0 0.0002 0.0005 0.0001 0 0 0
Vacuole 0.0051 0.0096 0.0035 0.0118 0.0035 0.0066 0.0022 0.0025 0.0028 0.0032 0.0026 0.0014 0.0037 0.0107 0.0024 0.0009 0.0049 0.0034
Vacuole Periphery 0.0024 0.0007 0.0004 0.004 0.0008 0.0018 0.001 0.0002 0.0006 0.0041 0.0003 0.0004 0.0008 0.0022 0.0003 0.0002 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 72.7455 62.4289 50.9574 62.5844 61.1179 68.6338 64.9628 69.5848 57.6345 76.6696
Translational Efficiency 0.6191 0.5967 0.7258 0.5363 0.6581 0.733 0.6401 0.489 0.6339 0.5117

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function
Localization
Cell Percentages ER (100%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

YPR114W

YPR114W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypr114w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available