Standard name | |
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Human Ortholog | |
Description | Haploid specific endoprotease of a-factor mating pheromone; performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 128 | 51 | 48 | 34 | 27 | 59 | 30 | 18 | 2 | 10 | 3 | 6 | 38 | 34 | 24 | 29 | 22 | 25 | 54 | 15 | 22 | 4 | 13 | 17 |
Bud | 45 | 22 | 29 | 23 | 32 | 57 | 63 | 70 | 27 | 43 | 50 | 27 | 10 | 9 | 20 | 6 | 4 | 6 | 99 | 27 | 27 | 22 | 53 | 43 |
Bud Neck | 46 | 6 | 26 | 20 | 31 | 35 | 42 | 35 | 14 | 14 | 21 | 12 | 4 | 7 | 3 | 9 | 12 | 4 | 241 | 88 | 79 | 28 | 37 | 21 |
Bud Site | 173 | 54 | 52 | 63 | 44 | 80 | 91 | 58 | 31 | 36 | 40 | 32 | 21 | 23 | 21 | 13 | 9 | 13 | – | – | – | – | – | – |
Cell Periphery | 3 | 3 | 2 | 2 | 2 | 0 | 0 | 3 | 1 | 3 | 3 | 5 | 4 | 1 | 3 | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 2 |
Cytoplasm | 43 | 22 | 21 | 21 | 40 | 64 | 92 | 91 | 65 | 57 | 63 | 80 | 30 | 40 | 56 | 45 | 32 | 37 | 10 | 8 | 5 | 6 | 23 | 57 |
Endoplasmic Reticulum | 4 | 2 | 2 | 0 | 5 | 1 | 5 | 1 | 1 | 1 | 0 | 2 | 1 | 3 | 2 | 7 | 3 | 6 | 12 | 1 | 1 | 0 | 1 | 1 |
Endosome | 7 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 7 | 5 | 7 | 8 | 2 | 4 | 4 | 7 | 0 | 3 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 9 | 51 | 49 | 55 | 31 | 100 | 203 | 185 | 102 | 163 | 141 | 199 | 15 | 1 | 8 | 8 | 2 | 10 | 3 | 6 | 6 | 1 | 3 | 3 |
Nucleus | 38 | 0 | 4 | 1 | 2 | 2 | 15 | 17 | 7 | 5 | 8 | 7 | 0 | 1 | 2 | 1 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 |
Nucleolus | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 60 | 11 | 7 | 22 | 15 | 18 | 30 | 19 | 11 | 15 | 6 | 10 | 32 | 41 | 28 | 13 | 11 | 25 | 11 | 1 | 3 | 1 | 7 | 1 |
SpindlePole | 78 | 6 | 7 | 25 | 24 | 15 | 60 | 65 | 16 | 15 | 18 | 18 | 9 | 20 | 8 | 38 | 30 | 27 | 3 | 2 | 2 | 1 | 6 | 10 |
Vac/Vac Membrane | 3 | 2 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 2 | 2 | 3 | 6 | 5 | 4 | 1 | 1 | 2 |
Unique Cell Count | 380 | 136 | 142 | 148 | 167 | 275 | 423 | 381 | 190 | 241 | 240 | 309 | 104 | 124 | 131 | 127 | 107 | 110 | 461 | 168 | 163 | 68 | 154 | 172 |
Labelled Cell Count | 642 | 230 | 248 | 267 | 255 | 433 | 632 | 568 | 278 | 364 | 357 | 402 | 170 | 187 | 180 | 183 | 142 | 163 | 461 | 168 | 163 | 68 | 154 | 172 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 3.9 | 3.6 | 3.5 | 3.8 | 3.3 | 3.0 | 3.2 | 2.9 | 2.7 | 2.7 | 2.6 | 5.1 | 5.5 | 4.7 | 7.5 | 8.2 | 7.9 | 4.4 | 5.0 | 5.3 |
Std Deviation (1e-4) | 0.7 | 0.6 | 0.6 | 0.5 | 1.1 | 0.8 | 0.7 | 1.0 | 1.2 | 0.9 | 1.0 | 0.9 | 0.9 | 1.8 | 1.5 | 2.6 | 2.8 | 2.6 | 0.4 | 1.9 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.07 | -0.12 | -0.25 | -0.19 | -0.31 | -0.4 | -0.4 | -0.49 | 0.5 | 0.61 | 0.4 | 1.06 | 1.19 | 1.14 | 0.3 | 0.46 | 0.55 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.1772 | 1.7058 | 1.2325 | 1.0899 | 0.6429 | 1.5965 | 1.4029 | 2.2432 | 1.6275 | 1.3273 | 1.2624 | 1.8009 | 2.0489 | 2.6268 | 2.5575 | 2.3545 | 3.2131 | 2.557 |
Actin | 0.0745 | 0.0451 | 0.0738 | 0.0015 | 0.0086 | 0.0731 | 0.1223 | 0.0636 | 0.0792 | 0.0051 | 0.0104 | 0.0548 | 0.0735 | 0.0457 | 0.0432 | 0.0064 | 0.0234 | 0.0611 |
Bud | 0.0011 | 0.0069 | 0.0012 | 0.0001 | 0.0009 | 0.0004 | 0.0017 | 0.002 | 0.0016 | 0.0001 | 0.0009 | 0.0007 | 0.0015 | 0.006 | 0.0011 | 0.0004 | 0.0003 | 0.0004 |
Bud Neck | 0.4443 | 0.0455 | 0.6025 | 0.9945 | 0.9127 | 0.8893 | 0.3093 | 0.041 | 0.542 | 0.8933 | 0.8535 | 0.8955 | 0.2635 | 0.0465 | 0.5867 | 0.9615 | 0.9061 | 0.9132 |
Bud Periphery | 0.0012 | 0.0101 | 0.0024 | 0.0002 | 0.0017 | 0.0009 | 0.0022 | 0.0044 | 0.0031 | 0.0002 | 0.001 | 0.0023 | 0.0027 | 0.0109 | 0.0024 | 0.0009 | 0.0006 | 0.0011 |
Bud Site | 0.3659 | 0.6839 | 0.1958 | 0.0014 | 0.0281 | 0.0194 | 0.3114 | 0.7341 | 0.2242 | 0.0028 | 0.027 | 0.0036 | 0.4712 | 0.6641 | 0.2692 | 0.0283 | 0.0047 | 0.0035 |
Cell Periphery | 0.0034 | 0.0117 | 0.0049 | 0.0004 | 0.0013 | 0.0011 | 0.0029 | 0.0087 | 0.0054 | 0.0004 | 0.0008 | 0.0022 | 0.0057 | 0.0096 | 0.0065 | 0.0008 | 0.0019 | 0.0017 |
Cytoplasm | 0.042 | 0.0961 | 0.0323 | 0.0003 | 0.0034 | 0.002 | 0.031 | 0.0276 | 0.0219 | 0.0006 | 0.0024 | 0.0085 | 0.0159 | 0.1098 | 0.0266 | 0.0003 | 0.0033 | 0.014 |
Cytoplasmic Foci | 0.0139 | 0.017 | 0.0185 | 0.0006 | 0.0149 | 0.0028 | 0.0442 | 0.0046 | 0.0077 | 0.0007 | 0.0269 | 0.0069 | 0.0267 | 0.0177 | 0.0057 | 0.0005 | 0.0017 | 0.0014 |
Eisosomes | 0.0018 | 0.0006 | 0.0012 | 0 | 0.0001 | 0.0004 | 0.0011 | 0.0035 | 0.0009 | 0.0054 | 0.0002 | 0.0005 | 0.0025 | 0.001 | 0.0012 | 0.0001 | 0.0024 | 0.0004 |
Endoplasmic Reticulum | 0.0002 | 0.0025 | 0.0005 | 0 | 0.0001 | 0 | 0.0114 | 0.0005 | 0.0011 | 0 | 0.0001 | 0.0002 | 0.005 | 0.0026 | 0.0005 | 0 | 0.0001 | 0.0001 |
Endosome | 0.0001 | 0.0033 | 0.0018 | 0 | 0.0008 | 0.0001 | 0.0231 | 0.0002 | 0.0134 | 0 | 0.024 | 0.0018 | 0.0117 | 0.0028 | 0.0003 | 0 | 0.0001 | 0.0001 |
Golgi | 0.0006 | 0.0011 | 0.001 | 0.0001 | 0.0019 | 0.0003 | 0.0084 | 0.0008 | 0.0057 | 0.0001 | 0.0229 | 0.0023 | 0.0048 | 0.0019 | 0.0009 | 0.0001 | 0.0003 | 0.0003 |
Lipid Particles | 0.0002 | 0.0017 | 0.0011 | 0 | 0.0026 | 0.0001 | 0.0153 | 0.0005 | 0.006 | 0 | 0.012 | 0.0023 | 0.0095 | 0.0019 | 0.0012 | 0 | 0.0001 | 0 |
Mitochondria | 0.0001 | 0.0006 | 0.0002 | 0 | 0.0003 | 0 | 0.008 | 0.0006 | 0.0077 | 0 | 0.0039 | 0.0029 | 0.0048 | 0.0016 | 0.0003 | 0 | 0.0001 | 0 |
None | 0.0481 | 0.0643 | 0.058 | 0.0002 | 0.0012 | 0.0074 | 0.0526 | 0.0963 | 0.0625 | 0.0905 | 0.0022 | 0.0092 | 0.0725 | 0.0677 | 0.0441 | 0 | 0.0511 | 0.002 |
Nuclear Periphery | 0 | 0.001 | 0.0001 | 0 | 0 | 0 | 0.0129 | 0.0002 | 0.0025 | 0 | 0.0001 | 0.0001 | 0.0056 | 0.0007 | 0.0001 | 0 | 0 | 0 |
Nucleolus | 0.0004 | 0.0008 | 0.0004 | 0.0001 | 0.0037 | 0.0004 | 0.0009 | 0.0004 | 0.001 | 0.0001 | 0.0004 | 0.0025 | 0.0014 | 0.0012 | 0.0009 | 0.0002 | 0.0014 | 0.0001 |
Nucleus | 0.0003 | 0.0021 | 0.0008 | 0.0001 | 0.0028 | 0.0004 | 0.0023 | 0.0007 | 0.0029 | 0.0001 | 0.0009 | 0.0004 | 0.0011 | 0.0021 | 0.0008 | 0.0001 | 0.0011 | 0.0001 |
Peroxisomes | 0.0008 | 0.0014 | 0.0016 | 0 | 0.0126 | 0.0002 | 0.0268 | 0.0082 | 0.0046 | 0.0002 | 0.0087 | 0.0022 | 0.0096 | 0.0027 | 0.0068 | 0.0001 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0007 | 0.0022 | 0.0013 | 0.0002 | 0.0013 | 0.0016 | 0.0089 | 0.0017 | 0.0051 | 0.0002 | 0.0006 | 0.0004 | 0.0087 | 0.0018 | 0.0012 | 0.0001 | 0.0009 | 0.0001 |
Vacuole | 0.0002 | 0.0019 | 0.0003 | 0 | 0.001 | 0.0001 | 0.0017 | 0.0003 | 0.001 | 0 | 0.0009 | 0.0006 | 0.0012 | 0.0017 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Vacuole Periphery | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0013 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0009 | 0.0001 | 0 | 0 | 0 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 10.9519 | 10.3342 | 8.2739 | 17.0958 | 13.2135 | 9.1215 | 11.6176 | 16.1461 | 12.9813 | 16.6105 |
Translational Efficiency | 0.3279 | 0.3198 | 0.5437 | 0.2239 | 0.2564 | 0.4071 | 0.3668 | 0.2403 | 0.3264 | 0.26 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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